Synj2 (BC058749) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI481647; mKIAA0348; SJ2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211986 representing BC058749 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTAAGCAAAGGGCTGCGGCTGCTGGCGCGGCTGGACCCCACTGGCCCGAGCAGCGTGCTGTTGG AGGCGCGGGGCCGCGGCGACTGCCTGCTCTTCGAGGCCGGCGCAGTGGCCACGCTGGCTCCGGAAGAAAA GGAAGTCATTAAAGGACTGTATAGCAAGCTGACGGATGCCTATGGCTGCCTCGGGGAGCTGAGGTTACAA TCCGGTGGCGTCCCCTTGAGCTTTCTGGTGTTGGTGACAGGCTGCATGTCAGTGGGCAGAATTCCAGATG CAGAGATCTACAAAATCACTGCCACTGAGTTGTACCCCCTGCAGGAAGAGGCCAAGGAAGAGGACCGCCT GCCCACCTTAAAGAAAATCCTGAGCTCAGGGGTGTTCTATTTCGCATGGCCCAATGATGGCGCCTGCTTC GATCTGACCATCAGGGCTCAGAAACAGGGTGATGACGGCTCTGAATGGGGGACCTCTTTCTTCTGGAACC AGCTATTGCATGTGCCTCTGCGGCAGCACCAGGTGAACTGTCATAACTGGTTGCTGAAAGTCATCTGTGG GGTGGTGACCATCCGCACAGTATATGCCTCCCACAAGCAGGCCAAGGCCTGTCTCATCTCTCGCATCAGC TGTGAACGCGCAGGTGCTCGCTTCCTCACCCGTGGTGTGAACGATGATGGCCACGTGTCCAACTTTGTGG AGACAGAGCAGACGATTTACATGGATGATGGAGTATCGTCCTTTGTCCAGATCCGAGGCTCCGTTCCGCT GTTCTGGGAGCAACCAGGACTTCAGGTTGGCTCCCATCATCTGAGACTGCACAGAGGCCTAGAGGCCAAC GCTCCTGCTTTTGAAAGGCACATGGTGCTTCTGAAGGAGCAATACGGTAAGCAGGTGGTTGTGAACCTGC TGGGTAGCAGAGGCGGTGAAGAGGTGCTCAACAGAGCCTTCAAGAAGTTGCTCTGGGCTTCTTGCCACGC GGGTGACACACCTATGATAAATTTTGACTTCCATCAGTTTGCCAAAGGTAGGAAGCTAGAGAAATTGGAG AACCTGTTGAGACCTCAGTTACAGCTACACTGGGAAGACTTCGGCGTGTTTGCGAAGGGCGAGAATGTAA GTCCACGGTTTCAGAAAGGCACTCTGCGGATGAACTGTCTCGACTGTCTGGATAGAACCAACACTGTGCA GTGCTTCATTGCTCTTGAGGTCCTTCATCTGCAGCTTGAGAGCTTGGGGCTAAATTCAAAGCCCATCATT GACCGTTTTGTGGAGTCCTTCAAAGCCATGTGGTCTCTGAATGGGCACAGCCTGAGCAAGGTGTTCACAG GGAGCAGGGCCCTGGAAGGAAAGGCCAAGGTGGGAAAGCTGAAGGATGGGGCCCGATCCATGTCTCGCAC CATCCAGTCCAACTTCTTCGACGGGGTGAAGCAGGAGGCCATCAAGCTACTGCTAGTCGGAGATGTCTAC AATGAAGAGTCTACAGACAAAGGACGGATGCTGCTGGACAACACGGCCCTTCTGGCGACCCCCAGGATCT TGAAGGCCATGACAGAACGCCAGTCGGAATTCACGAATTTCAAGCGCATCCAGATTGCTGTGGGGACCTG GAATGTGAACGGAGGAAAGCAATTCCGTAGCAATCTCCTGGGGACGGCTGAGCTCACGGACTGGCTCCTA GATGCTCCTCAGCTGTCAGGAGCAGTGGACTCCCAGGATGATGGCAGTCCTGCTGACGTATTTGCCATCG GGTTTGAGGAGATGGTGGAACTGAGTGCGGGAAATATTGTCAATGCCAGCACCACCAACAGGAAGATGTG GGGCGAGCAGCTTCAGAAAGCCATCTCCCGCTCCCATCGGTACATCCTCTTGACCTCCGCACAGCTGGTG GGCGTCTGTCTTTACATCTTTGTACGTCCGTACCACGTCCCGTTCATCAGAGACGTGGCCATCGACACCG