Cpsf1 (BC056388) Mouse Tagged ORF Clone
CAT#: MG211989
- TrueORF®
Cpsf1 (tGFP-tagged) - Mouse cleavage and polyadenylation specific factor 1 (cDNA clone MGC:73492 IMAGE:6854851)
Need custom modification / cloning service?
Get a free quote
CNY 11,880.00
货期*
5周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG211989 representing BC056388
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGCGGTGTACAAGCAGGCGCACCCGCCCACCGGGCTGGAGTTTACCATGTACTGCAACTTCTTCA ACAACAGTGAGCGCAACCTCGTGGTGGCCGGCACCTCGCAGCTCTATGTGTACCGCCTGAACCGCGACGC CGAGGCTCTGACCAAGAATGATGGGAGCACAGAGGGGAAGGCCCACCGAGAGAAACTAGAGCTGGTAGCC TCCTTCTCTTTTTTTGGCAATGTCATGTCCATGGCCAGTGTGCAGCTTGCAGGCGCCAAACGTGATGCCT TGCTTCTCAGTTTCAAAGACGCTAAGCTGTCTGTGGTGGAGTATGACCCAGGCACTCATGACCTGAAGAC CCTGTCTCTACACTACTTTGAGGAGCCTGAACTTCGGGATGGGTTTGTGCAGAACGTGCATACGCCTCGT GTGCGGGTGGACCCTGACGGGCGCTGTGCAGCAATGCTCATCTATGGCACGAGGCTGGTAGTTCTGCCTT TCCGCAGAGAGAGCCTGGCTGAGGAACATGAGGGGCTCATGGGCGAAGGGCAGAGGTCTAGCTTCTTGCC CAGCTACATCATTGATGTACGGGCTCTGGATGAGAAACTACTCAACATCATTGACCTGCAGTTCCTGCAT GGCTACTATGAGCCCACCCTGCTTATCCTGTTTGAGCCCAACCAGACTTGGCCAGGGCGGGTGGCTGTGA GGCAGGACACGTGCTCCATTGTGGCTATCTCGCTGAACATCACACAGAAAGTCCATCCAGTCATCTGGTC CCTCACCAGCTTGCCTTTTGACTGTACCCAGGCCCTGGCTGTGCCCAAGCCCATAGGTGGGGTAGTGATC TTCGCTGTCAACTCACTGTTGTACCTGAACCAGAGTGTTCCCCCATATGGTGTGGCTCTCAATAGTCTCA CCACAGGCACCACCGCTTTCCCATTGCGTACCCAAGAGGGTGTACGGATCACCCTAGACTGCGCACAGGC GGCCTTCATCTCTTATGACAAGATGGTCATCTCCCTCAAGGGTGGTGAGATTTATGTGCTGACCCTCATC ACTGACGGCATGCGAAGTGTCCGAGCGTTTCACTTTGACAAGGCAGCTGCTAGTGTCCTTACCACCAGTA TGGTCACAATGGAGCCCGGATACCTGTTCCTAGGCTCTCGCCTGGGCAATTCCCTCCTCCTCAAGTACAC GGAGAAGCTGCAGGAGCCCCCAGCCAGCTCCGTCCGTGAGGCTGCTGACAAGGAAGAGCCACCCTCAAAG AAGAAGCGAGTGGAGCCTGCAGTGGGCTGGACAGGAGGCAAGACGGTGCCACAGGACGAGGTGGATGAAA TTGAAGTTTATGGCAGTGAGGCCCAGTCAGGCACACAGCTGGCTACTTACTCTTTTGAGGTATGTGACAG CATGCTCAACATTGGACCCTGTGCCAACGCTGCTGTGGGCGAGCCTGCCTTCCTCTCTGAAGAGTTCCAG AATAGCCCTGAGCCAGACCTGGAGATTGTGGTTTGTTCCGGCTACGGGAAGAATGGGGCCCTGTCAGTGT TGCAGAAGAGTATCCGGCCCCAGGTGGTGACAACCTTTGAACTTCCTGGCTGCTATGATATGTGGACAGT CATTGCTCCTGTGCGGAAAGAGGAGGAAGAAACCCCCAAAGCAGAGAGCACAGAGCAGGAGCCCAGTGCC CCGAAAGCAGAGGAGGATGGGCGAAGACATGGCTTCCTTATTCTTAGCCGGGAAGACTCAACCATGATCC TACAGACTGGCCAGGAGATCATGGAGCTGGACACCAGTGGTTTTGCCACTCAGGGTCCTACTGTCTTTGC