Ncan (BC065118) Mouse Tagged ORF Clone

CAT#: MG211874

  • TrueORF®

Ncan (tGFP-tagged) - Mouse neurocan (cDNA clone MGC:86112 IMAGE:6853253)



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Mouse Protein / Lysate Request

CNY 13,870.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms C230035B04; Cspg3; Cspg3-rs; Tgfbit
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211874 representing BC065118
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCCGGATCTGTGTGGGCCTCAGGCCTCCTGCTGCTGTGGCTGCTTCTCCTAGTGGCTGGGGATC
AGGACACACAGGACACCACCGCCACGGAAAAGGGGCTTCGCATGCTGAAGTCAGGGTCAGGACCCGTCCG
TGCTGCCCTGGCAGAGCTAGTGGCCCTGCCCTGCTTCTTTACCCTGCAACCACGGCTAAGCTCCCTGCGA
GACATTCCTCGGATCAAGTGGACTAAGGTTCAGACTGCATCAGGCCAGCGACAGGATTTGCCAATCTTGG
TGGCCAAAGACAACGTGGTGCGTGTGGCCAAGGGCTGGCAGGGACGGGTGTCATTGCCTGCTTATCCCCG
GCACAGAGCCAATGCTACCCTTCTGCTGGGGCCACTTCGAGCAAGCGACTCTGGGCTGTATCGCTGCCAA
GTGGTAAAGGGTATCGAAGATGAGCAGGACCTGGTAACCCTGGAAGTGACAGGCGTCGTGTTCCATTATC
GGGCGGCCCGGGACCGCTATGCGCTGACCTTCGCTGAGGCCCAGGAGGCTTGTCGCCTAAGCTCTGCTAC
CATCGCTGCCCCACGGCACCTGCAGGCTGCCTTTGAAGATGGCTTTGACAACTGCGACGCGGGCTGGCTC
TCAGACCGCACGGTTCGGTACCCGATCACTCAGTCGCGTCCTGGTTGCTATGGTGACCGCAGCAGTCTCC
CGGGTGTTCGGAGCTACGGGAGACGCGACCCGCAGGAACTCTACGATGTCTACTGCTTTGCCCGCGAGCT
AGGGGGTGAAGTCTTTTACGTGGGCCCGGCCCGCCGACTGACCCTGGCGGGCGCGCGGGCACAATGTCAG
CGGCAGGGTGCAGCGCTGGCCTCGGTGGGGCAGTTGCACCTGGCCTGGCACGAGGGCCTGGACCAGTGCG
ACCCGGGCTGGCTGGCAGACGGCAGCGTGCGCTACCCCATCCAGACTCCGCGCCGGCGTTGCGGGGGCCC
CGCCCCAGGTGTGCGCACTGTGTACCGCTTCGCCAACCGCACCGGCTTTCCTGCGCCAGGAGCACGCTTC
GACGCCTACTGCTTCCGAGCTCATCACCATACAGCACAACATGGAGATTCTGAGATCCCCTCATCTGGAG
ATGAGGGGGAGATTGTGTCAGCAGAGGGGCCTCCAGGCCGAGAACTAAAGCCCAGCTTGGGGGAACAGGA
GGTGATAGCACCTGACTTCCAGGAACCTCTCATGTCCAGTGGAGAAGGCGAACCCCCAGACTTGACTTGG
ACACAAGCACCTGAGGAGACCCTTGGTTCTACTCCAGGGGGTCCCACGCTGGCCTCATGGCCCTCTTCAG
AAAAGTGGCTCTTTACGGGTGCCCCAAGCTCCATGGGTGTCTCCAGTCCCAGCGACATGGGAGTAGATAT
GGAAGCGACAACACCCTTGGGCACACAGGTAGCACCCACCCCCACGATGAGGAGGGGCCGCTTTAAAGGG
TTGAATGGTCGACACTTCCAGCAGCAGGGCCCAGAAGACCAGCTGCCTGAGGTAGCAGAGCCCAGTGCCC
AGCCTCCCACCCTGGGAGCTACTGCCAACCACATGAGGCCTTCTGCAGCCACAGAGGCTTCAGAGAGTGA
CCAGAGCCACAGTCCTTGGGCCATTCTGACCAACGAAGTGGATGAGCCAGGAGCAGGCTCTCTTGGCAGC
AGGAGTCTCCCAGAGTCCCTGATGTGGTCCCCGTCGTTGATCTCACCCAGTGTCCCAAGCACCGACAGTA
