Arhgef11 (BC079565) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	B930073M02, E130307F09, RhoGEF, mKIAA0380
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212032 representing BC079565 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATACGGTTGCCCCATAGTATAGACAGGTTAAGTAGCCTGTCTTCTCTGGGAGATTCTACACCTG AACGCACATCCCCTTCTCACCACCGCCAGCCCTCTGACACCTCTGAGACAACAGGTCTTGTTCAGCGCTG TGTCATCATCCAAAAGGACCAGCATGGCTTTGGCTTCACAGTTAGTGGAGATCGCATTGTGCTGGTGCAG TCTGTGCGCCCTGGAGGCGCAGCCATGAGAGCTGGTGTGAAAGAGGGTGACCGGATCATCAAAGTAAACG GCACCATGGTGACCAATAGCTCACACCTGGAGGTAGTGAAGCTTATCAAATCTGGCGCCTATGTTGCGCT TACCCTCCTGGGCTCTTCTCCTTCCTCCATCGGTGTCTCTGGGCTCCAGCAGAACCCTTCTCTGTCAGGA GGGCCCAGAGTTAACCCCATGATTCCTCCACCACCTCCTCCTCCACCCTTGCCACCTCCACAACACATTA CTGGACCTAAACCTCTACAGGATCCTGAAGTCCAAAAGCACGCCACTCAAATCCTCAGGAATATGCTAAG GCAGGAGGAGAAGGAGTTACAGCGTATCTGTGAGGTGTATAGTCGGAACCCCGCCAGCCTGCTAGAAGAG CAAATCGAAGGTGCCCGGCGGCGAGTCACTCAATTACAGCTGAAGATCCAGCAGGAGACTGGTGGCTTAG TGGATGTACTTCCACTCTGTGGTGAGACCAGCCAGAGAACATGTGAAGGCCGCCTCTCTGTGGACTCCCA GGAGGCAGACAGTGGCTTGGACTCAGGGACAGAACGCTTTCCCTCCATCAGTGAGTCATTGGTGAATCGG AACTCAGTATTGTCAGATCCTGGACTAGACAGCCCACAAACCTCCCCTGTAATCCTGGCCAGGGTGGGCC AGCACCACAGGCGACAGGGCTCAGATGCAGCGGTGCACCCCCTCAACCACCAGGGTATAGATCAAAGCCC AAAGCCTCTGATTATTGGCCCAGAGGAAGATTATGACCCAGGCTATTTCAACAATGAGAGTGACATCATC TTTCAAGATCTTGAAAAACTGAAGTCACATCCAGCTTACTTGGTAGTATTTCTACGTTACATCTTCTCTC AGGCAGACCCCGGCCCCCTGCTTTTTTATTTGTGTTCAGAAGTTTATCAGCAGACAAATCCCAAAGATTC CCGAAATCTGGGGAAAGACATCTGGAATATTTTCCTAGAGAAAAATGCGCCTCTCAGAGTGAAGATCCCT GAGATGTTGCAGGCTGAGATCGACCTACGCCTGCGAAACAGTGAGGACCCTCGCAGCGCGCTTTATGAAG CTCAGGAGGCAGTCATGCCGGAGATCCAGGAGCAGATCAATGACTATAGATCAAAGCGCACTCTGGGGCT GGGCAGCCTCTATGGTGAAAATGACCTACTAGACCTGGATGGGGACCCTCTTCGAGAGCGCCAAATGGCT GAGAAGCAACTGGCTGCCCTTGGAGATATCTTGTCCAAATATGAGGAAGATCGGAGTGCTCCCATGGACT TCGCTGTTAATACCTACATGAGCCACACTGGGATCCGTCTTCGGGAGTCTCGACCTTCCAGCACAGCAGA AAAGACCCAGTCTGCCCCCGACAAGGACAAGTGGCTGCCCTTCTTCCCTAAGACCAAGAAGAGCAGCAAT TCCAAGAAAGAAAAGGACGCCTTGGAGGACAAGAAGCGAAACCCCATCCTCAGATACATTGGGAAGCCCA AGAGCTCTTCTCAGAGCATTAAGCCAGGCAATGTGAGGAACATCATTCAGCACTTTGAGAACAGCCATCA GTATGATGTCCCAGAGCCGGGGACACAGCGACTCTCAACAGGAAGCTTTCCTGAGGACCTGCTGGAGAGT GACAGTTCACGCTCAGAGATTCGCCTTGGCCGCTCTGAAAGCCTCAAGGGCCGGGAGGAGATGAAGCGAT CCCGGAAGGCAGAGAATGTGCCCCGGTCTCGAAGTGATGTTGACATGGATGCTGCTGCTGAGGCTACTCG