TGGAAACATTGGGGACAATCGTTACATTGTTCAAGTCTCGCCACTGGGCATTCGCCTGTTGGAAGGCGTG AATCAATTGCACTTCATCCCTGTGGACCTGGGCGCCCCCATCGTGCAGTGTGCCGTGGCTGATCCCTATG TGGTTATCATGAGTGCTGAGGGACACGTTACCATGTTCCTACTCAAGAGCGATTCCTATGGTGGCCGTCA TCACCGCCTGGCACTACACAAGCCTCCACTGCATCATCAGTCCAAGGTGATTGCTCTCTGCCTGTACCGC GATGTCAGTGGCATGTTCACCACTGAGAGCCGCCTGGGTGGGGCCCGTGATGAACTGGGTGGCCGAAGTG GCTCCGAGGCAGAAGGCCTGGGCTCAGAAACGAGCCCCACAGTGGATGATGAGGAAGAGATGCTCTATGG GGACTCCAGTGCCCTCTTCAGTCCCAGCAAAGAGGAGGCCCGTAGGAGCAGCCAGCCACCGGCTGATCGT GACCCTGCACCCTTCAAGGCAGACCCCACTCACTGGTGCCTGCTGGTGCGGGAGAATGGCACCATGGAGA TCTACCAGCTCCCAGACTGGCGGCTGGTATTCCTAGTTAAGAACTTCCCCGTGGGGCAGCGGGTTCTGGT GGACAGTTCATTTGGACAGCCCACTACACAAGGTGAAGTCCGAAAGGAAGAAGCCACACGCCAGGGGGAG CTGCCTTTGGTTAAGGAGGTGTTGTTGGTTGCTCTGGGAAGCCGGCAGAGCAGGCCCTACCTGCTGGTAC ACGTGGACCAGGAGTTGCTTATCTATGAAGCCTTTCCCCATGACTCTCAGCTTGGCCAGGGAAATCTTAA AGTCCGTTTCAAAAAGGTCCCTCACAATATCAACTTCCGTGAAAAGAAGCCAAAGCCGTCCAAGAAGAAG GCCGAAGGTTGCAGCACTGAGGAGGGATCTGGAGGCCGGGGCCGTGTGGCACGTTTCCGCTACTTTGAGG ATATTTATGGCTATTCGGGGGTTTTCATCTGTGGACCCTCACCTCACTGGCTCCTGGTGACTGGCCGCGG AGCTCTGCGTCTGCACCCCATGGGCATTGATGGCCCCATTGACTCCTTTGCTCCTTTCCACAATGTGAAC TGCCCCCGTGGTTTCCTGTACTTCAACAGACAGGGGGAGCTGAGGATCAGCGTCCTGCCTGCCTACCTGT CCTACGATGCCCCATGGCCTGTCAGGAAGATCCCACTCCGATGCACAGCCCACTATGTGGCCTACCATGT GGAATCCAAGGTCTATGCTGTGGCCACTAGTACCAACACACCTTGCACTAGGATCCCACGCATGACTGGC GAGGAAAAAGAATTCGAGGCCATTGAGAGAGACGACAGATACATCCACCCCCAGCAAGAAGCCTTCTCCA TCCAGCTCATCTCCCCGGTCAGCTGGGAGGCCATTCCCAATGCCAGGATCGAGCTAGAGGAATGGGAGCA TGTCACGTGCATGAAGACAGTGTCACTGCGCAGTGAGGAGACCGTGTCAGGCCTAAAGGGCTATGTGGCT GCTGGGACCTGCCTCATGCAGGGGGAGGAGGTCACGTGCCGTGGGCGGATCCTGATCATGGATGTGATTG AAGTGGTGCCTGAGCCTGGGCAGCCCCTGACCAAGAACAAATTCAAGGTCCTGTATGAGAAGGAGCAGAA AGGGCCCGTGACTGCGCTGTGCCACTGCAATGGCCACCTGGTGTCGGCCATTGGCCAAAAAATCTTCCTG TGGAGCCTGCGGGCTAGCGAGCTGACCGGCATGGCCTTCATCGACACCCAGCTGTACATCCACCAGATGA TCAGTGTCAAGAACTTCATCCTGGCAGCAGACGTCATGAAGAGCATCTCACTGCTGCGTTACCAAGAGGA GAGCAAGACGCTGAGCCTGGTGTCCCGGGATGCCAAGCCTTTGGAGGTATACAGCGTGGACTTCATGGTG GACAATGCCCAACTGGGCTTCTTGGTGTCTGACCGAGACCGCAATCTTATGGTGTATATGTATCTGCCAG AAGCCAAAGAGAGTTTTGGGGGCATGCGCCTGCTACGCAGAGCGGATTTCCATGTGGGTGCCCACGTGAA TACGTTCTGGAGGACCCCGTGCCGAGGTGCTGCCGAGGGCCCCAGCAAGAAGTCAGTCGTGTGGGAAAAC AAGCATATCACATGGTTTGCAACCCTTGACGGTGGCATTGGGCTCCTGCTGCCCATGCAAGAAAAGACAT ACCGACGCTTGCTGATGCTGCAGAATGCACTGACGACCATGCTACCCCACCACGCTGGCCTCAACCCCCG CGCCTTCCGAATGTTGCACGTAGACCGGCGCATCTTGCAGAATGCTGTGCGCAACGTCCTTGATGGGGAA CTGCTAAACCGCTACCTATACCTCAGCACCATGGAGCGCAGTGAGCTGGCCAAGAAGATAGGTACCACGC CTGACATTATCCTGGATGACTTGCTGGAGACAGACCGGGTCACGGCTCACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211989 representing BC056388