TGAAGACCGGCATGGGGGGAAAGGCGGGGAATAAGGGTGCTGTGGGCATCCGCTTCCAGCTCCACAGCAC CAGTTTCTGCTTCGTCTGTAGCCACCTGACGGCTGGGCAGTCTCAGGTGAAGGAGAGGAATGAAGACTAC CGGGAGATCACGCACAAACTCTCCTTCCCTTCGGGGAGAAACATATTTTCACATGATTACGTGTTTTGGT GTGGCGATTTCAACTACCGTATTGATCTTACTTACGAAGAAGTCTTCTATTTTGTTAAACGCCAAGACTG GAAGAAACTTATGGAATTCGATCAGTTACAGTTACAGAAATCAAGTGGAAAAATTTTTAAAGACTTTCAT GAAGGAGCCGTTAACTTCGGACCCACCTACAAGTATGACGTTGGATCAGCTGCCTACGACACAAGTGACA AGTGCCGTACCCCAGCCTGGACAGACAGGGTGCTGTGGTGGAGGAAGAAGCATCCATATGATAAAACAGC TGGTGAACTCAACCTTCTAGACAGCGATCTAGACGGCGATCCCCAAATCAGACACACCTGGTCTCCAGGC ACTCTGAAATACTACGGCCGTGCAGAGCTGCAGGCGTCTGATCACAGACCTGTGCTGGCCATTGTGGAGG TGGAGGTTCAAGAGGTGGATGTAGGAGCCCGGGAGAGGGTCTTCCAGGAAGTGTCCTCTGTCCAAGGCCC GCTGGATGCCACCGTTGTTGTAAACCTCCAGTCTCCAACCCTAGAAGAGAAAAATGAATTTCCAGAGGAC CTGCGCACAGAACTTATGCAGACTTTGGGGAATTATGGGACGATTATTCTAGTCAGGATCAACCAAGGGC AGATGCTGGTGACGTTTGCAGACAGCCACTCGGCTCTCAGTGTGCTGGATGTGGATGGTATGAAGGTGAA AGGCAGGGCCGTGAAGATTCGACCAAAGACCAAAGATTGGCTAGAAGGCCTGAGAGAGGAGCTTCTCCGG AAGCGAGACAGCATGGCCCCTGTGTCTCCCACCGCCAACTCCTGCTTGTTGGAGGAGAACTTTGACTTCT CGAGTCTGGACTATGAGTCCGAAGGGGATGTTCTTGAAGAGGATGAAGACTATTTAGTGGATGGGTTTGG CCAGCCTGTAGTCTCAGACAGTGAGCTCGGTGGAGACAACTCTTCCGACACCATGAGCTCCTTGACACCC GCCAGCAAGTCTCCCGCCCTGGCTAAAAAGAAGCAACATCCAACATACAAAGCTGGCTTAATGGTGAAGA AGTCAGCCTCAGACGCGTCCATCTCTTCTGGCACTCATGGACAATATTCCATCTTGCAGACAGCGAAACT TCTCCCAGGAGCACCCCAGCAACCACCCAAGGCTAGAACTGGAATAAGTAAACCTTACAACGTCAAACAG ATCAAAACCACCAACGCTCAGGAGGCAGAAGCAGCTATCCGGTGTCTCCTGGAAGCTAGCGGAGGGGTCC CGGAATCAGCCCCAGGTGCCATACCCCTGAGAAACCAAGGGTCTTCTAAGCCAGAGGCCACCCTGGGGCC CCCAGCCCTGCCCCGCCGGCCTGCTCCAAGGGTTCCCACTATGAAGAAACCAACTTTGAGGAGGACAGGA AAGCCCATGTTACCAGAAGAAAACTTTGAGCCACAGCCTGTCCATTTTACAATGGCTTCCCAGGAAATGA ACCTTGAGACCCCTCCTCCAATAACAGCTACTCCCATCCCGCCTGTTCCCAAACCAAGAACACTTCAGCC TGGGAAAGGTGTTGAGGGGAGGCCAAGCAGCGGTAAGCCAGAACCAGATGAAGCTCCTTCCGTGACAGGC ACTGTGGAGTCACCACCTCCAGAGGCCCAGGAGGCCCCGTCTCTGGCTCCCAAGGTGCCTCCGAGGAGGA AGAAGTCTGCACCAGCGGCCTTCCACCTGCAGGTCCTGCAGAACAACAGCCAAGTTCTCCAGGGCCTCAC GTGCTCCAGCAGTTCTCCACCCTCACTGAAGCCCGACACCCACCCGCTCTGTCTACAAGTGGCACTTGGC ACTTCATCTGCCAGAAGCCCTGAAACCCATGGCCCCAGAGTGACAGAGCCCGAGGCGGCCTCTTTTCATG