Jmjd3 (BC075632) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mKIAA0346, RP23-5O23.5, 1700064E03Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212060 representing BC075632 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCGGGCAGTGGACCCTCCAGGGGCCCGCTCTGCACGGGAAGCCTTTGCCCTTGGGGGCTTGAGCT GTGCTGGGGCTTGGAGCTCCTGCCCACCCCATCCTCCTCCCCGAAGCTCATGGCTGCCCGGAGGCAGATG CTCTGCCAGCGTTGGGCAGCCCCCACTCTCAGCTCCTTTACCCCCATCTCATGGCAGTAGCTCCGGGCAC CCTAACAAACCCTATTATGCTCCTGGGACACCCACCCCAAGACCCCTTCACGGGAAGTTGGAATCCCTAC ATGGCTGTGTCCAGGCATTGCTCCGGGAGCCAGCGCAGCCAGGGTTGTGGGAACAGCTTGGACAGTTGTA TGAATCAGAGCACGACAGTGAGGAAGCCGTATGCTGCTACCATAGGGCCCTTCGCTATGGAGGAAGCTTC GCCGAGCTGGGACCCCGGATTGGCCGCTTGCAGCAGGCCCAGCTCTGGAACTTTCATGCCGGTTCCTGTC AGCACAGAGCCAAGGTCCTGCCTTCCCTGGAGCAAGTCTGGAATTTGCTGCACCTTGAGCACAAACGGAA CTATGGGGCTAAGCGAGGGGGCCCTCCAGTGAAGAGATCTGCTGAACCCCCCGTGGTCCAGCCTATGCCT CCTGCAGCCCTCTCAGGCCCCTCAGGAGAGGAGGGCCTTAGCCCTGGAGGCAAGCGCAGGAGAGGCTGCA GCTCTGAACAGGCTGGCCTTCCCCCAGGTCTGCCACTCCCTCCACCACCCCCACCCCCACCGCCTCCACC ACCACCACCACCCCCTCCACCACCACCGCTGCCTGGCCTGGCTATTAGCCCCCCATTTCAGCTGACTAAG CCAGGGCTGTGGAATACCCTGCATGGAGATGCTTGGGGCCCCGAGCGCAAGGGTTCAGCGCCGCCAGAGC GCCAGGAGCAGCGGCACTCGATGCCTCATTCATATCCATACCCAGCTCCCGCCTACTCCGCTCATCCGCC CAGCCATCGGCTGGTCCCCAACACACCCCTTGGTCCAGGTCCCCGACCCCCAGGAGCAGAGAGCCATGGC TGCCTGCCTGCCACCCGTCCCCCCGGAAGTGACCTTAGAGAGAGCAGAGTTCAGAGGTCGCGGATGGACT CCAGCGTTTCACCAGCAGCATCTACCGCCTGCGTGCCTTACGCCCCTTCCCGGCCCCCTGGCCTCCCCGG CACCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGTAACAACACTGGTCTTCGGGGTGTGGAGCCAAGCCCA GGCATTCCTGGCGCTGACCATTACCAAAACCCTGCGCTGGAGATATCCCCTCACCAGGCCCGCCTGGGTC CCTCCGCACACAGCAGTCGGAAACCATTTTTGACGGCCCCTGCTGCCACGCCCCACTTATCCCTACCCCC TGGGACCCCATCATCCCCTCCACCCCCATGTCCTCGCCTCTTGCGCCCTCCACCGCCCCCTGCTTGGATG AAGGGCTCAGCCTGCCGTGCAGCCCGAGAGGATGGAGAGATCTTAGGGGAGCTCTTCTTTGGTGCTGAGG GACCTCCCCGTCCTCCTCCCCCACCCCTTCCCCACCGTGATGGCTTCTTGGGGCCTCCAAACCCCCGCTT TTCTGTGGGCACTCAGGATTCGCATAACCCTCCCATTCCCCCAACCACCACCAGCAGCAGCAGCAGCAGC AACAGCCACAGCAGTAGTCCTACTGGGCCGGTGCCCTTTCCACCACCCTCCTATCTGGCCAGAAGTATAG ACCCCCTCCCCAGGCCATCCAGCCCAACCTTGAGCCCCCAGGACCCACCTCTTCCACCACTGACTCTTGC CCTGCCTCCAGCCCCTCCCTCCTCCTGCCACCAAAATACCTCAGGAAGCTTCAGGCGCTCGGAGAGCCCC CGGCCCAGGGTCTCCTTCCCAAAGACCCCCGAGGTGGGGCAGGGGCCACCCCCAGGCCCTGTGAGTAAAG CCCCCCAGCCTGTGCCACCTGGGGTTGGAGAGCTGCCTGCCCGAGGCCCGAGGCTCTTTGATTTCCCACC CACTCCGCTGGAGGACCAGTTTGAAGAGCCAGCCGAATTCAAGATCCTACCTGATGGGCTGGCAAACATC