Dhx57 (BC062952) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC77974
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211933 representing BC062952 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCTTCAGTAAGAAGAAAAGGCAAGCCAGGCAAAGGGGATGGAAAGGGATCGTCTAGAGGCGGAA GAGGAGGCAAAGGTCACATGAATAAATCTCATGGGGGTGGAGGTGGCGGCGGCGGTAGCTGCGGCGGCGG AGGCGGCGGCAGTAGAAAGGCCTCAAATAGAATTTGGGATGATGGCGATGACTTTTGTGTCTTCACTGAG CCCAAGCGCCCGTCCAGAAGAGGCACCTCTGGGGAGGAGGAAGACAGCGAGCCCCAGTGTGGCGAGGAGC AGGGCTGGCCAGCTGGACAAGAGCCTATCTTTCTTCCTGACTGCAGTCCTTGGGAATATATAGGCCCAGA AGAAGTGGAGCCTCCTGTGCCAGAATGTGCAGTCTCACCCTTGGCAGTGCAGAAACTTTCCAGGTACGGC TTCCACACTGAGCACTGTCAGCTGGCCCTGAGGATCTGTGATGGAGACTTAGGGGCGGCACTAGAGCATC TTCTCCGCCAGTGTTTCTCAGAGACGTTTGGAGAGAGGATGGCGCTCTCTGAGGCAGCTGTTTATGTGAG CTTGAATGAGTGTGTGGAGCAGAGACAGGAAGAGACGCTTGCTCTCAAGTCCATCTGCGGAGAAAAATTC ATAGAAAGAATTCAGAACAGAGTCTGGACCATTGGATTGGAACTGGATTATTTGACAAACAAATTCTGCA AGTCTAAGCAAAAGGAAAGTAGCAAAAATGTACGGGACACTTCGCCTGAAACCTGTAAATTTTACCTCAA AGGAAATTGTAAATTTGGATCAAAATGCAAATTCAAACATGAAGTGCCCCCACATCAAATGATTGGGAGA GCAGAAAGAAACGTAAATGACCCTCACCTTGACGCTGATGACGACACAACCTTTATGTATGAGCTTCAGA TTCGCTTTTCTAAAGACCACAAATACCCCTACCAAGCTCCCCTGGTGGCATTTTATTCCACCAATGAGAA CCTACCTCTGGCTTGTCGTTTACATATTTCTGAGTTCCTTTATGGCAAGGCCTTGGAATTTGCAAAAACT TCAGAACCTGTTGTCTATTCTCTGATCACCCTTTTAGAGGAAGAGTCAGAGATTGTCAAGTTACTAACAC ACACTCAACATAAGTATAGCGTTCCTCCTGTGAACGTCCCTCCAGTACCTTCCGAGACCAGAATAAGTAA ACCTGCCTACCGCAAACCAGTGGTCCCAAGTAACACTTTCCTTTCTAATCAAATGCTAGAAGGGGAAAGA TTGTCAGAACTCGAGGAGGATGCCGATGAGGATGAAGGACCTGCATCAATTATCGTGGAGAATGAAAGCT ATGTGAACCTGAAGAAGAGGAGTTACAAAAGATACGACAGGCCGGCAAAGCCCTTATTTGCTGAGAACAG TAAGATCTGCAGGCAGTTTCAGATGAAACAGGCTTCCAGACAGTTCCACGCAATTCTGCAAGAAAGACAG TTACTGCCTGCTTGGGAAGAGAGAGAGACCATTCTGAAACTGCTGAGCAAGCACCAAGTGGTTGTCATAA GTGGTATGACTGGATGTGGGAAAACCACACAGATTCCACAGTTTATCCTGGATAATTCTCTGAATGGGCC ACCCGAGAGGGTGGCCAACATCATCTGTACCCAACCCCGACGGATCTCTGCAATCTCTGTTGCTGAACGG GTTGCTAAAGAAAGAGCAGAACGGGTGGGTCTGACTGTGGGATACCAGATTCGGCTGGAAAGTGTCAAGT CCTCAGCCACCAGACTGTTATACTGCACCACAGGAGTGCTGCTGAGAAGGCTAGAGGGAGATGCGACTTT