C4b (BC067394) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	C4; Ss
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212073 representing BC067394 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTCCTCTGGGGGCTGGCCTGGGTGTTCAGCTTCTGTGCCTCATCCCTGCAGAAGCCCAGGTTGC TCCTGTTTTCCCCTTCTGTGGTTAATTTGGGGACCCCCCTGTCGGTGGGGGTACAGCTCCTGGATGCCCC TCCAGGACAGGAGGTAAAAGGATCAGTGTTCCTCAGAAACCCAAAGGGTGGTTCCTGCTCCCCAAAGAAG GACTTTAAGCTGAGCTCGGGAGATGACTTTGTGCTGCTCAGCCTTGAGGTCCCACTGGAAGATGTGAGGA GCTGTGGCCTCTTTGACCTGCGCAGAGCCCCCCACATCCAGCTGGTAGCTCAGTCTCCGTGGCTAAGGAA CACAGCTTTCAAAGCCACAGAGACTCAGGGTGTCAACTTGCTCTTCTCTTCCCGACGAGGCCACATCTTT GTGCAGACTGATCAGCCTATCTATAATCCAGGGCAGCGGGTTCGTTATCGGGTCTTTGCACTGGATCAAA AGATGCGCCCATCCACTGATTTCCTCACCATCACAGTGGAGAACTCCCATGGCCTCCGTGTACTCAAGAA GGAGATATTTACTTCCACATCCATCTTCCAAGATGCCTTCACCATTCCAGACATCTCAGAGCCTGGGACC TGGAAGATCTCAGCTAGGTTCTCAGATGGACTGGAGTCCAATAGGAGCACCCACTTTGAAGTGAAGAAGT ATGTCCTTCCCAACTTCGAGGTGAAGATTACTCCTTGGAAGCCATATATCCTGATGGTGCCCAGCAACAG TGATGAAATCCAATTAGACATCCAGGCCAGGTACATCTATGGGAAGCCCGTGCAGGGCGTGGCATACACA CGGTTTGCGCTCATGGATGAGCAAGGGAAGAGGACTTTCCTTCGGGGCCTAGAGACGCAGGCCAAGTTGG TGGAAGGCCGGACCCACATTTCCATCTCAAAGGACCAGTTCCAGGCTGCCCTGGATAAAATCAATATTGG GGTCAGAGACCTGGAGGGGCTGCGTCTCTATGCTGCTACAGCTGTCATCGAGTCTCCAGGAGGAGAGATG GAGGAGGCAGAACTCACGTCCTGGCGCTTTGTATCATCTGCCTTCTCCTTGGATCTCAGCCGCACTAAGA GGCATCTTGTGCCTGGGGCCCACTTCCTGCTGCAGGCTCTGGTCCAAGAAATGTCAGGCTCTGAAGCCTC CAACGTTCCTGTTAAAGTCTCTGCCACATTGGTGTCAGGCTCTGACTCCCAAGTCCTTGACATTCAACAG AGCACCAATGGAATTGGCCAAGTCAGCATTTCCTTCCCCATCCCACCAACTGTCACAAAACTTCGACTCT TGGTGTCTGCGGGCTCCCTCTACCCAGCCATAGCCAGGCTCACCGTGCAAGCCCCACCTTCAAGAGGCAC TGGCTTTCTTTCTATTGAGCCACTAGACCCTCGGTCCCCTAGTGTGGGGGACACCTTTATCCTAAACCTT CAACCTGTGGGCATCCCTGCACCTACCTTCTCTCATTACTACTACATGATCATCTCCAGAGGCCAGATCA TGGCTATGGGTCGGGAGCCCCGGAAGACTGTGACCTCCGTCTCTGTGTTGGTGGACCATCAGCTGGCTCC CTCGTTCTACTTTGTGGCTTACTTCTATCACCAAGGACACCCGGTGGCCAACTCTCTGCTCATCAACATC CAATCCAGGGACTGTGAGGGCAAGCTGCAATTGAAGGTGGATGGTGCCAAGGAGTATCGTAATGCGGACA