ATGAAGATGCTGGATGAATCCATTCGGAAGGAGGAGGAGCAGCAGCAGCAGCAGGAGGCAGGCGTGGCTC CCCCACCCCCACTCAAAGAGCCCTTTGCATCTCTACAGCCTCCATTTCCCAGTGACACAGCCCCAGCCAC CACCACTGCTGCCCCCACCACCGCCACCACCACCACAACCACCACCACCACCACCACCCAAGAAGAGGAG AAGAAGCCACCACCAGCCCTACCACCACCACCGCCTCTAGCCAAGTTTCCTCCACCTCCCCAGCCACAGC CCCCACCACCTCCACCAGCCAGCCCAGCCAGCCTGCTCAAATCGTTGGCCTCTGTTCTTGAGGGACAAAA GTACTGTTACCGGGGGACTGGAGCAGCCGTCTCAACCAGGCCCGGGTCCGTGCCCGCCACTCAGTATTCC CCTAGTCCTGCATCAGGTGCTACCGCCCCACCACCCACTTCAGTGGCCCCTAGTGCCCAGGGCTCCCCCA AGCCCTCGGTTTCCTCGTCATCTCAGTTCTCTACCTCAGGCGGGCCTTGGGCCCGGGAGCACAGGGCGGG TGAAGAGCCAGCACCAGGCCCCGTGACCCCTGCCCAGTTGCCCCCACCTCTGCCGCTGCCCCCTGCTCGT TCTGAGTCTGAGGTGCTAGAAGAAATCAGTCGGGCTTGTGAGACCCTTGTAGAGCGGGTGGGCCGGAGTG CCATCAACCCAGTGGACACGGCAGACCCAGTGGACAGTGGGACTGAGCCACAGCCGCCGCCTGCGCAGGC CAAGGAGGAGAGTGGGGGGGTGGCGGTAGCAGCAGCAGGTCCAGGTAGTGGCAAGCGTCGTCAAAAGGAG CATCGGCGGCACAGGCGGGCCTGTAGGGACAGTGTGGGTCGACGACCCCGCGAGGGGAGGGCCAAGGCCA AGGCCAAGGCTCCCAAAGAAAAAAGCCGAAGGGTGCTGGGGAACCTCGACTTGCAGAGTGAGGAGATCCA GGGCCGGGAGAAGGCCCGGCCCGATGTCGGTGGGGTTTCCAAAGTCAAGACACCCACAGCTCCAGCACCC CCGCCTGCTCCTGCACCCGCTGCTCAGCCAACACCCCCATCAGCTCCTGTCCCTGGGAAGAAGACTCGTG AGGAGGCTCCGGGGCCTCCAGGTGTGAGCCGGGCAGATATGCTGAAGCTCCGGTCACTTAGTGAGGGGCC TCCCAAGGAGCTGAAGATCAGGCTCATCAAGGTGGAAAGTGGGGACAAGGAGACCTTTATCGCCTCTGAG GTGGAAGAGCGGCGGCTGCGCATGGCAGACCTCACCATCAGCCACTGTGCCGCCGATGTCATGCGTGCCA GCAAGAATGCCAAGGTGAAAGGGAAATTCCGAGAGTCCTACCTGTCCCCTGCCCAGTCTGTGAAACCCAA GATCAACACTGAGGAGAAGCTGCCCCGGGAAAAACTCAACCCCCCTACCCCCAGCATCTATTTGGAGAGC AAACGAGATGCCTTCTCGCCGGTCCTGCTACAGTTCTGTACAGACCCCCGGAACCCCATCACCGTCATCA GGGGCCTGGCTGGTTCACTTCGGCTCAACTTAGGCCTTTTCTCCACCAAGACTCTGGTGGAGGCGAGCGG TGAACATACGGTGGAGGTCCGTACCCAAGTACAGCAGCCCTCAGACGAGAACTGGGACCTGACAGGTACC AGACAAATCTGGCCCTGTGAGAGCTCCCGTTCCCACACCACCATCGCTAAATACGCACAGTACCAGGCCT CGTCCTTCCAGGAGTCACTGCAGGAGGAGAGGGAGAGTGAGGATGAGGAATCCGAGGAACCAGACAGCAC TACAGGAACCTCTCCCAGCAGTGCACCGGACCCCAAGAACCATCACATCATCAAGTTTGGCACTAACATC GACCTGTCTGATGCCAAGAGGTGGAAGCCACAGCTACAGGAGCTGCTGAAACTGCCCGCCTTCATGCGGG TAACATCCACAGGCAACATGCTCAGCCACGTGGGCCACACCATCCTGGGCATGAACACCGTGCAGCTATA CATGAAGGTCCCTGGCAGCCGAACGCCAGGCCACCAAGAGAATAACAATTTCTGCTCAGTCAACATCAAC ATTGGCCCTGGGGACTGCGAGTGGTTCGCGGTACATGAGCACTATTGGGAGACCATCAGCGCCTTCTGCG ACCGGCATGGTGTGGACTACTTGACTGGTTCCTGGTGGCCAATCTTGGATGACCTCTATGCGTCCAATAT TCCTGTTTACCGCTTCGTGCAGCGCCCTGGAGACCTTGTGTGGATTAATGCAGGGACTGTACATTGGGTG