Sec24b (BC046776) Mouse Tagged ORF Clone

CAT#: MG211855

  • TrueORF®

Sec24b (tGFP-tagged) - Mouse SEC24 related gene family, member B (S. cerevisiae) (cDNA clone MGC:61181 IMAGE:5706007)



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Mouse Protein / Lysate Request

CNY 10,550.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI605202; SEC24
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211855 representing BC046776
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCCCCCGCCGGGTCCCCTCACCCGGCCGCCGGCGCCCGGATGCCGCCCAAGCTCGGCGGAGCTG
TCTCCGGCCTGGCACCGCCGCAGCAGAACGGTCCAGCACAGAGTCCAATGCAAGTTCCATCTGGGTATGG
GCTGCCTCATCAAAACTATATGGTCCCTTCAGGACATTACTCTCAAGGACCTGGGAAAATGACCTCCTTG
CCATTGGATAGCCAGTGTGATAGTTACTACTCTCGTCCATATACAGTACCAACACAAAATTCGGGGACTC
CCAGCTCAGCAAACCAACCAGGAGCACAGCAGATGTATGGCAGAGGTCCTTCCGCTCCTCACATGGGGGG
CTCCATGCCCGGATCTTTCCAAGGAATTCCAGCATCAGCATCCCATTCCTATTCGAGTGCCTCCCAGCCC
TACTCCTCTCTTGGGAATCGCTACAGTAGTCCTACCACGTACTCTGCCAATGCTTCTGTTGCTTCTCAGG
GATATCCCTCTACCTGTAGTCATTATCCCATCTCAACTGTGTCTAATGTGGTGTATCCTAATGTTTCCTA
TCCCTCGCTGCCTGCCAGTGAGCCATATGGGCAGATGTTTACCTCACAGAGTGCTCCTCCACCTGCCAGG
CCAGTTAAAGAGTCATACTCTGGCCCCAGCACAGCTCTTACCTACCCCTCGAGACCTCCACCTCCACCTT
CTCAGCACCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGAGTCATTCAGGATATAG
TTCTCTACCATGGTCAGGTCCAGCCCTTCCACCAGCCCAAGACAGCCTCATCCGAAACCAAATGGGATCC
TTGGCTACAGCGAACAGCCACCCAACTAATAATGAAAATGTCCAACCTCCCAAGTCAAGCTCCGTAGTAT
CCACAGTTTTGCCAGGACCCTCATCAACAAGAATGCCACCTGCTCCAAGTCACCCAGTTGGGCCTGTGCC
CTCCGCTCCACCCCCCCCAGAGCAGATGCAGACTAAAGGCATGCAGTATGGTGACTATGGTAATAATCAA
GCTAGCTCCACAGCGACTCCCTTGTCATCAGCTTCCGATGATGAGGAGGAGCAGGAGGAGGATGAGGAAG
CAGGTGTTGACAGCTCTTCCACCACCAGCAGTGCTTCGCCCTTGCCCAACAGTTACGATGCCTTGGAAGG
AGGCAGCTACCCAGATATGCATTCTTCTTCAGCAAGCAGCCCTGTTCCTGACCATGCCCTGGAACCCAGT
CCCACCCTCGCCCAGGCTCTGTCTGCAGCCCCCACTCCCCCTGCAGCTCAGCCAGCCAAGGTGGCAAAGC
CTTTTGGCTATGGCTACCCAGCTCTGCAGCCTGCCTATCAGAACGCAGCACCACCACCCATGCCCGCAGC
ACATCCCAGCGGCCCAGCGTACACTGGATATCCTCAGCATTACCCAGGTGTGAACCAGCTGTCCTCAGGT
CTCGGAGGACTAAGCCTGCAGAGTTCTCCACAGCCAGAAAGCCTAAGACCTGTCAACCTCACCCAGGAGA
AAAATATTTTACCTCCAACTCCTATTTGGGCTCCTGTACCTAACCTGAGTGCAGAACTCAGCAAGTTAAA
CTGTAGTCCAGACTCGTTTCGATGTACCTTGACCAGTATCCCCCAGACACAGGCTTTACTGAATAAAGCT