TGATGAAGCTCCGAATTCAAACTGACTCCAAAGCCCTGGTGGCACTGGGAGCTGTGGACACGGCTCTGTA TGCTGTGGGTGGTCGGTCTCACAAACCCCTCGACATGAGCAAGGTCTTTGAAGTAATCAACAGCTACAAT GTTGGCTGTGGTCCTGGAGGTGGGGATGATGCCCTTCAGGTGTTCCAGGATGCTGGTCTGGCCTTTTCTG ATGGTGATCGACTAACTCAAACCAGAGAGGACCTGAGCTGTCCCAAGGAGAAGAAAAGTCGGCAAAAGAG AAATGTTAACTTCCAGAAGGCTGTCAGTGAGAAGTTGGGCCAGTATTCTTCTCCAGATGCCAAGCGCTGC TGCCAAGACGGGATGACGAAGCTGCCCATGAAGCGTACCTGTGAGCAGCGGGCTGCCCGTGTGCCTCAGC AGGCCTGCCGTGAGCCCTTCTTGTCCTGTTGCAAGTTTGCTGAGGACCTTCGCAGGAACCAGACCAGGAG CCAGGCACACCTTGCCCGAAACAACCACAACATGCTGCAGGAGGAAGACTTGATAGATGAAGACGACATT CTTGTGCGCACCTCCTTCCCAGAGAACTGGCTCTGGAGAGTGGAACCTGTAGACAGCTCCAAACTGTTGA CAGTGTGGCTTCCTGATTCTATGACCACATGGGAGATTCATGGTGTGAGCCTGTCCAAAAGCAAAGGTCT GTGTGTAGCCAAGCCAACTCGTGTTCGAGTGTTCAGAAAATTCCACCTGCACCTGCGCCTGCCCATCTCC ATCCGCCGCTTTGAGCAGTTTGAATTACGGCCTGTTCTTTACAACTATCTGAATGATGATGTGGCTGTGA GTGTCCATGTGACCCCAGTTGAGGGGCTGTGCCTGGCTGGTGGTGGAATGATGGCCCAGCAGGTGACAGT GCCAGCAGGTTCTGCCCGGCCTGTGGCCTTCTCTGTGGTACCCACAGCTGCTGCCAACGTGCCCCTGAAG GTGGTGGCTCGAGGGGTTTTTGATTTAGGGGATGCTGTGTCTAAGATTCTCCAAATTGAGAAGGAAGGAG CCATCCACAGAGAAGAGTTAGTCTACAACCTCGACCCCCTAAATAACCTGGGTCGGACTTTGGAGATTCC TGGCAGCTCGGATCCCAACATCGTCCCTGACGGAGACTTCAGCAGCTTAGTCAGGGTTACAGCCTCGGAA CCCTTGGAGACTATGGGTTCTGAAGGTGCCTTGTCCCCAGGAGGTGTGGCCTCCCTCCTGAGGCTTCCCC AGGGCTGTGCAGAGCAAACCATGATCTATTTGGCTCCTACCCTGACTGCTTCCAACTACCTGGACAGGAC AGAACAGTGGAGCAAACTGTCCCCTGAAACAAAGGACCATGCTGTGGATCTGATCCAAAAAGGATACATG AGGATCCAGCAGTTTCGGAAGAATGATGGCTCCTTTGGGGCTTGGTTACACCGGGACAGCAGCACCTGGC TGACTGCCTCCGTGCTGAAGATTCTGAGTTTGGCCCAGGAACAGGTGGGCAACTCCCCGGAGAAGCTGCA GGAGACGGCTAGCTGGCTGCTGGCCCAGCAGCTGGGTGATGGCTCCTTCCACGACCCATGTCCAGTCATC CACAGAGCAATGCAGGGGGGCTTGGTGGGGTCTGACGAGACAGTGGCACTGACCGCCTTTGTGGTCATTG CCCTTCACCATGGGTTGGACGTCTTCCAGGATGACGATGCGAAGCAGCTGAAGAACAGAGTGGAAGCCTC CATCACCAAGGCAAACTCATTCTTGGGGCAGAAGGCAAGTGCTGGGCTCCTGGGTGCCCACGCCGCCGCT ATCACAGCCTATGCCCTTACGCTGACCAAGGCCTCGGAGGACCTGCAGAATGTTGCCCACAACAGCCTGA