Card11 (BC096592) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	BIMP3, CARMA1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211717 representing BC096592 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGAGGAGGGCCAGCTATGGATGACTACATGGAGACGCTGAAGGATGAAGAGGAGGCCCTATGGG ATAACGTGGAATGCAACCGGCACATGCTGAGCCGTTACATCAACCCCGCCAAGCTCACCCCCTACCTGCG CCAGTGCAAGGTCATCGATGAGCAAGATGAAGACGAGGTGCTCAATGCGCCCATGCTGCCGTCCAAGATC AACCGTGCAGGCCGATTGTTGGACATTCTTCACACCAAGGGACAAAGGGGCTATGTGGTCTTCCTGGAGA GCCTGGAGTTTTACTACCCAGAACTTTACAAACTGGTGACTGGAAAGGAACCCACCCGGAGATTCTCCAC CATTGTGGTGGAGGAAGGCCATGAGGGCCTCACACACTTCCTGATGAACGAGGTCATCAAACTGCAGCAG CAAGTGAAAGCCAAGGACCTTCAGCGCTGTGAGCTGCTGGCCAAGTCCCGGCAACTGGAGGATGAGAAGA AGCAGCTGAGCCTGATACGGGTGGAGCTGCTGACCTTCCAGGAGCGATACTACAAGATGAAGGAGGAGCG GGACAGCTACAATGACGAGCTCGTCAAGGTCAAGGACGACAACTACAACTTAGCCATGCGCTACGCCCAG CTCAGTGAGGAGAAAAACATGGCGGTGATGAGGAGCCGCGACCTCCAACTCGAGATCGACCAGCTCAAAC ACCGACTGAACAAGATGGAGGAGGAATGCAAGCTGGAGAGAAATCAGTCCCTCAAGCTCAAGAATGACAT CGAGAACCGGCCCAGGAAGGAGCAGGTCCTGGAGCTGGAGCGGGAGAATGAGATGCTGAAGACGAAAATT CAGGAGCTGCAGTCCATCATCCAGGCTGGCAAGCGCAGCCTCCCTGACTCAGACAAGGCCATCTTGGACA TCCTGGAACATGACCGGAAGGAGGCGCTAGAGGACCGGCAGGAACTGGTCAACAAAATTTACAACCTACA AGAGGAAGTCCGCCAGGCGGAGGAGCTGCGGGATAAGTACCTGGAGGAGAAGGAAGACCTGGAACTCAAG TGTTCAACCCTGGGGAAGGACTGTGAAATGTACAAGCACCGCATGAACACAGTTATGCTGCAGCTGGAGG AGGTGGAGCGGGAGCGGGACCAGGCCTTCCACTCCCGAGATGAGGCACAGACACAGTACTCACAGTGCTT AATCGAGAAGGACAAGTACCGGAAGCAGATCCGGGAGCTGGAGGAGAAGAACGATGAGATGCGTATTGAG ATGGTGAGGAGGGAGGCCTGTATTGTCAACCTGGAAAGCAAGCTCCGGCGCCTGTCCAAGGACAACGGCA GCCTCGACCAGAGTCTGCCTAGACACCTTCCAGCCACCATCATCTCACAGAACCTTGGAGACACCAGCCC CAGGACCAATGGCCAGGAAGCTGATGATTCTTCAACCTCAGAAGAGTCTCCCGAAGACAGCAAGTACTTT CTGCCTTACCACCCACCCCGGCGCCGGATGAACCTAAAGGGCATCCAGCTGCAGAGAGCCAAATCCCCCA TCAGCATGAAGCAAGCATCTGAGTTTCAAGTCAAGGGGCACGAAGAGGATTTCACAGACGGCAGCCCCAG TTCCTCCCGCTCGCTGCCTGTCACCAGCTCTTTCTCCAAGATGCAACCCCATCGGAGCCGCAGCAGCATC ATGTCAATCACGGCAGAGCCCCCGGGAAATGACTCCATAGTCAGACGCTGTAAGGAAGATGCGCCACACC GGAGCACGGTGGAAGAAGACAACGATAGCTGTGGGTTTGATGCCTTAGACCTTGACGATGAAAATCACGA ACGTTATTCCTTTGGACCTCCCTCCATCCACTCCTCCTCCTCTTCACACCAGTCAGAGGGACTGGATGCC TACGACCTGGAGCAGGTCAACCTCATGTTACGAAAGTTCTCTTTGGAAAGGCCCTTCCGGCCATCGGTCA CATCTGGGGGTCACGTGCGGGGCACCGGGCCCTTGGTCCAGCACACAACTCTGAATGGCGATGGGCTCAT CACGCAGCTCACCCTTCTGGGCGGCAATGCACGCGGGAGCTTCATTCACTCTGTCAAGCCAGGCTCACTG GCTGAGAGGGCCGGACTGCGTGAGGGCCACCAACTCCTGCTGCTGGAAGGTTGCATCCGAGGCGAAAGGC AGAGCGTTCCACTGGATGCGTGCACAAAAGAAGAGGCCCGTTGGACCATCCAGAGGTGCAGTGGCCTCAT CACTCTGCATTACAAGGTCAACCATGAAGGATACCGGAAGCTGCTGAAGGAGATGGAGGATGGTCTGATC ACATCAGGGGACTCGTTCTATATCCGCCTGAACCTGAACATCTCCAGCCAGCTGGATGCCTGCTCCATGT CCCTCAAGTGTGACGACGTGGTGCATGTCCTAGACACCATGTACCAGGACAGGCACGAGTGGCTGTGTGC ACGAGTCGACCCCTTCACTGACCAAGACCTGGACACGGGCACCATCCCCAGCTACAGCCGGGCTCAACAG