Abca3 (BC079617) Mouse Tagged ORF Clone

CAT#: MG211993

  • TrueORF®

Abca3 (tGFP-tagged) - Mouse ATP-binding cassette, sub-family A (ABC1), member 3 (cDNA clone MGC:90532 IMAGE:30362309)

ORF Plasmid: DDK tGFP



  "BC079617" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 15,870.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Abc3, ABC-C
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211993 representing BC079617
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGTGCTGCGGCAGCTCACCCTCCTACTCTGGAAGAACTATACCCTGAAGAAACGGAAGGTTCTAG
TGACAGTCCTGGAGCTCTTCCTGCCCCTGCTGTTTTCTGGGATCCTGATTTGGCTTCGCTTGAAGATCCA
GTCGGAGAATGTGCCCAATGCCACTGTTTACCCGGACCAGTCCATCCAGGAGCTGCCCCTGTTTTTCTCA
TTCCCTCCACCTGGGGGGACCTGGGAGCTCGCCTATGTCCCTTCCCACAGCGATGCTGCCAGGACCATCA
CGGAGACGGTGAAAAGGGAATTCATGATCAAGATGAGAGTGCACGGCTTCTCCTCTGAGAAGGACTTCGA
GGACTACATTCGCTATGACAACCACTCCTCCAGCGTGCTGGCAGCTGTGGTATTTGAGCACAGCTTCAAC
CACAGCCAGGACCCCCTGCCGCTGGCGGTGAAATACCACCTGCGCTTCAGTTACACACGAAGAAATTACA
TGTGGACCCAAACAGGAAATATTTTCCTAAAAGAGACTGAAGGCTGGCATACCACTTCACTTTTTCCACT
TTTTCCAAGCCCCGGACCGCGGGAACCTTCATCACCTGATGGCGGTGAACCTGGATACATTCGGGAAGGC
TTCCTGGCCATGCAACATGCTGTAGACAAAGCCATCATGCGTTACCATGCCAACACCTCTGCACAGCAGC
TGTTCCAGAAGCTCATGGTGATCACCAAAAGGTTCCCGTTCCCACCATACATCTCAGATCCATTCCTCAT
TGCCATCCAGTACCAGCTTCCTCTGCTGCTCATGCTGAGTTTCACCTACACCTCCCTCACCATCATCCGG
GCTGTGGTGCAGGAGAAGGAGAAAAAACTAAAGGAGTACATGCGCATGATGGGGCTTAACAGCTGGCTAC
ACTGGAGCGCTTGGTTCCTCATGTTCTTCCTATTCTTCCTCATCGTGGTCTCCTTCATGACCCTCCTGTT
CTGTGTCAAAGTGAAGAAGGACATAGCAGTCCTTTCCAACAGCGACCCCTCCCTGGTCCTGGCGTTCCTG
CTGTGTTTTGCAATCTCCTCCATTTCCTTCAGCTTCATGGTCAGCACCTTCTTCAGTAAAGCCAACATAG
CAGCAGCCGTGGGTGGCTTCCTGTACTTCTTCACCTATACCCCCTACTTTTTCGTGGCTCCTCGGTACAA
CTGGATGACACTGAGTCAAAAGCTCTTGTCCTGTCTCCTATCTAATGTTGCCATGGCAATGGGAGCCCAG
CTCATAGGAAAATTCGAAGCCAAAGGCACTGGCATCCAGTGGCGGGACCTCCTCAACCCTGTCAACGTGG
ACGACAATTTCTGCTTTGGACAAGTGCTGGGGATGCTGCTGCTGGACTCAGCACTCTATGGCCTGGTGAC
CTGGTACGTGGAGGCTGTCTTCCCAGGCCAGTTTGGCGTACCCCAGCCCTGGCACTTCTTCCTTATGCCC
TCCTACTGGTGTGGGAACCCAAGAACTGTCGTAGGGAAGGAAGAGGAAGGCAGCGACCCAGAGAAAGCTC
TCAGGAACGAGTACTTTGAAGCCGAGCCAGAGGACTTGGTAGCTGGAATCAAGATCAAGCATCTCTCCAA
GGTGTTCCAAGTGGGCAATAAGGACAAGATGGGCATCAGAGACCTGACCCTGAACCTCTACGAAGGACAG
ATAACAGTCCTCCTGGGCCACAATGGTGCTGGGAAGACCACGACCATGTCTCTGCTGACAGGCCTCTTCC
CCCCTACCAGTGGACATGCTTATATTCATGGGTACGAAATTTCCCAAGACATGGCTCAGATCCGCAAGAG
TTTGGGCCTGTGTCCCCAGCATGATGTACTGTTTGACAACCTGACAGTTGCCGAACATCTTTACTTCTAT
GCACAGCTGAAAGGCCTCTCTCTCCAGAAGTGTCCTGAGGAAGTCAAGCAGATGCTACACATCCTCAGCC
