Eif2ak4 (BC072637) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	GCN2, MGCN2, 2610011M03
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212025 representing BC072637 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCACCTACATACCCAGATGTGTGATGATATTTGAACTGGCACACCATGTGCAGTCGTTTCTCAGCG AGCATAACAAGCCCCCACCAAAGTCTTTCCATGAAGAAATGCTGGAAAGGCAGGCTCAGGAGAAGCAGCA GAGGTTGCTGGAGGCCAGGCGGAAGGAGGAGCAGGAGCAACGTGAAATCCTCCACGAGATTCAGAGAAGG AAAGAGGAGATCAAGGAAGAGAAGAAAAGGAAGGAAATGGCTAAGCAGGAACGTTTGGAAATCACTAGCT TGACAAACCAGGACTATGCCTCTAAGAGAGACCCAGCAGGGCACAGGGCAGCTGCCATCCTCCATGGAGG CTCTCCTGACTTTGTAGGAAATGGTAAAGCCCGGACATACTCCTCAGGAAGGTCCAGGCGAGAACGTCAG TATTCTGTGTGTAGTGGTGAACCCTCTCCTGGCTCGTGTGACATCCTCCATTTCTCTGTGGGTAGCCCTG ATCAGCTCATGGTGCACAAAGGGAGATGTGTTGGCAGTGATGAGCAGCTGGGAAAGGTGGTATACAATGC TTTGGAAACAGCCACGGGCAGCTTTGTCTTGCTGCATGAGTGGGTCCTTCAATGGCAGAAGATGGGCCCT TGCCTTACCAGTCAAGAGAAAGAGAAGATTGACAAGTGCAAAAGGCAGATTCAAGGAGCAGAAACAGAAT TCAGCTCCCTAGTGAAACTGAGCCATCCAAATATCGTCCGCTACTTTGCGATGAACTCCAGAGAGGAGGA GGACTCCATTGTGATCGACATTCTGGCAGAGCACGTCAGTGGCATCTCTCTGGCTACACACCTGAGCCAC TCGGGCCCAGTCCCCGCCCACCAGCTGCGCAAGTACACAGCCCAGCTCCTGGCAGGCCTGGATTACCTAC ACAGCAACTCCGTGGTGCACAAGGTTCTGAGCGCGTCCAGTGTCTTGGTAGATGCCGAGGGCACTGTCAA GATAACAGACTACAGCATCTCTAAGCGTCTGGCAGACATTTGCAAGGAGGATGTATTTGAGCAAGCTCGA GTTCGTTTTAGTGACAGTGCCCTGCCTTATAAAACAGGAAAGAAAGGGGATGTGTGGCGGCTCGGCCTCC TGCTGTTGTCTCTCAGCCAAGGACAGGAGTGTGGGGAGTATCCGGTGACCATCCCCAGTGACCTGCCAGC TGACTTCCAAGACTTCCTGAAGAAGTGTGTCTGCCTGGACGACAAGGAAAGATGGAGCCCTCAGCAGCTG CTGAAACACAGCTTTATAAACCCTCAACCAAAGCTGCCTTTGGTGGAGCAGAGTCCTGAAGACTCTGGGG GACAAGACTACATTGAGACCGTCATTCCCAGCAACCAGCTGCCCAGCGCTGCGTTCTTCAGTGAGACACA GAAACAGTTCTCCCGGTACTTCATTGAGTTTGAAGAACTACAACTCCTAGGGAAAGGAGCTTTTGGAGCT GTCATCAAGGTGCAAAACAAGCTTGATGGCTGCTGCTATGCTGTGAAGCGCATCCCTATCAACCCTGCAA GCAGACACTTCCGCCGGATCAAGGGCGAGGTGACACTGCTATCGCGCCTGCACCATGAGAACATTGTGCG CTACTACAACGCCTGGATCGAGCGGCATGAGCGCCCAGCGGTGCCAGGGACACCGCCCCCAGACTGCACA CCCCAGGCCCAGGACAGCCCAGCCACCTGCGGGAAAACATCAGGCGACACTGAAGAACTGGGCAGCGTGG AGGCTGCAGCACCGCCACCCATCCTCAGCAGCTCGGTGGAGTGGAGCACATCTGCAGAGCGTTCTACCAG CACCCGCTTCCCAGTCACGGGCCAGGATTCCAGCAGCGATGAGGAGGACGAGGACGAGCGCGATGGCGTC TTCTCCCAGTCCTTTCTACCTGCTTCAGATTCTGACAGTGACATCATCTTTGACAATGAAGATGAAAACA GTAAAAGTCAAAATCAGGATGAAGACTGCAATCAAAAGGACGGTAGCCATGAGATTGAGCCTTCAGTGAC AGCTGAGGCTGTGCACTACCTTTATATCCAGATGGAGTACTGCGAGAAGAGCACGCTGCGGGACACCATT GACCAGGGGCTGTTCCGAGACACCAGCCGGCTCTGGAGGCTTTTCCGAGAGATTCTGGATGGATTAGCTT ATATCCATGAGAAAGGAATGATTCATCGCGACTTGAAGCCTGTCAATATTATTTTGGATTCTGATGACCA TGTGAAAATAGGTGACTTTGGCCTGGCAACAGACCATCTGGCCTTCACTGCTGAAGGTAAACAGGACGAC