Ncoa2 (BC053387) Mouse Tagged ORF Clone

CAT#: MG211963

  • TrueORF®

Ncoa2 (tGFP-tagged) - Mouse nuclear receptor coactivator 2 (cDNA clone MGC:60676 IMAGE:30054358)



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Mouse Protein / Lysate Request

CNY 15,300.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 9530095N19, GRIP-1, TIF2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211963 representing BC053387
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGGGATGGGAGAAAACACCTCTGACCCGTCCAGGGCAGAGACCAGAAAACGCAAGGAATGTCCCG
ACCAGCTCGGACCCAGCCCCAAAAGGAGCACTGAGAAACGGAACCGCGAGCAGGAGAATAAGTACATAGA
GGAGCTGGCCGAGCTGATCTTCGCAAACTTTAATGATATTGACAACTTCAACTTCAAACCTGACAAATGT
GCCATCCTAAAAGAAACTGTGAAGCAGATCCGCCAGATCAAAGAGCAAGAGAAAGCAGCAGCTGCCAACA
TAGATGAAGTGCAGAAGTCAGATGTGTCGTCCACGGGGCAGGGTGTCATCGACAAGGATGCACTGGGGCC
CATGATGCTTGAGGCCCTCGATGGGTTCTTCTTCGTTGTGAACCTGGAAGGCAGTGTGGTGTTCGTGTCA
GAGAATGTGACACAGTATCTACGGTATAACCAAGAAGAGCTGATGAACAAGAGTGTCTACAGCATCCTGC
ATGTCGGGGACCACACTGAATTTGTCAAGAACCTGCTGCCAAAGTCCATGGTGAATGGAGGATCCTGGTC
TGGAGAACCTCCCAGGCGGAGCAGCCATACCTTCAACTGTCGCATGCTGGTGAAGCCTTTGCCAGATTCA
GAAGAGGAAGGCCATGATAGCCAGGAAGCCCATCAGAAATACGAGGCGATGCAGTGCTTCGCTGTGTCTC
AGCCCAAGTCCATCAAAGAGGAAGGCGAAGATTTGCAGTCCTGCTTGATTTGTGTGGCACGAAGAGTCCC
CATGAAGGAAAGACCAACTCTTCCCTCATCAGAAAGCTTTACCACCCGCCAGGACCTCCAAGGCAAGATC
ACTTCACTGGACACTAGCACCATGAGAGCCGCCATGAAGCCGGGCTGGGAAGATCTGGTAAGAAGATGCA
TTCAGAAGTTCCACACACAGCATGAAGGGGAGTCTCTATCATATGCCAAGAGGCATCACCATGAAGTTCT
GAGACAAGGGTTGGCGTTCAGTCAGATCTATCGTTTTTCTTTGTCTGATGGCACTCTCGTTGCTGCACAA
ACCAAGAGCAAACTCATCCGTTCTCAGACTACTAATGAGCCTCAGCTTGTAATATCTTTACACATGCTTC
ACAGAGAGCAGAATGTATGTGTAATGAATCCGGATCTGACTGGACAAGCGATGGGGAAGCCATTGAATCC
AATTAGCTCTAGCAGCCCTGCCCACCAGGCCCTGTGCAGTGGGAACCCAGGTCAGGACATGACCCTCGGT
AGCAATATAAATTTTCCCATGAATGGCCCAAAGGAACAAATGGGCATGCCTATGGGCAGGTTTGGTGGTT
CTGGGGGCATGAACCATGTGTCAGGCATGCAGGCAACCACTCCTCAGGGTAGTAACTATGCACTCAAAAT
GAACAGTCCCTCGCAAAGCAGCCCCGGCATGAACCCGGGGCAAGCCAGCTCCGTGCTCTCCCCAAGGCAG
CGCATGAGCCCCGGCGTGGCTGGCAGTCCTCGCATCCCACCCAGTCAGTTTTCCCCTGCAGGAAGCTTGC
ATTCCCCTGTGGGAGTTTGCAGCAACACAGGAAATAGCCATAGTTATACCAACAGTTCCCTCAATGCACT
GCAAGCCCTCAGCGAGGGCCATGGGGTCTCACTCGGGTCCTCGCTGGCTTCACCGGACCTAAAAATGGGC
AATTTGCAAAACTCCCCAGTTAATATGAATCCTCCCCCACTCAGCAAGATGGGAAGCTTGGACTCCAAAG
ACTGTTTTGGACTTTATGGGGAGCCCTCAGAAGGTACAACTGGACAAGCAGAGGCCAGCTGCCATCCTGA
AGAACAAAAGGGGCCCAATGATTCCAGCATGCCCCAGGCGGCCAGCGGGGACAGGGCTGAGGGACACAGC
