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Tek (BC050824) Mouse Tagged ORF Clone
CAT＃: MG211587
- TrueORF®
Tek (tGFP-tagged) - Mouse endothelial-specific receptor tyrosine kinase (cDNA clone MGC:59299 IMAGE:6333576)
ORF Plasmid: DDK
"BC050824" in other vectors (4)
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CNY 11,780.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA517024; Cd202b; Hyk; STK1; Tie-2; Tie2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211587 representing BC050824
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCTTTAGCCGGCTTAGTTCTCTGTGGAGTCAGCTTGCTCCTTTATGGAGTAGTAGAAGGCGCCA TGGACCTGATCTTGATCAATTCCCTACCTCTTGTGTCTGATGCCGAAACATCCCTCACCTGCATTGCCTC TGGGTGGCACCCCCATGAGCCCATCACCATAGGAAGGGACTTTGAAGCCTTAATGAACCAGCACCAAGAT CCACTGGAGGTTACTCAAGATGTGACCAGAGAATGGGCGAAAAAAGTTGTTTGGAAGAGAGAAAAGGCCA GTAAGATTAATGGTGCTTATTTCTGTGAAGGTCGAGTTCGAGGACAGGCTATAAGGATACGGACCATGAA GATGCGTCAACAAGCATCCTTCCTACCTGCTACTTTAACTATGACCGTGGACAGGGGAGATAATGTGAAC ATATCTTTCAAAAAGGTGTTAATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGATGTGAAGCTCAGA AGTGGGGGCCCGACTGTAGCCGTCCTTGTACTACTTGCAAGAACAATGGAGTCTGCCATGAAGATACCGG GGAATGCATTTGCCCTCCTGGGTTTATGGGGAGAACATGTGAGAAAGCTTGTGAGCCGCACACATTTGGC AGGACCTGTAAAGAAAGGTGTAGTGGACCAGAAGGATGCAAGTCTTATGTGTTCTGTCTCCCAGACCCTT ACGGGTGTTCCTGTGCCACAGGCTGGAGGGGGTTGCAGTGCAATGAAGCATGCCCATCTGGTTACTACGG ACCAGACTGTAAGCTCAGGTGCCACTGTACCAATGAAGAGATATGTGATCGGTTCCAAGGATGCCTCTGC TCTCAAGGATGGCAAGGGCTGCAGTGTGAGAAAGAAGGCAGGCCAAGGATGACTCCACAGATAGAGGATT TGCCAGATCACATTGAAGTAAACAGTGGAAAATTTAACCCCATCTGCAAAGCCTCTGGGTGGCCACTACC TACTAGTGAAGAAATGACCCTAGTGAAGCCAGATGGGACAGTGCTCCAACCAAATGACTTCAACTATACA GATCGTTTCTCAGTGGCCATATTCACTGTCAACCGAGTCTTACCTCCTGACTCAGGAGTCTGGGTCTGCA GTGTGAACACAGTGGCTGGGATGGTGGAAAAGCCTTTCAACATTTCCGTCAAAGTTCTTCCAGAGCCCCT GCACGCCCCAAATGTGATTGACACTGGACATAACTTTGCTATCATCAATATCAGCTCTGAGCCTTACTTT GGGGATGGACCCATCAAATCCAAGAAGCTTTTCTATAAACCTGTCAATCAGGCCTGGAAATACATTGAAG TGACGAATGAGATTTTCACTCTCAACTACTTGGAGCCGCGGACTGACTACGAGCTGTGTGTGCAGCTGGC CCGTCCTGGAGAGGGTGGAGAAGGGCATCCTGGGCCTGTGAGACGATTTACAACAGCGTCTATCGGACTC CCTCCTCCAAGAGGTCTCAGTCTCCTGCCAAAAAGCCAGACAGCTCTAAATTTGACTTGGCAACCGATAT TTACAAACTCAGAAGATGAATTTTATGTGGAAGTCGAGAGGCGATCCCTGCAAACAACAAGTGATCAGCA GAACATCAAAGTGCCTGGGAACCTGACCTCGGTGCTACTGAGCAACTTAGTCCCCAGGGAGCAGTACACA GTCCGAGCTAGAGTCAACACCAAGGCGCAGGGGGAGTGGAGTGAAGAACTCAGGGCCTGGACCCTTAGTG ACATTCTCCCTCCTCAACCAGAAAACATCAAGATCTCCAACATCACTGACTCCACAGCTATGGTTTCTTG GACAATAGTGGATGGCTATTCGATTTCTTCCATCATCATCCGGTATAAGGTTCAGGGCAAAAATGAAGAC CAGCACATTGATGTGAAGATCAAGAATGCTACCGTTACTCAGTACCAGCTCAAGGGCCTAGAGCCAGAGA CTACATACCATGTGGATATTTTTGCTGAGAACAACATAGGATCAAGCAACCCAGCCTTTTCTCATGAACT GAGGACGCTTCCACATTCCCCAGCCTCTGCAGACCTCGGAGGGGGAAAGATGCTACTCATAGCCATCCTT GGGTCGGCTGGAATGACTTGCATCACCGTGCTGTTGGCGTTTCTGATTATGTTGCAACTGAAGAGAGCAA ATGTCCAAAGGAGAATGGCTCAGGCATTCCAGAACGTGAGAGAAGAACCAGCTGTGCAGTTTAACTCAGG AACTCTGGCCCTTAACAGGAAGGCCAAAAACAATCCGGATCCCACAATTTATCCTGTGCTTGACTGGAAT GACATCAAGTTTCAAGACGTGATCGGAGAGGGCAACTTTGGCCAGGTTCTGAAGGCACGCATCAAGAAGG