Zfp521 (BC021376) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	B930086A16Rik; Evi3; Znf521
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211913 representing BC021376 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGCCGCAAGCAAGCGAAACCGAGATCCCTCAAAGACCCCAACTGTAAACTTGAAGACAAGATTG AAGATGGGGAGGCAGTAGACTGCAAGAAGAGGCCAGAAGACGGGGAGGAGTTGGAGGAGGACGCTGTGCA CAGCTGTGACAGCTGCCTCCAGGTGTTTGAGTCACTGAGCGACATCACAGAGCACAAGATCCATCAGTGC CAGCTGACAGATGGCGTTGACGTTGAGGACGACCCCAGCTGCTCTTGGCCAGCCTCCTCGCCTTCCAGTA AGGACCAGACTTCCCCCAGCCATGGAGAAGGGTGTGATTTCGGAGAGGAAGAAGGTGGCCCTGGACTGCC TTACCCATGCCAGTTCTGTGACAAGTCCTTTAGCCGCCTCAGCTACCTAAAGCACCATGAACAGAGCCAT AGTGACAAGCTGCCCTTCAAGTGCACCTACTGCAGTCGGCTGTTCAAACACAAGCGGAGCCGTGACCGCC ACATAAAGCTCCACACTGGGGATAAGAAGTATCACTGTAGCGAGTGTGACGCAGCCTTCTCCCGGAGCGA TCATCTCAAGATCCACTTAAAGACTCACACCTCCAACAAGCCATATAAATGTGCCGTCTGCCGCAGAGGG TTCCTCTCCTCTAGCTCCTTGCACGGACATATGCAGGTTCATGAGCGGAACAAGGACGGCTCCCAGTCTG GCTCCAGGATGGAGGACTGGAAGATGAAGGACACTCAGAAGTGCAGCCAGTGCGAGGAAGGCTTTGACTT CCCGGAAGACCTCCAGAAGCACATTGCAGAATGCCACCCCGAGTGCTCCCCTAATGAGGACCGAGCAGCC CTCCAGTGCATGTACTGCCACGAGCTGTTTGTGGAGGAGACGTCCCTCATGAACCACATCGAGCAGGTGC ACGGGGGTGAGAAGAAGAACTCTTGCAGCATTTGCTCAGAGAGCTTCCTCACGGTCGAGGAGCTGTATAG CCACATGGACAGTCACCAGCAGCCAGAGTCCTGCAATCACAGCAACAGCCCTTCCCTGGTCACTGTGGGT TACACCTCAGTGTCCAGCACGACTCCAGACTCGAACCTCTCAGTGGACAGCTCGACCATGGTAGAGGCTG CACCACCGATTCCAAAGAGCCGCGGGAGGAAGCGAGCTGCTCAGCAGACCTCCGATATGACTGGCCCCTC TAGTAAGCAGGCGAAAGTCACCTACAGCTGTATTTACTGCAACAAACAGTTATTTTCCAGTCTCGCGGTT CTGCAGATTCACTTGAAAACTATGCATTTAGATAAGCCTGAGCAGGCTCATATCTGTCAGTATTGCTTGG AGGTCTTACCCTCACTCTATAACCTAAATGAACATCTTAAACAAGTGCACGAAGCTCAGGACCCCGGCCT GATTGTTTCGGCCATGCCTGCCATTGTTTACCAGTGCAACTTCTGTTCCGAAGTTGTCAATGACCTCAAC ACCCTTCAGGAGCACATCCGATGTTCTCATGGGTTTGCCAATCCCGCGGCCAAGGACAGCAATGCGTTCT TTTGTCCCCATTGTTACATGGGGTTTCTCACTGACTCTTCACTTGAAGAGCATATAAGACAGGTCCATTG TGACCTCAGTGGCTCCCGGTTTGGGTCTCCTGTGCTTGGGACTCCAAAAGAACCGGTGGTTGAAGTCTAC TCCTGTTCCTATTGTACAAATTCGCCAATATTCAACAGTGTTCTTAAACTGAATAAGCATATTAAAGAGA ATCATAAAAACATTCCCTTGGCCCTGAATTATATTCACAATGGGAAGAAATCCCGGGCCTTGAGCCCTTT GTCCCCTGTGGCTATAGAACAAACAACTCTTAAGATGATGCAGACTGTGGGAGGCGGGCCTGCCCGTGCC TCTGGAGAGTATATCTGTAATCAGTGTGGTGCTAAGTACACGTCCCTAGACAGCTTTCAGACTCACCTCA AAACCCATTTGGACACCGTGCTGCCAAAACTGACCTGCCCTCAGTGTAACAAAGAATTCCCCAACCAAGA GTCCTTGCTAAAGCACGTGACCATCCACTTTATGATCACCTCAACCTACTACATCTGTGAGAGCTGTGAC AAGCAGTTCACCTCCGTGGATGACCTCCAGAAGCACCTGCTGGACATGCACACCTTCGTCTTCTTCCGCT GCACACTCTGCCAGGAAGTGTTCGACTCCAAGGTCTCTATCCAGCTGCACTTGGCCGTGAAGCACAGTAA CGAGAAGAAGGTGTACCGCTGCACGTCCTGCAACTGGGACTTCCGCAACGAGACCGACCTGCAGCTGCAC GTGAAGCACAACCACTTGGAGAACCAAGGCAAAGTGCACAAGTGCATCTTCTGCGGCGAGTCGTTTGGCA CCGAGGTGGAGCTGCAGTGCCACATCACCACCCACAGCAAGAAGTACAACTGCAGGTTCTGCAGCAAGGC CTTCCACGCCGTCATCCTGCTGGAGAAGCACCTGCGGGAGAAACACTGTGTGTTTGAAACCAAGACCCCC AACTGTGGCACCAACGGGGCCTCGGAGCAAGTGCAGAAGGAGGAGGCTGAGCTGCAGACCTTGCTCACCA ACAGCCAGGAGTCCCATAACAGCCATGATGGGAGTGAGGAGGACGTGGACAGCTCTGAACCCATGTATGG