CTCCTAGCGCGAAGCCAGGGGCAGCCGAGGCACCCAGTGTGAAGTCAGCCATCCCCCACCTGCCCCGGCT
GCCCTCAGAGCCCCCTGCTCCCTCTCCCGGGCCCTCAGAGGCCCTAAGTGCTGTCTCCCTCCAGGCATCC
TCTGCTGATGGCTCCCCTGACTTCCCCATTGTAGCCATGCTTCGAGCCCCCAAACTGTGGCTTCTGCCAC
GCTCTACACTTGTCCCCAATATGACCCCAGTCCCACTGTCCCCAGCTTCTCCACTTCCCTCCTGGGTCCC
AGAAGAACAGGCTGTCAGGCCTGTCAGCCTTGGAGCGGAAGACCTCGAAACCCCATTTCAGACCACCATA
GCTGCCCCAGTTGAAGCCAGCCACAGATCCCCTGATGCAGATTCTATAGAAATCGAGGGGACCAGCTCCA
TGCGGGCTACAAAGCACCCCATCTCTGGCCCATGGGCTTCTTTGGACTCTAGTAATGTGACGATGAATCC
TGTCCCTTCTGATGCTGGCATCCTAGGGACTGAGTCTGGGGTCTTGGACTTACCAGGGAGTCCCACATCA
GGCGGACAGGCCACTGTGGAGAAGGTGCTGGCCACCTGGCTACCACTGCCTGGCCAAGGACTAGACCCTG
GCTCCCAGTCCACACCCATGGAAGCCCATGGGGTTGCCGTGAGCATGGAACCTACAGTGGCTTTGGAGGG
AGGTGCCACCGAGGGCCCTATGGAGGCCACCAGGGAGGTGGTCCCCAGCACTGCTGATGCCACTTGGGAG
TCTGAATCCAGAAGTGCCATTTCTAGCACCCACATAGCTGTGACCATGGCTAGGGCTCAGGGCATGCCCA
CACTGACCTCTACAAGCTCCGAAGGCCACCCAGAGCCTAAGGGCCAGATGGTGGCCCAGGAGTCACTGGA
GCCTCTCAACACTCTGCCTTCGCACCCCTGGTCATCCCTGGTGGTCCCCATGGATGAAGTGGCCTCAGTT
TCCTCAGGGGAACCCACAGGACTGTGGGACATACCCAGCACTCTGATACCTGTATCCTTGGGCTTGGATG
AATCAGACCTGAATGTTGTGGCTGAGAGTCCAAGCGTGAAGGGCTTCTGGGAAGAGGTGGCCAGCGGGCA
GGAAGATCCCACAGATCCCTGCGAGAACAACCCCTGCCTGCATGGGGGCACCTGCCACACCAACGGCACC
GTGTATGGCTGTAGTTGTGACCAGGGCTATGCTGGGGAGAATTGTGAAATTGACATTGATGACTGCTTAT
GCAGCCCTTGCGAGAATGGGGGCACCTGCATCGACGAGGTGAATGGTTTCATCTGCCTCTGCCTCCCCAG
CTATGGGGGCAGCCTGTGTGAGAAGGACACAGAAGGCTGCGACCGTGGCTGGCACAAATTCCAGGGACAC
TGCTACCGGTACTTTGCCCATCGCCGGGCCTGGGAGGATGCAGAGAGAGATTGCAGGCGCCGAGCTGGTC
ACCTGACAAGCGTCCATTCGCCAGAAGAGCACAAGTTCATTAACAGTTTTGGACATGAGAATTCGTGGAT
CGGCCTGAATGACCGGACAGTAGAGAGGGACTTCCAGTGGACAGACAACACAGGACTGCAATATGAGAAC
TGGAGGGAGAAGCAGCCGGATAATTTCTTCGCAGGTGGGGAGGATTGTGTGGTGATGGTGGCACATGAGA
GTGGGCGCTGGAATGATGTCCCCTGCAACTACAACCTACCCTATGTCTGCAAGAAGGGTACAGTGCTGTG
TGGTCCCCCTCCAGCAGTGGAGAATGCCTCTCTTGTTGGTGTGCGCAAGATCAAGTACAATGTCCATGCC
ACTGTGCGATACCAGTGTGATGAAGGATTCTCCCAGCACCGAGTGGCCACTATCCGCTGCCGTAACAATG
GGAAGTGGGACCGGCCTCAGATCATGTGCATCAAACCCAGGCGGTCACATCGGATGCGTAGACACCACCA
CCATCCACACCGACATCACAAGCCTCGCAAGGAGCACAGAAAACACAAGAGACACCCAGCGGAAGACTGG
GAGAAAGACGAAGGGGATTTCTGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211874 representing BC065118
Red=Cloning site Green=Tags(s)

