Pnpla6 (BC054789) Mouse Tagged ORF Clone

CAT#: MG211925

  • TrueORF®

Pnpla6 (tGFP-tagged) - Mouse patatin-like phospholipase domain containing 6 (cDNA clone MGC:65634 IMAGE:6831507)

ORF Plasmid: DDK tGFP



  "BC054789" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 14,540.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-Pnpla6 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI661849; MSws; Nte
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211925 representing BC054789
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCCCCACTGCAAACCGGAATGGTGCTGGGCGTGATGATCGGGGCCGGAGTGGCGGTGCTGGTCA
CCGCCGTGCTCATCCTCTTGGTGGTGCGAAGGCTGCGGGTGCAGAAAACTCCAGCCCCGGAGGGTCCCCG
GTATCGGTTCCGGAAGCGGGACAAAGTGCTTTTCTATGGCCGGAAGATTATGCGGAAGGTGTCACAGTCC
ACTTCTTCCCTGGTGGACACATCAGTCTCCACCACTTCCCGGCCCCGCATGAAGAAGAAACTGAAGATGC
TTAACATTGCCAAGAAGATCTTACGTATCCAAAAGGAGACTCCGACCCTACAACGGAAGGAGCCCCCACC
TTCAGTGCTGGAGGCTGACCTCACAGAGGGAGACCTGGCCAATTCCCACCTACCCTCTGAGGTACTCTAC
ATGCTTAAGAATGTTCGGGTGCTAGGTCACTTTGAGAAGCCGCTCTTCCTGGAGCTCTGCCGGCACATGG
TCTTCCAGCGTCTCGGGCAGGGGGACTATGTTTTTCGGCCCGGTCAACCAGATGCCAGCATCTATGTGGT
TCAAGATGGGCTGCTGGAGCTCTGCCTACCAGGGCCTGATGGGAAGGAGTGTGTGGTGAAGGAGGTGGTT
CCTGGAGACAGCGTCAACAGCCTTCTGAGCATCCTTGATGTCATCACGGGTCACCAGCATCCCCAGCGGA
CTGTGTCAGCCAGGGCTGCACGGGACTCCACAGTGCTGAGACTCCCAGTAGAAGCCTTCTCTGCTGTCTT
CACCAAGTACCCTGAGAGTTTGGTGCGAGTGGTACAGATCATCATGGTAAGGCTGCAGAGAGTCACCTTC
CTGGCGCTTCACAATTACCTGGGTCTGACCAATGAACTCTTCAGTCATGAGATCCAGCCCCTACGCCTTT
TCCCCAGCCCCGGCCTCCCGACCCGAACCAGCCCTGTCCGTGGCTCTAAACGGGTAGTCAGCACCTCAGG
TACTGAGGACACCTCAAAGGAGACCTCTGGCCGGCCCCTGGACTCCATTGGAGCTCCTCTGCCTGGACCT
GCAGGGGATCCCGTGAAGCCCACATCCTTAGAAGCACCCCCAGCCCCGTTACTGAGCCGCTGCATCTCCA
TGCCAGTAGACATCTCAGGCTTGCAAGGTGGCCCTCGTTCTGATTTTGACATGGCCTATGAACGTGGACG
GATATCTGTTTCTCTTCAAGAAGAGGCATCTGGGGGACCTCAGACAGCATCCCCTAGGGAGCTCCGGGAG
CAGCCAGCGGGTGCCTGTGAATATAGCTACTGTGAGGATGAGTCAGCCACAGGCGGATGTCCCTTTGGGC
CCTACCAGGGCCGCCAGACAAGCAGCATCTTTGAGGCTGCAAAGAGAGAGCTAGCCAAGCTGATGCGGAT
TGAGGACCCCTCTCTACTGAACAGCAGAGTCTTGCTACATCATGCCAAAGCCGGCACCATCATAGCCCGC
CAAGGGGACCAGGATGTGAGCCTGCACTTTGTGCTCTGGGGCTGCCTGCACGTGTACCAGCGCATGATTG
ACAAGGCTGAGGAAGTGTGCCTATTTGTGGCACAGCCAGGGGAGCTGGTGGGGCAACTGGCGGTGCTCAC
TGGGGAACCTCTCATCTTCACACTACGTGCCCAGAGAGACTGCACCTTCCTGCGGATCTCCAAATCCCAC
TTCTATGAGATCATGCGTGCACAACCCAGTGTGGTACTGAGTGCAGCTCACACGGTGGCTGCTAGAATGT
CCCCCTTCGTGCGCCAGATGGACTTTGCCATTGACTGGACAGCTGTGGAGGCTGGTCGTGCGCTCTACAG
GCAGGGAGACCGCTCCGACTGCACCTACATTGTACTCAATGGACGTCTCCGTAGTGTCATCCAACGAGGC
AGTGGCAAGAAGGAGCTAGTTGGGGAGTATGGTCGAGGGGATCTCATTGGCGTGGTGGAGGCACTGACCC
GGCAGCCACGAGCCACCACTGTACACGCAGTGCGAGACACTGAGCTGGCCAAGCTTCCCGAGGGCACCTT
AGGCCACATCAAACGTCGGTACCCACAGGTTGTGACCCGCCTTATTCATCTGCTAAGCCAGAAAATTCTA
GGCAATCTGCAGCAGTTGCAAGGACCCTTTCCAGGCTCCGGGCTCAGTGTTCCGCAGCACTCGGAACTGA
CCAACCCAGCCAGCAATCTGTCTACTGTAGCTATCCTCCCTGTGTGTGCTGAGGTGCCCATGATGGCCTT
TACTCTGGAACTGCAGCATGCTCTGCAAGCTATTGGTCCCACGCTCCTCCTTAACAGTGACGTCATCCGG
GCACTCCTGGGGGCTTCAGCACTGGATAGCATTCAAGAATTCCGGCTGTCAGGGTGGTTGGCCCAGCAGG
AAGATGCCCATCGCATTGTGCTCTACCAAACTGACACATCCCTGACGCCTTGGACCGTCCGTTGTCTGCG
