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Phka2 (NM_172783) Mouse Tagged ORF Clone
CAT＃: MG211836
- TrueORF®
Phka2 (tGFP-tagged) - Mouse phosphorylase kinase alpha 2 (Phka2)
"NM_172783" in other vectors (5)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6330505C01Rik; D330034O08; Phk |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211836 representing NM_172783
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTTGGGCGAGATGCGGAGCAGGAGCAATTCTGGGGTCCGCCTGGACGGGTACGCGAGGCTGGTGC AGCAAACCATCCTGTGTTACCAGAACCCAGTCACTGGGCTGTTATCAGCCAGCCATGACCAGAAGGATGC CTGGGTACGGGACAATATCTATAGCATATTGGCTGTTTGGGGCCTGGGCATGGCCTACCGCAAGAATGCT GACCGTGATGAGGATAAAGCCAAGGCCTATGAGTTGGAACAGAACGTAGTGAAGCTGATGCGAGGTCTTC TCCAGTGTATGATGAGGCAGGTGGACAAAGTGGAGAAGTTCAAGCACACTCAGAGTACCAAGGACAGTCT ACATGCCAAGTACAACACTGCCACATGTAGCACTGTGGTGGGTGATGACCAGTGGGGCCACCTCCAGGTG GATGCCACCTCTCTCTTCCTCCTGTTCCTGGCCCAGATGACAGCCTCAGGTTTGCGTATTATTTTTACCC TTGACGAAGTGGCCTTCATACAGAATCTTGTCTTCTACATAGAAGCTGCATATAAAGTTGCTGATTATGG GATGTGGGAGCGTGGAGATAAAACCAATCAGGGCATTCCGGAACTGAATGCAAGCTCGGTGGGAGTGGCC AAGGCAGCACTTGAGGCCATTGATGAACTAGATCTTTTTGGAGCCCATGGAGGACGCAAATCGGTGATCC ACGTCCTACCTGATGAAGTTGAGCACTGCCAGTCAATTCTTTTCTCCATGTTGCCAAGAGCATCAACATC TAAAGAAATCGATGCTGGACTTCTTTCTATTATTTCTTTCCCGGCCTTTGCAGTAGAAGATGTGAACCTT GTGAATGTGACCAAAAATGAAATTATTTCCAAGCTTCAGGGGCGGTATGGATGCTGTCGCTTCCTTCGAG ATGGCTATAAAACCCCAAGAGAGGATCCACACCGATTGCATTATGACCCTGCCGAACTTAAGCTCTTTGA AAACATTGAATGTGAATGGCCTGTGTTCTGGACTTATTTAATCATAGATGGAATCTTCAATGGTGATGCT GTTCAGGTCCAAGAATACCGAGAAGCCTTGGAGGGAATATTAATCAGAGGCAAAGATGGGATCCACTTGG TGCCAGAACTCTATGCCATCCCACCAGACAAGGTGGATGAAGAGTATAAGAATCCACACACAGTAGACCG AGTTCCACTGGGAAAGCTGCCCCATCTTTGGGGACAGTCCTTGTACATCCTCAGCTCACTGCTAGCAGAG GGATTCCTTGCCACGGGTGAAATCGATCCCTTAAATAGAAGATTTTCTACTTCAGTCAAACCTGATGTTG TAGTGCAAGTTGCTGTTTTGGCAGAAAACAGTCACATTAAGGGGCTGTTGAAGGAGCATGGAATGACTGT CCAGAGCATTGCTGATGTGCATCCGATTCGAGTCCAGCCAGGCCGAATTCTTAGTCACATATATGCCAAA CTTGGACGAAATAAGAATATGAAATTGAGTGGTCGACCGTATCGGCACATTGGTGTCCTTGGCACCTCTA AACTCTATGTGATTAGGAATCACATCTTCACTTTTACACCCCAGTTCACTGACCAGCATCACTTCTACCT GGCCCTGGACAATGAGATGATTGTGGAGATGCTGAGGATCGAACTTGCCTATCTGTGCACCTGCTGGCGG ATGACCGGCCGACCCACTCTCACCTTCCCTGTCACGCATACCATGCTCACAAATGATGGATCAGACATTC