CGGCTGCATGACAGCAAAGGGCAGACCAAACTCCTGCAGCTGCTGACCACCAAGTCCGACCAGATGGAGC
CTTCACCCTTGCCCAGCTCCTTGTCGGACACAAACAAGGACTCAACAGGGAGCTTGCCTGGGCCTGGGTC
CACGCATGGCACCTCGCTCAAGGAGAAGCATAAGATTTTGCACAGACTCTTACAGGACAGCAGTTCCCCT
GTGGACTTGGCCAAGCTGACAGCAGAAGCCACAGGCAAAGAGCTGAGCCAGGAGTCCAGCAGCACAGCTC
CTGGGTCGGAAGTGACTGTCAAACAGGAGCCAGCGAGCCCCAAGAAGAAAGAGAATGCACTACTGCGCTA
TTTGCTCGACAAAGATGATACTAAAGATATTGGTTTACCGGAAATAACCCCCAAACTCGAGCGACTGGAC
AGTAAGACAGATCCTGCCAGTAACACAAAGTTAATTGCTATGAAAACTGTGAAGGAGGAGGTGAGCTTTG
AGCCCAGTGACCAGCCTGGCAGCGAGCTGGACAACTTGGAAGAGATTTTGGATGATTTGCAGAACAGTCA
GTTACCACAGCTTTTCCCAGACACAAGGCCAGGAGCTCCTACTGGGTCAGTTGACAAGCAAGCCATCATC
AATGACCTCATGCAACTCACAGCTGACAGCAGTCCCGTCCCACCTGCCGGAGCCCAGAAGGCAGCACTGC
GCATGTCACAGAGCAGAATGATTGGCAGCAGCACTTCCCGGCCCAGCATGCCTTCTGGGGAATGGGCACC
ACAGAGTCCAGCTGTGAGAGTCACTTGTGCTGCTACCACTGGTGCCATGAACCGACCAGTCCAAGGAGGC
ATGATTCGGAACCCAACAGCCAGCATCCCCATGCGAGCCAACAGCCAGCCTGGCCAAAGACAGATGCTTC
AGTCTCAGGTCATGAACATAGGCCCTTCTGAGTTAGAGATGAACATGGGAGGACCTCAGTATAATCAACA
GCAGGCCCCTCCGAACCAAACTGCCCCGTGGCCTGAGAGCATCCTGCCTATAGACCAGGCATCGTTTGCC
AGCCAGAACAGGCAGCCCTTCGGCAGCTCCCCTGATGACCTGCTGTGTCCACATCCTGCAGCAGAGTCGC
CAAGCGATGAGGGCGCTCTTCTTGACCAGCTGTATCTGGCCTTGCGGAACTTCGATGGCCTTGAGGAGAT
TGATAGAGCTCTGGGGATACCAGAACTGGTCAGCCAGAGCCAAGCTGTGGATGCAGAGCAGTTCTCAAGT
CAGGAGTCCAGCATAATGCTGGAGCAGAAGCCCCCCGTTTTCCCACAGCAGTACGCATCTCAGGCACAAA
TGGCCCAGGGTGGCTATAATCCCATGCAAGATCCAAACTTTCACACCATGGGACAGCGGCCAAATTACAC
CACACTCCGTATGCAGCCACGGCCAGGCCTCAGGCCCACAGGCATTGTACAGAACCAGCCAAACCAACTG
AGACTTCAGCTTCAGCACCGCCTCCAAGCACAGCAGAATCGCCAGCCGCTTATGAATCAGATCAGCAGTG
TTTCCAATGTGAACCTGACTCTGAGGCCTGGAGTGCCCACTCAGGCTCCTATTAATGCACAGATGCTGGC
CCAGAGGCAGAGGGAAATCCTCAACCAACACCTTCGGCAGAGGCAGATGCAGCAGCAGGTGCAGCAGCGG
ACTCTGATGATGAGAGGACAGGGCTTGAATGTGACCCCAAGCATGGTGGCTCCCGCTGGCCTACCAGCAG
CCATGAGCAATCCCCGGATCCCCCAGGCCAATGCCCAGCAGTTCCCATTTCCTCCGAACTACGGAATAAG
TCAACAACCTGATCCTGGCTTTACTGGGGCTACGACTCCCCAGAGTCCTCTAATGTCTCCCCGGATGGCA
CATACTCAGAGTCCCATGATGCAGCAGTCTCAAGCCAACCCAGCCTACCAGCCCACCTCAGACATGAATG
GATGGGCACAGGGGAGCATGGGTGGAAACAGCATGTTCTCACAGCAGTCCCCACCACACTTTGGGCAACA
AGCAAACACCAGCATGTATAGTAACAACATGAACATCAGTGTGTCGATGGCAACCAACACGGGTGGCTTG
AGCAGCATGAACCAGATGACAGGCCAGATGAGCATGACCTCAGTGACCTCCGTGCCTACGTCAGGACTGC
CCTCCATGGGTCCCGAGCAGGTCAATGACCCTGCTCTGAGGGGAGGCAACCTTTTCCCAAACCAACTGCC
TGGAATGGACATGATCAAGCAGGAGGGAGATGCATCTCGGAAATACTGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211963 representing BC053387
Red=Cloning site Green=Tags(s)

