Sec24b (BC046776) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI605202; SEC24
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211855 representing BC046776 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCCCCGCCGGGTCCCCTCACCCGGCCGCCGGCGCCCGGATGCCGCCCAAGCTCGGCGGAGCTG TCTCCGGCCTGGCACCGCCGCAGCAGAACGGTCCAGCACAGAGTCCAATGCAAGTTCCATCTGGGTATGG GCTGCCTCATCAAAACTATATGGTCCCTTCAGGACATTACTCTCAAGGACCTGGGAAAATGACCTCCTTG CCATTGGATAGCCAGTGTGATAGTTACTACTCTCGTCCATATACAGTACCAACACAAAATTCGGGGACTC CCAGCTCAGCAAACCAACCAGGAGCACAGCAGATGTATGGCAGAGGTCCTTCCGCTCCTCACATGGGGGG CTCCATGCCCGGATCTTTCCAAGGAATTCCAGCATCAGCATCCCATTCCTATTCGAGTGCCTCCCAGCCC TACTCCTCTCTTGGGAATCGCTACAGTAGTCCTACCACGTACTCTGCCAATGCTTCTGTTGCTTCTCAGG GATATCCCTCTACCTGTAGTCATTATCCCATCTCAACTGTGTCTAATGTGGTGTATCCTAATGTTTCCTA TCCCTCGCTGCCTGCCAGTGAGCCATATGGGCAGATGTTTACCTCACAGAGTGCTCCTCCACCTGCCAGG CCAGTTAAAGAGTCATACTCTGGCCCCAGCACAGCTCTTACCTACCCCTCGAGACCTCCACCTCCACCTT CTCAGCACCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGAGTCATTCAGGATATAG TTCTCTACCATGGTCAGGTCCAGCCCTTCCACCAGCCCAAGACAGCCTCATCCGAAACCAAATGGGATCC TTGGCTACAGCGAACAGCCACCCAACTAATAATGAAAATGTCCAACCTCCCAAGTCAAGCTCCGTAGTAT CCACAGTTTTGCCAGGACCCTCATCAACAAGAATGCCACCTGCTCCAAGTCACCCAGTTGGGCCTGTGCC CTCCGCTCCACCCCCCCCAGAGCAGATGCAGACTAAAGGCATGCAGTATGGTGACTATGGTAATAATCAA GCTAGCTCCACAGCGACTCCCTTGTCATCAGCTTCCGATGATGAGGAGGAGCAGGAGGAGGATGAGGAAG CAGGTGTTGACAGCTCTTCCACCACCAGCAGTGCTTCGCCCTTGCCCAACAGTTACGATGCCTTGGAAGG AGGCAGCTACCCAGATATGCATTCTTCTTCAGCAAGCAGCCCTGTTCCTGACCATGCCCTGGAACCCAGT CCCACCCTCGCCCAGGCTCTGTCTGCAGCCCCCACTCCCCCTGCAGCTCAGCCAGCCAAGGTGGCAAAGC CTTTTGGCTATGGCTACCCAGCTCTGCAGCCTGCCTATCAGAACGCAGCACCACCACCCATGCCCGCAGC ACATCCCAGCGGCCCAGCGTACACTGGATATCCTCAGCATTACCCAGGTGTGAACCAGCTGTCCTCAGGT CTCGGAGGACTAAGCCTGCAGAGTTCTCCACAGCCAGAAAGCCTAAGACCTGTCAACCTCACCCAGGAGA AAAATATTTTACCTCCAACTCCTATTTGGGCTCCTGTACCTAACCTGAGTGCAGAACTCAGCAAGTTAAA