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Ube3b (NM_054093) Mouse Tagged ORF Clone
CAT＃: MG211580
- TrueORF®
Ube3b (tGFP-tagged) - Mouse ubiquitin protein ligase E3B (Ube3b)
ORF Plasmid: DDK
"NM_054093" in other vectors (4)
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CNY 11,690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI449831; AU020130 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211580 representing NM_054093
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCACTGTATCTCAGACCTCCAGAGCATGGTTCATCGACAGAGCCCGGCAGGCACGAGAAGAGAGGC TGGTGCAGAAGGAGCGGGAGCGCTCAGCGGTGACGATCCAGGCCCTAGTCCGCAGTTTCCTCTGCCGGAG GCGACTGCACAGAGACATCAGGAAAGAGATTGATGAGTTTTTTAGTGCTGATGAGTCTGGGTCCAGTAAA AGAAGTGCACTTTGTATTTTTAAGATTGCCAGGAGACTGCTGTTTATATGCAAAACTACCGAGGACAGCG AGAGACTGGAGAAGTTGTGTCGAAGCATCCTGAACAGCATGGATGCCGAGAATGAGCCAAAGGTGTGGTA CGTGTCCCTGGCTCTCTCCAAGGACCTCACGCTGCTGTGGATTAAGCAGATCAAGAGCATCCTGTGGCAC TGCTGTGAGCTTCTGGGGCAGCTCAAGCCAGAGATCTTACAGGACTCCAGGCTCATCACGCTGTACCTCA CCATGCTTGTGACCTTCACAGACACTTCAACATGGAAGATTCTCCGGGGAAAAGGTGAGAGCCTTCGGCC AGCCCTGAACCACATTTGCGCAAATATAATGGGCCATCTCAACCAGCGTGGACTTTACTCTGTATTGCAG GTACTGCTGACCCGAGGCCTGGCCAGGCCCCGGCCCTGTCTGTCCAAAGGCATGTTAACAGCAGCCTTCT CTCTGGCCCTGCGCCCTGTGGTTGCTGCGCAGTTCTCAGACAATCTTATGCGACCATTCATCATCCACGT CATGTCGGTACCTGCTCTCGTGGCTCATCTCAGCACCGTGGCCCCTGAGCGCCTCGGCGTTTTAGAGTCC CATGACATGCTTCGTAAATTCATCGTCTTCCTGAGAGATAGAGACCGATGCCGCGATGCTTGTGAGAGTT TAGAAGGCTGCCATACCCTGTGTCTCATGGGCAATCTCCTGCACTTGGGCTCCCTCAGCCTCAGGCTGTT AGAGGAAGAGATGGATGGGTTTGTGAGCGCGCTCACGCAGATGCTGTGCTACTGTCAGAAGTACGTGGCT CAGAAGAAGTCCAACCTGACCCACTGGCACCCTGTGCTCGGCTGGTTTTCCCAACCTGTGGATTACGGCC TCAATGATTCCATGTACCTGATCACCAAGCAGCTGCAGTTCCTGTGGGCGGTGCCTCTCATCCGCATTCT CTTCAGTGACATCCTGAGCAGGAAGCTGCTGGAGCATGCAGAGCCGGCCCCGGTGCAACCGCAGCCGTCC TCACCGCAGACGGTGCTCCCCGTGAAGAGCCTCCTCAAGCGTGCGTTTCAGAAGTCGGCTTCGGTTCGGA ACATCCTCAGGCCTGTTGGGGGCCGCCGTGTCGACTCCGCTGAGGTGCGGAAGGTCTGCAACATTTGCGT GCTCTACCAGACCTCGCTGACGACCCTCACCCAGATCCGCCTGCAGATCCTCACAGGTCTTACGTACCTG GATGACCTGCTGCCCAAGCTCTGGGCGTTTATCTGTGAGCTGGGGCCCCACGGCGGGCTCAAGCTCTTCC TGGAGTGCCTGAACAATGACACTGGAGAGTCCAAGCAGCTCCTGGCCATGCTGATGCTCTTCTGTGACTG TTCCCGGCACCTCATCACCATCCTCGATGACATTGAAGTTTATGAAGAACAGATCTCCTTCAAGCTGGAA GAGCTGGTCACCATCTCCTCGTTCCTCAATTCCTTCGTGTTTAAGATGATCTGGGACGGAATTGTAGAGA ATGCCAAGGGTGAGACCTTGGAGCTGTTCCAGTCCGTCCATGGCTGGCTGATGGTGCTGTATGAGCGAGA CTGCCGCCGGCGCTTTGCTCCGGAGGACCACTGGCTACGGAGGGACCTCAAGCCTGGTGTGCTCTTCCAG GAGCTCGACAAGGACCGAAGGAGGGCTCAGCTGGTCCTGCAGCACATCCCCCACGTCGTGCCTCACAAGA ACAGAGTTCTTCTGTTCCGGAACATGGTTATCAAGGAGAAAGAGAAGCTGGGGCTTGTGGAGACCAGCTC TGCCTCTCCTCACGTCACCCACATCACCATCCGAAGGTCCCGGATGTTGGAGGACGGCTACGAACAGCTC CGGCAGCTTTCCCAGCATGCCATGAAGGGTGTGATCCGAGTGAAGTTTGTCAACGACCTCGGGGTGGACG AGGCTGGGATTGACCAGGATGGTGTCTTCAAGGAGTTTCTGGAAGAGATCATCAAGAGGGTGTTTGACCC CGCACTCAACCTGTTCAAGACAACCAGCGGGGATGAGCGGCTGTACCCATCACCCACGTCCTACATTCAC