GCAGGGAGTCACCCACATCATTGTTGATGAAGTTCATGAGAGGACAGAAGAAAGTGACTTCTTACTGCTA GTTTTGAAGGACATTGTGATGCAGAGAGCGACCCTCCAGGTCATTCTCATGAGTGCAACTCTGGACGCTG GGCTGTTCTCCAAGTATTTCAGCTACTGCCCAGTCATCACCATCCCAGGCCGTGCATTTCCTGTTGATCA GTTTTTCCTGGAGGATGCGCTGGCTGTGACGAGGTATGTGTTACAGGACGGGAGCCCATATATGCGTTCC ATGAAACAGATTGCGAAGGAAAAGCTTAAAGCCAGGCACAACAGAACTGCACAGGAGGAGGTAGAGGAAG ACCTGAGGCTGTCCCTCCATCTCCAGGACGAGGAGGAGTCTGTCAAAGATACAATACCTGATCAACAGTT GGATTTCAAGCAGCTCTTGATCCGTTATAAAGGGGTGAGCAAGTCAGTCATCAAGACAATGTCTGTGATG GATTTTGAAAAGGTCAACCTTGAACTAATAGAGGCCTTGTTAGAGTGGATTGTAGACGGCAAGCACGCCT ACCCTCCAGGCGCTGTCCTTGTGTTCTTACCAGGGCTGGCAGAAATCAAGATGCTTTATGAGCAGCTACA GTCTAACTCTCTTTTCAACAACAGACGCAGTCACCGATGTGTAATTCACCCGCTGCACTCATCATTATCC AGCGAAGAGCAGCAGGCGGTGTTTGTGAAACCTCCAATGGGTGTAACTAAGATTATCATTTCCACCAACA TTGCTGAGACGTCTATAACCATTGATGATGTTGTGTATGTCATCGATTCTGGGAAGATGAAAGAAAAGAG ATACGACGCAGGCAAAGGAATGGAAAGTCTAGAGGACACGTTCGTGTCTCAGGCCAACGCTCTGCAGAGG AAAGGCCGAGCTGGCCGAGTCGCATCAGGGGTCTGCTTCCACTTGTTCACGAGCCACCACTACAACCACC AGCTTCTGAAGCAGCAGCTCCCAGAAATCCAGCGAGTGCCCCTGGAGCAGCTGTGCCTCAGAATTAAAAT TTTAGAAATGTTTAGTACTCATAATCTCCAGTCCGTGTTCTCTCGGCTCATTGAACCTCCACATATTGAT TCTCTTCGTGCCTCAAAAGTACGGTTGCGAGACTTGGGAGCCTTGACTCCAGATGAAAAACTGACCCCGC TGGGTTATCACTTGGCCTCTCTGCCCGTGGACGTGAGGATAGGCAAGCTGATGCTCTTAGGTTCTATCTT CCGCTGTTTGGACCCTGCTCTCACCATTGCTGCCAGCTTGGCTTTCAAGTCCCCTTTTGTGTCTCCCTGG GATAAGAAAGAAGAAGCTAATCAGAAAAAGCTGGAGTTTGCATTTGCAAACAGCGATTACCTGGCCCTTC TATGTGCATATAAGGGTTGGCAGCTGAGTACAAAAGAAAGCGCACGAGCAAGCTATAATTACTGCAGACA GAATTTCCTGTCTGGGAGAACCCTGCAGGAAATGGCCAGCCTCAAACGACAATTCACTGAACTGCTGTCC GACATAGGGTTTGTGAAAGAAGGACTCCGAGCAAAAGAAATTGAAAAACGAGCCCAGGGAGGAGATGGTG TCCTGGATGCCACAGGAGAGGAGGCTAACACAAATGCTGAGAACCCCAAGCTGATATCAGCAGTGCTGTG TGCCGCTCTGTATCCAAACGTAGTACAGGTCAAGACCCCAGAGGGAAAGTTCCAGAAGACCAGTTCTGGA GTGGTCAGACTGCAGCCAAAGTCAGCTGAGCTGAAGTTCGTTACCAAGAATGACGGATATGTCCACATTC ACCCTTCCTCAGTGAACTATCAGGTCAGACACTTCGACAGCCCCTACCTGTTGTACCATGAGAAAATCAA AACTAGCCGGGTGTTCATCCGAGACTGCAGCATGGTGTCTGTGTATCCCCTGGTCTTGTTTGGAGGGGGC