Card11 (BC096592) Mouse Tagged ORF Clone
CAT#: MG211717
- TrueORF®
Card11 (tGFP-tagged) - Mouse caspase recruitment domain family, member 11 (cDNA clone MGC:106296 IMAGE:5318165)
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CNY 9,220.00
Cited in 3 publications. |
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BIMP3, CARMA1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG211717 representing BC096592
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGAGGAGGGCCAGCTATGGATGACTACATGGAGACGCTGAAGGATGAAGAGGAGGCCCTATGGG ATAACGTGGAATGCAACCGGCACATGCTGAGCCGTTACATCAACCCCGCCAAGCTCACCCCCTACCTGCG CCAGTGCAAGGTCATCGATGAGCAAGATGAAGACGAGGTGCTCAATGCGCCCATGCTGCCGTCCAAGATC AACCGTGCAGGCCGATTGTTGGACATTCTTCACACCAAGGGACAAAGGGGCTATGTGGTCTTCCTGGAGA GCCTGGAGTTTTACTACCCAGAACTTTACAAACTGGTGACTGGAAAGGAACCCACCCGGAGATTCTCCAC CATTGTGGTGGAGGAAGGCCATGAGGGCCTCACACACTTCCTGATGAACGAGGTCATCAAACTGCAGCAG CAAGTGAAAGCCAAGGACCTTCAGCGCTGTGAGCTGCTGGCCAAGTCCCGGCAACTGGAGGATGAGAAGA AGCAGCTGAGCCTGATACGGGTGGAGCTGCTGACCTTCCAGGAGCGATACTACAAGATGAAGGAGGAGCG GGACAGCTACAATGACGAGCTCGTCAAGGTCAAGGACGACAACTACAACTTAGCCATGCGCTACGCCCAG CTCAGTGAGGAGAAAAACATGGCGGTGATGAGGAGCCGCGACCTCCAACTCGAGATCGACCAGCTCAAAC ACCGACTGAACAAGATGGAGGAGGAATGCAAGCTGGAGAGAAATCAGTCCCTCAAGCTCAAGAATGACAT CGAGAACCGGCCCAGGAAGGAGCAGGTCCTGGAGCTGGAGCGGGAGAATGAGATGCTGAAGACGAAAATT CAGGAGCTGCAGTCCATCATCCAGGCTGGCAAGCGCAGCCTCCCTGACTCAGACAAGGCCATCTTGGACA TCCTGGAACATGACCGGAAGGAGGCGCTAGAGGACCGGCAGGAACTGGTCAACAAAATTTACAACCTACA AGAGGAAGTCCGCCAGGCGGAGGAGCTGCGGGATAAGTACCTGGAGGAGAAGGAAGACCTGGAACTCAAG TGTTCAACCCTGGGGAAGGACTGTGAAATGTACAAGCACCGCATGAACACAGTTATGCTGCAGCTGGAGG AGGTGGAGCGGGAGCGGGACCAGGCCTTCCACTCCCGAGATGAGGCACAGACACAGTACTCACAGTGCTT AATCGAGAAGGACAAGTACCGGAAGCAGATCCGGGAGCTGGAGGAGAAGAACGATGAGATGCGTATTGAG ATGGTGAGGAGGGAGGCCTGTATTGTCAACCTGGAAAGCAAGCTCCGGCGCCTGTCCAAGGACAACGGCA GCCTCGACCAGAGTCTGCCTAGACACCTTCCAGCCACCATCATCTCACAGAACCTTGGAGACACCAGCCC CAGGACCAATGGCCAGGAAGCTGATGATTCTTCAACCTCAGAAGAGTCTCCCGAAGACAGCAAGTACTTT CTGCCTTACCACCCACCCCGGCGCCGGATGAACCTAAAGGGCATCCAGCTGCAGAGAGCCAAATCCCCCA TCAGCATGAAGCAAGCATCTGAGTTTCAAGTCAAGGGGCACGAAGAGGATTTCACAGACGGCAGCCCCAG TTCCTCCCGCTCGCTGCCTGTCACCAGCTCTTTCTCCAAGATGCAACCCCATCGGAGCCGCAGCAGCATC ATGTCAATCACGGCAGAGCCCCCGGGAAATGACTCCATAGTCAGACGCTGTAAGGAAGATGCGCCACACC GGAGCACGGTGGAAGAAGACAACGATAGCTGTGGGTTTGATGCCTTAGACCTTGACGATGAAAATCACGA ACGTTATTCCTTTGGACCTCCCTCCATCCACTCCTCCTCCTCTTCACACCAGTCAGAGGGACTGGATGCC TACGACCTGGAGCAGGTCAACCTCATGTTACGAAAGTTCTCTTTGGAAAGGCCCTTCCGGCCATCGGTCA CATCTGGGGGTCACGTGCGGGGCACCGGGCCCTTGGTCCAGCACACAACTCTGAATGGCGATGGGCTCAT CACGCAGCTCACCCTTCTGGGCGGCAATGCACGCGGGAGCTTCATTCACTCTGTCAAGCCAGGCTCACTG GCTGAGAGGGCCGGACTGCGTGAGGGCCACCAACTCCTGCTGCTGGAAGGTTGCATCCGAGGCGAAAGGC AGAGCGTTCCACTGGATGCGTGCACAAAAGAAGAGGCCCGTTGGACCATCCAGAGGTGCAGTGGCCTCAT CACTCTGCATTACAAGGTCAACCATGAAGGATACCGGAAGCTGCTGAAGGAGATGGAGGATGGTCTGATC ACATCAGGGGACTCGTTCTATATCCGCCTGAACCTGAACATCTCCAGCCAGCTGGATGCCTGCTCCATGT CCCTCAAGTGTGACGACGTGGTGCATGTCCTAGACACCATGTACCAGGACAGGCACGAGTGGCTGTGTGC ACGAGTCGACCCCTTCACTGACCAAGACCTGGACACGGGCACCATCCCCAGCTACAGCCGGGCTCAACAG CTTCTCCTGGTGAAGCTCCAGCGGTTGGTTCACAGAGGCAACCGGGAAGAGGCAGACAGCGCTCACCACA