CTGTAGTCCAGACTCGTTTCGATGTACCTTGACCAGTATCCCCCAGACACAGGCTTTACTGAATAAAGCT AAGCTTCCTTTAGGATTGCTGTTGCATCCCTTCAGAGACCTGACGCAATTGCCAGTGATAACCTCAAACA CCATCGTGAGGTGCCGGTCCTGTCGGACGTACATCAACCCTTTTGTGTCCTTCATTGATCAGCGAAGATG GAAATGCAACCTGTGTTATAGAGTTAATGATGTTCCAGAAGAGTTTTTGTACAACCCCCTGACTCGGTCT TACGGAGAGCCTCATAAACGGCCAGAAGTCCAGAATTCTACTGTGGAGTTCATCGCATCCTCAGACTACA TGCTCCGGCCTCCTCAGCCCGCGGTCTACTTGTTTGTTTTAGATGTGTCTCATAACGCAGTGGAAGCTGG GTATTTGACAGTTTTATGCCAGTCCTTATTAGAAAACTTAGACAAGCTTCCTGGAGATTCACGAACAAGA ATAGGATTCATGACTTTTGACAGCACCATTCACTTCTACAATCTACAAGAAGGGTTATCCCAGCCGCAGA TGTTGATTGTATCTGATATAGACGATGTTTTTCTGCCCACGCCGGATAGTCTGCTTGTGAATCTGTATGA AAGCAAAGAGCTTATAAAAGACTTACTGAATGCATTACCAAGTATGTTCATCAATACCAGAGAAACACAC AGTGCCCTTGGACCTGCACTTCAGGCTGCCTTCAAACTCATGTCTCCCACGGGCGGCCGCGTGTCTGTAT TTCAGACACAGTTACCTTCCTTGGGGGCAGGACTTCTGCAGTCCAGAGAAGATCCGAATCAGAGGTCGAG TACCAAGGTGGTGCACCATCTTGGCCCTGCAACCGACTTTTATAAGAAGCTCGCTTTAGATTGCTCAGGG CAGCAAACTGCAGTGGATTTGTTCCTTCTAAGTTCACAGTATTCTGACCTTGCTTCTCTGGCTTGCATGT CCAAGTACTCTGCAGGGTGCATATTCTACTATCCATCTTTCCACTCTACCCACAATCCTTCGCAAGCAGA AAAGTTACAAAAAGACCTAAAACGGTACCTCACGAGGAAGATTGGATTTGAAGCTGTTATGAGAATAAGA TGCACTAAAGGGCTCTCAATGCACACTTTCCACGGGAACTTCTTTGTCCGCTCCACCGACTTACTGTCTC TTGCCAACATCAATCCTGATGCTGGGTTTGCAGTACAGTTGTCAATCGAAGAGAGCCTGACAGATACTGC CTTAGTGTGCTTTCAGACAGCCCTGCTGTACACATCTAGCAAAGGCGAGCGGAGAATTCGAGTGCATACA CTGTGCTTGCCAGTCGTAAGTTCCCTCGCTGACGTTTATGCAGGAGTGGATGTGCAAGCCGCTGTCTGCC TTCTCGCCAACATGGCTGTGGACCGCTCAGTGTCATCGAGCCTGTCGGATGCCAGGGATGCCCTGGTGAA CGCTGTGGTGGATCCCCTGTCTGCCTACAGCTCCGCCGTGGCAAGTGTGCCCCGCTCCACCCTGACAGCA CCAAGCTCACTTAAGCTGCTCCCACTCTATGTTTTGGCACTTCTTAAACAGAAAGCATTTAGAACAGGGA CAAGCACACGCCTGGATGACCGTGTCTATGCCATGTGCCAGATGAAGTCGCAGCCGCTTGTGCATCTCAT GAAAATGATTCATCCCAACTTATACAGGATAGACAGGCTGACAGACGAGGGCGCCATCCACGTGAATGAC AGGGTAGTACCTCAGCCACCGCTTCAGAAGCTGTCTGCAGAGAAGCTGACCCGAGAAGGCGCTTTCCTCA TGGACTGCGGCTCAGTTTTCTACATTTGGGTTGGCAAAGGCTGTGACAGTAACTTCATAGAGAACGTGCT