CTTCTCCTGGTGAAGCTCCAGCGGTTGGTTCACAGAGGCAACCGGGAAGAGGCAGACAGCGCTCACCACA CCCTGCGCAGCCTCCGGAACACCCTGCAGCCCGAAGAGATGCTTTCGACGAGCGACCCCCGAGTCAGCCC CCGCCTCTCCAGAGCGAGTTTCTTCTTTGGCCAGCTCCTGCAGTTTGTCAGCCGGTCAGAAAACAAGTAC AAAAGAATGAACAGCAATGAGCGCGTGAGAATCATCTCTGGGAGTCCCCTGGGGAGCCTCTCCCGGTCCT CGCTGGATGCCACCAAACTCCTGACCGAGAAGCATGAAGAACTGGATCCTGAGAATGAGCTCAGCCGGAA CCTCACCCTGATCCCTTACAGCCTGGTGCGCGCTTTCCACTGTGAGCGCCGCAGGCCTGTGCTCTTCACG CCCACCATGCTGGCCAAGACATTGGTGCAGAAGCTGCTCAACTCAGGGGGTGCCATGGAGTTCACCATCT GCAAGTCAGATATTGTCACAAGAGATGAGTTCCTCCGAAAGCAGAAGACAGAGACCATCATCTACTCCCG GGAAAAGAACCCCAACACCTTTGAATGCATCGTCCCTGCCAACATTGAGGCTGTGGCAGCCAAGAACAAA CACTGCCTGCTGGAGGCTGGGATCGGCTGTGTGCGCGACCTGATCAAGTGCAAGGTGTACCCCATAGTGC TGCTCATCCGGGTGAGCGAGAAGAACATCAAACGGTTCAGGAAGCTGCTGCCGCGGCCAGAGACGGAAGA GGAATTCCTGCGAGTGTGCAGGCTCAAAGAGAAGGAGCTGGAGGCGCTGCCCTGCCTCTACGCCACCGTG GAAGCTGAGATGTGGAGCAGCGTGGAGGAGCTGCTGCGAGTCCTCAAAGACAAGATTGTAGAGGAGCAGC GCAAGACCATCTGGGTGGACGAGGACCAGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211717 representing BC096592 Red=Cloning site Green=Tags(s) MPGGGPAMDDYMETLKDEEEALWDNVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNAPMLPSKI NRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEVIKLQQ QVKAKDLQRCELLAKSRQLEDEKKQLSLIRVELLTFQERYYKMKEERDSYNDELVKVKDDNYNLAMRYAQ LSEEKNMAVMRSRDLQLEIDQLKHRLNKMEEECKLERNQSLKLKNDIENRPRKEQVLELERENEMLKTKI QELQSIIQAGKRSLPDSDKAILDILEHDRKEALEDRQELVNKIYNLQEEVRQAEELRDKYLEEKEDLELK CSTLGKDCEMYKHRMNTVMLQLEEVERERDQAFHSRDEAQTQYSQCLIEKDKYRKQIRELEEKNDEMRIE MVRREACIVNLESKLRRLSKDNGSLDQSLPRHLPATIISQNLGDTSPRTNGQEADDSSTSEESPEDSKYF LPYHPPRRRMNLKGIQLQRAKSPISMKQASEFQVKGHEEDFTDGSPSSSRSLPVTSSFSKMQPHRSRSSI MSITAEPPGNDSIVRRCKEDAPHRSTVEEDNDSCGFDALDLDDENHERYSFGPPSIHSSSSSHQSEGLDA YDLEQVNLMLRKFSLERPFRPSVTSGGHVRGTGPLVQHTTLNGDGLITQLTLLGGNARGSFIHSVKPGSL AERAGLREGHQLLLLEGCIRGERQSVPLDACTKEEARWTIQRCSGLITLHYKVNHEGYRKLLKEMEDGLI TSGDSFYIRLNLNISSQLDACSMSLKCDDVVHVLDTMYQDRHEWLCARVDPFTDQDLDTGTIPSYSRAQQ LLLVKLQRLVHRGNREEADSAHHTLRSLRNTLQPEEMLSTSDPRVSPRLSRASFFFGQLLQFVSRSENKY KRMNSNERVRIISGSPLGSLSRSSLDATKLLTEKHEELDPENELSRNLTLIPYSLVRAFHCERRRPVLFT PTMLAKTLVQKLLNSGGAMEFTICKSDIVTRDEFLRKQKTETIIYSREKNPNTFECIVPANIEAVAAKNK HCLLEAGIGCVRDLIKCKVYPIVLLIRVSEKNIKRFRKLLPRPETEEEFLRVCRLKEKELEALPCLYATV EAEMWSSVEELLRVLKDKIVEEQRKTIWVDEDQL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC096592
ORF Size	3462 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC096592, AAH96592
RefSeq Size	4139 bp
RefSeq ORF	3464 bp
Locus ID	108723
Gene Summary	Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation (PubMed:12356734, PubMed:16356855). Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. Also activates the TORC1 signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function]

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