TGGCCATGGCTGAGGAAACAGGGGAACACCTCTACTGGGGCTTAGTCCTTGGCTCTCAGGACAAAGTTGT GTTGCGCCCCACAGCCCCCCGTAGCCCTACAGAACCTGTGCCCCAGGCCCCAGCCTTGTGGATCGAAACC ACAGCCTATGCCCTGCTCCACCTGCTTCTGCGTGAGGGAAAGGGAAAAATGGCTGACAAGGCTGCATCCT GGCTCACCCACCAGGGAAGCTTCCATGGGGCATTCCGCAGTACCCAGGACACTGTGGTCACCCTGGATGC CCTGTCTGCCTACTGGATCGCTTCACACACCACTGAGGAGAAAGCACTGAACGTGACGCTCAGCTCCATG GGCCGCAATGGGCTTAAGACCCACGGGCTACACTTGAACAACCACCAAGTCAAGGGCCTGGAGGAGGAGC TAAAGTTCTCCCTGGGCAGCACAATCAGTGTCAAGGTGGAAGGAAACAGCAAAGGCACCTTGAAGATCCT TCGTACCTACAATGTCCTGGACATGAAGAACACCACATGCCAGGACCTTCAGATAGAAGTGAAGGTCACA GGCGCTGTGGAATACGCATGGGATGCCAATGAAGACTACGAAGACTACTATGACATGCCAGCCGCAGATG ACCCCAGCGTCCCCTTGCAGCCTGTCACGCCCCTGCAGCTTTTTGAGGGTCGTAGGAGCCGCCGCAGGAG GGAGGCCCCCAAGGTGGTCGAAGAGCAGGAGTCCAGAGTTCAGTACACTGTGTGTATCTGGCGAAATGGC AAGCTGGGGCTGTCTGGCATGGCCATCGCAGACATCACCCTCCTAAGTGGATTCCACGCCCTGAGGGCTG ACCTGGAGAAGCTGACCTCTCTCTCTGACCGTTACGTGAGTCACTTTGAGACTGACGGGCCCCATGTCCT GTTGTACTTTGACTCGGTCCCTACCACCCGGGAGTGTGTGGGCTTCGGAGCCTCGCAGGAGGTGGTTGTG GGACTGGTGCAGCCATCCAGTGCTGTCCTGTATGACTACTACAGCCCTGATCACAAGTGCTCTGTGTTTT ATGCTGCACCCACCAAGAGCCAGCTCCTGGCCACACTGTGCTCTGGAGATGTATGCCAGTGCGCTGAGGG GAAGTGCCCTCGACTGCTAAGGTCACTGGAGCGAAGGGTGGAGGACAAGGACGGCTACCGGATGAGGTTC GCCTGCTATTATCCCCGAGTGGAGTATGGCTTCACGGTTAAGGTTCTTCGAGAAGATGGCAGAGCTGCCT TCCGTCTCTTTGAGTCCAAGATCACCCAAGTCCTGCATTTCAGAAAGGACACCATGGCCTCCATAGGTCA GACCCGCAACTTCCTGAGCCGGGCCTCTTGCCGCCTTCGTTTGGAGCCTAACAAAGAGTACTTGATCATG GGGATGGACGGGGAAACCAGTGACAACAAGGGAGACCCCCAGTACTTGCTGGACTCAAATACCTGGATTG AGGAGATGCCTTCAGAACAAATGTGCAAGAGCACCCGCCATCGGGCAGCCTGTTTCCAGCTCAAAGACTT CCTGATGGAGTTCAGCAGCCGGGGGTGCCAGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212073 representing BC067394 Red=Cloning site Green=Tags(s) MRLLWGLAWVFSFCASSLQKPRLLLFSPSVVNLGTPLSVGVQLLDAPPGQEVKGSVFLRNPKGGSCSPKK DFKLSSGDDFVLLSLEVPLEDVRSCGLFDLRRAPHIQLVAQSPWLRNTAFKATETQGVNLLFSSRRGHIF VQTDQPIYNPGQRVRYRVFALDQKMRPSTDFLTITVENSHGLRVLKKEIFTSTSIFQDAFTIPDISEPGT