ATCCTGCAGTTCTTTCTACAATTAGAAAACTAGAAGATGGCTATTTTGGAGGTGCTAGAGTAAAACTAGG AAATCTGGCAGAGTTCCTTACTACCTCATTCTACACACACCTGACCTTCCTGGATCCAGACTGCGATGAG AAGTTGTTTGGTGACATCACTGATAGGAGCTTTAGTCCTGACAGTGAGCCAGACCTGGGAGGATACCTGG AAGACAGCAGTCCTCAAGAAAGCCAAGATGAACTTGACCAGTATATCAGCCACCTTCTTCAAAGCACATC CTTGAAGTGTTACCTGCCCCCTCTTTGTAAGAAGTCAGAAGACAGCCATTTTTTCAGTGCTATTCACTCC ACTCGGGACATACTTTCTGTGATGGCCAAAGCGAAGGGTTTGGAAACTACATTTTTTCCCATGATTTTGC CAACTAAAGTTCTAAGTGGACACCGTAAGTCACTGAATCTTGTTGACTCCCCTCAGCCACTCCTAAAGAC GACTCCTGAATATGACTACCAGTGGCCCAGAGACGACCATGATGAAGTGGACTGTGAGAAGCTAGTTGGG CAACTGAAAGACTGCTCAAACCTACAGGACCAAGCAGACATTCTGTACATTCTTTATGTAATGAAGGGTC CCCGCTGGGATACCAATTTGTTTGGGCAGCATGGAGTCACTGTTCACAGTCTTCTCAGCGAGCTCTATGG AAAAGCTGGCCTAAACCAAGAATGGAGTTTGATTCGCTACATTTCAGGCCTGCTCAGGAAGAAAGTGGAG GTCCTGGCTGAGGCCTGTGCAGATCTGCTGTCCCACCAGAAGCAGCTTACAGTAGGACTGCCCCCTGAGC CCCGGGAGAAGACCATATCTACGCCTCTACCCCCAGAGGAGCTCACAGAACTCATATATGAAGCCAGTGG ACAGGACATCAGCATTGCTGTCCTCACACAGGAGATCGTGGTTTACCTGGCCATGTATGTCCGGGCCCAG CCTAGCCTCTTTGCAGAGATGCTCAGACTCCGGATTGGATTGATCATCCAGGTGATGGCCACAGAGCTGG CTCGGAGCCTGAACTGCTCAGGAAAAGAAGCTTCGGAGAGCTTGATGAACCTCAGCCCTTTCGACATGAA GAGCCTTCTGCATCATATCCTGAGTGGGAAGGAGTTCGGTGTGGAGAGAAGCGTGCGCCCAATACACTCC TCCATGTCCAGCCCTGCCATCTCCATCCATGAGGTGGGTCATACTGGAGCCACCAAAACTGAGCGGAGTG GCATCACCAGACTGAGGAGTGAGATGAAACAGATGAATAGGCGAGCTAGTGCTGATGAGCAGTTCTTTCC TTTGGGCCAGACCATGTCCAACAGTTTGCATTCCATCAAGTCTGTGAGGTCCAGCACCCCATCCTCCCCG ACAGGCACATCATCTACAGACTCTGGAGGACAGCACCTGGGCTGGGGAGAACAGCAGGGCCAGTGGCTGC GCCGGAGAAGGTTGGATGGGGCCATTAACAGGGTCCCCGTGGGATTCTACCAGAAAGTGTGGAAGATCCT TCAGAAGTGCCATGGCCTGTCCATCGATGGTTACGTCCTCCCATCCTCGACAACCCAAGAGATGACCCCT TGTGAGATCAAGTTTGCCGTCCATGTGGAGTCAGTGCTCAACCGTGTGTCCCAGCCTGAGTATCGGCAGC TACTGGTAGAAGCCATCATGGTACTGACTCTGCTCTCAGATACCGAAATGGACAGTATCGGGGGCATCAT CCATGTGGACCAGATAGTGCAGCTGGCCAATCAGCTGTTCCTGCAGGACCAGGTGTCATTTGGAACCACA GACATCCTGGAAAAAGACCAAGCCACAGGAATTTGCCACCTCTTTTATGACAGTGCTCCTAGTGGGGCTT ATGGTACAATGACCTACCTAACAAAAGCGGTCGCTTCTCATTTGCAGGAACTGCTGCCCAGTTCAGGCTG CCAGATGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211836 representing NM_172783