CCCTGCGCAGCCTCCGGAACACCCTGCAGCCCGAAGAGATGCTTTCGACGAGCGACCCCCGAGTCAGCCC CCGCCTCTCCAGAGCGAGTTTCTTCTTTGGCCAGCTCCTGCAGTTTGTCAGCCGGTCAGAAAACAAGTAC AAAAGAATGAACAGCAATGAGCGCGTGAGAATCATCTCTGGGAGTCCCCTGGGGAGCCTCTCCCGGTCCT CGCTGGATGCCACCAAACTCCTGACCGAGAAGCATGAAGAACTGGATCCTGAGAATGAGCTCAGCCGGAA CCTCACCCTGATCCCTTACAGCCTGGTGCGCGCTTTCCACTGTGAGCGCCGCAGGCCTGTGCTCTTCACG CCCACCATGCTGGCCAAGACATTGGTGCAGAAGCTGCTCAACTCAGGGGGTGCCATGGAGTTCACCATCT GCAAGTCAGATATTGTCACAAGAGATGAGTTCCTCCGAAAGCAGAAGACAGAGACCATCATCTACTCCCG GGAAAAGAACCCCAACACCTTTGAATGCATCGTCCCTGCCAACATTGAGGCTGTGGCAGCCAAGAACAAA CACTGCCTGCTGGAGGCTGGGATCGGCTGTGTGCGCGACCTGATCAAGTGCAAGGTGTACCCCATAGTGC TGCTCATCCGGGTGAGCGAGAAGAACATCAAACGGTTCAGGAAGCTGCTGCCGCGGCCAGAGACGGAAGA GGAATTCCTGCGAGTGTGCAGGCTCAAAGAGAAGGAGCTGGAGGCGCTGCCCTGCCTCTACGCCACCGTG GAAGCTGAGATGTGGAGCAGCGTGGAGGAGCTGCTGCGAGTCCTCAAAGACAAGATTGTAGAGGAGCAGC GCAAGACCATCTGGGTGGACGAGGACCAGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211717 representing BC096592
Red=Cloning site Green=Tags(s) MPGGGPAMDDYMETLKDEEEALWDNVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNAPMLPSKI NRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEVIKLQQ QVKAKDLQRCELLAKSRQLEDEKKQLSLIRVELLTFQERYYKMKEERDSYNDELVKVKDDNYNLAMRYAQ LSEEKNMAVMRSRDLQLEIDQLKHRLNKMEEECKLERNQSLKLKNDIENRPRKEQVLELERENEMLKTKI QELQSIIQAGKRSLPDSDKAILDILEHDRKEALEDRQELVNKIYNLQEEVRQAEELRDKYLEEKEDLELK CSTLGKDCEMYKHRMNTVMLQLEEVERERDQAFHSRDEAQTQYSQCLIEKDKYRKQIRELEEKNDEMRIE MVRREACIVNLESKLRRLSKDNGSLDQSLPRHLPATIISQNLGDTSPRTNGQEADDSSTSEESPEDSKYF LPYHPPRRRMNLKGIQLQRAKSPISMKQASEFQVKGHEEDFTDGSPSSSRSLPVTSSFSKMQPHRSRSSI MSITAEPPGNDSIVRRCKEDAPHRSTVEEDNDSCGFDALDLDDENHERYSFGPPSIHSSSSSHQSEGLDA YDLEQVNLMLRKFSLERPFRPSVTSGGHVRGTGPLVQHTTLNGDGLITQLTLLGGNARGSFIHSVKPGSL AERAGLREGHQLLLLEGCIRGERQSVPLDACTKEEARWTIQRCSGLITLHYKVNHEGYRKLLKEMEDGLI TSGDSFYIRLNLNISSQLDACSMSLKCDDVVHVLDTMYQDRHEWLCARVDPFTDQDLDTGTIPSYSRAQQ LLLVKLQRLVHRGNREEADSAHHTLRSLRNTLQPEEMLSTSDPRVSPRLSRASFFFGQLLQFVSRSENKY KRMNSNERVRIISGSPLGSLSRSSLDATKLLTEKHEELDPENELSRNLTLIPYSLVRAFHCERRRPVLFT PTMLAKTLVQKLLNSGGAMEFTICKSDIVTRDEFLRKQKTETIIYSREKNPNTFECIVPANIEAVAAKNK HCLLEAGIGCVRDLIKCKVYPIVLLIRVSEKNIKRFRKLLPRPETEEEFLRVCRLKEKELEALPCLYATV EAEMWSSVEELLRVLKDKIVEEQRKTIWVDEDQL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC096592 |
ORF Size | 3464 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC096592, AAH96592 |
RefSeq Size | 4139 bp |
RefSeq ORF | 3464 bp |
Locus ID | 108723 |
Gene Summary | Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation (PubMed:12356734, PubMed:16356855). Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. Also activates the TORC1 signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
Integrated molecular analysis of adult T cell leukemia/lymphoma.
,null,
Nature genetics
,PubMed ID 26437031
[Card11]
|
Novel CARD11 Mutations in Human Cutaneous Squamous Cell Carcinoma Lead to Aberrant NF-κB Regulation
,null,
The American Journal of Pathology
,PubMed ID 26212909
[Card11]
|
CARD11 mediates factor-specific activation of NF-?B by the T cell receptor complex
,null,
,PubMed ID 12356734
[Card11]
|
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