WKISARFSDGLESNRSTHFEVKKYVLPNFEVKITPWKPYILMVPSNSDEIQLDIQARYIYGKPVQGVAYT RFALMDEQGKRTFLRGLETQAKLVEGRTHISISKDQFQAALDKINIGVRDLEGLRLYAATAVIESPGGEM EEAELTSWRFVSSAFSLDLSRTKRHLVPGAHFLLQALVQEMSGSEASNVPVKVSATLVSGSDSQVLDIQQ STNGIGQVSISFPIPPTVTKLRLLVSAGSLYPAIARLTVQAPPSRGTGFLSIEPLDPRSPSVGDTFILNL QPVGIPAPTFSHYYYMIISRGQIMAMGREPRKTVTSVSVLVDHQLAPSFYFVAYFYHQGHPVANSLLINI QSRDCEGKLQLKVDGAKEYRNADMMKLRIQTDSKALVALGAVDTALYAVGGRSHKPLDMSKVFEVINSYN VGCGPGGGDDALQVFQDAGLAFSDGDRLTQTREDLSCPKEKKSRQKRNVNFQKAVSEKLGQYSSPDAKRC CQDGMTKLPMKRTCEQRAARVPQQACREPFLSCCKFAEDLRRNQTRSQAHLARNNHNMLQEEDLIDEDDI LVRTSFPENWLWRVEPVDSSKLLTVWLPDSMTTWEIHGVSLSKSKGLCVAKPTRVRVFRKFHLHLRLPIS IRRFEQFELRPVLYNYLNDDVAVSVHVTPVEGLCLAGGGMMAQQVTVPAGSARPVAFSVVPTAAANVPLK VVARGVFDLGDAVSKILQIEKEGAIHREELVYNLDPLNNLGRTLEIPGSSDPNIVPDGDFSSLVRVTASE PLETMGSEGALSPGGVASLLRLPQGCAEQTMIYLAPTLTASNYLDRTEQWSKLSPETKDHAVDLIQKGYM RIQQFRKNDGSFGAWLHRDSSTWLTASVLKILSLAQEQVGNSPEKLQETASWLLAQQLGDGSFHDPCPVI HRAMQGGLVGSDETVALTAFVVIALHHGLDVFQDDDAKQLKNRVEASITKANSFLGQKASAGLLGAHAAA ITAYALTLTKASEDLQNVAHNSLMAMAEETGEHLYWGLVLGSQDKVVLRPTAPRSPTEPVPQAPALWIET TAYALLHLLLREGKGKMADKAASWLTHQGSFHGAFRSTQDTVVTLDALSAYWIASHTTEEKALNVTLSSM GRNGLKTHGLHLNNHQVKGLEEELKFSLGSTISVKVEGNSKGTLKILRTYNVLDMKNTTCQDLQIEVKVT GAVEYAWDANEDYEDYYDMPAADDPSVPLQPVTPLQLFEGRRSRRRREAPKVVEEQESRVQYTVCIWRNG KLGLSGMAIADITLLSGFHALRADLEKLTSLSDRYVSHFETDGPHVLLYFDSVPTTRECVGFGASQEVVV GLVQPSSAVLYDYYSPDHKCSVFYAAPTKSQLLATLCSGDVCQCAEGKCPRLLRSLERRVEDKDGYRMRF ACYYPRVEYGFTVKVLREDGRAAFRLFESKITQVLHFRKDTMASIGQTRNFLSRASCRLRLEPNKEYLIM GMDGETSDNKGDPQYLLDSNTWIEEMPSEQMCKSTRHRAACFQLKDFLMEFSSRGCQV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC067394
ORF Size	5216 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC067394, AAH67394
RefSeq Size	5397 bp
RefSeq ORF	5216 bp
Locus ID	12268
Gene Summary	Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. Catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens (By similarity).[UniProtKB/Swiss-Prot Function]

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