CAAGTGAACGTGCAGCTTCAAAGAGGGGCGTTCGTCGTCTCCTTGGATGACGGCTGGATCCGGTTTGTGG CCGCCTCCCACCAGGTGGCCGAGCTGGTGAAGGAACTCCGCTGCGAACTTGACCAGCTCCTCCAGGATAA GATTAAAAACCCCAGCATGGACCTGTGCAGCTGTCCGAGGGGCTCTCGGATTATCAGCATGATCGTGAAG CTCATCACCACACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211933 representing BC062952 Red=Cloning site Green=Tags(s) MSSSVRRKGKPGKGDGKGSSRGGRGGKGHMNKSHGGGGGGGGSCGGGGGGSRKASNRIWDDGDDFCVFTE PKRPSRRGTSGEEEDSEPQCGEEQGWPAGQEPIFLPDCSPWEYIGPEEVEPPVPECAVSPLAVQKLSRYG FHTEHCQLALRICDGDLGAALEHLLRQCFSETFGERMALSEAAVYVSLNECVEQRQEETLALKSICGEKF IERIQNRVWTIGLELDYLTNKFCKSKQKESSKNVRDTSPETCKFYLKGNCKFGSKCKFKHEVPPHQMIGR AERNVNDPHLDADDDTTFMYELQIRFSKDHKYPYQAPLVAFYSTNENLPLACRLHISEFLYGKALEFAKT SEPVVYSLITLLEEESEIVKLLTHTQHKYSVPPVNVPPVPSETRISKPAYRKPVVPSNTFLSNQMLEGER LSELEEDADEDEGPASIIVENESYVNLKKRSYKRYDRPAKPLFAENSKICRQFQMKQASRQFHAILQERQ LLPAWEERETILKLLSKHQVVVISGMTGCGKTTQIPQFILDNSLNGPPERVANIICTQPRRISAISVAER VAKERAERVGLTVGYQIRLESVKSSATRLLYCTTGVLLRRLEGDATLQGVTHIIVDEVHERTEESDFLLL VLKDIVMQRATLQVILMSATLDAGLFSKYFSYCPVITIPGRAFPVDQFFLEDALAVTRYVLQDGSPYMRS MKQIAKEKLKARHNRTAQEEVEEDLRLSLHLQDEEESVKDTIPDQQLDFKQLLIRYKGVSKSVIKTMSVM DFEKVNLELIEALLEWIVDGKHAYPPGAVLVFLPGLAEIKMLYEQLQSNSLFNNRRSHRCVIHPLHSSLS SEEQQAVFVKPPMGVTKIIISTNIAETSITIDDVVYVIDSGKMKEKRYDAGKGMESLEDTFVSQANALQR KGRAGRVASGVCFHLFTSHHYNHQLLKQQLPEIQRVPLEQLCLRIKILEMFSTHNLQSVFSRLIEPPHID SLRASKVRLRDLGALTPDEKLTPLGYHLASLPVDVRIGKLMLLGSIFRCLDPALTIAASLAFKSPFVSPW DKKEEANQKKLEFAFANSDYLALLCAYKGWQLSTKESARASYNYCRQNFLSGRTLQEMASLKRQFTELLS DIGFVKEGLRAKEIEKRAQGGDGVLDATGEEANTNAENPKLISAVLCAALYPNVVQVKTPEGKFQKTSSG VVRLQPKSAELKFVTKNDGYVHIHPSSVNYQVRHFDSPYLLYHEKIKTSRVFIRDCSMVSVYPLVLFGGG QVNVQLQRGAFVVSLDDGWIRFVAASHQVAELVKELRCELDQLLQDKIKNPSMDLCSCPRGSRIISMIVK LITTQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC062952
ORF Size	4007 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC062952, AAH62952
RefSeq Size	5006 bp
RefSeq ORF	4007 bp
Locus ID	106794
Gene Summary	Probable ATP-binding RNA helicase.[UniProtKB/Swiss-Prot Function]

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