Cilp (BC080666) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	9830114D16; C130036G17Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211772 representing BC080666 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCAATCAAGACTTGGGTATTCTCTTTCCTGGTCCTTGAGGTCACCACCGTGCTGGGGAGACAGA CGATGCTTGCCCAGTCAGTGAGAAGAGTCCAGCCTGTGAAGAGGACCCCCAAGACCCTTGCCAAGCCTGC CGACTCCCAGGAGAGTCCCGGAGAGTGGACAACATGGTTTAACATCGACCACCCAGGTGGGCAGGGTGAC TATGAGCGGCTGGATGCCATTCGCTTCTACTATGGGGAGCGTGTGTGTGCCCGTCCCCTGCGGCTAGAGG CCCGGACCACTGACTGGATGCCTGCGGGCAGCACTGGCCAGGTGGTCCACGGCAGTCCCCGTGAGGGCTT CTGGTGCCTCAACAGGGAGCAGAGACCAGGCCAGAACTGTTCCAATTATACCGTGCGCTTCCTCTGCCCA CCAGGGTCCTTGCGAGGAGATGCAGAGCACATCTGGAGTTCCTGGTCTCCCTGGAGCAAGTGCTCAGCTG CCTGCGGTCACACTGGGGTGCAGACCCGTACCCGCACCTGCTTGGCACAGACAGTGTCACTGTGCAGTGA GGCGACCGAGGAGGGCCAGCTCTGCATGAGCCAGGCCTGCACAGCTTGTGACCTGACCTGCCCCATGGGC CAGGTAAATGCTGACTGTGATGCCTGCATGTGCCAGGACTTCATGCTTCACGGGGCCATCTCCCTCCCTG GAGGTGGCCCAGCTCCAGGAGCCGCTGTCTACCTGCTGGCCAAGGCACCAAAGATGCTGACCCGAACAGA CAGCAGCGGGAGGTTCCGAGTTCCTGGCTTGTGTCCTGATGGCAAAACCATCCTGAAAATCACCAAGACC AAGTTTGCCCCAATTATGATCACGATGCCCAAGACTAGCCTGAAGTCAGCTACCATCAATGCGGAGTTTG TGAGGGCAGAGACCCCATACATTGTAATGAACCCTGAGATGAAAGCACGTCGGGCTGGGCAGAGTGTGTC TCTGTGCTGCAAAGCCACGGGGAAACCCAGTCCGGACAAGTACTTCTGGTACCACAACAACACACTGCTG GATCCCTCCCTCTATAAGCACGAGAGCAAGCTGGTGCTGAGGAACCTGCAGCAGGACCAAGCAGGGGAGT ACTTCTGTAAGGCTCAGAGTGATGCTGGGGCTGTGAAGTCCAAGGTCACCCAGCTCACTGTCATTGCACA TGATGAGACTCCTTGCAACCCAACCCCAGAGAGCTACCTTATCCGGCTGCCCCATGACTGCTTTCAGAAC GCCAGCAATTCCTTCTACTACGACGTAGGCCGCTGCCCGATCAAGACCTGTGCAGGGCAGCAGGACAATG GGATCCGGTGCCGAGATGCTGTGGAGAACTGCTGCGGCATTTCCAGAACAGAAGAGAGGGAGATCCAGTG CAGTGGGTACACACTGCCCACCAAGGTGGCTGTGGAGTGCAGCTGCCAACGATGCGCAGAGACACGGAGC ATCGTCCGGGGCCGAGTCACTGCTACTGACAACGGGGAGCCCATGCGCTTTGGCCATGTGTACATGGGGA ATAACCGCGTGAGCATGACTGGCTACAAAGGCACCTTCACACTCCACATCCCCCAGGACACGGAGAGGCT GGTGCTCACATTTGTGGACAGGCTGCAGAAGTTTGTCAACACAACCAAAGTGCTGCCCTTCAACAAGAAG GGAAGCGCAGTGTTCCACGAGATCAAGATGCTTCGGCAGAAAGAGCCCATCACGTTGGAAGCCATGGAGA CCAACATTATCCCCCTGGGAGAGGTGATTGGTGAAGACCCTGTGGCTGAGCTGGAGATTCCATCCAAAAG TTTCTATAGGCAGAACGGGGAGCCCTTCACAGGAAAGGTAAAGGCCAGCGTGACCTTCCTGGATCCTCGG AACATCTCGACGGCCACAGCTGCCCAAAGTGACCTCAACTTCATCAATGATGAAGGAGACACTTTTCCCC TTAGAACATATGGTATGTTTTCAGTGGACTTTAGAGATGAGGCCACTTCCGAGTCACTTAATGCTGGCAA GGTGAAGGTCCACCTTGACTCGACCCAGGTCAAAATGCCAGAGCATGTGCCAGCCATGAAACTCTGGTCG CTCAACCCAGACACAGGACTGTGGGAAGAGGAAGGGGATTTCAAGTTTGAAAGCCAAAGGAGGAACAAGA GGGAAGAGAGAACCTTCCTAGTGGGCAATATGGAGATCCGGGAGAGAAGGCTCTTTAACCTGGACGTCCC TGAAAGCCGAAGGTGCTTCATTAAGGTGAGGACTTACCGGAGTGAGAGGTTCCTGCCCAGTGAGCAAATT CAGGGAGTTGTGGTCTCAGTGATCAATCTGGAGCCCAGAACTGGCTTCTCATCTAACCCCAGGGCCTGGG GCCGATTTGACAGTGTCATCACAGGGCCCAATGGGGCCTGCCTGCCTGCCTTCTGTGATGATCAGTCCCC CGATGCTTACTCTGTCTATGTCTTGGCAAGCCTTTCTGGAGAAGAACTAGAGGCAGTTGAGTCTTCTCCT