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Myo1f (NM_053214) Mouse Tagged ORF Clone
CAT＃: MG211630
- TrueORF®
Myo1f (tGFP-tagged) - Mouse myosin IF (Myo1f)
ORF Plasmid: DDK
"NM_053214" in other vectors (3)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C330006B10Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211630 representing NM_053214
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCAAGGAGCGCTTTCACTGGCAGAGTCACAACGTGAAGCAGAGTGGAGTGGATGACATGGTGC TGCTGCCCCAGATCACCGAGGACGCCATTGTGAGCAACCTCCGCAAACGCTTCATGGATGACTACATTTT TACATACATTGGCTCAGTGCTAATCTCTGTAAACCCCTTCAAGCAGATGCCCTACTTCACTGACCGAGAA ATTGACCTCTATCAGGGCGCGGCTCAATATGAGAATCCCCCGCACATCTATGCCCTCACCGACAACATGT ACCGGAATATGCTGATCGACTGTGAGAACCAGTGTGTCATTATCAGTGGGGAGAGCGGAGCTGGGAAGAC AGTGGCTGCTAAATACATCATGGGCTACATCTCCAAGGTGTCTGGTGGGGGAGACAAGGTCCAGCACGTC AAAGACATAATCCTACAGTCAAACCCACTGCTTGAGGCTTTCGGCAATGCCAAGACCGTGCGCAACAACA ATTCCAGCCGCTTTGGCAAGTACTTTGAGATCCAGTTCAGTCGAGGTGGAGAGCCAGATGGAGGCAAGAT CTCCAACTTCCTGCTGGAAAAGTCTCGTGTGGTCATGCAGAATGAGAATGAGAGGAACTTCCACATCTAC TACCAGCTTCTGGAAGGGGCCTCCCAGGAGCAGCAGCAGAACCTAGGACTCATGACGCCAGACTACTACT ACTACCTCAACCAATCAGACACCTACAAGGTGGAAGGCACTGATGACCGAAGTGACTTCAGCGAGACCCT GAGCGCCATGCAAGTCATCGGGATTCCGACAAGCGTGCAGCAGCTTGTCTTGCAGCTCGTGGCAGGGATC TTGCACCTGGGGAACATCAGCTTCTGTGAAGAAGGGAATTATGCCCGCGTGGAGAGCGTAGACCTCTTAG CTTTCCCAGCCTACCTGCTGGGCATAGACAGTGGGCGGCTGCAAGAGAAGCTGACCAGCCGTAAGATGGA CAGCAAATGGGGTGGACGCAGTGAGTCCATTGATGTGACCCTCAATGTGGAACAGGCAGCCTACACTCGT GACGCACTGGCCAAGGGACTCTATGCCCGCCTCTTCGACTTCCTGGTGGAGGCCATCAACCGTGCCATGC AGAAGCCTCAGGAAGAATACAGCATCGGCGTGCTTGACATCTACGGCTTTGAGATCTTCCAGAAAAATGG CTTTGAGCAGTTCTGCATCAACTTCGTCAATGAAAAGCTGCAGCAGATTTTTATTGAGCTCACGCTGAAG GCAGAGCAGGAGGAGTATGTGCAAGAGGGCATCCGCTGGACCCCCATCGAATACTTCAACAACAAAATTG TGTGTGACCTCATCGAAAACAAGCTGAGCCCCCCAGGCATCATGAGCGTGCTAGATGATGTGTGTGCCAC GATGCATGCTACCGGTGGCGGCGCGGACCAGACACTGCTACAAAAGCTGCAAGCGGCAGTGGGCACCCAC GAGCACTTCAACAGCTGGAGCGCGGGTTTCGTCATCCATCACTATGCAGGCAAGGTCTCCTATGATGTCA GTGGCTTCTGTGAGAGGAATCGAGATGTGCTGTTCTCTGATCTTATAGAACTGATGCAGTCCAGTGACCA GGACTTCCTACGGATGCTCTTTCCTGAGAAGCTGAATATAGACAAGAAGGGCCGCCCCAGTACTGCTGGC TCCAAGATCAAGAAACAAGCCAACGACCTGGTGTCCACGCTGAAGAAGTGCACACCCCACTACATCCGCT GCATCAAACCTAACGAGACCAAGCGTCCCCGGGACTGGGAGGAAAGCAGAGTGAAGCACCAGGTGGAGTA CCTGGGCCTGCGGGAGAACATCCGAGTGCGAAGGGCAGGCTTCGCCTACCGTCGGCAGTTCTCCAAATTC CTGCAAAGGTATGCCATCCTGACCCCTGAGACATGGCCACGGTGGCGTGGAGATGAACGCCAGGGTGTCC AGCACCTGCTTCGTGCTGTCAACATGGAGCCAGACCAGTACCAGATGGGGAGTACCAAGGTCTTTGTTAA GAATCCTGAGTCGCTTTTCCTCCTGGAAGAGATGCGAGAGAGGAAGTTTGACGGCTTCGCTCGCACTATC CAGAAAGCCTGGCGGCGCCATGTAGCAGTCCGAAAGTATGAAGAGATGCGGGAAGAAGCTTCTAACATCC TACTGAACAAGAAGGAACGAAGGCGAAACAGCATCAACCGTAACTTCGTTGGGGACTATCTGGGACTGGA GGAGCGGCCTGAGCTGCGCCAGTTCCTGGCTAAAAGGGAACGAGTGGACTTTGCCGACTCAGTCACCAAG