Tpp2 (BC058239) Mouse Tagged ORF Clone

CAT#: MG211854

  • TrueORF®

Tpp2 (tGFP-tagged) - Mouse tripeptidyl peptidase II (cDNA clone MGC:65300 IMAGE:6308147)

ORF Plasmid: DDK tGFP



  "BC058239" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,890.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms TPP-2; TppII
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211854 representing BC058239
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACCGCCGCGACCGAGGAGCCCTTTCCTTTCCACGGTCTTCTACCAAAGAAGGAGACCGGGGCCT
CCTCCTTCCTGTGCCGCTACCCGGAGTATGACGGGCGCGGGGTGCTCATCGCCGTCCTGGACACAGGGGT
CGATCCCGGGGCCCCGGGCATGCAGGTTACAACTGATGGAAAACCAAAAATCATTGATATCATTGATACA
ACAGGAAGTGGTGATGTAAATACTGCTACAGAAGTAGAACCAAAAGATGGTGAAATTATTGGTCTTTCTG
GAAGAGTGCTTAAGATTCCTGCAAACTGGACAAATCCTTTAGGAAAATATCATATTGGCATTAAAAATGG
TTATGACTTCTATCCAAAGGCTCTCAAGGAAAGGATACAGAAAGAACGGAAGGAAAAAATCTGGGATCCA
ATTCACAGAGTTGCACTTGCAGAAGCTTGTAGAAAACAAGAAGAATTTGATATTGCCAACAATGGCTCTT
CCCAAGCCAATAAACTAATCAAGGAAGAGTTGCAAAGTCAAGTGGAATTACTTAATTCTTTTGAGAAAAA
GTATAGTGATCCAGGCCCTGTATATGACTGCTTGGTGTGGCATGATGGTGAGACCTGGAGAGCCTGTGTT
GATTCAAATGAGAATGGGGACTTGAGTAAATGTGCCGTATTGAGAAACTACAAAGAGGCCCAAGAGTACA
GTTCTTTTGGCACAGCTGAGATGCTGAATTACTCTGTGAACATTTATGACGATGGGAACCTGCTCTCCAT
TGTGACCAGCGGAGGAGCTCATGGAACCCATGTAGCAAGTATAGCCGCAGGGCATTTTCCAGAAGAGCCT
GAACGGAATGGAGTTGCTCCTGGTGCTCAAATTCTATCCATTAAGATTGGTGATACACGGCTAAGCACTA
TGGAAACAGGCACAGGCCTCATCAGAGCTATGATAGAAGTTATAAATCATAAGTGTGATCTTGTCAACTA
CAGTTATGGAGAAGCAACTCATTGGCCAAATTCTGGGAGAATTTGTGAAGTAATTAATGAAGCAGTATGG
AAACATAATACAATTTATGTTTCAAGTGCTGGAAATAATGGTCCATGCCTTTCTACAGTGGGTTGTCCAG
GAGGAACTACATCCAGTGTGATAGGTGTTGGAGCTTATGTTTCCCCTGATATGATGGTTGCAGAGTATTC
ACTGAGAGAGAAACTGCCTGCAAATCAATATACATGGTCTTCTAGAGGCCCAAGTGCTGATGGAGCCCTC
GGTGTGAGCATCAGTGCACCAGGAGGTGCTATTGCTTCTGTGCCTAACTGGACATTGAGGGGGACTCAGC
TAATGAATGGGACATCAATGTCTTCCCCCAATGCCTGTGGTGGCATTGCCCTGGTACTTTCAGGGCTGAA
AGCAAATAATGTTGACTATACTGTACACTCAGTCAGAAGAGCTCTAGAAAATACTGCAATAAAAGCTGAC
AATATAGAAGTATTTGCCCAAGGACATGGAATTATTCAGGTTGACAAAGCTTATGACTACCTCATTCAAA
ATACATCATTTGCTAACAGATTAGGTTTTACAGTTACTGTTGGAAATAACCGTGGTATCTACCTCCGAGA
TCCTGTCCAGGTGGCTGCTCCTTCAGATCATGGTGTTGGCATTGAGCCTGTATTTCCAGAGAACACAGAA
AACTCTGAAAAAATATCCTTTCAGCTTCATTTAGCTTTAACTTCAAATTCATCTTGGGTTCAGTGTCCCA
GCCATTTGGAACTCATGAATCAGTGTCGGCACATAAACATACGTGTGGACCCCAGGGGCTTAAGAGAAGG
GTTACATTATACAGAGGTATGTGGCTATGATATAGCATCCCCCAATGCAGGTCCTTTATTCAGAGTTCCA
ATCACTGCAGTTATAGCAGCAAAAGTAAATGAGTCATCACATTATGATCTAGCCTTTACAGATGTACATT
TTAAACCTGGTCAGATTCGAAGACATTTTGTTGAGGTTCCTGAAGGGGCAACCTGGGCTGAAGTTACCGT
GTGTTCATGTTCTTCTGAGGTATCGGCAAAATTTGTTCTTCATGCAGTACAGCTTGTGAAGCAGAGAGCA
TATCGAAGTCATGAATTTTATAAGTTTTGTTCCCTTCCAGAAAAAGGAACACTTATTGAAGCTTTTCCTG
TTTTGGGCGGAAAAGCAATTGAATTTTGTATTGCTCGTTGGTGGGCAAGTCTTAGTGATGTCAATATTGA
TTATACCATATCATTCCATGGGATAGTGTGTACTGCACCACAGTTAAACATTCATGCATCTGAAGGAATC
AATCGTTTTGATGTTCAGTCCTCTTTAAAGTATGAAGATCTGGCTCCTTGCATAACTTTGAAGAGCTGGG
TGCAAACGCTACGCCCAGTAAATGCAAAAACCAGACCTTTAGGATCAAGAGATGTTTTGCCAAATAATCG
CCAGCTTTATGAGATGGTCCTTACATACAGCTTTCATCAGCCCAAGAGCGGAGAAGTAACACCTAGTTGT
CCACTCCTTTGTGAATTGTTATATGAGTCAGAATTTGACAGTCAGTTGTGGATTATTTTCGACCAGAACA
AAAGACAGATGGGCTCAGGCGATGCCTATCCACATCAGTATTCTCTGAAATTGGAGAAAGGAGATTATAC
AATTCGATTACAGATTCGTCATGAGCAAATCAGTGATTTGGATCGTCTCAAAGATCTTCCGTTTATTGTT
TCACATAGGTTGTCTAATACCTTGAGCTTAGATATTCATGAAAATCATAGCCTTGCACTTCTAGGAAAGA
AGAAATCAAGCAGCTTAACATTACCACCCAAATACAATCAGCCATTCTTTGTTACTTCCTTACCTGATGA
TAAAATACCTAAGGGGGCAGGACCCGGATGCTACCTTGCAGGCTCCTTGACATTGTCAAAGACTGAGCTT
GGAAAGAAAGCTGATGTGATCCCTGTTCATTACTATCTCATACCTCCACCAACAAAGATTAAGAATGGCA
GCAAAGATAAAGAAAAGGATTCAGAAAAAGAGAAAGACTTGAAAGAAGAGTTTACTGAAGCATTACGCGA
TCTCAAAATTCAGTGGATGACCAAGCTTGATTCTACTGACATTTACAATGAATTGAAAGAAACATATCCT
GCTTACCTTCCTTTGTATGTTGCACGTCTTCATCAACTGGATGCTGAAAAGGAACGAATGAAAAGACTTA
ATGAAATTGTTGATGCTGCCAATGCTGTTATTTCTCACATCGATCAAACCGCTCTTGCAGTTTACATTGC
AATGAAGACTGACCCCAGGCCTGATGCAGCTACTATAAAAAATGATATGGACAAGCAGAAATCCACCCTG
ATAGATGCCCTCTGCAGGAAAGGATGTGCCCTGGCAGATCACCTTCTTCATACACAGCCTCACGACGGGG
CAGCAGCTGGAGATGCTGAAGCAAAGGAAGAAGAAGGAGAAAGTACCATGGAATCTCTATCGGAAACCTA
TTGGGAAACTACAAAGTGGACAGATCTTTTTGACACTAAGGTTTTGATATTTGCATACAAGCATGCATTA
GTAAATAAGATGTATGGGAGAGGCCTTAAGTTTGCAACCAAACTCGTAGAAGAAAAACCAACAAAAGAAA
ACTGGAAAAATTGTATTCAACTCATGAAATTACTTGGATGGACCCACTGTGCATCTTTTACTGAAAACTG
GCTCCCCATCATGTATCCTCCTGATTATTGTGTATTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211854 representing BC058239
Red=Cloning site Green=Tags(s)