CTGCGACATCTGCGGGGCGGCCTACACCATGGAGACGTTGCTGCAGAACCACCAGCTCCGAGACCACAAC ATCAGGCCTGGAGAAAGCGCCATCGTGAAGAAGAAGGCGGAGCTCATTAAAGGGAATTACAAGTGTAACG TTTGCTCGAGAACCTTCTTCTCCGAAAACGGGCTCCGTGAACACATGCAGACCCACTTAGGCCCGGTCAA ACACTACATGTGCCCTATCTGTGGAGAGCGCTTCCCTTCCCTATTAACTCTCACAGAGCACAAAGTCACA CACAGCAAGAGCCTGGATACCGGAAACTGTCGTATTTGTAAGATGCCCCTGCAGAGCGAAGAGGAGTTTT TGGAGCACTGCCAGATGCACCCTGACCTGAGGAATTCCCTGACTGGGTTTCGTTGTGTGGTGTGTATGCA GACTGTGACCTCCACCTTGGAACTCAAAATCCACGGGACCTTCCACATGCAGAAGACGGGGAATGGGTCC TCCGTGCAGACCACCGGGCGTGGCCAGCACGTTCAGAAACTGTATAAGTGCGCATCTTGCCTCAAAGAGT TCCGTTCCAAGCAAGATCTGGTGAAACTCGACATCAACGGCCTGCCATATGGTCTGTGTGCCGGCTGTGT GAATCTCAGTAAGAGCAGTAGCCCGGGCCTCAGTCTCCCTCCTGGTGCCAGCAGGCCAGGCTTGGGCCAG AATGAGAGTCTGAGCGCCATGGAAGGAAAAGGCAAGGCGGGGGGACTGAAGACGCGCTGTTCAAGCTGCA ATGTCAAGTTTGAGTCTGAAAGCGAACTGCAGAACCACATCCAGACGGTGCACCGGGAGCTTGTGCCAGA CGCCAACAGCACACAGTTGAAAACGCCCCAAGTGTCACCCATGCCCAGAATCAGTCCCTCCCAGTCCGAT GAGAAGAAGACCTACCAGTGTATCAAGTGTCAGATGGTTTTCTACAACGAGTGGGACATCCAGGTTCATG TGGCAAATCACATGATTGATGAAGGGCTGAACCATGAGTGCAAACTCTGCAGCCAGACCTTTGACTCCCC TGCCAAACTTCAGTGTCACCTGATAGAGCACAGCTTCGAAGGGATGGGTGGTACCTTCAAGTGCCCCGTC TGTTTCACAGTGTTTGTTCAAGCTAACAAGTTGCAACAGCATATATTCTCTGCCCATGGACAAGAAGACA AGATCTATGACTGCACACAATGCCCACAGAAGTTTTTCTTCCAAACAGAGCTACAGAATCATACGATGAC TCAGCACAGCAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211913 representing BC021376 Red=Cloning site Green=Tags(s) MSRRKQAKPRSLKDPNCKLEDKIEDGEAVDCKKRPEDGEELEEDAVHSCDSCLQVFESLSDITEHKIHQC QLTDGVDVEDDPSCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSH SDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAVCRRG FLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAA LQCMYCHELFVEETSLMNHIEQVHGGEKKNSCSICSESFLTVEELYSHMDSHQQPESCNHSNSPSLVTVG YTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTSDMTGPSSKQAKVTYSCIYCNKQLFSSLAV LQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLN TLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVY SCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTTLKMMQTVGGGPARA SGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCD KQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLH VKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCRFCSKAFHAVILLEKHLREKHCVFETKTP NCGTNGASEQVQKEEAELQTLLTNSQESHNSHDGSEEDVDSSEPMYGCDICGAAYTMETLLQNHQLRDHN IRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVT HSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGS SVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSSSPGLSLPPGASRPGLGQ NESLSAMEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDANSTQLKTPQVSPMPRISPSQSD EKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPV CFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC021376
ORF Size	3935 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021376, AAH21376
RefSeq Size	4781 bp
RefSeq ORF	3935 bp
Locus ID	225207
Gene Summary	Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes.[UniProtKB/Swiss-Prot Function]

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