Trerf1 (BC059215) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	B830015H24, RAPA, Trep132, Trep-132
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211828 representing BC059215 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACCAGCAACTGTACAAGACCAATCACGTGGGCCACGGTGGTGAGAACCTCTTCTATCAACAGC CACCCCTTGGTGTCCACAGTGGGCTGGGCCACAGCTATGGGAATACAATCTCTGGGGCTGGGATGGACGC CCCGCAGGCCTCACCCATCTCGCCTCACTTCCCTCAAGATACTCGGGATGGTCTCGGCTTGCCTATTGGC TCCAAAAACCTTGGCCAGATGGATACCTCCAGGCAGGGAGGATGGGGAAGCCATGTAGGGCCTGGGAACC ACGTCCAGCTTCGTAGCAACTTGGCCAACTCAAACATGATGTGGGGGACGCCCACCCAGGTGGAGCCCGC TGATGGCTACCAATACACTTATTCCCAGGCCAGCGAGATCCGGACCCAGAAACTCACCAGTGGTGTTTTG CACAAGCTGGACTCTTTCACCCAGGTATTTGCCAACCAAAACCTGCGGATTCAGGTCAACAATATGGCCC AGGTGCTGCACACCCAATCAGCGGTGATGGATGGAGCCTCCGACAGTGCCCTCCGTCAACTGCTGTCTCA GAAGCCCGTGGAGCCCTCAGCATCAGCTATAGCTTCCCGCTACCAGCAGGTGCCCCAGCAGCCTCACCCT GGCTTCACGGGTGGACTGCCCAAACCAGCCCTTCCAGTCGGGCAGCACGCTCCCCAAGGGCACCTGTATT ATGACTACCAGCAGCCCCTGGCCCAGATGTCCATGCAAGGAGGACAGCCACTGCAAGCCCCTCAGGTGCT GTCCGGCCATATGCAACAATTGCAGCAGCACCAGTATTACCCACAGCCGCCGCCTCAGCAGCAGCAAGCC GGACTGCAGCGGATCTCTGTGCAGGAGATGCAGCAGCAGCAGCAGCCGCAGCAAATTCGCCCCTCACCAC CTCAGCAGCAGCAGCAGCTCCAGCTGCAGCAGCGGCAGAGTTCACTGCAGATACCTCAGTATTATCAGCC CCAACCCATGATGCAACACTTGCAAGAGCAGCAGCAGCCATCCATGCACCTGCAGCCACCCTCATACCAC AGGGACCCTCATCAGTATACCCCGGAGCAGGCACACGCTGTCCAGCTGATCCAGCTGGGCTCTATGCCCC AGTATTACTATCAGGAGCCTCAACAGGCCTATAGCCACCCCCTCTACCCGCAGAGCCACCTGTCCCAGCA CCAGCAGCGTGAAGACGGTCAGCTGAAGACATATTCTAGTGACAGACAGACCCCGGCTATGCTGAGCTCC CATGGGGACATGGGAACTTCTGATACAGGAGTGGCAGATCCAGCCAGCTCAGAAATGACTCGGGTCACTA GTACTCTTCCTCACCAACCGCTCCTGTCCCCCAGTGGGATCCACCTCAACAACATGGGGTCTCAGCATCA ACAGCCGCCATCTCCCAGTGCGATGTGGCCCCAGATGCACTTACCTGATGGGAGAGCCCAGTCCGGATCC CCGGAATCAAGCAGCGGCCAAACCAAAGGAGTGTTTGGGGAACAGTTTGATGCCAAGAACAAGCTGACAT GCTCCATCTGCCTGAAGGAGTTCAAAAGCCTGCCCGCCCTGAACGGCCACATGCGGTCCCACGGGGGGAT GAGGGCGTCCCCCAGCCTCAAACAGGAGGAAGGAGAGAAGGCCCCACCGCCTCAGCCTCAGCCCCAGCCT