AAATTCAACCCAAATGCAATCGGTGTCCCTCAGCCTTACCTCAACAAACTTAAATACCGTCGGACAGACC ATGAGGATCCACGGGTTAAAAAGACGGCTTTCCAAATCAGTATGGCCAAACCAAGGCCCAACTCAGCTGA GGAGAGCAATGGGCCTATCTATGCCTTTGAGAACCTTCGGGCGTGTGAGGAGGCACCCCCCAGTGCAGCT CACTTCCGGTTCTATCAGATCGAGGGGGATCGCTATGACTATAACACAGTTCCTTTCAATGAAGATGACC CTATGAGCTGGACTGAAGACTACCTGGCATGGTGGCCCAAGCCAATGGAGTTCAGGGCCTGCTATATCAA GGTTAAGATTGTGGGGCCACTGGAGGTGAATGTACGATCCCGTAACATGGGAGGCACCCACCGGCAGACT GTGGGGAAGCTCTATGGGATCCGGGATGTGAAGAGTACTCGGGACAGAGACCAACCTAATGTCTCATCTG CCTGCCTGGAGTTCAAGTGCAGTGGAATGCTGTATGACCAAGACCGTGTAGACCGCACATTAGTAAAGGT TATCCCCCAGGGCAGCTGCCATCGAGCTAGTGTTAACTCTATGTTGCACGAGTACCTGGTCAACCACCTT CCCCTGGCAGTCAACAATGACACCAGCGAGTATACTATGCTGGCACCCCTGGACCCTCTGGGCCACAATT ACGGTATCTATACTGTTACTGATCAGGACCCTCGTACAGCTAAGGAGATTGCGCTCGGCCGGTGCTTTGA TGGCACTTCCGATGGCTCCTCCAGAATCATGAAGAGCAATGTGGGAGTTGCCCTGACCTTTAACTGTGCA GAAAGGCAGGTAGGCCGCCAGAGTGCCTTCCAGTACCTCCAAAGCACACCGGCCAGGTCCCCAGCTACAG GCACTGTCCAAGGAAGAGTACCAGCCATGAGGCAACAACGGGCAAGCAGGGGTGGCCTACGCCGGCGTGG AAGCATGGCCCCTCTGAGATTTTCTGGTGTTGCTCAACAACCTCTGAGCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211772 representing BC080666 Red=Cloning site Green=Tags(s) MAAIKTWVFSFLVLEVTTVLGRQTMLAQSVRRVQPVKRTPKTLAKPADSQESPGEWTTWFNIDHPGGQGD YERLDAIRFYYGERVCARPLRLEARTTDWMPAGSTGQVVHGSPREGFWCLNREQRPGQNCSNYTVRFLCP PGSLRGDAEHIWSSWSPWSKCSAACGHTGVQTRTRTCLAQTVSLCSEATEEGQLCMSQACTACDLTCPMG QVNADCDACMCQDFMLHGAISLPGGGPAPGAAVYLLAKAPKMLTRTDSSGRFRVPGLCPDGKTILKITKT KFAPIMITMPKTSLKSATINAEFVRAETPYIVMNPEMKARRAGQSVSLCCKATGKPSPDKYFWYHNNTLL DPSLYKHESKLVLRNLQQDQAGEYFCKAQSDAGAVKSKVTQLTVIAHDETPCNPTPESYLIRLPHDCFQN ASNSFYYDVGRCPIKTCAGQQDNGIRCRDAVENCCGISRTEEREIQCSGYTLPTKVAVECSCQRCAETRS IVRGRVTATDNGEPMRFGHVYMGNNRVSMTGYKGTFTLHIPQDTERLVLTFVDRLQKFVNTTKVLPFNKK GSAVFHEIKMLRQKEPITLEAMETNIIPLGEVIGEDPVAELEIPSKSFYRQNGEPFTGKVKASVTFLDPR NISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNAGKVKVHLDSTQVKMPEHVPAMKLWS LNPDTGLWEEEGDFKFESQRRNKREERTFLVGNMEIRERRLFNLDVPESRRCFIKVRTYRSERFLPSEQI QGVVVSVINLEPRTGFSSNPRAWGRFDSVITGPNGACLPAFCDDQSPDAYSVYVLASLSGEELEAVESSP KFNPNAIGVPQPYLNKLKYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAA HFRFYQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRNMGGTHRQT VGKLYGIRDVKSTRDRDQPNVSSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCHRASVNSMLHEYLVNHL PLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKSNVGVALTFNCA ERQVGRQSAFQYLQSTPARSPATGTVQGRVPAMRQQRASRGGLRRRGSMAPLRFSGVAQQPLSN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC080666
ORF Size	3554 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC080666, AAH80666
RefSeq Size	4164 bp
RefSeq ORF	3554 bp
Locus ID	214425
Gene Summary	Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage (By similarity).[UniProtKB/Swiss-Prot Function]

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