CCAGGCCGACTGCATCCTCATTGTGGGCCTGGGGGACCAGGAACCCACTGTTGGCCAGCTGGAGCAAATG
TTAGAGAACACTGCTGTCCGTGCCTTGAAGCAACTGGTTTTGCTGCACCGGGAGGAAGGCCCTGGTCCCA
CGCGCACTGTAGAGTGGCTCAACATGCGCAGCTGGTGCTCAGGGCACCTGCACCTGCGCTGTCCTCGCCG
CCTCTTCTCACGGCGCAGCCCAGCGAAACTTCATGAGCTGTATGAGAAGGTTTTCTCCAGGCGTGCAGAC
CGTCACAGCGACTTCTCCCGCTTGGCACGAGTACTCACAGGAAACACTATTGCTCTGGTGCTGGGTGGGG
GCGGAGCCAGAGGCTGCTCGCATATTGGGGTGCTGAAGGCATTAGAGGAGGCAGGAGTCCCAGTCGACCT
TGTGGGAGGCACATCCATAGGTTCCTTCATTGGGGCCTTGTATGCCGAGGAACGCAGCGCCAGCCGAACT
AAACAACGAGCCCGGGAGTGGGCCAAGAGCATGACTTCTGTACTGGAGCCTGTATTGGACCTCACATATC
CTGTCACCTCCATGTTTACTGGCTCAGCCTTTAACAGAAGTATCCACCGTGTCTTCCAGGATAAGCAGAT
TGAGGACCTGTGGCTGCCTTACTTCAATGTGACCACAGACATCACTGCCTCTGCCATGCGCGTCCACAAA
GATGGCTCCCTGTGGCGGTATGTACGTGCCAGCATGACGCTCTCGGGCTACCTACCCCCGCTGTGCGACC
CAAAGGATGGGCACCTGCTCATGGATGGTGGCTACATCAACAACCTGCCAGCGGATATTGCCCGAAGCAT
GGGAGCCAAAACGGTCATTGCCATCGACGTTGGAAGCCAGGATGAGACAGATCTCAGCACCTATGGGGAC
AGCCTGTCTGGCTGGTGGTTGCTGTGGAAAAGGCTAAACCCCTGGGCAGACAAGGTAAAGGTTCCAGACA
TGGCTGAGATCCAGTCCCGCTTGGCGTACGTGTCCTGTGTGCGGCAGCTAGAAGTCGTCAAGTCCAGCTC
CTATTGCGAGTACCTTCGTCCATCCATTGACTGCTTCAAGACCATGGACTTTGGAAAGTTTGACCAGATC
TATGATGTGGGTTACCAGTATGGGAAGGCTGTCTTTGGAGGCTGGACCCGTGGTGAAGTCATTGAGAAAA
TGCTCACAGACCGGAGATCTACAGACCTTAATGAGAGTCGCCGTGCAGATATACTTGCCTTCCCAAGTTC
TGGGTTCACTGACTTGGCTGAGATTGTGTCCCGGATCGAGCCACCAACAAGCTACGTCTCTGATGGCTGT
GCTGATGGGGAGGAGTCGGATTGCTTGACAGAGTATGAGGAAGATGCAGGACCAGACTGCTCAAGAGATG
AAGGGGGCTCCCCTGAGGGTGCCAGCCCTAGCACTGCCTCAGAGGTGGAAGAAGAGAAGTCCACACTCCG
GCAACGACGCTTTCTGCCTCAGGAGACTCCCAGCTCAGTCGCAGATGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211925 representing BC054789
Red=Cloning site Green=Tags(s)

MEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQS
TSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLY
MLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVV
PGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTF
LALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGP
AGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELRE
QPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIAR
QGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSH
FYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRG
SGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKIL
GNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIR
ALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQM
LENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRAD
RHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRT
KQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHK
DGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGD
SLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQI
YDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGC
ADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC054789
ORF Size 3969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC054789.1
RefSeq Size 4461 bp
RefSeq ORF 3971 bp
Locus ID 50767
Gene Summary Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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