Acad10 (BC027825) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2410021P16Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211579 representing BC027825 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGTAAGAAGACTTTTCCAGCCTTCCACTCTGCACTGGGCATGGAGGACCACGGCCCTGAACCATC CCCTGGGCAGGCATCAGGGAGGCCTCCGCTGGACACATTCTGGAGGCCGAAGCTACAGAGCAGTCATTTT TGACACGGGCGGAGTTCTCGTGCCTTCTCCAGGGACAGTGGCTGTGGGCTGGGAGGTGCAGAATCATGTC CCTTCTGGAACTATAGTGAAGGCTTTCATCAGAGGTGGCGACAGTGGGCCCTGGATAAGGTTTATAAAAG GAGAGATAACGACCGAGCACTTCTTGGAAGAATTCGGGAGACTGTGCTCTGAAATCGCAAAGACTTCCGT GCCCGTGAGCTCCTATTTCTCTCTGCTGACCAGTGAGCAGGTGACAAAGCAGTTCCCGGTGATGACTCAG GCCATCTCTCAGATCAGAGCAAAAGGCCTCCAGACTGCAGTCCTCACCAACAACTTTCATCTTTCCAGCG GGGAAAGCTTCCTGCCCCTGGACCGGAAACAGTTTGATGTGGTGGTTGAGTCCTGCCTGGAAGGCATCTG TAAGCCAGATCCCAGGATATTCCAGCTGTGCCTGCAGAGGCTGAGCCTGCAGCCCTCAGAGGCTATTTTT CTTGATGATCTGGGATCCAATCTAAAAGTGGCTGCCAGCCTGGGCATTCACACCATCAAGGTTGACAGGC CCGAGACAGCAGTGAAGGAGCTGGAGGCTCTACTGGGTTTCCCACTGCACTTGGGCGTTCCCAACACCCG GCCTGTGAGGAAGACCATGGCGATTCCACAAGATGCCTTGGAGAAGTACCTGAAGGGCTTGCTGGGGACC CACTCCACCGGCCCCATGGAGCTCCTTCAGTTTGATCATGGACAGTCAAACCCAACGTACTACATCAGGC TGGCTGACCGTCAGCTGGTCCTGAGGAAGAAACCCTCGGGGACCCTCCTCCCCTCTGCCCATGCCATAGA AAGGGAATTCAGGATCATGAAAGCCCTTGCAAATGCAGGAGTCCCTGTCCCTACTGTTCTTGACCTCTGT GAAGACTCAAGCATCATCGGCACCCCCTTCTACTTGATGGAGTACTGCCCGGGCATCATCTACAAAGACC CCTCCCTGCCCGGCTTGGAGCCCAGCCGGCGGGAAGCCATATACACTGCCATGAACCAAGTCCTGTGCAG AATTCACAGTGTGGACCTGCAGGCCACCAGCCTGGACAGCTTCGGCAAGCAAGGGGACTATATTCCTCGA CAGGTGCAAACCTGGACCAAGCAGTACCGAGCAGCAGAAACCAGCAGCATCCCAGCAATGGAGAGGCTTA TCCAGTGGCTGCCACTGCACCTTCCCAGGCAACAGAGAACCACGCTGGTGCATGGTGACTTCAGGCTGGA CAACCTGATATTCCATCCTGAAAAGGCGGAAGTGCTCGCTGTCCTGGACTGGGAACTTTCCACCTTAGGA GACCCCTTTGCTGATGTGGCCTACAGCTGCCTGGCCTACTACCTGCCATCCAGCTTTCCCATTCTGAGAG GGTTCAGAGACCAGGATGTGACAAAGCTGGGCATCCCCACTGTGGAGGAATACTTCAGGATGTACTGTCT TAACATGGGCATCCCACCCATCGACAACTGGAACTTCTACATGGCTTTCTCCTTTTTCCGGGTGGCTGCA ATCCTACAGGGAGTCTACAAGCGGTCACTCACAGGCCAGGCAAGCTCAGCAACAGCCCAACAAAGTGGAA AGCTCACTGAGTCCATGGCTGAGCTGGCCTGGGATTTTGCCACCAAAGAAGGGTTCCGGGTTTTCAAAGA GATGCCAGCCACCAAAACACTGAGCAGATCCTACCATGCCTGGGCGGGACCGAGGTCCCCGAGGACTCCC AAGGGTGTGAGGGGTCACAGCACTGTAGCAGCTGCTTCCCCTTCTCACGAGGCAAAGGGAGGCCTGGTCA TCTCTCCAGAGGGCCTGTCTCCTGCGGTCAGAAAGTTGTATGAGCAGCTGGTGCAGTTTATAGAACAGAA GGTGTACCCCCTGGAGCCAGAGCTGCAGAGACACCAGGCCTCGGCCGACAGGTGGAGCCCTTCCCCGCTG ATAGAAGACCTCAAGGAGAAAGCCAAGGCTGAGGGACTTTGGAACCTTTTCCTACCCTTGGAGACTGATC CTGAGAAAAAGTACGGAGCAGGGCTGACTAACGTGGAATATGCACATCTGTGTGAAGTGATGGGCATGTC