CCTTCATCAGTCAGCCTCATCCTCTGCCTCCAGCCTCTCCACCAGGTCTCTTGAGAACCCAACCCCTCCC TTCACCCCCAAAATGGGCCGCAGGAGCATTGAGTCCCCCAATCTGGGGTTCTGTACAGATGTCATCCTTC CCCACCTCCTTGAGGATGATCTGGGCCAACTGTCTGACCTGGAGCCAGAGCCAGAAGTCCAAAACTGGCA GCATACAGTGGGCAAGGATGTGGTGGCAAACTTGACACAGAGGGAAATTGACCGGCAAGAGGTCATCAAT GAGCTCTTTGTGACAGAAGCATCCCACCTGCGCACGCTACGAGTCCTGGACCTCATCTTCTACCAGCGCA TGAAGAAGGAGAACCTAATGCCACGGGAAGAGCTGTCAAGGCTCTTCCCTAATCTGCCTGAACTCATAGA GATCCACAATTCCTGGTGTGAAGCCATGAAGAAGCTCCGGGAGGAGGGCCCCATTATCAGAGACATCAGT GACCTCATGCTGGCTCGGTTTGATGGTCCTGCCCGAGAAGAGCTCCAGCAAGTAGCTGCACAATTCTGTT CCTATCAGTCAGTAGCCCTGGAGCTAATCAGGACTAAACAACGCAAGGAGAGTCGATTCCAGCTCTTCAT GCAGGAAGCCGAGAGCCACCCCCAGTGTCGGCGTCTGCAGCTTCGTGACCTGATCATCTCTGAGATGCAG CGGCTCACTAAGTACCCACTGCTGCTAGAGAACATCATCAAGCACACAGCGGGTGGCACATCTGAGTATG AGAAGCTCTGCCGTGCCCGGGACCAGTGCCGAGAGATTCTCAAGTTTGTGAATGAAGCAGTGAAGCAGAC AGAGAACCGTCACCGCTTAGAAGGGTACCAGAAACGCCTGGATGCCACTGCTCTAGAGCGGGCCAGCAAC CCCTTGGCAGCAGAGTTCAAGAGCCTGGATCTTACGACAAGGAAGATGATCCACGAGGGGCCTCTGACCT GGAGGATCAGCAAAGATAAGACCCTGGACCTCCAGGTGCTGTTACTCGAGGACCTGGTAGTGCTGCTGCA GAGACAAGAGGAGAAGCTGCTGCTCAAGTGCCACAGCAAGACAGCTGTGGGCTCCTCAGACAGCAAGCAG ACCTTCAGCCCTGTGCTGAAGCTCAATGCCGTGCTCATCCGCTCTGTGGCCACAGATAAACGAGCCTTCT TCATCATCTGCACCTCCGAGCTGGGCCCTCCCCAGATCTATGAGCTGGTTGCATTGACATCATCAGACAA GAATATATGGATGGAGCTCTTAGAAGAGGCTGTGCAGAATGCCACCAAGCACCCTGGAGATGCCCCAGTA CTCAACCATCCCTCACCACCAGGATCCCAGGAGCCAGCCTACCAGGGCTCCACCTCCAGCAGGGTAGAGG TGAATGACTCGGAAGTGTATCCCACTGAAAGAGAACCCAAGAAGCCATCTGAAGGCCCCGGGCCCGAGCA GAGAGGTCAAGACAAGCAGCTGCTAGCACAAGAGGGGCCTGAGCAGGAGGAGGATGCAGAGGAGCTGAGG GCCCTGCCTTGCCCTCCTCCCTCCCTGGATGGAGAAAACAGAGGCATCAGGACAAGGGACCCTGTCCTTC TGGCCCTCACAGGCCCTCTGCTCATGGAGGGACTTGCTGATGCTGCCCTGGAAGATGTGGAGAACTTGCG GCACCTTATCCTGTGGAGCCTGCTGCCTGGTCACACTGTGAAGACTCAGGCTGCTGGCGAGCCTGAAGAT GACCTTACACCCACCCCTTCTGTCGTCAGCATTACCTCTCACCCCTGGGACCCAGGATCCCCAGGGCAAG CTCCTGCTATAAGTGACAACACCCAATTTCCAAGGCCAGAGGGAAGCCAGCCAGAGGGCGAGGATGTTGC TCTCTGTTCTCTGGCACACTTGCCACCAAGGACCAGAAATTCTGGCATCTGGGACTCTCCTGAGCTGGAT AGGAATCCAGCTGAAGAGGCTTCAAGCTCAGAACCAGCAGGAAGTTACAAAGTTGTAAGGAAAGTCTCTC TACTCCCTGGTGGTGGTGTCGGTGCAGCCAAGGTGGCGGGCAGCAATGTTACCCCTGCACTCCCAGAGAG TGGCCAGTCAGAATCTGAGCTATCTGAAGTGGAAGGCGGAGCACAGGCTACGGGGAACTGTTTCTATGTC AGCATGCCAGCAGAACCTCTGGACTCTAGCACTGAGCCTCCTGGGACACCCCCAAGTCTCTCGCAGTGTC