Ncoa2 (BC053387) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	9530095N19, GRIP-1, TIF2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211963 representing BC053387 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGGATGGGAGAAAACACCTCTGACCCGTCCAGGGCAGAGACCAGAAAACGCAAGGAATGTCCCG ACCAGCTCGGACCCAGCCCCAAAAGGAGCACTGAGAAACGGAACCGCGAGCAGGAGAATAAGTACATAGA GGAGCTGGCCGAGCTGATCTTCGCAAACTTTAATGATATTGACAACTTCAACTTCAAACCTGACAAATGT GCCATCCTAAAAGAAACTGTGAAGCAGATCCGCCAGATCAAAGAGCAAGAGAAAGCAGCAGCTGCCAACA TAGATGAAGTGCAGAAGTCAGATGTGTCGTCCACGGGGCAGGGTGTCATCGACAAGGATGCACTGGGGCC CATGATGCTTGAGGCCCTCGATGGGTTCTTCTTCGTTGTGAACCTGGAAGGCAGTGTGGTGTTCGTGTCA GAGAATGTGACACAGTATCTACGGTATAACCAAGAAGAGCTGATGAACAAGAGTGTCTACAGCATCCTGC ATGTCGGGGACCACACTGAATTTGTCAAGAACCTGCTGCCAAAGTCCATGGTGAATGGAGGATCCTGGTC TGGAGAACCTCCCAGGCGGAGCAGCCATACCTTCAACTGTCGCATGCTGGTGAAGCCTTTGCCAGATTCA GAAGAGGAAGGCCATGATAGCCAGGAAGCCCATCAGAAATACGAGGCGATGCAGTGCTTCGCTGTGTCTC AGCCCAAGTCCATCAAAGAGGAAGGCGAAGATTTGCAGTCCTGCTTGATTTGTGTGGCACGAAGAGTCCC CATGAAGGAAAGACCAACTCTTCCCTCATCAGAAAGCTTTACCACCCGCCAGGACCTCCAAGGCAAGATC ACTTCACTGGACACTAGCACCATGAGAGCCGCCATGAAGCCGGGCTGGGAAGATCTGGTAAGAAGATGCA TTCAGAAGTTCCACACACAGCATGAAGGGGAGTCTCTATCATATGCCAAGAGGCATCACCATGAAGTTCT GAGACAAGGGTTGGCGTTCAGTCAGATCTATCGTTTTTCTTTGTCTGATGGCACTCTCGTTGCTGCACAA ACCAAGAGCAAACTCATCCGTTCTCAGACTACTAATGAGCCTCAGCTTGTAATATCTTTACACATGCTTC ACAGAGAGCAGAATGTATGTGTAATGAATCCGGATCTGACTGGACAAGCGATGGGGAAGCCATTGAATCC AATTAGCTCTAGCAGCCCTGCCCACCAGGCCCTGTGCAGTGGGAACCCAGGTCAGGACATGACCCTCGGT AGCAATATAAATTTTCCCATGAATGGCCCAAAGGAACAAATGGGCATGCCTATGGGCAGGTTTGGTGGTT CTGGGGGCATGAACCATGTGTCAGGCATGCAGGCAACCACTCCTCAGGGTAGTAACTATGCACTCAAAAT GAACAGTCCCTCGCAAAGCAGCCCCGGCATGAACCCGGGGCAAGCCAGCTCCGTGCTCTCCCCAAGGCAG CGCATGAGCCCCGGCGTGGCTGGCAGTCCTCGCATCCCACCCAGTCAGTTTTCCCCTGCAGGAAGCTTGC ATTCCCCTGTGGGAGTTTGCAGCAACACAGGAAATAGCCATAGTTATACCAACAGTTCCCTCAATGCACT GCAAGCCCTCAGCGAGGGCCATGGGGTCTCACTCGGGTCCTCGCTGGCTTCACCGGACCTAAAAATGGGC AATTTGCAAAACTCCCCAGTTAATATGAATCCTCCCCCACTCAGCAAGATGGGAAGCTTGGACTCCAAAG ACTGTTTTGGACTTTATGGGGAGCCCTCAGAAGGTACAACTGGACAAGCAGAGGCCAGCTGCCATCCTGA AGAACAAAAGGGGCCCAATGATTCCAGCATGCCCCAGGCGGCCAGCGGGGACAGGGCTGAGGGACACAGC