AAGCTTCCTTTAGGATTGCTGTTGCATCCCTTCAGAGACCTGACGCAATTGCCAGTGATAACCTCAAACA
CCATCGTGAGGTGCCGGTCCTGTCGGACGTACATCAACCCTTTTGTGTCCTTCATTGATCAGCGAAGATG
GAAATGCAACCTGTGTTATAGAGTTAATGATGTTCCAGAAGAGTTTTTGTACAACCCCCTGACTCGGTCT
TACGGAGAGCCTCATAAACGGCCAGAAGTCCAGAATTCTACTGTGGAGTTCATCGCATCCTCAGACTACA
TGCTCCGGCCTCCTCAGCCCGCGGTCTACTTGTTTGTTTTAGATGTGTCTCATAACGCAGTGGAAGCTGG
GTATTTGACAGTTTTATGCCAGTCCTTATTAGAAAACTTAGACAAGCTTCCTGGAGATTCACGAACAAGA
ATAGGATTCATGACTTTTGACAGCACCATTCACTTCTACAATCTACAAGAAGGGTTATCCCAGCCGCAGA
TGTTGATTGTATCTGATATAGACGATGTTTTTCTGCCCACGCCGGATAGTCTGCTTGTGAATCTGTATGA
AAGCAAAGAGCTTATAAAAGACTTACTGAATGCATTACCAAGTATGTTCATCAATACCAGAGAAACACAC
AGTGCCCTTGGACCTGCACTTCAGGCTGCCTTCAAACTCATGTCTCCCACGGGCGGCCGCGTGTCTGTAT
TTCAGACACAGTTACCTTCCTTGGGGGCAGGACTTCTGCAGTCCAGAGAAGATCCGAATCAGAGGTCGAG
TACCAAGGTGGTGCACCATCTTGGCCCTGCAACCGACTTTTATAAGAAGCTCGCTTTAGATTGCTCAGGG
CAGCAAACTGCAGTGGATTTGTTCCTTCTAAGTTCACAGTATTCTGACCTTGCTTCTCTGGCTTGCATGT
CCAAGTACTCTGCAGGGTGCATATTCTACTATCCATCTTTCCACTCTACCCACAATCCTTCGCAAGCAGA
AAAGTTACAAAAAGACCTAAAACGGTACCTCACGAGGAAGATTGGATTTGAAGCTGTTATGAGAATAAGA
TGCACTAAAGGGCTCTCAATGCACACTTTCCACGGGAACTTCTTTGTCCGCTCCACCGACTTACTGTCTC
TTGCCAACATCAATCCTGATGCTGGGTTTGCAGTACAGTTGTCAATCGAAGAGAGCCTGACAGATACTGC
CTTAGTGTGCTTTCAGACAGCCCTGCTGTACACATCTAGCAAAGGCGAGCGGAGAATTCGAGTGCATACA
CTGTGCTTGCCAGTCGTAAGTTCCCTCGCTGACGTTTATGCAGGAGTGGATGTGCAAGCCGCTGTCTGCC
TTCTCGCCAACATGGCTGTGGACCGCTCAGTGTCATCGAGCCTGTCGGATGCCAGGGATGCCCTGGTGAA
CGCTGTGGTGGATCCCCTGTCTGCCTACAGCTCCGCCGTGGCAAGTGTGCCCCGCTCCACCCTGACAGCA
CCAAGCTCACTTAAGCTGCTCCCACTCTATGTTTTGGCACTTCTTAAACAGAAAGCATTTAGAACAGGGA
CAAGCACACGCCTGGATGACCGTGTCTATGCCATGTGCCAGATGAAGTCGCAGCCGCTTGTGCATCTCAT
GAAAATGATTCATCCCAACTTATACAGGATAGACAGGCTGACAGACGAGGGCGCCATCCACGTGAATGAC
AGGGTAGTACCTCAGCCACCGCTTCAGAAGCTGTCTGCAGAGAAGCTGACCCGAGAAGGCGCTTTCCTCA
TGGACTGCGGCTCAGTTTTCTACATTTGGGTTGGCAAAGGCTGTGACAGTAACTTCATAGAGAACGTGCT
TGGATACCCTGACTTTGCATCAATACCACAGAAAATGACACATCTTCCAGAGCTAGACACACTTCCATCA
GAAAGAACTAGATCCTTTGTGACTTGGCTCAGGGACAGCCGACCGTTAAGCCCTGTCCTTCACTTGGTAA
AAGATGAGAGCCCTGCCAAAACAGACTTTTTCCAGCATTTGCTTGAAGACCGCACGGAAGCCGCACTCTC
CTACTATGAGTTTCTGATTCACATCCAACAGCAGGTTTGTAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211855 representing BC046776
Red=Cloning site Green=Tags(s)