CAGGCAGGTGACGGAGTGATTAAGTCAGACCCTTCAGGCCATTTGACTGGCATGGTTGGTACTGCTCTGT ATGTAAGCCCTGAGGTCCAAGGAAGCACCAAGTCTGCATACAACCAGAAAGTGGATCTCTTCAGCCTGGG AATTATCTTCTTTGAGATGTCTTATCACCCGATGGTGACTGCCTCAGAAAGAATTTTCGTTCTCAACCAA CTTCGAGATCCCACATCGCCCAAGTTTCCAGACGACTTCGATGATGGAGAACATACAAAGCAGAAATCTG TCATCTCCTGGCTGTTGAACCATGATCCAGCTAAACGGCCCACGGCCATGGAACTGCTCAAGAGTGAGCT GCTCCCCCCGCCGCAGATGGAGGAGTCCGAGCTGCATGAAGTGCTGCACCACACGCTGGCCAACATAGAT GGGAAGGCCTACCGCACCATGATGAGTCAGATCTTCTGCCAGCACATCTCCCCCGCTATAGACTACACCT ACGACAGTGACATCCTGAAGGGCAACTTCTTGATTCGTACAGCCAAGATCCAGCAGCTTGTATGTGAAAC CATCGTCCGCGTCTTTAAAAGGCATGGCGCTGTCCAGCTGTGCACCCCACTGCTGCTTCCCCGAAACAGG CAAATATATGAGCACAACGAAGCGGCTTTGTTCATGGATCACAGCGGCATGCTGGTGATGCTTCCGTTCG ACCTGCGGGTCCCTTTTGCAAGATATGTGGCAAGAAATAACATACTGAATTTAAAACGATACTGCATAGA GCGGGTGTTCAGACCTCGGAAGTTAGACCGATTTCATCCCAAAGAACTCCTGGAATGTGCATTTGATATC GTCACTTCTACCACCAACAGCTCTCTGCCCACTGCCGAAACCATCTACACCATCTATGAAATAATCCAGG AGTTTCCTGCACTTCAGGAAAGGAATTACAGCATTTACTTGAACCATACGATGCTTCTGAAGGCAATACT CCTACACTGTGGGATCCCAGAGGACAAGCTCAGCCAAGTCTACGTCATTCTGTATGATGCTGTGACAGAG AAGCTGACTAGGAGAGAAGTGGAAGCTAAATTCTGTAACCTGTCCTTGTCTTCTAATAGCCTGTGTAGAC TCTACAAGTTCATTGAGCAGAAGGGGGATTTGCAAGACTTAACGCCAACCATCAACTCACTAATAAAACA GAAAACAGGCGTTGCTCAGTTGGTGAAGTATAGCTTAAAAGACCTCGAGGACGTCGTCGGGCTGCTGAAG AAACTTGGCGTGAAGTTACAGGTCTCCATCAACCTGGGCTTGGTCTACAAGGTGCAGCAGCACACTGGCA TCATCTTCCAGTTCCTGGCATTCAGCAAACGCAGGCAAAGGGTTGTGCCTGAGATCCTTGCCGCCGGTGG CAGATACGACCTGCTGATTCCCAAGTTCAGAGGCCCACAGACTGTGGGGCCAGTCCCCACTGCTGTCGGT GTCAGCATAGCCATAGACAAGATATTTGCTGCTGTCCTCAACATGGAAGAGCCTGTTACAGTGAGCTCCT GTGACCTCCTGGTTGTCAGTGTTGGCCAGATGTCCATGTCCAGGGCCATCAACCTAACCCAGAAACTGTG GACAGCGGGCATCACTGCAGAAATCATGTATGACTGGTCCCAGTCCCAGGAAGAGTTACAAGAGTACTGC AGACACCATGAAATCACCTATGTGGCTCTGGTCTCCGATAAAGAAGGAAGCCATGTCAAGGTCAAGTCCT TTGAGAAGGAGAGGCAAACAGAAAAGCGTGTATTGGAATCGGATCTTGTGGATCACGTTATGCAGAAACT AAGAACCAAAGTTGGTGACGAAAGAAATTTCAGAGACGCTTCTGATAATCTTGCCGTACAAACTCTGAAG GGGTCGTTTTCTAATGCTTCAGGTTTGTTTGAAATCCATGGAACTACAGTAGTCCCCAACGTGATTGTTC TAGCACCAGAAAAGCTGTCAGCCAGCACCAGGAGGCGACATGAGATTCAGGTGCAAACCCGACTTCAGAC TACACTTGCCAACTTACATCAGAAAAGCAGTGAAATTGAAATTTTGGCTGTGGACCTACCCAAGGAAACA ATCTTACAGTTTCTATCATTAGAGTGGGATGCTGATGAACAGGCATTTAACACAACTGTGAAGCAGCTGC TGTCACGCCTGCCAAAGCAAAGATACCTCAAACTCGTCTGCGATGAAATTTATAACATCAAAGTTGAAAA GAAGGTGTCAGTGCTGTTCCTGTACAGCTACAGAGATGACTACTACAGAATCCTATTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212025 representing BC072637 Red=Cloning site Green=Tags(s) MPTYIPRCVMIFELAHHVQSFLSEHNKPPPKSFHEEMLERQAQEKQQRLLEARRKEEQEQREILHEIQRR KEEIKEEKKRKEMAKQERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQ YSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGP CLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSH SGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQAR VRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQL LKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGA VIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCT PQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGV FSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTI DQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIILDSDDHVKIGDFGLATDHLAFTAEGKQDD QAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQ LRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANID GKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNR QIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDI VTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTE KLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLK KLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVG VSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYC RHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLK GSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKET ILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC072637
ORF Size	4610 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC072637, AAH72637
RefSeq Size	4951 bp
RefSeq ORF	4610 bp
Locus ID	27103
Gene Summary	Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' in response to low amino acid availability (PubMed:10504407, PubMed:10655230, PubMed:12176355, PubMed:12215525, PubMed:15213227, PubMed:16054071, PubMed:16176978, PubMed:16121183, PubMed:15774759, PubMed:16601681, PubMed:26102367). Plays a role as an activator of the integrated stress response (ISR) required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha/EIF2S1 either to a competitive inhibitor of the translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:10655230, PubMed:11106749, PubMed:12176355, PubMed:15213227, PubMed:16176978, PubMed:26102367). Binds uncharged tRNAs (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:16176978, PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (PubMed:16121183). Plays a role in neurite outgrowth inhibition (PubMed:23447528). Plays a role in feeding behavior to maintain amino acid homeostasis; contributes to the innate aversion toward diets of imbalanced amino acid composition (PubMed:16054071, PubMed:15774759). Plays a proapoptotic role in response to glucose deprivation (PubMed:20660158). Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (PubMed:12176355).[UniProtKB/Swiss-Prot Function]

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