CGGCTGCATGACAGCAAAGGGCAGACCAAACTCCTGCAGCTGCTGACCACCAAGTCCGACCAGATGGAGC CTTCACCCTTGCCCAGCTCCTTGTCGGACACAAACAAGGACTCAACAGGGAGCTTGCCTGGGCCTGGGTC CACGCATGGCACCTCGCTCAAGGAGAAGCATAAGATTTTGCACAGACTCTTACAGGACAGCAGTTCCCCT GTGGACTTGGCCAAGCTGACAGCAGAAGCCACAGGCAAAGAGCTGAGCCAGGAGTCCAGCAGCACAGCTC CTGGGTCGGAAGTGACTGTCAAACAGGAGCCAGCGAGCCCCAAGAAGAAAGAGAATGCACTACTGCGCTA TTTGCTCGACAAAGATGATACTAAAGATATTGGTTTACCGGAAATAACCCCCAAACTCGAGCGACTGGAC AGTAAGACAGATCCTGCCAGTAACACAAAGTTAATTGCTATGAAAACTGTGAAGGAGGAGGTGAGCTTTG AGCCCAGTGACCAGCCTGGCAGCGAGCTGGACAACTTGGAAGAGATTTTGGATGATTTGCAGAACAGTCA GTTACCACAGCTTTTCCCAGACACAAGGCCAGGAGCTCCTACTGGGTCAGTTGACAAGCAAGCCATCATC AATGACCTCATGCAACTCACAGCTGACAGCAGTCCCGTCCCACCTGCCGGAGCCCAGAAGGCAGCACTGC GCATGTCACAGAGCAGAATGATTGGCAGCAGCACTTCCCGGCCCAGCATGCCTTCTGGGGAATGGGCACC ACAGAGTCCAGCTGTGAGAGTCACTTGTGCTGCTACCACTGGTGCCATGAACCGACCAGTCCAAGGAGGC ATGATTCGGAACCCAACAGCCAGCATCCCCATGCGAGCCAACAGCCAGCCTGGCCAAAGACAGATGCTTC AGTCTCAGGTCATGAACATAGGCCCTTCTGAGTTAGAGATGAACATGGGAGGACCTCAGTATAATCAACA GCAGGCCCCTCCGAACCAAACTGCCCCGTGGCCTGAGAGCATCCTGCCTATAGACCAGGCATCGTTTGCC AGCCAGAACAGGCAGCCCTTCGGCAGCTCCCCTGATGACCTGCTGTGTCCACATCCTGCAGCAGAGTCGC CAAGCGATGAGGGCGCTCTTCTTGACCAGCTGTATCTGGCCTTGCGGAACTTCGATGGCCTTGAGGAGAT TGATAGAGCTCTGGGGATACCAGAACTGGTCAGCCAGAGCCAAGCTGTGGATGCAGAGCAGTTCTCAAGT CAGGAGTCCAGCATAATGCTGGAGCAGAAGCCCCCCGTTTTCCCACAGCAGTACGCATCTCAGGCACAAA TGGCCCAGGGTGGCTATAATCCCATGCAAGATCCAAACTTTCACACCATGGGACAGCGGCCAAATTACAC CACACTCCGTATGCAGCCACGGCCAGGCCTCAGGCCCACAGGCATTGTACAGAACCAGCCAAACCAACTG AGACTTCAGCTTCAGCACCGCCTCCAAGCACAGCAGAATCGCCAGCCGCTTATGAATCAGATCAGCAGTG TTTCCAATGTGAACCTGACTCTGAGGCCTGGAGTGCCCACTCAGGCTCCTATTAATGCACAGATGCTGGC CCAGAGGCAGAGGGAAATCCTCAACCAACACCTTCGGCAGAGGCAGATGCAGCAGCAGGTGCAGCAGCGG ACTCTGATGATGAGAGGACAGGGCTTGAATGTGACCCCAAGCATGGTGGCTCCCGCTGGCCTACCAGCAG CCATGAGCAATCCCCGGATCCCCCAGGCCAATGCCCAGCAGTTCCCATTTCCTCCGAACTACGGAATAAG TCAACAACCTGATCCTGGCTTTACTGGGGCTACGACTCCCCAGAGTCCTCTAATGTCTCCCCGGATGGCA CATACTCAGAGTCCCATGATGCAGCAGTCTCAAGCCAACCCAGCCTACCAGCCCACCTCAGACATGAATG GATGGGCACAGGGGAGCATGGGTGGAAACAGCATGTTCTCACAGCAGTCCCCACCACACTTTGGGCAACA AGCAAACACCAGCATGTATAGTAACAACATGAACATCAGTGTGTCGATGGCAACCAACACGGGTGGCTTG