GCAACTATCCAGATCCCTTCTGGAGCCTTCTCCACCACCCTAAGCTGTTGAACAATACCTGGCTGTCCAA GAGCTCCGAGCCTTTAGACGTGGGGTCCAGGAACCCTGAGAGGACACACACAGAGCCGGCACAGGTCAAT GCATCGTTGGCTGAGAGGGGGCTTCCACCAGACCATGGGGGAAAAGACTTAAGTCACTGGGTGACAGCTA GTAACAAAGACAAGAGGACAACATTAGGTGTT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211986 representing BC058749 Red=Cloning site Green=Tags(s) MALSKGLRLLARLDPTGPSSVLLEARGRGDCLLFEAGAVATLAPEEKEVIKGLYSKLTDAYGCLGELRLQ SGGVPLSFLVLVTGCMSVGRIPDAEIYKITATELYPLQEEAKEEDRLPTLKKILSSGVFYFAWPNDGACF DLTIRAQKQGDDGSEWGTSFFWNQLLHVPLRQHQVNCHNWLLKVICGVVTIRTVYASHKQAKACLISRIS CERAGARFLTRGVNDDGHVSNFVETEQTIYMDDGVSSFVQIRGSVPLFWEQPGLQVGSHHLRLHRGLEAN APAFERHMVLLKEQYGKQVVVNLLGSRGGEEVLNRAFKKLLWASCHAGDTPMINFDFHQFAKGRKLEKLE NLLRPQLQLHWEDFGVFAKGENVSPRFQKGTLRMNCLDCLDRTNTVQCFIALEVLHLQLESLGLNSKPII DRFVESFKAMWSLNGHSLSKVFTGSRALEGKAKVGKLKDGARSMSRTIQSNFFDGVKQEAIKLLLVGDVY NEESTDKGRMLLDNTALLATPRILKAMTERQSEFTNFKRIQIAVGTWNVNGGKQFRSNLLGTAELTDWLL DAPQLSGAVDSQDDGSPADVFAIGFEEMVELSAGNIVNASTTNRKMWGEQLQKAISRSHRYILLTSAQLV GVCLYIFVRPYHVPFIRDVAIDTVKTGMGGKAGNKGAVGIRFQLHSTSFCFVCSHLTAGQSQVKERNEDY REITHKLSFPSGRNIFSHDYVFWCGDFNYRIDLTYEEVFYFVKRQDWKKLMEFDQLQLQKSSGKIFKDFH EGAVNFGPTYKYDVGSAAYDTSDKCRTPAWTDRVLWWRKKHPYDKTAGELNLLDSDLDGDPQIRHTWSPG TLKYYGRAELQASDHRPVLAIVEVEVQEVDVGARERVFQEVSSVQGPLDATVVVNLQSPTLEEKNEFPED LRTELMQTLGNYGTIILVRINQGQMLVTFADSHSALSVLDVDGMKVKGRAVKIRPKTKDWLEGLREELLR KRDSMAPVSPTANSCLLEENFDFSSLDYESEGDVLEEDEDYLVDGFGQPVVSDSELGGDNSSDTMSSLTP ASKSPALAKKKQHPTYKAGLMVKKSASDASISSGTHGQYSILQTAKLLPGAPQQPPKARTGISKPYNVKQ IKTTNAQEAEAAIRCLLEASGGVPESAPGAIPLRNQGSSKPEATLGPPALPRRPAPRVPTMKKPTLRRTG KPMLPEENFEPQPVHFTMASQEMNLETPPPITATPIPPVPKPRTLQPGKGVEGRPSSGKPEPDEAPSVTG TVESPPPEAQEAPSLAPKVPPRRKKSAPAAFHLQVLQNNSQVLQGLTCSSSSPPSLKPDTHPLCLQVALG TSSARSPETHGPRVTEPEAASFHGNYPDPFWSLLHHPKLLNNTWLSKSSEPLDVGSRNPERTHTEPAQVN ASLAERGLPPDHGGKDLSHWVTASNKDKRTTLGV SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058749
ORF Size	4302 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058749, AAH58749
RefSeq Size	4841 bp
RefSeq ORF	4304 bp
Locus ID	20975
Gene Summary	Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis (By similarity).[UniProtKB/Swiss-Prot Function]

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