GAGAATTACCTGCAGCTCTTCGAGTTTGTGGGGAAGATGCTGGGAAAGGCAGTGTATGAGGGGATCGTGG TAGATGTGCCCTTTGCCTCCTTCTTCCTGAGCCAGATGCTCGGGCACCACCACAGCGTCTTCTACAGCTC CGTGGATGAGCTTCCCTCCCTGGACTCGGAGTTTTATAAAAACCTCACCTCCATCAAGCGCTACGATGGA GACATCGCAGACCTCGGGCTGACGCTGTCTTACGATGAAGACGTCATGGGTCAGCTCGTCTGCCACGAAC TGGTTCCCGGAGGGAAGACCATCCCGGTTACAGATGAGAACAAAATTAGCTACATCCATCTGATGGCGCA TTTCCGAATGCACACTCAAATTAAAAACCAGACAGCTGCTCTTATCAGTGGCTTCCGTTCCATCATCAAG CCCGAGTGGATCCGAATGTTCTCTACCCCTGAGCTTCAGCGCCTCATCTCCGGGGACAACGCTGAGATAG ATCTGGAAGACTTAAAGAAGCACACAGTGTACTACGGTGGCTTTCATGGGAGCCACAGGGTCATCATCTG GCTCTGGGATATCCTGGCTTCCGACTTCACGCCTGAAGAAAGAGCCATGTTCCTGAAGTTTGTGACCAGC TGCTCCCGGCCCCCGCTCCTAGGATTCGCCTACCTCAAGCCTCCGTTCTCCATCCGCTGTGTGGAAGTAT CAGATGACCAGGACACTGGGGATACCCTGGGCAGCGTCCTCAGGGGCTTCTTCACCATCCGCAAGAGGGA GCCAGGGGGGCGTCTGCCCACATCTTCCACTTGCTTCAACCTGCTTAAGCTGCCCAACTACAGCAAGAAG AGCGTACTGCGAGAGAAACTACGCTATGCCATCAGCATGAACACGGGCTTTGAGCTCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211580 representing NM_054093
Red=Cloning site Green=Tags(s) MFTVSQTSRAWFIDRARQAREERLVQKERERSAVTIQALVRSFLCRRRLHRDIRKEIDEFFSADESGSSK RSALCIFKIARRLLFICKTTEDSERLEKLCRSILNSMDAENEPKVWYVSLALSKDLTLLWIKQIKSILWH CCELLGQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPALNHICANIMGHLNQRGLYSVLQ VLLTRGLARPRPCLSKGMLTAAFSLALRPVVAAQFSDNLMRPFIIHVMSVPALVAHLSTVAPERLGVLES HDMLRKFIVFLRDRDRCRDACESLEGCHTLCLMGNLLHLGSLSLRLLEEEMDGFVSALTQMLCYCQKYVA QKKSNLTHWHPVLGWFSQPVDYGLNDSMYLITKQLQFLWAVPLIRILFSDILSRKLLEHAEPAPVQPQPS SPQTVLPVKSLLKRAFQKSASVRNILRPVGGRRVDSAEVRKVCNICVLYQTSLTTLTQIRLQILTGLTYL DDLLPKLWAFICELGPHGGLKLFLECLNNDTGESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLE ELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRRDLKPGVLFQ ELDKDRRRAQLVLQHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQL RQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIH ENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDG DIADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIK PEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPEERAMFLKFVTS CSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKK SVLREKLRYAISMNTGFELS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_054093 |
| ORF Size | 3210 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_054093.3 |
| RefSeq Size | 5191 bp |
| RefSeq ORF | 3213 bp |
| Locus ID | 117146 |
| UniProt ID | Q9ES34 |
| Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204494 | Ube3b (untagged) - Mouse ubiquitin protein ligase E3B (Ube3b), (10ug) |
CNY 7,456.00 |
|
| MR211580 | Ube3b (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3B (Ube3b) |
CNY 10,640.00 |
|
| MR211580L3 | Lenti ORF clone of Ube3b (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3B (Ube3b) |
CNY 12,540.00 |
|
| MR211580L4 | Lenti ORF clone of Ube3b (mGFP-tagged) - Mouse ubiquitin protein ligase E3B (Ube3b) |
CNY 12,540.00 |