Red=Cloning site Green=Tags(s) MSLGEMRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHDQKDAWVRDNIYSILAVWGLGMAYRKNA DRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVEKFKHTQSTKDSLHAKYNTATCSTVVGDDQWGHLQV DATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGVA KAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNL VNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPHRLHYDPAELKLFENIECEWPVFWTYLIIDGIFNGDA VQVQEYREALEGILIRGKDGIHLVPELYAIPPDKVDEEYKNPHTVDRVPLGKLPHLWGQSLYILSSLLAE GFLATGEIDPLNRRFSTSVKPDVVVQVAVLAENSHIKGLLKEHGMTVQSIADVHPIRVQPGRILSHIYAK LGRNKNMKLSGRPYRHIGVLGTSKLYVIRNHIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWR MTGRPTLTFPVTHTMLTNDGSDIHPAVLSTIRKLEDGYFGGARVKLGNLAEFLTTSFYTHLTFLDPDCDE KLFGDITDRSFSPDSEPDLGGYLEDSSPQESQDELDQYISHLLQSTSLKCYLPPLCKKSEDSHFFSAIHS TRDILSVMAKAKGLETTFFPMILPTKVLSGHRKSLNLVDSPQPLLKTTPEYDYQWPRDDHDEVDCEKLVG QLKDCSNLQDQADILYILYVMKGPRWDTNLFGQHGVTVHSLLSELYGKAGLNQEWSLIRYISGLLRKKVE VLAEACADLLSHQKQLTVGLPPEPREKTISTPLPPEELTELIYEASGQDISIAVLTQEIVVYLAMYVRAQ PSLFAEMLRLRIGLIIQVMATELARSLNCSGKEASESLMNLSPFDMKSLLHHILSGKEFGVERSVRPIHS SMSSPAISIHEVGHTGATKTERSGITRLRSEMKQMNRRASADEQFFPLGQTMSNSLHSIKSVRSSTPSSP TGTSSTDSGGQHLGWGEQQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTP CEIKFAVHVESVLNRVSQPEYRQLLVEAIMVLTLLSDTEMDSIGGIIHVDQIVQLANQLFLQDQVSFGTT DILEKDQATGICHLFYDSAPSGAYGTMTYLTKAVASHLQELLPSSGCQMQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172783 |
| ORF Size | 4711 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172783.1 |
| RefSeq Size | 4459 bp |
| RefSeq ORF | 3708 bp |
| Locus ID | 110094 |
| UniProt ID | Q8BWJ3 |
| Gene Summary | Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202030 | Phka2 (untagged) - Mouse phosphorylase kinase alpha 2 (Phka2), transcript variant 1, (10ug) |
CNY 8,640.00 |
|
| MG220742 | Phka2 (tGFP-tagged) - Mouse phosphorylase kinase alpha 2 (Phka2) transcript variant 1, (10ug) |
CNY 10,550.00 |
|
| MR220742 | Phka2 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 2 (Phka2), transcript variant 1 |
CNY 8,600.00 |
|
| MR220742L3 | Lenti ORF clone of Phka2 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 2 (Phka2), transcript variant 1 |
CNY 11,500.00 |
|
| MR220742L4 | Lenti ORF clone of Phka2 (mGFP-tagged) - Mouse phosphorylase kinase alpha 2 (Phka2), transcript variant 1 |
CNY 11,500.00 |