TGGAGGACAAGCGTGACTTACGGTCCAAGTTCCTGAGTGGGGGCATGAAGCGCAAGCTCTCCATTGGCAT
CGCCCTCATAGCGGGCTCTAAGGTGCTGATGTTAGATGAGCCCACCTCAGGCATGGATGCAGTGTCCAGA
AGGGCCATCTGGGACCTTCTCCAGCAGCAGAAGAGTGACCGCACCGTCCTGCTGACCACTCACTTCATGG
ACGAAGCTGACCTGCTGGGAGACCGCATCGCCATCTTGGCCAAGGGGGAGCTCCAGTGCTGCGGGTCTTC
ACTATTTCTCAAGCAGAAATACGGAGCTGGCTACCACATGACGCTGGTAAAAGAACCGCACTGCAATCCT
GAAGGAATCTCTCAGCTGGTGCACCACCATGTCCCCAATGCCATGCTGGAGAGCCATGCTGGGGCTGAGC
TGTCCTTTATTCTTCCCAAGGAGAGCACGCACAGGTTTGAAAGTCTGTTTGCTAAACTGGAAAAGAAGCA
GAAGGAGTTGGGCATCGCAAGCTTCGGGGCCTCGGTCACCACCATGGAGGAAGTCTTCCTTCGGGGCCGG
AAGGGATTTGACATTGCCCTCAATTTGCTCATCGCCATGGCATTTCTGGCCAGCACCTTTTCCATCCTGG
CTGTCAGTGAGAGGGCCGTCCAGGCCAAGCACGTTCAGTTTGTGAGCGGTGTCCATGTGGCTACTTTCTG
GTTCTCCGCTCTGTTGTGGGACCTCATCTCCTTCCTCGTTCCCAGTCTACTACTTCTGGTGGTGTTCCAA
GCCTTCAATGTGCACGCCTTCACGAGGGATGGTCACATGGCTGACCTGCTGCTGCTGCTCATGCTCTACG
GCTGGGCCATCATCCCCCTCATGTACCTCATGAGCTTCTTCTTCTCGGCTGCGTCCACAGCTTATACCAG
GCTGACCATATTCAACATCCTGTCGGGCATCGCTACCTTCATCATGGTCACTATCATGCGTATCCCAGCT
GTGAAGTTAGAAGAACTTTCCAGAACCTTAGATCATGTGTTCTTGGTGCTGCCAAACCACTGCCTGGGGA
TGGCGGTCAGCAACTTCTATGAGAACTATGAGACTCGGCGATACTGCACTTCCTCAGAGCTTGCTGCCCA
CTACTGCAAGAAGTACAACATCCAGTACCAGGAGAGCTTCTATGCCTGGAGCACCCCAGGCGTGGGCAAG
TTTGTGACTTCCATGGCTGCCTCAGGGGGCATCTACCTCACCCTGCTGTTCCTCATTGAGACCAACCTGC
TGTGGCGACTGAGAACCTTCATCTGTGCCTTCCGGAGGAGGTGGACTCTGGCAGAACTGCAGAACCGGAC
ATCAGTGCTGCCCGAGGACCAGGATGTAGCTGAGGAGAGGAGCCGAATCCTGGTCCCTAGCTTGGACTCC
ATGCTCGACACACCACTGATCATCAACGAGCTCTCCAAGGTGTATGACCAGCGAGCACCGCTCCTTGCCG
TGGACAGGATCTCCCTTGCGGTCCAGAAAGGGGAGTGCTTCGGCCTGTTGGGTTTCAATGGAGCTGGAAA
AACCACAACATTCAAAATGCTGACTGGGGAGGAGACCATCACCTCAGGGGATGCCTTTGTTGGTGGTTAC
AGCATCAGTTCTGACATCGGGAAGGTGCGGCAGCGGATGGGCTACTGCCCCCAGTTTGATGCACTGCTTG
ATCACATGACTGGCAGGGAGATGCTGGTTATGTATGCACGGCTCCGAGGCATCCCAGAGCGGCTCATCAA
TGCCTGTGTGGAGAATACTCTGCGGGGTCTGCTGCTGGAACCGCACGCCAACAAACTAGTCAAGACTTAC
AGTGGTGGTAACAAACGCAAGCTAAGCACTGGCATTGCCCTCATTGGAGAGCCTGCAGTCATCTTCCTGG
ATGAGCCATCGACCGGCATGGACCCAGTGGCCCGGCGTCTACTCTGGGATACTGTAGCCCGGGCCCGAGA
GTCTGGCAAAGCCATTGTCATCACCTCCCACAGTATGGAGGAGTGTGAAGCCTTATGTACCAGGCTGGCC
ATCATGGTACAGGGCCAGTTCAAGTGCCTGGGCAGCCCACAGCACCTCAAGAGCAAGTTTGGCAGCGGCT
ACTCCCTGCAGGCCAAGGTCCGGAGCGAGGGGAAGCAAGACGCGCTAGAGGAGTTCAAGGCCTTTGTGGA
CCTGACCTTCCCAGGCAGCATCCTAGAAGATGAGCACCAAGACATGGTCCATTATCATCTGCCTGGCTGT
GACCTCAGCTGGGCCAAGGTATTTGGTATCCTGGAGAAAGCCAAGGAGAAGTATGGAGTGGATGATTACT
CTGTGAGCCAGATCTCCCTGGAGCAGGTCTTTCTGAGCTTTGCCCACCTACAACCCCCCACCACTGAGGA
TGGCCGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211993 representing BC079617
Red=Cloning site Green=Tags(s)

