Shprh (BC055003) Mouse Tagged ORF Clone

CAT#: MG212063

  • TrueORF®

Shprh (tGFP-tagged) - Mouse SNF2 histone linker PHD RING helicase (cDNA clone MGC:57038 IMAGE:6468603)



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Mouse Protein / Lysate Request

CNY 15,580.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2610103K11Rik; AA450458; AU024614; BC006883; D230017O13Rik; E130018M05
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG212063 representing BC055003
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAGCCGAAGGAAACGTGCTCCCCCGATGAAGGTCGATGAAGAAAGGCAACAGCAGCTTCACTGGA
ACATGCATGAGGACCTGAGGAGTGAGCCGCTCACCATGACGGTCGGGGAACAGGCCTGTTCAGATGCAGA
CTCTTCTTCTGATTGCATCATCATCGATGAAGGCCCTCCTGAGAGTGCACTTCACAGAGATAAGAAGAGG
CGTTCAGAGACTGTGAGCGTCTTAGAGGCCACCGAGGAAGAGACCCGTTTGTCTGTGACTTTGAATGTTA
CAGTTTCTCCCTATCGTGTGGATAATTCTTGGAAAGCATTTCTGGGAGATTTTGCTCTTCAGCTTCTCCC
CAAAGAGAGTTTAGTTGAACATTTTTCTGAAAGGACTTTTACATTGAGTCCTTCAGAGTCAAGCAGTCAG
TTCCTGATCTATGTTCATTCAGAATGTAAAAATGTAGAGAAACAAGAAAATGTTCTTGAAGGATCAGCCG
GTGTTTGTAGCAAGGGAATCCGAGTGGAATCCTCCTTCAGTAGTGACATGTTACAGGATTTGGCCTGGCT
ACAGAAGAGAAGAGGAATAAAACTCTACCAGAGACCAGACGGCACTCACACAATCAAGGTTGGAATTTAC
ATTTTGGAAGCTGGCCTAACAAGACTAGACTTCATGAGTGATGCAGGTTCCAGAATGAAAAAGTTCAATC
AGCTCATGAAGAGAGTGATGGAAAAGTTACATAATTTCATTATTCCAGATGTGCTGGAAGAAGAGGAGGA
GGGTTCAGAGAGCGAGCCCGAGGGTCAGGATATTGATGAGCTCTATCACTTTGTGAAGCAAACACACCAG
CAAGAAACACGGTCAGTCCAAGTGGACGTCCAGCACCCTGCGTTGATTCCGGTCCTGAGACCTTACCAGA
GAGAGGCAGTCAACTGGATGCTGCAGCAAGAGCAGTTCAGAAGCGCTCCTCCTGCTGATAACTCCCTGCA
CTTCTTATGGCGAGAAATTGTTACACCTGATGGCTTGAAACTCTACTATAATCCATATACAGGCTGCATC
ATTCGAGATTTCCCACATGCGGGTCCACAGTTGCTTGGTGGAATCTTAGCAGATGAGATGGGTCTTGGAA
AGACAGTGGAGGTTCTGGCTCTGATTCTGACACATACCCGACAAGACGTCAAGCAGGACGCTCTCACCCT
TCCTGAGGGAAAAGTGGTAAATTATTTCATCCCAACTCACTGTCCTAGAGAAAAAGTGAAAAATAGAGAA
ATCCAGGATACAGAATATGAACCAAAAGAGAAAGTTCACTGCCCTCCTACACGTGTGATGATCCTGACAG
CTGTGAAAGAAATGAATGGGAAGAAAGGAGTTTCTATTCTTTCTATCTATAAGTATGTCAGCTCCATATT
CAGATATGATGTTCAGCGGAACCGGGGTCTTCTGAAGCGGATGTTGAAATGTTTAATCTTTGAAGGTCTT
GTGAAACAGATCAAAGGCCATGGTTTCTCCGGGACTTTTACACTGGGGAAGAATTACAAAGAAGATGTAT
TTGATAAAACAAAAAAGCAGGCAGTAGGGAGTCCAAGGAAAATTGAGAAGGAATTGAGGAAATCAGTGAA
CAAGGATGCAGATTCTGAGTACCTGCCATCAAACACTTCTGATGACGATGAGCCTTACTATTATTACTGT
AAGGCGGGAAAAAGCCGGAGTAAGTTGAAGAAGCCTGCTTTACTAACTAAAAAGGGAAAGGGTCAGTCTG
TTCATCTGGATTCCCAAGGTGATGCTCCAGCTGCTGGAGTCTGTGCAAGTACTGATGTTCATGTGTCAGA
AAATACATGTGTCTCTGAAGACAAGCAAACCCAAGAAGCAAAAGACTGTGCTGAGTCGCCAAACCCTGCT
GCCGAGGAGTTGGCACAGTCTAACACTTCAAGTCCTTGTGAAACCTCTGATTACCGCTTTGAGTGTATAT
GTGGTGAATTTGATCAAATTGGCCACAAGCCAAGAGTTCAATGTCTGAAGTGTCACCTGTGGCAGCATGC
AAAGTGTGTGAATTATGAGGAGAAAAACCTGAAGGTTAAGCCTTTTTACTGCCCCCACTGTCTTGTTGCG
ATGGAGCCAGTATCAACAAGAGCAACTCTGATCATCTCTCCGAGCTCCATTTGCCACCAGTGGGTGGATG
AAATCAATCGGCACGTGAGGTCATCATCTCTCCGAGTTCTGGTATATCAAGGAGTGAAGAAACACGGCTT
CTTACAGCCTCATTTTTTGGCAGAACAAGATATAGTTATCATTACCTATGATGTTCTTCGTTCAGAACTA