MGAGSVWASGLLLLWLLLLVAGDQDTQDTTATEKGLRMLKSGSGPVRAALAELVALPCFFTLQPRLSSLR
DIPRIKWTKVQTASGQRQDLPILVAKDNVVRVAKGWQGRVSLPAYPRHRANATLLLGPLRASDSGLYRCQ
VVKGIEDEQDLVTLEVTGVVFHYRAARDRYALTFAEAQEACRLSSATIAAPRHLQAAFEDGFDNCDAGWL
SDRTVRYPITQSRPGCYGDRSSLPGVRSYGRRDPQELYDVYCFARELGGEVFYVGPARRLTLAGARAQCQ
RQGAALASVGQLHLAWHEGLDQCDPGWLADGSVRYPIQTPRRRCGGPAPGVRTVYRFANRTGFPAPGARF
DAYCFRAHHHTAQHGDSEIPSSGDEGEIVSAEGPPGRELKPSLGEQEVIAPDFQEPLMSSGEGEPPDLTW
TQAPEETLGSTPGGPTLASWPSSEKWLFTGAPSSMGVSSPSDMGVDMEATTPLGTQVAPTPTMRRGRFKG
LNGRHFQQQGPEDQLPEVAEPSAQPPTLGATANHMRPSAATEASESDQSHSPWAILTNEVDEPGAGSLGS
RSLPESLMWSPSLISPSVPSTDSTPSAKPGAAEAPSVKSAIPHLPRLPSEPPAPSPGPSEALSAVSLQAS
SADGSPDFPIVAMLRAPKLWLLPRSTLVPNMTPVPLSPASPLPSWVPEEQAVRPVSLGAEDLETPFQTTI
AAPVEASHRSPDADSIEIEGTSSMRATKHPISGPWASLDSSNVTMNPVPSDAGILGTESGVLDLPGSPTS
GGQATVEKVLATWLPLPGQGLDPGSQSTPMEAHGVAVSMEPTVALEGGATEGPMEATREVVPSTADATWE
SESRSAISSTHIAVTMARAQGMPTLTSTSSEGHPEPKGQMVAQESLEPLNTLPSHPWSSLVVPMDEVASV
SSGEPTGLWDIPSTLIPVSLGLDESDLNVVAESPSVKGFWEEVASGQEDPTDPCENNPCLHGGTCHTNGT
VYGCSCDQGYAGENCEIDIDDCLCSPCENGGTCIDEVNGFICLCLPSYGGSLCEKDTEGCDRGWHKFQGH
CYRYFAHRRAWEDAERDCRRRAGHLTSVHSPEEHKFINSFGHENSWIGLNDRTVERDFQWTDNTGLQYEN
WREKQPDNFFAGGEDCVVMVAHESGRWNDVPCNYNLPYVCKKGTVLCGPPPAVENASLVGVRKIKYNVHA
TVRYQCDEGFSQHRVATIRCRNNGKWDRPQIMCIKPRRSHRMRRHHHHPHRHHKPRKEHRKHKRHPAEDW
EKDEGDFC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC065118
ORF Size 3806 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC065118, AAH65118
RefSeq Size 7147 bp
RefSeq ORF 3806 bp
Locus ID 13004
Gene Summary May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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