CAGCCCCAGCAGCCACTGCCACCTCCGCCTCCTCCGCCGCCGCCACCGCAGCTCCCTCCTGAGGCAGAGC GCCTCACGCCTATGGTCATGCCCGTGTCTGTCCCTGTCAAGCTAATCCCACCCAAGCCCAGCTCTCAGGG GTTCACCAACAGCGTCGCTGCCACCCCCGCGGCCAGAGACAAGCCAGCCAGCTCGATGTCGGACGACGAG ATGCCTGTGCTCGTGAGGATGACCCTCTCCCCCCCTCACTCCCCCCAAGGGGCTGCCCCCCGTGCGCCTG CTGAAATCCCCCGGAAACATCCACCTATCGCCGCCAAAGTTGAGGAGCCCCTCAAGAACCTACCAGAGAA GAAGAAATTCCGGCACCGGCCTGAGCCCCTCTTCATCCCACCACCGCCTTCGTCCTATACTCCCAACCCC ACCTCTTACTCGGGGGCCACCCTGTACCAGAGCCAGCTGCGCTCCCCGCGAATCCTCGGGGACCACCTGC TCCTGGACCCCGCCCACGAACTGCCCCCCTACACGCCGCCACCCATGCTGAGCCCTGTGCGCCAGGGCTC AGGGCTCTTCAGCAATGTCCTCATCTCTGGCCACGGCCCTGGAGTGCACCCCCAGCTGCCCCTCACTCCC CTCACGCCCACGCCACGTGTGCTGCTGTGCCGTTCCAGCAGCATCGATGGCAGCAATGTGACAGTCACCC CAGGACCTGGAGAGCAGACTGTGGATGTTGAACCACGCATCAACATTGGCTTAAGGTTCCAAGCAGAGAT CCCAGAACTGCAAGACGTCTCCGCCCTGGCTCAGGACACACACAGGGCCACACTGGTATGGAAGCCGTGG CCTGAGCTGGAAAACCAGGCCCTCCAGCAGCAAGTGGAGAATCTTCTGAATTTGTGTTGTTCCAGTGCGC TGCCAGGTGGAGGAACCAATTCGGAATTTGCTTTGCACTCTCTCTTTGAGGCCAAAGGTGACGTGATGGC TACCCTGGAAATGCTGCTGCTGCGGAAGCCAGTCAGGTTAAAATGTCATCCTTTAGCAAATTACCACTAC GCCGGTTCTGACAAGTGGACATCCCTAGAAAGAAAACTGTTTAATAAAGCATTGGCCACTTACAGCAAAG ACTTTATTTTTGTACAGAAGATGGTGAAGTCCAAGACAGTGGCTCAGTGCGTGGAGTACTACTACACTTG GAAGAAGATAATGCGACTAGGCCGGAAACACCGGACACGCCTGGCGGAAATCATTGACGACTGCATGACC AGCGAAGAGGAAGAGGAGGCCGAGGAGGAAGAAGAGGACCCAGAAGAAGATAGGAAATCCATAAAAGAAG AGGAGAGTGAAGTGGCCAAGTCTCCAGAGCCACCGCCTGCCCCTGCCCTGGCTCCCACTGAGGGGCCACC CATGCAGGCTGTTGGCCAACAACCATCAGGCAACTTCATTTGTGAAATGCCCAACTGTGGGGCTGTGTTC AGCTCCCGACAGGCACTGAACGGTCACGCCCGCATCCACGGCGGCACCAACCAGGTGGCTAAGACCCGAG GTGCCATCCCCTCTGGGAAGCAGAAGCCAGGTGGCACCCAGAGTGGGTACTGCTCAGTGAAGAGTTCACC ATCTCACAGTACCACCAGTGGAGAGACGGACCCCACCACCATCTTCCCCTGCAAGGAGTGTGGCAAGGTC TTCTTTAAGATCAAAAGCCGGAATGCGCACATGAAAACCCACCGGCAGCAAGAGGAGCAGCAGAGGCAGA AGGCTCAGAAGGCAGCCTTCGCAGCAGAAATGGCAGCCACCATCGAGAGGACTACGGGGCCGGTGGGGGC