AACTATGTCAATATCCCACATAGCAATAGTGAAGATGGGCGTCGCCTGAGGAACCAGAAGCGCTATATGG
CCATTCCCAGCCCCTTGGTAGCGGTGGAATGGTGGAGGATCTGCCTTGATGAAGCTCAGATGGTCGAGTG
TCCTACAGTAAAAGCCGCGGAAATGGCCCAGCGTTTGAGCGGGATTAATCGGTGGTGCATCAGTGGCACT
CCAGTTCAGAGAGGATTGGAAGATCTTTTTGGGCTGGTGGTCTTTCTTGGAATTGAACCTTATTGTGTCA
AACACTGGTGGATTCGGCTTCTCTATCATCCATACTGCAAGAAAAATCCTCAGCACCTGTACAGTTTTAT
TGCCAAGATAATGTGGAGGTCTGCAAAGAAAGATGTCATTGACCAAATCCAGATTCCACCTCAGACTGAA
GAAATGCACTGGCTCCATTTTTCCCCGGTGGAGAGGCATTTCTATCACCGCCAGCACGAGGTGTGCTGCC
AGGATGCCATAGTGAAGCTCAGGAAGATCTCTGACTGGGCCCTGAAGCTCAGCAGCTTGGACAGGAGAAC
TGTCTCCTCCATCCTCTACCCACTGCTGAGGCTCCGGCAGGCCTGCTGCCATCCACAGGCTGTTCGTGGG
GAATTCTTGCCACTCCAGAAGAGCACCATGACAATGGAAGAGTTGCTGACATCTCTGCAGAAGAAATGTG
GAACGGAATGTGAAGAGGCCCACCGACAACTAGTTTGTGCCCTTAACGGCTTAGCAGGCATTCACATCAT
TAAAGGTGAATATGCCTTGGCAGCGGAGCTCTACAGAGAAGTATTACGTTCATCTGAGGAGCACAAAGGA
AAACTCAAAACTGATTCCCTTCAAAGACTGCATGCCACACATAACTTAATGGAGCTGTTGGGAGCCAAAC
ACCCAGGAATACCTCCCACCTTGAGAGATGGCAGACTTGAAGAAGAGGCCAAGCAGCTGCGAGAGCACTA
TATGAGTAAGTGTAACACAGAAGTTGCCGAAGCCCAGCAAGCTTTGCAGCCTGTGCAGCAGTCCATTAGG
GAGCTCCAAAGGAAGATTCATTCTAATTCTCCTTGGTGGCTGAATGTAATCCATAGAGCAATGGAATTTT
CTGTTGATGAGGAACTTGTTCAGCGGGTGCGAAATGAAATAAGCAGTAACTACAAGCAACAAACTGACAA
GCTGTCTATGTCAGAGAAGTTCCGTGACTGCAGAGGTCTTCAGTTCTTACTTACAACACAAATGGAAGAG
CTCCATAAGTTCCAGAAGCTGGTGAGGGAGGCTGTGAAGAAGCTGGAGAAGCCTCCATCCCGGGAGGTGA
TTGAGTCTGCAACAGTTTGCCACCTGCGGCCAGCTAGACTCCCTCTCAACTGCTGCGTCTTTTGTAAAGC
TGATGAATTGTTCACAGAGTATGAATCAAAGCTATTTTTTAACACAGTCAAAGGCCAGACTGCAATCTTT
GAAGAAATGATAGAAGATGAAGAAGGACTTGTAGATGATAGAGTACCTACCACTACCAGAGGTCTGTGGG
CCGTAAGTGAGACGGAGCGATCTATGAAAGCAATATTATCATTTGCAAGATCACATAGGTTTGATGTTGA
ATATGTAGATGAAGGAAGTGTTTCAATGGATCTCTTTGAGGCATGGAAGAAGGAATATAAGTTACTTCAT
GAATACTGGATGACTCTGAGAAATCGTGTATCTGCTGTTGATGAACTTGCAATGGCTACAGAGCGACTAA
GAGTGCGCCATCCCAAAGAGCCAAAGCCAAACCCACCCGTCCATCACATCATTGAACCACACGAGGTTGA
ACAGAATCGTATAAAACTGGTGAATGATAAAGCTGTTGCTACATCACAGCTTCAGAAGAAACTTGGACAG
CTTCTTTACCTAACGAATTTGGAGAAGTCTCAAGATAAAACATCAGGAGGTATTAATCCAGAGCCTTGTC
CAATCTGTGCTCGACAGCTGGGAAAACAGTGGGCAGTGCTGACCTGCGGACACTGCTTCTGTAATGAATG
CACTTCGATTATAATTGAACAGTACAGTGTGGGGTCTCATCGAAGTTCCATCAAGTGTGCTATCTGCCGC
CAGACCACATCACACAAAGAAGTCTCCTATGTCTTCACCTCAGAGAAGGCAAACCAAGAGGATGATATCC
CTGTGAAGGGTAGCCATTCTACAAAAGTGGAAGCTGTGGTCAGAACTCTGATGAAAATACAACTTAGAGA
TCCAGGGGCCAAAGCGCTCGTTTTCTCAACGTGGCAAGATGTATTAGATATTATTTCAAAAGCTCTCACG
GACAACAACATGGAATTTACACAAATCAGTCGCATTAAGACATTCCAGGAGAACCTCTCAGCGTTTAAAT
ACGATCCCCACATCAATATTCTGCTGCTGCCCCTGCACACAGGCTCTAATGGATTAACTATCATTGAAGC
CACCCATGTCCTCCTGGTGGAGCCCATACTGAACCCTGCCCATGAGCTGCAGGCCATTGGGAGAGTGCAC
CGAATTGGACAGACCAAACCTACTATTGTACATAGATTCTTAATTAAAGCAACAATAGAAGAACGGATGC
AGGCAATGCTAAAAACTGCTGAGAGGAGCCACACAAGCTCATCCGGAAAGCATTCTGAAGCCTCTGTGTT
GACTGTGGCTGGCCTGGCTGACCTGTTTACCAAGGAGAACGAGGAGCTTGAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG212063 representing BC055003
Red=Cloning site Green=Tags(s)