TATGACCGCCGCTTCAAGCCTATCAAACGGGACTTGATCCTGACCCCAAAGTGTGTGTATGTGATTGGGA GAGAGAAGGTGAAAAGGGGACCAGAGAAGGGTCTAGTGCGTGAAGTCCTGAAGAAGAAACTGGATATCCA GGCCCTCCGAGGGGTGTCTCTCAGCACCCGACAGGATGACTTCTTCATCCTTCAAGAAGAGGCTGCGGAC AGCTTCTTAGAAAGTATCTTCAAGACCGAGTTTGTCAGCCTTCTGTGCAAGCGTTTTGAAGAGGCAGCGC GCAGGCCCCTTCCCCTCACCTTCAGTGACCTACTACAGTTCCGGGTGAAGAAAGAAGGCTGGGGCGGAGG TAGTACCCGAAATGTCACCTTCTCTCGCGGGACAAGTGACTTGGCTGTGCTCAAGGCTGGGGGTCGAGCT CTCACTATCAGCATTGGGGACGGATTGCCTAAGAGCACCAAGCCTACACGGAAGGGGTTAGCCCAGGGTA GACCTCGAAGGTCAGCGCAGGCTCCCACTCGAGCAGCCCCTGGGCCTCCCAGAGGTTTGAATCGAAATGG GGTACCACCCTCTTCCCAGGTAAGGTCCCTACCCCTGGAGATCACATCTGGTAGGAGTTCCCAGAGGCCT CCACGGGGCCCTCCATCCTCAACCCTAGGAGCCAGCAGACGTCCTCGAGCACGGCCACCTTCAGAGCACA GTACAGAATTCCTTAATGTGCCTGACCAGGGTGTGGCAGGCATGCAAAGGAAGCGAAGTATAGGGCAGAG ACCTGTGCCAGGAGTGGGCCGGCCTAAGCCCCAACCACGGACACATGGCCCGAGGTGCCGGGCACTGTAC CAATACATAGGCCAGGATGTGGATGAGCTGAGCTTTAACGTGAACGAAGTCATTGAGATCCTCATAGAAG ACTCTTCTGGCTGGTGGAAGGGTCGGCTTCATGGCCAGGAAGGTCTCTTTCCAGGAAACTATGTGGAGAA GATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211630 representing NM_053214
Red=Cloning site Green=Tags(s) MGSKERFHWQSHNVKQSGVDDMVLLPQITEDAIVSNLRKRFMDDYIFTYIGSVLISVNPFKQMPYFTDRE IDLYQGAAQYENPPHIYALTDNMYRNMLIDCENQCVIISGESGAGKTVAAKYIMGYISKVSGGGDKVQHV KDIILQSNPLLEAFGNAKTVRNNNSSRFGKYFEIQFSRGGEPDGGKISNFLLEKSRVVMQNENERNFHIY YQLLEGASQEQQQNLGLMTPDYYYYLNQSDTYKVEGTDDRSDFSETLSAMQVIGIPTSVQQLVLQLVAGI LHLGNISFCEEGNYARVESVDLLAFPAYLLGIDSGRLQEKLTSRKMDSKWGGRSESIDVTLNVEQAAYTR DALAKGLYARLFDFLVEAINRAMQKPQEEYSIGVLDIYGFEIFQKNGFEQFCINFVNEKLQQIFIELTLK AEQEEYVQEGIRWTPIEYFNNKIVCDLIENKLSPPGIMSVLDDVCATMHATGGGADQTLLQKLQAAVGTH EHFNSWSAGFVIHHYAGKVSYDVSGFCERNRDVLFSDLIELMQSSDQDFLRMLFPEKLNIDKKGRPSTAG SKIKKQANDLVSTLKKCTPHYIRCIKPNETKRPRDWEESRVKHQVEYLGLRENIRVRRAGFAYRRQFSKF LQRYAILTPETWPRWRGDERQGVQHLLRAVNMEPDQYQMGSTKVFVKNPESLFLLEEMRERKFDGFARTI QKAWRRHVAVRKYEEMREEASNILLNKKERRRNSINRNFVGDYLGLEERPELRQFLAKRERVDFADSVTK YDRRFKPIKRDLILTPKCVYVIGREKVKRGPEKGLVREVLKKKLDIQALRGVSLSTRQDDFFILQEEAAD SFLESIFKTEFVSLLCKRFEEAARRPLPLTFSDLLQFRVKKEGWGGGSTRNVTFSRGTSDLAVLKAGGRA LTISIGDGLPKSTKPTRKGLAQGRPRRSAQAPTRAAPGPPRGLNRNGVPPSSQVRSLPLEITSGRSSQRP PRGPPSSTLGASRRPRARPPSEHSTEFLNVPDQGVAGMQRKRSIGQRPVPGVGRPKPQPRTHGPRCRALY QYIGQDVDELSFNVNEVIEILIEDSSGWWKGRLHGQEGLFPGNYVEKI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053214 |
| ORF Size | 3294 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053214.2, NP_444444.2 |
| RefSeq Size | 3833 bp |
| RefSeq ORF | 3297 bp |
| Locus ID | 17916 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR211630 | Myo1f (Myc-DDK-tagged) - Mouse myosin IF (Myo1f) |
CNY 7,648.00 |
|
| MR211630L3 | Lenti ORF clone of Myo1f (Myc-DDK-tagged) - Mouse myosin IF (Myo1f) |
CNY 10,260.00 |
|
| MR211630L4 | Lenti ORF clone of Myo1f (mGFP-tagged) - Mouse myosin IF (Myo1f) |
CNY 10,260.00 |