ACAGCCTCCCTGCATGGCCAACAGAGCCTCCGCAACACAGGGGAGTCACTGGGGGTCAGCGTTCCAGCCT GGTCCTCAGGGATATGGGTGTGATCTTCCACACCATCGAGCAACTGACCGTCAAGCTTCACAGACTCAAG GACATGGAGCTGGCCCACAGAGAGCTGCTCAACTCCCTTGGAGGAGAGTCGTCTGGTGGAACCACTCCTG TGGGAAGTTTTCACACAGAGGCAGCCAGATGGACAGACTACTCCCTCTCTCCTCCAGCCAAGGAAGCCCT GACCTCTGACCCCCAAAACAATCAGGAGCAGGGGTCCTACCCTGAAGAAGGCTCCGACACCCCCCTGGAA GACAGTGCCACAGACACAGCTTCGTCACCAGGACCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212032 representing BC079565 Red=Cloning site Green=Tags(s) MSIRLPHSIDRLSSLSSLGDSTPERTSPSHHRQPSDTSETTGLVQRCVIIQKDQHGFGFTVSGDRIVLVQ SVRPGGAAMRAGVKEGDRIIKVNGTMVTNSSHLEVVKLIKSGAYVALTLLGSSPSSIGVSGLQQNPSLSG GPRVNPMIPPPPPPPPLPPPQHITGPKPLQDPEVQKHATQILRNMLRQEEKELQRICEVYSRNPASLLEE QIEGARRRVTQLQLKIQQETGGLVDVLPLCGETSQRTCEGRLSVDSQEADSGLDSGTERFPSISESLVNR NSVLSDPGLDSPQTSPVILARVGQHHRRQGSDAAVHPLNHQGIDQSPKPLIIGPEEDYDPGYFNNESDII FQDLEKLKSHPAYLVVFLRYIFSQADPGPLLFYLCSEVYQQTNPKDSRNLGKDIWNIFLEKNAPLRVKIP EMLQAEIDLRLRNSEDPRSALYEAQEAVMPEIQEQINDYRSKRTLGLGSLYGENDLLDLDGDPLRERQMA EKQLAALGDILSKYEEDRSAPMDFAVNTYMSHTGIRLRESRPSSTAEKTQSAPDKDKWLPFFPKTKKSSN SKKEKDALEDKKRNPILRYIGKPKSSSQSIKPGNVRNIIQHFENSHQYDVPEPGTQRLSTGSFPEDLLES DSSRSEIRLGRSESLKGREEMKRSRKAENVPRSRSDVDMDAAAEATRLHQSASSSASSLSTRSLENPTPP FTPKMGRRSIESPNLGFCTDVILPHLLEDDLGQLSDLEPEPEVQNWQHTVGKDVVANLTQREIDRQEVIN ELFVTEASHLRTLRVLDLIFYQRMKKENLMPREELSRLFPNLPELIEIHNSWCEAMKKLREEGPIIRDIS DLMLARFDGPAREELQQVAAQFCSYQSVALELIRTKQRKESRFQLFMQEAESHPQCRRLQLRDLIISEMQ RLTKYPLLLENIIKHTAGGTSEYEKLCRARDQCREILKFVNEAVKQTENRHRLEGYQKRLDATALERASN PLAAEFKSLDLTTRKMIHEGPLTWRISKDKTLDLQVLLLEDLVVLLQRQEEKLLLKCHSKTAVGSSDSKQ TFSPVLKLNAVLIRSVATDKRAFFIICTSELGPPQIYELVALTSSDKNIWMELLEEAVQNATKHPGDAPV LNHPSPPGSQEPAYQGSTSSRVEVNDSEVYPTEREPKKPSEGPGPEQRGQDKQLLAQEGPEQEEDAEELR ALPCPPPSLDGENRGIRTRDPVLLALTGPLLMEGLADAALEDVENLRHLILWSLLPGHTVKTQAAGEPED DLTPTPSVVSITSHPWDPGSPGQAPAISDNTQFPRPEGSQPEGEDVALCSLAHLPPRTRNSGIWDSPELD RNPAEEASSSEPAGSYKVVRKVSLLPGGGVGAAKVAGSNVTPALPESGQSESELSEVEGGAQATGNCFYV SMPAEPLDSSTEPPGTPPSLSQCHSLPAWPTEPPQHRGVTGGQRSSLVLRDMGVIFHTIEQLTVKLHRLK DMELAHRELLNSLGGESSGGTTPVGSFHTEAARWTDYSLSPPAKEALTSDPQNNQEQGSYPEEGSDTPLE DSATDTASSPGP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC079565
ORF Size	4658 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC079565, AAH79565
RefSeq Size	5617 bp
RefSeq ORF	4658 bp
Locus ID	213498

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