TGGATACCCTGACTTTGCATCAATACCACAGAAAATGACACATCTTCCAGAGCTAGACACACTTCCATCA GAAAGAACTAGATCCTTTGTGACTTGGCTCAGGGACAGCCGACCGTTAAGCCCTGTCCTTCACTTGGTAA AAGATGAGAGCCCTGCCAAAACAGACTTTTTCCAGCATTTGCTTGAAGACCGCACGGAAGCCGCACTCTC CTACTATGAGTTTCTGATTCACATCCAACAGCAGGTTTGTAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211855 representing BC046776 Red=Cloning site Green=Tags(s) MSAPAGSPHPAAGARMPPKLGGAVSGLAPPQQNGPAQSPMQVPSGYGLPHQNYMVPSGHYSQGPGKMTSL PLDSQCDSYYSRPYTVPTQNSGTPSSANQPGAQQMYGRGPSAPHMGGSMPGSFQGIPASASHSYSSASQP YSSLGNRYSSPTTYSANASVASQGYPSTCSHYPISTVSNVVYPNVSYPSLPASEPYGQMFTSQSAPPPAR PVKESYSGPSTALTYPSRPPPPPSQHQQQQQQQQQQQQQQQSHSGYSSLPWSGPALPPAQDSLIRNQMGS LATANSHPTNNENVQPPKSSSVVSTVLPGPSSTRMPPAPSHPVGPVPSAPPPPEQMQTKGMQYGDYGNNQ ASSTATPLSSASDDEEEQEEDEEAGVDSSSTTSSASPLPNSYDALEGGSYPDMHSSSASSPVPDHALEPS PTLAQALSAAPTPPAAQPAKVAKPFGYGYPALQPAYQNAAPPPMPAAHPSGPAYTGYPQHYPGVNQLSSG LGGLSLQSSPQPESLRPVNLTQEKNILPPTPIWAPVPNLSAELSKLNCSPDSFRCTLTSIPQTQALLNKA KLPLGLLLHPFRDLTQLPVITSNTIVRCRSCRTYINPFVSFIDQRRWKCNLCYRVNDVPEEFLYNPLTRS YGEPHKRPEVQNSTVEFIASSDYMLRPPQPAVYLFVLDVSHNAVEAGYLTVLCQSLLENLDKLPGDSRTR IGFMTFDSTIHFYNLQEGLSQPQMLIVSDIDDVFLPTPDSLLVNLYESKELIKDLLNALPSMFINTRETH SALGPALQAAFKLMSPTGGRVSVFQTQLPSLGAGLLQSREDPNQRSSTKVVHHLGPATDFYKKLALDCSG QQTAVDLFLLSSQYSDLASLACMSKYSAGCIFYYPSFHSTHNPSQAEKLQKDLKRYLTRKIGFEAVMRIR CTKGLSMHTFHGNFFVRSTDLLSLANINPDAGFAVQLSIEESLTDTALVCFQTALLYTSSKGERRIRVHT LCLPVVSSLADVYAGVDVQAAVCLLANMAVDRSVSSSLSDARDALVNAVVDPLSAYSSAVASVPRSTLTA PSSLKLLPLYVLALLKQKAFRTGTSTRLDDRVYAMCQMKSQPLVHLMKMIHPNLYRIDRLTDEGAIHVND RVVPQPPLQKLSAEKLTREGAFLMDCGSVFYIWVGKGCDSNFIENVLGYPDFASIPQKMTHLPELDTLPS ERTRSFVTWLRDSRPLSPVLHLVKDESPAKTDFFQHLLEDRTEAALSYYEFLIHIQQQVCK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC046776
ORF Size	3755 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC046776, AAH46776
RefSeq Size	4719 bp
RefSeq ORF	3755 bp
Locus ID	99683

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