TCTGTATGCTTCTGAGATATTTAATTGCTCTGCCCCGGATACGGGGAATATGGAGATCCTGGTGCGGTAT GGCACTGAGGAGCAGAAGGCCCGCTGGCTGGTGCCACTGTTGGAGGGCAGGATCCGTTCTTGCTTTGCCA TGACTGAGCCTCAGGTGGCCTCCTCAGATGCCTCTAACATCGAGGCTTCCATCAAAGAGGAAGATGGCTG CTATGTCATAAATGGTCACAAGTGGTGGACCTCAGGCATCCTGGATCCTCGATGCAAACTCTGTGTGTTT ATGGGGAAAACAGACCCACAGGCCCCAAGGCACCAGCAACAATCTATGCTCTTGGTTCCCATGGACTCGC CAGGAATCACAGTCATCCGGCCTCTGTCAGTGTTTGGTTTGGAGGATCCTCCAGGTGGCCACGGTGAAGT ACGGTTCAAGGACGTGCGTGTGCCCAAGGAAAACATACTCCTGGGCCCTGGCCGAGGCTTTGAGATCGCT CAGGGCAGGCTGGGGCCTGGCCGGATTCATCACTGCATGAGGTTGATTGGGTATTCAGAGAGGGCCCTGG CACTCATGAAGACACGGGTGATGTCCCGCACGGCCTTCGGGAAGCCCCTTGTGGAGCAGGGCACCATCCT GGCAGACATTGCCCGGTCACGTGTGGAGATCGAGCAGGCCCGGCTGCTGGTGCTGAAAGCTGCTCACCTC ATGGATGTGGCCGGAAATAAGACTGCAGCTTTAGACATTGCAATGATTAAAATGGTCGTCCCTTCCATGG CCTATCACGTAATTGACCGCGCTATTCAGGCCTTTGGAGCAGCGGGCCTCAGCAGTGACTACCCCCTGGC TCAGTTCTTTGGCTGGGCCCGAGCCCTGCGCTTCGCTGATGGCCCTGATGAAGTACACCAGCTGACAGTG GCTAAGATGGAACTGAAGAATCAGTCCAGGATGCAGGAACCAGCAGTGCCCAGAGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211579 representing BC027825 Red=Cloning site Green=Tags(s) MLVRRLFQPSTLHWAWRTTALNHPLGRHQGGLRWTHSGGRSYRAVIFDTGGVLVPSPGTVAVGWEVQNHV PSGTIVKAFIRGGDSGPWIRFIKGEITTEHFLEEFGRLCSEIAKTSVPVSSYFSLLTSEQVTKQFPVMTQ AISQIRAKGLQTAVLTNNFHLSSGESFLPLDRKQFDVVVESCLEGICKPDPRIFQLCLQRLSLQPSEAIF LDDLGSNLKVAASLGIHTIKVDRPETAVKELEALLGFPLHLGVPNTRPVRKTMAIPQDALEKYLKGLLGT HSTGPMELLQFDHGQSNPTYYIRLADRQLVLRKKPSGTLLPSAHAIEREFRIMKALANAGVPVPTVLDLC EDSSIIGTPFYLMEYCPGIIYKDPSLPGLEPSRREAIYTAMNQVLCRIHSVDLQATSLDSFGKQGDYIPR QVQTWTKQYRAAETSSIPAMERLIQWLPLHLPRQQRTTLVHGDFRLDNLIFHPEKAEVLAVLDWELSTLG DPFADVAYSCLAYYLPSSFPILRGFRDQDVTKLGIPTVEEYFRMYCLNMGIPPIDNWNFYMAFSFFRVAA ILQGVYKRSLTGQASSATAQQSGKLTESMAELAWDFATKEGFRVFKEMPATKTLSRSYHAWAGPRSPRTP KGVRGHSTVAAASPSHEAKGGLVISPEGLSPAVRKLYEQLVQFIEQKVYPLEPELQRHQASADRWSPSPL IEDLKEKAKAEGLWNLFLPLETDPEKKYGAGLTNVEYAHLCEVMGMSLYASEIFNCSAPDTGNMEILVRY GTEEQKARWLVPLLEGRIRSCFAMTEPQVASSDASNIEASIKEEDGCYVINGHKWWTSGILDPRCKLCVF MGKTDPQAPRHQQQSMLLVPMDSPGITVIRPLSVFGLEDPPGGHGEVRFKDVRVPKENILLGPGRGFEIA QGRLGPGRIHHCMRLIGYSERALALMKTRVMSRTAFGKPLVEQGTILADIARSRVEIEQARLLVLKAAHL MDVAGNKTAALDIAMIKMVVPSMAYHVIDRAIQAFGAAGLSSDYPLAQFFGWARALRFADGPDEVHQLTV AKMELKNQSRMQEPAVPRV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC027825
ORF Size	3209 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027825, AAH27825
RefSeq Size	3619 bp
RefSeq ORF	3209 bp
Locus ID	71985
Gene Summary	Acyl-CoA dehydrogenase only active with R- and S-2-methyl-C15-CoA.[UniProtKB/Swiss-Prot Function]

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