GCCCGAGCTGCTGCCCCTGGACCAACTGAGTCTGATGAAGCCAGTCAAGGACGTGGACATCCTGGATGAC GATGTGGTCCAGCAGTTAGGCGTCATGGACGAGGCCGAGGTGGTAGGCACGGATCTTCTCTTGGACGACC AAGATTCGGTTTTGCTTCAGGGTGATACAGAACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211828 representing BC059215 Red=Cloning site Green=Tags(s) MGDQQLYKTNHVGHGGENLFYQQPPLGVHSGLGHSYGNTISGAGMDAPQASPISPHFPQDTRDGLGLPIG SKNLGQMDTSRQGGWGSHVGPGNHVQLRSNLANSNMMWGTPTQVEPADGYQYTYSQASEIRTQKLTSGVL HKLDSFTQVFANQNLRIQVNNMAQVLHTQSAVMDGASDSALRQLLSQKPVEPSASAIASRYQQVPQQPHP GFTGGLPKPALPVGQHAPQGHLYYDYQQPLAQMSMQGGQPLQAPQVLSGHMQQLQQHQYYPQPPPQQQQA GLQRISVQEMQQQQQPQQIRPSPPQQQQQLQLQQRQSSLQIPQYYQPQPMMQHLQEQQQPSMHLQPPSYH RDPHQYTPEQAHAVQLIQLGSMPQYYYQEPQQAYSHPLYPQSHLSQHQQREDGQLKTYSSDRQTPAMLSS HGDMGTSDTGVADPASSEMTRVTSTLPHQPLLSPSGIHLNNMGSQHQQPPSPSAMWPQMHLPDGRAQSGS PESSSGQTKGVFGEQFDAKNKLTCSICLKEFKSLPALNGHMRSHGGMRASPSLKQEEGEKAPPPQPQPQP QPQQPLPPPPPPPPPPQLPPEAERLTPMVMPVSVPVKLIPPKPSSQGFTNSVAATPAARDKPASSMSDDE MPVLVRMTLSPPHSPQGAAPRAPAEIPRKHPPIAAKVEEPLKNLPEKKKFRHRPEPLFIPPPPSSYTPNP TSYSGATLYQSQLRSPRILGDHLLLDPAHELPPYTPPPMLSPVRQGSGLFSNVLISGHGPGVHPQLPLTP LTPTPRVLLCRSSSIDGSNVTVTPGPGEQTVDVEPRINIGLRFQAEIPELQDVSALAQDTHRATLVWKPW PELENQALQQQVENLLNLCCSSALPGGGTNSEFALHSLFEAKGDVMATLEMLLLRKPVRLKCHPLANYHY AGSDKWTSLERKLFNKALATYSKDFIFVQKMVKSKTVAQCVEYYYTWKKIMRLGRKHRTRLAEIIDDCMT SEEEEEAEEEEEDPEEDRKSIKEEESEVAKSPEPPPAPALAPTEGPPMQAVGQQPSGNFICEMPNCGAVF SSRQALNGHARIHGGTNQVAKTRGAIPSGKQKPGGTQSGYCSVKSSPSHSTTSGETDPTTIFPCKECGKV FFKIKSRNAHMKTHRQQEEQQRQKAQKAAFAAEMAATIERTTGPVGAPELLPLDQLSLMKPVKDVDILDD DVVQQLGVMDEAEVVGTDLLLDDQDSVLLQGDTEL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC059215
ORF Size	3677 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC059215, AAH59215
RefSeq Size	4555 bp
RefSeq ORF	3677 bp
Locus ID	224829
Gene Summary	Binds DNA and activates transcription of CYP11A1. Interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1.[UniProtKB/Swiss-Prot Function]

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