AGCAGCATGAACCAGATGACAGGCCAGATGAGCATGACCTCAGTGACCTCCGTGCCTACGTCAGGACTGC CCTCCATGGGTCCCGAGCAGGTCAATGACCCTGCTCTGAGGGGAGGCAACCTTTTCCCAAACCAACTGCC TGGAATGGACATGATCAAGCAGGAGGGAGATGCATCTCGGAAATACTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211963 representing BC053387 Red=Cloning site Green=Tags(s) MSGMGENTSDPSRAETRKRKECPDQLGPSPKRSTEKRNREQENKYIEELAELIFANFNDIDNFNFKPDKC AILKETVKQIRQIKEQEKAAAANIDEVQKSDVSSTGQGVIDKDALGPMMLEALDGFFFVVNLEGSVVFVS ENVTQYLRYNQEELMNKSVYSILHVGDHTEFVKNLLPKSMVNGGSWSGEPPRRSSHTFNCRMLVKPLPDS EEEGHDSQEAHQKYEAMQCFAVSQPKSIKEEGEDLQSCLICVARRVPMKERPTLPSSESFTTRQDLQGKI TSLDTSTMRAAMKPGWEDLVRRCIQKFHTQHEGESLSYAKRHHHEVLRQGLAFSQIYRFSLSDGTLVAAQ TKSKLIRSQTTNEPQLVISLHMLHREQNVCVMNPDLTGQAMGKPLNPISSSSPAHQALCSGNPGQDMTLG SNINFPMNGPKEQMGMPMGRFGGSGGMNHVSGMQATTPQGSNYALKMNSPSQSSPGMNPGQASSVLSPRQ RMSPGVAGSPRIPPSQFSPAGSLHSPVGVCSNTGNSHSYTNSSLNALQALSEGHGVSLGSSLASPDLKMG NLQNSPVNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAEASCHPEEQKGPNDSSMPQAASGDRAEGHS RLHDSKGQTKLLQLLTTKSDQMEPSPLPSSLSDTNKDSTGSLPGPGSTHGTSLKEKHKILHRLLQDSSSP VDLAKLTAEATGKELSQESSSTAPGSEVTVKQEPASPKKKENALLRYLLDKDDTKDIGLPEITPKLERLD SKTDPASNTKLIAMKTVKEEVSFEPSDQPGSELDNLEEILDDLQNSQLPQLFPDTRPGAPTGSVDKQAII NDLMQLTADSSPVPPAGAQKAALRMSQSRMIGSSTSRPSMPSGEWAPQSPAVRVTCAATTGAMNRPVQGG MIRNPTASIPMRANSQPGQRQMLQSQVMNIGPSELEMNMGGPQYNQQQAPPNQTAPWPESILPIDQASFA SQNRQPFGSSPDDLLCPHPAAESPSDEGALLDQLYLALRNFDGLEEIDRALGIPELVSQSQAVDAEQFSS QESSIMLEQKPPVFPQQYASQAQMAQGGYNPMQDPNFHTMGQRPNYTTLRMQPRPGLRPTGIVQNQPNQL RLQLQHRLQAQQNRQPLMNQISSVSNVNLTLRPGVPTQAPINAQMLAQRQREILNQHLRQRQMQQQVQQR TLMMRGQGLNVTPSMVAPAGLPAAMSNPRIPQANAQQFPFPPNYGISQQPDPGFTGATTPQSPLMSPRMA HTQSPMMQQSQANPAYQPTSDMNGWAQGSMGGNSMFSQQSPPHFGQQANTSMYSNNMNISVSMATNTGGL SSMNQMTGQMSMTSVTSVPTSGLPSMGPEQVNDPALRGGNLFPNQLPGMDMIKQEGDASRKYC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC053387
ORF Size	4181 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC053387, AAH53387
RefSeq Size	6085 bp
RefSeq ORF	4181 bp
Locus ID	17978
Gene Summary	Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (PubMed:24529706).[UniProtKB/Swiss-Prot Function]

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