CAGGCTACCGGCTGGTGCAACAACATTGCCTGGAACGTGGGGCCCCTCACCGCCTATCAGTACCAGCTGG CCCTGGAGCGATATGAGTGGAACGAGGTGAAGAACGTCAAGTCCATTGTGCCCATGATTCATGTGTCCTG GAACGTCGCTCGAACGGTCAAGATCAGCGATCCTGACTTGTTCAAGATGATCAAGTTCTGCCTCCTGCAG TCAATGAAGCACTGTCAGGTACAGCGGGAGAGCCTGGTGCGGGCAGGGAAGAAGATCGCTTACCAAGGCC GTGTCAAAGACGAGCCTGCCTACTACTGCAACGAATGCGACGTGGAGGTGTTCAACATCCTGTTCGTTAC AAGTGAGAATGGCAGCCGAAACACGTACCTGGTGCACTGCGAGGGCTGTGCGCGCCGTCGCAGCGCGGGC CTACAGGGCGTGGTGGTGCTAGAGCAGTACCGCACGGAGGAGCTGGCGCAGGCCTACGATGCCTTCACAC TGGCTCCCGCCAGCACGTCTCGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212060 representing BC075632 Red=Cloning site Green=Tags(s) MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGH PNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSF AELGPRIGRLQQAQLWNFHAGSCQHRAKVLPSLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMP PAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTK PGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHG CLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSP GIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWM KGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSS NSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESP RPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANI MKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEE KKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYS PSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPAR SESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKE HRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAP PPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASE VEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLES KRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGT RQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNI DLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNIN IGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWV QATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQ SMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAG LQGVVVLEQYRTEELAQAYDAFTLAPASTSR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC075632
ORF Size	4925 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC075632, AAH75632
RefSeq Size	5680 bp
RefSeq ORF	4925 bp
Locus ID	216850
Gene Summary	Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (PubMed:21095589).[UniProtKB/Swiss-Prot Function]

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