MAVLRQLTLLLWKNYTLKKRKVLVTVLELFLPLLFSGILIWLRLKIQSENVPNATVYPDQSIQELPLFFS
FPPPGGTWELAYVPSHSDAARTITETVKREFMIKMRVHGFSSEKDFEDYIRYDNHSSSVLAAVVFEHSFN
HSQDPLPLAVKYHLRFSYTRRNYMWTQTGNIFLKETEGWHTTSLFPLFPSPGPREPSSPDGGEPGYIREG
FLAMQHAVDKAIMRYHANTSAQQLFQKLMVITKRFPFPPYISDPFLIAIQYQLPLLLMLSFTYTSLTIIR
AVVQEKEKKLKEYMRMMGLNSWLHWSAWFLMFFLFFLIVVSFMTLLFCVKVKKDIAVLSNSDPSLVLAFL
LCFAISSISFSFMVSTFFSKANIAAAVGGFLYFFTYTPYFFVAPRYNWMTLSQKLLSCLLSNVAMAMGAQ
LIGKFEAKGTGIQWRDLLNPVNVDDNFCFGQVLGMLLLDSALYGLVTWYVEAVFPGQFGVPQPWHFFLMP
SYWCGNPRTVVGKEEEGSDPEKALRNEYFEAEPEDLVAGIKIKHLSKVFQVGNKDKMGIRDLTLNLYEGQ
ITVLLGHNGAGKTTTMSLLTGLFPPTSGHAYIHGYEISQDMAQIRKSLGLCPQHDVLFDNLTVAEHLYFY
AQLKGLSLQKCPEEVKQMLHILSLEDKRDLRSKFLSGGMKRKLSIGIALIAGSKVLMLDEPTSGMDAVSR
RAIWDLLQQQKSDRTVLLTTHFMDEADLLGDRIAILAKGELQCCGSSLFLKQKYGAGYHMTLVKEPHCNP
EGISQLVHHHVPNAMLESHAGAELSFILPKESTHRFESLFAKLEKKQKELGIASFGASVTTMEEVFLRGR
KGFDIALNLLIAMAFLASTFSILAVSERAVQAKHVQFVSGVHVATFWFSALLWDLISFLVPSLLLLVVFQ
AFNVHAFTRDGHMADLLLLLMLYGWAIIPLMYLMSFFFSAASTAYTRLTIFNILSGIATFIMVTIMRIPA
VKLEELSRTLDHVFLVLPNHCLGMAVSNFYENYETRRYCTSSELAAHYCKKYNIQYQESFYAWSTPGVGK
FVTSMAASGGIYLTLLFLIETNLLWRLRTFICAFRRRWTLAELQNRTSVLPEDQDVAEERSRILVPSLDS
MLDTPLIINELSKVYDQRAPLLAVDRISLAVQKGECFGLLGFNGAGKTTTFKMLTGEETITSGDAFVGGY
SISSDIGKVRQRMGYCPQFDALLDHMTGREMLVMYARLRGIPERLINACVENTLRGLLLEPHANKLVKTY
SGGNKRKLSTGIALIGEPAVIFLDEPSTGMDPVARRLLWDTVARARESGKAIVITSHSMEECEALCTRLA
IMVQGQFKCLGSPQHLKSKFGSGYSLQAKVRSEGKQDALEEFKAFVDLTFPGSILEDEHQDMVHYHLPGC
DLSWAKVFGILEKAKEKYGVDDYSVSQISLEQVFLSFAHLQPPTTEDGR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC079617
ORF Size 4347 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC079617.1
RefSeq Size 5228 bp
RefSeq ORF 4349 bp
Locus ID 27410
Gene Summary Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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