Red=Cloning site Green=Tags(s) MYAVYKQAHPPTGLEFTMYCNFFNNSERNLVVAGTSQLYVYRLNRDAEALTKNDGSTEGKAHREKLELVA SFSFFGNVMSMASVQLAGAKRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPELRDGFVQNVHTPR VRVDPDGRCAAMLIYGTRLVVLPFRRESLAEEHEGLMGEGQRSSFLPSYIIDVRALDEKLLNIIDLQFLH GYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNITQKVHPVIWSLTSLPFDCTQALAVPKPIGGVVI FAVNSLLYLNQSVPPYGVALNSLTTGTTAFPLRTQEGVRITLDCAQAAFISYDKMVISLKGGEIYVLTLI TDGMRSVRAFHFDKAAASVLTTSMVTMEPGYLFLGSRLGNSLLLKYTEKLQEPPASSVREAADKEEPPSK KKRVEPAVGWTGGKTVPQDEVDEIEVYGSEAQSGTQLATYSFEVCDSMLNIGPCANAAVGEPAFLSEEFQ NSPEPDLEIVVCSGYGKNGALSVLQKSIRPQVVTTFELPGCYDMWTVIAPVRKEEEETPKAESTEQEPSA PKAEEDGRRHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVFAGNIGDNRYIVQVSPLGIRLLEGV NQLHFIPVDLGAPIVQCAVADPYVVIMSAEGHVTMFLLKSDSYGGRHHRLALHKPPLHHQSKVIALCLYR DVSGMFTTESRLGGARDELGGRSGSEAEGLGSETSPTVDDEEEMLYGDSSALFSPSKEEARRSSQPPADR DPAPFKADPTHWCLLVRENGTMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFGQPTTQGEVRKEEATRQGE LPLVKEVLLVALGSRQSRPYLLVHVDQELLIYEAFPHDSQLGQGNLKVRFKKVPHNINFREKKPKPSKKK AEGCSTEEGSGGRGRVARFRYFEDIYGYSGVFICGPSPHWLLVTGRGALRLHPMGIDGPIDSFAPFHNVN CPRGFLYFNRQGELRISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVESKVYAVATSTNTPCTRIPRMTG EEKEFEAIERDDRYIHPQQEAFSIQLISPVSWEAIPNARIELEEWEHVTCMKTVSLRSEETVSGLKGYVA AGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGHLVSAIGQKIFL WSLRASELTGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDFMV DNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGAAEGPSKKSVVWEN KHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRILQNAVRNVLDGE LLNRYLYLSTMERSELAKKIGTTPDIILDDLLETDRVTAHF TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | BC056388 |
ORF Size | 4325 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC056388, AAH56388 |
RefSeq Size | 4538 bp |
RefSeq ORF | 4325 bp |
Locus ID | 94230 |
Gene Summary | Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
You may also need
Customer
Reviews
Loading...