MSGMGENTSDPSRAETRKRKECPDQLGPSPKRSTEKRNREQENKYIEELAELIFANFNDIDNFNFKPDKC
AILKETVKQIRQIKEQEKAAAANIDEVQKSDVSSTGQGVIDKDALGPMMLEALDGFFFVVNLEGSVVFVS
ENVTQYLRYNQEELMNKSVYSILHVGDHTEFVKNLLPKSMVNGGSWSGEPPRRSSHTFNCRMLVKPLPDS
EEEGHDSQEAHQKYEAMQCFAVSQPKSIKEEGEDLQSCLICVARRVPMKERPTLPSSESFTTRQDLQGKI
TSLDTSTMRAAMKPGWEDLVRRCIQKFHTQHEGESLSYAKRHHHEVLRQGLAFSQIYRFSLSDGTLVAAQ
TKSKLIRSQTTNEPQLVISLHMLHREQNVCVMNPDLTGQAMGKPLNPISSSSPAHQALCSGNPGQDMTLG
SNINFPMNGPKEQMGMPMGRFGGSGGMNHVSGMQATTPQGSNYALKMNSPSQSSPGMNPGQASSVLSPRQ
RMSPGVAGSPRIPPSQFSPAGSLHSPVGVCSNTGNSHSYTNSSLNALQALSEGHGVSLGSSLASPDLKMG
NLQNSPVNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAEASCHPEEQKGPNDSSMPQAASGDRAEGHS
RLHDSKGQTKLLQLLTTKSDQMEPSPLPSSLSDTNKDSTGSLPGPGSTHGTSLKEKHKILHRLLQDSSSP
VDLAKLTAEATGKELSQESSSTAPGSEVTVKQEPASPKKKENALLRYLLDKDDTKDIGLPEITPKLERLD
SKTDPASNTKLIAMKTVKEEVSFEPSDQPGSELDNLEEILDDLQNSQLPQLFPDTRPGAPTGSVDKQAII
NDLMQLTADSSPVPPAGAQKAALRMSQSRMIGSSTSRPSMPSGEWAPQSPAVRVTCAATTGAMNRPVQGG
MIRNPTASIPMRANSQPGQRQMLQSQVMNIGPSELEMNMGGPQYNQQQAPPNQTAPWPESILPIDQASFA
SQNRQPFGSSPDDLLCPHPAAESPSDEGALLDQLYLALRNFDGLEEIDRALGIPELVSQSQAVDAEQFSS
QESSIMLEQKPPVFPQQYASQAQMAQGGYNPMQDPNFHTMGQRPNYTTLRMQPRPGLRPTGIVQNQPNQL
RLQLQHRLQAQQNRQPLMNQISSVSNVNLTLRPGVPTQAPINAQMLAQRQREILNQHLRQRQMQQQVQQR
TLMMRGQGLNVTPSMVAPAGLPAAMSNPRIPQANAQQFPFPPNYGISQQPDPGFTGATTPQSPLMSPRMA
HTQSPMMQQSQANPAYQPTSDMNGWAQGSMGGNSMFSQQSPPHFGQQANTSMYSNNMNISVSMATNTGGL
SSMNQMTGQMSMTSVTSVPTSGLPSMGPEQVNDPALRGGNLFPNQLPGMDMIKQEGDASRKYC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC053387
ORF Size 4181 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC053387, AAH53387
RefSeq Size 6085 bp
RefSeq ORF 4181 bp
Locus ID 17978
Gene Summary Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (PubMed:24529706).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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