ATGGGTTACGGATGGATGCCGCCATCAAGAGGATGAAAGAGTATGCCTCCAAAGATGATCACAGGGACTT CGCAGGAGAACTGGAGGTTCTTTGTAAACTTGGACACCATCCAAACATCATTAATCTCTTGGGAGCATGT GAACACCGAGGCTATTTGTACCTAGCTATTGAGTATGCCCCGCATGGAAACCTCCTGGACTTCCTGCGTA AGAGCAGAGTGCTAGAGACAGACCCTGCTTTTGCCATCGCCAACAGTACAGCTTCCACACTGTCCTCCCA ACAGCTTCTTCATTTTGCTGCAGATGTGGCCCGGGGGATGGACTACTTGAGCCAGAAACAGTTTATCCAC AGGGACCTGGCTGCCAGAAACATTTTAGTTGGTGAAAACTACATAGCCAAAATAGCAGATTTTGGATTGT CACGAGGTCAAGAAGTGTATGTGAAAAAGACAATGGGAAGGCTCCCAGTGCGTTGGATGGCAATCGAATC ACTGAACTATAGTGTCTATACAACCAACAGTGATGTCTGGTCCTATGGTGTATTGCTCTGGGAGATTGTT AGCTTAGGAGGCACCCCCTACTGCGGCATGACGTGCGCGGAGCTCTATGAGAAGCTACCCCAGGGCTACA GGCTGGAGAAGCCCCTGAACTGTGATGATGAGGTGTATGATCTAATGAGACAGTGCTGGAGGGAGAAGCC TTATGAGAGACCATCATTTGCCCAGATATTGGTGTCCTTAAACAGGATGCTGGAAGAACGGAAGACATAC GTGAACACCACACTGTATGAGAAGTTTACCTATGCAGGAATTGACTGCTCTGCGGAAGAAGCAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211587 representing BC050824
Red=Cloning site Green=Tags(s) MDSLAGLVLCGVSLLLYGVVEGAMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQD PLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVN ISFKKVLIKEEDAVIYKNGCEAQKWGPDCSRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFG RTCKERCSGPEGCKSYVFCLPDPYGCSCATGWRGLQCNEACPSGYYGPDCKLRCHCTNEEICDRFQGCLC SQGWQGLQCEKEGRPRMTPQIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNYT DRFSVAIFTVNRVLPPDSGVWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYF GDGPIKSKKLFYKPVNQAWKYIEVTNEIFTLNYLEPRTDYELCVQLARPGEGGEGHPGPVRRFTTASIGL PPPRGLSLLPKSQTALNLTWQPIFTNSEDEFYVEVERRSLQTTSDQQNIKVPGNLTSVLLSNLVPREQYT VRARVNTKAQGEWSEELRAWTLSDILPPQPENIKISNITDSTAMVSWTIVDGYSISSIIIRYKVQGKNED QHIDVKIKNATVTQYQLKGLEPETTYHVDIFAENNIGSSNPAFSHELRTLPHSPASADLGGGKMLLIAIL GSAGMTCITVLLAFLIMLQLKRANVQRRMAQAFQNVREEPAVQFNSGTLALNRKAKNNPDPTIYPVLDWN DIKFQDVIGEGNFGQVLKARIKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGAC EHRGYLYLAIEYAPHGNLLDFLRKSRVLETDPAFAIANSTASTLSSQQLLHFAADVARGMDYLSQKQFIH RDLAARNILVGENYIAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEIV SLGGTPYCGMTCAELYEKLPQGYRLEKPLNCDDEVYDLMRQCWREKPYERPSFAQILVSLNRMLEERKTY VNTTLYEKFTYAGIDCSAEEAA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC050824 |
| ORF Size | 3216 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC050824.1 |
| RefSeq Size | 4549 bp |
| RefSeq ORF | 3218 bp |
| Locus ID | 21687 |
| Gene Summary | Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1 and TIE1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223075 | Tek (untagged) - Mouse endothelial-specific receptor tyrosine kinase (cDNA clone MGC:59299 IMAGE:6333576), (10ug) |
CNY 12,920.00 |
|
| MR211587 | Tek (Myc-DDK-tagged) - Mouse endothelial-specific receptor tyrosine kinase (cDNA clone MGC:59299 IMAGE:6333576) |
CNY 10,740.00 |
|
| MR211587L3 | Lenti ORF clone of Tek (Myc-DDK-tagged) - Mouse endothelial-specific receptor tyrosine kinase (cDNA clone MGC:59299 IMAGE:6333576) |
CNY 12,640.00 |
|
| MR211587L4 | Lenti ORF clone of Tek (mGFP-tagged) - Mouse endothelial-specific receptor tyrosine kinase (cDNA clone MGC:59299 IMAGE:6333576) |
CNY 12,640.00 |