MATAATEEPFPFHGLLPKKETGASSFLCRYPEYDGRGVLIAVLDTGVDPGAPGMQVTTDGKPKIIDIIDT
TGSGDVNTATEVEPKDGEIIGLSGRVLKIPANWTNPLGKYHIGIKNGYDFYPKALKERIQKERKEKIWDP
IHRVALAEACRKQEEFDIANNGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGETWRACV
DSNENGDLSKCAVLRNYKEAQEYSSFGTAEMLNYSVNIYDDGNLLSIVTSGGAHGTHVASIAAGHFPEEP
ERNGVAPGAQILSIKIGDTRLSTMETGTGLIRAMIEVINHKCDLVNYSYGEATHWPNSGRICEVINEAVW
KHNTIYVSSAGNNGPCLSTVGCPGGTTSSVIGVGAYVSPDMMVAEYSLREKLPANQYTWSSRGPSADGAL
GVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKANNVDYTVHSVRRALENTAIKAD
NIEVFAQGHGIIQVDKAYDYLIQNTSFANRLGFTVTVGNNRGIYLRDPVQVAAPSDHGVGIEPVFPENTE
NSEKISFQLHLALTSNSSWVQCPSHLELMNQCRHINIRVDPRGLREGLHYTEVCGYDIASPNAGPLFRVP
ITAVIAAKVNESSHYDLAFTDVHFKPGQIRRHFVEVPEGATWAEVTVCSCSSEVSAKFVLHAVQLVKQRA
YRSHEFYKFCSLPEKGTLIEAFPVLGGKAIEFCIARWWASLSDVNIDYTISFHGIVCTAPQLNIHASEGI
NRFDVQSSLKYEDLAPCITLKSWVQTLRPVNAKTRPLGSRDVLPNNRQLYEMVLTYSFHQPKSGEVTPSC
PLLCELLYESEFDSQLWIIFDQNKRQMGSGDAYPHQYSLKLEKGDYTIRLQIRHEQISDLDRLKDLPFIV
SHRLSNTLSLDIHENHSLALLGKKKSSSLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGSLTLSKTEL
GKKADVIPVHYYLIPPPTKIKNGSKDKEKDSEKEKDLKEEFTEALRDLKIQWMTKLDSTDIYNELKETYP
AYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKNDMDKQKSTL
IDALCRKGCALADHLLHTQPHDGAAAGDAEAKEEEGESTMESLSETYWETTKWTDLFDTKVLIFAYKHAL
VNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC058239
ORF Size 3747 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC058239.1
RefSeq Size 3960 bp
RefSeq ORF 3749 bp
Locus ID 22019
Gene Summary Component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. May be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited (By similarity). Stimulates adipogenesis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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