MSAPAGSPHPAAGARMPPKLGGAVSGLAPPQQNGPAQSPMQVPSGYGLPHQNYMVPSGHYSQGPGKMTSL
PLDSQCDSYYSRPYTVPTQNSGTPSSANQPGAQQMYGRGPSAPHMGGSMPGSFQGIPASASHSYSSASQP
YSSLGNRYSSPTTYSANASVASQGYPSTCSHYPISTVSNVVYPNVSYPSLPASEPYGQMFTSQSAPPPAR
PVKESYSGPSTALTYPSRPPPPPSQHQQQQQQQQQQQQQQQSHSGYSSLPWSGPALPPAQDSLIRNQMGS
LATANSHPTNNENVQPPKSSSVVSTVLPGPSSTRMPPAPSHPVGPVPSAPPPPEQMQTKGMQYGDYGNNQ
ASSTATPLSSASDDEEEQEEDEEAGVDSSSTTSSASPLPNSYDALEGGSYPDMHSSSASSPVPDHALEPS
PTLAQALSAAPTPPAAQPAKVAKPFGYGYPALQPAYQNAAPPPMPAAHPSGPAYTGYPQHYPGVNQLSSG
LGGLSLQSSPQPESLRPVNLTQEKNILPPTPIWAPVPNLSAELSKLNCSPDSFRCTLTSIPQTQALLNKA
KLPLGLLLHPFRDLTQLPVITSNTIVRCRSCRTYINPFVSFIDQRRWKCNLCYRVNDVPEEFLYNPLTRS
YGEPHKRPEVQNSTVEFIASSDYMLRPPQPAVYLFVLDVSHNAVEAGYLTVLCQSLLENLDKLPGDSRTR
IGFMTFDSTIHFYNLQEGLSQPQMLIVSDIDDVFLPTPDSLLVNLYESKELIKDLLNALPSMFINTRETH
SALGPALQAAFKLMSPTGGRVSVFQTQLPSLGAGLLQSREDPNQRSSTKVVHHLGPATDFYKKLALDCSG
QQTAVDLFLLSSQYSDLASLACMSKYSAGCIFYYPSFHSTHNPSQAEKLQKDLKRYLTRKIGFEAVMRIR
CTKGLSMHTFHGNFFVRSTDLLSLANINPDAGFAVQLSIEESLTDTALVCFQTALLYTSSKGERRIRVHT
LCLPVVSSLADVYAGVDVQAAVCLLANMAVDRSVSSSLSDARDALVNAVVDPLSAYSSAVASVPRSTLTA
PSSLKLLPLYVLALLKQKAFRTGTSTRLDDRVYAMCQMKSQPLVHLMKMIHPNLYRIDRLTDEGAIHVND
RVVPQPPLQKLSAEKLTREGAFLMDCGSVFYIWVGKGCDSNFIENVLGYPDFASIPQKMTHLPELDTLPS
ERTRSFVTWLRDSRPLSPVLHLVKDESPAKTDFFQHLLEDRTEAALSYYEFLIHIQQQVCK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC046776
ORF Size 3755 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC046776, AAH46776
RefSeq Size 4719 bp
RefSeq ORF 3755 bp
Locus ID 99683
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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