MSSRRKRAPPMKVDEERQQQLHWNMHEDLRSEPLTMTVGEQACSDADSSSDCIIIDEGPPESALHRDKKR
RSETVSVLEATEEETRLSVTLNVTVSPYRVDNSWKAFLGDFALQLLPKESLVEHFSERTFTLSPSESSSQ
FLIYVHSECKNVEKQENVLEGSAGVCSKGIRVESSFSSDMLQDLAWLQKRRGIKLYQRPDGTHTIKVGIY
ILEAGLTRLDFMSDAGSRMKKFNQLMKRVMEKLHNFIIPDVLEEEEEGSESEPEGQDIDELYHFVKQTHQ
QETRSVQVDVQHPALIPVLRPYQREAVNWMLQQEQFRSAPPADNSLHFLWREIVTPDGLKLYYNPYTGCI
IRDFPHAGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKVVNYFIPTHCPREKVKNRE
IQDTEYEPKEKVHCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSIFRYDVQRNRGLLKRMLKCLIFEGL
VKQIKGHGFSGTFTLGKNYKEDVFDKTKKQAVGSPRKIEKELRKSVNKDADSEYLPSNTSDDDEPYYYYC
KAGKSRSKLKKPALLTKKGKGQSVHLDSQGDAPAAGVCASTDVHVSENTCVSEDKQTQEAKDCAESPNPA
AEELAQSNTSSPCETSDYRFECICGEFDQIGHKPRVQCLKCHLWQHAKCVNYEEKNLKVKPFYCPHCLVA
MEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKHGFLQPHFLAEQDIVIITYDVLRSEL
NYVNIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGT
PVQRGLEDLFGLVVFLGIEPYCVKHWWIRLLYHPYCKKNPQHLYSFIAKIMWRSAKKDVIDQIQIPPQTE
EMHWLHFSPVERHFYHRQHEVCCQDAIVKLRKISDWALKLSSLDRRTVSSILYPLLRLRQACCHPQAVRG
EFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKG
KLKTDSLQRLHATHNLMELLGAKHPGIPPTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALQPVQQSIR
ELQRKIHSNSPWWLNVIHRAMEFSVDEELVQRVRNEISSNYKQQTDKLSMSEKFRDCRGLQFLLTTQMEE
LHKFQKLVREAVKKLEKPPSREVIESATVCHLRPARLPLNCCVFCKADELFTEYESKLFFNTVKGQTAIF
EEMIEDEEGLVDDRVPTTTRGLWAVSETERSMKAILSFARSHRFDVEYVDEGSVSMDLFEAWKKEYKLLH
EYWMTLRNRVSAVDELAMATERLRVRHPKEPKPNPPVHHIIEPHEVEQNRIKLVNDKAVATSQLQKKLGQ
LLYLTNLEKSQDKTSGGINPEPCPICARQLGKQWAVLTCGHCFCNECTSIIIEQYSVGSHRSSIKCAICR
QTTSHKEVSYVFTSEKANQEDDIPVKGSHSTKVEAVVRTLMKIQLRDPGAKALVFSTWQDVLDIISKALT
DNNMEFTQISRIKTFQENLSAFKYDPHINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVH
RIGQTKPTIVHRFLIKATIEERMQAMLKTAERSHTSSSGKHSEASVLTVAGLADLFTKENEELE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC055003
ORF Size 5024 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC055003, AAH55003
RefSeq Size 6977 bp
RefSeq ORF 5024 bp
Locus ID 268281
Gene Summary E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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