United States
Germany
Japan
United Kingdom
China
Vcl (NM_009502) Mouse Tagged ORF Clone
CAT＃: MG211572
- TrueORF®
Vcl (tGFP-tagged) - Mouse vinculin (Vcl)
ORF Plasmid: DDK
"NM_009502" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9430097D22; AA571387; AI462105; AW545629 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211572 representing NM_009502
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTGTTTCACACGCGTACGATCGAGAGCATCCTGGAGCCGGTGGCGCAGCAGATCTCGCACCTGG TGATTATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTCACCGCGCCCGTAGCCGCCGT GCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGAAAAGAGACTGTTCAGACCACTGAGGATCAGATTCTG AAGAGAGATATGCCACCAGCCTTTATTAAGGTTGAAAATGCTTGCACCAAGCTTGTTCAGGCAGCCCAGA TGCTTCAGTCAGACCCATACTCGGTTCCTGCGCGGGATTACCTCATTGACGGCTCTAGGGGAATCCTTTC TGGCACATCTGACCTACTGCTTACCTTTGATGAGGCTGAGGTTCGTAAAATTATTAGGGTTTGCAAAGGA ATTTTGGAATATCTTACAGTGGCAGAGGTAGTGGAAACTATGGAAGACTTGGTCACTTACACAAAGAATC TTGGGCCAGGAATGACTAAGATGGCCAAAATGATTGATGAGAGACAGCAGGAGTTGACTCACCAGGAACA CCGTGTGATGTTGGTGAACTCAATGAACACTGTCAAAGAGCTGCTTCCTGTTCTCATTTCAGCTATGAAG ATTTTTGTTACAACCAAAAACTCAAAAAACCAAGGAATAGAAGAAGCTTTGAAAAATCGAAATTTTACTG TAGAAAAGATGAGTGCTGAAATTAACGAGATCATTCGTGTGTTACAACTCACTTCCTGGGATGAAGACGC CTGGGCCAGCAAGGACACTGAAGCCATGAAGAGAGCGCTGGCGTCCATAGACTCCAAATTGAACCAGGCC AAAGGTTGGCTCCGTGACCCCAATGCCTCCCCAGGGGATGCTGGAGAGCAGGCCATCAGGCAGATCTTAG ATGAAGCTGGAAAAGTTGGTGAACTTTGTGCAGGCAAGGAACGCAGGGAGATCCTAGGAACCTGCAAAAT GCTAGGGCAGATGACTGACCAAGTGGCTGACCTCCGAGCCAGAGGACAAGGAGCTTCCCCAGTGGCCATG CAGAAGGCCCAGCAAGTGTCTCAGGGGCTCGACGTGCTTACCGCCAAAGTGGAGAATGCAGCTCGGAAGC TGGAAGCCATGACGAACTCAAAGCAGAGCATTGCAAAGAAGATTGATGCTGCCCAGAATTGGCTGGCGGA TCCAAATGGTGGACCTGAGGGAGAAGAACAGATTCGAGGGGCTTTGGCTGAAGCTCGGAAGATTGCAGAA TTATGTGATGATCCTAAGGAGAGAGATGACATCCTCCGCTCCCTTGGAGAGATAGCTGCTCTGACCTCTA AACTAGGAGACTTGCGAAGACAGGGGAAAGGAGACTCGCCAGAGGCTCGAGCCTTGGCTAAACAAGTGGC GACGGCACTACAGAACCTGCAGACCAAAACCAACAGGGCCGTGGCCAACAGCAGACCTGCCAAAGCAGCT GTCCACCTCGAGGGCAAGATTGAACAGGCGCAGCGGTGGATTGATAACCCCACAGTAGATGACCGTGGAG TCGGTCAGGCTGCCATCCGTGGACTTGTGGCTGAGGGGCATCGGCTGGCCAATGTCATGATGGGACCTTA TCGCCAAGATCTTCTTGCCAAATGTGACCGTGTAGACCAGCTAACAGCTCAGCTGGCTGACCTGGCTGCC CGAGGGGAGGGGGAGAGTCCTCAGGCGAGAGCACTTGCATCCCAGCTTCAGGACTCCTTAAAGGATCTTA AAGCCCAGATGCAGGAAGCTATGACTCAAGAGGTATCCGATGTTTTCAGCGATACTACAACTCCTATCAA GCTGTTGGCAGTAGCCGCCACTGCTCCTCCTGATGCACCCAATAGGGAAGAGGTATTTGATGAAAGGGCA GCCAATTTTGAAAACCATTCAGGAAGGCTTGGAGCCACAGCAGAGAAGGCGGCTGCTGTTGGTACTGCCA ATAAATCGACAGTGGAAGGCATTCAGGCATCTGTGAAGACAGCCCGAGAACTCACTCCCCAGGTCATCTC CGCTGCTCGGATCTTACTGAGGAACCCTGGTAACCAGGCTGCTTATGAACATTTTGAGACCATGAAGAAC CAGTGGATTGATAATGTTGAAAAAATGACAGGGCTGGTGGACGAGGCTATTGATACCAAGTCTCTGTTGG ATGCTTCTGAAGAAGCAATTAAAAAAGACCTGGACAAGTGTAAGGTAGCCATGGCCAATATTCAGCCTCA GATGCTGGTCGCTGGAGCAACCAGTATTGCTCGTCGGGCCAACCGGATTCTGCTGGTTGCTAAGAGGGAG GTAGAGAACTCTGAGGACCCGAAGTTCCGAGAGGCTGTGAAAGCTGCCTCTGATGAACTGAGCAAAACAA TCTCCCCCATGGTGATGGATGCCAAGGCTGTGGCTGGAAACATCTCTGACCCTGGCCTGCAAAAGAGCTT CCTGGACTCAGGATATCGGATCCTGGGAGCTGTGGCCAAGGTCAGAGAAGCCTTCCAACCTCAGGAGCCT GACTTCCCGCCTCCTCCACCAGACCTTGAACAGCTACGACTAACTGATGAGCTGGCTCCTCCTAAGCCAC CTCTGCCTGAGGGTGAAGTCCCTCCACCCAGGCCCCCACCACCAGAAGAGAAGGATGAAGAGTTCCCTGA GCAGAAAGCTGGTGAGGTGATTAACCAGCCAATGATGATGGCCGCCAGGCAGCTCCACGATGAAGCTCGG AAATGGTCTAGCAAGGGCAATGACATCATTGCAGCAGCCAAGCGCATGGCTCTGCTGATGGCAGAGATGT CTCGGCTGGTAAGAGGGGGCAGTGGTACCAAGCGGGCACTTATTCAGTGTGCCAAGGATATCGCCAAGGC CTCTGATGAGGTGACGAGGTTGGCCAAGGAGGTTGCCAAGCAGTGCACAGATAAGCGGATTAGAACCAAT CTCTTACAGGTATGCGAGCGAATCCCAACTATAAGCACCCAGCTCAAAATCCTATCCACAGTGAAGGCCA CTATGCTGGGCCGGACCAACATCAGTGATGAGGAGTCTGAGCAGGCCACAGAGATGCTGGTTCATAATGC CCAGAACCTCATGCAGTCTGTGAAGGAGACTGTGCGAGAGGCTGAAGCTGCTTCAATCAAAATCCGAACA GATGCTGGCTTTACTCTGCGCTGGGTCAGAAAGACTCCCTGGTACCAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211572 representing NM_009502
Red=Cloning site Green=Tags(s) MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQIL KRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKG ILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMK IFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQA KGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAM QKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAE LCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAA VHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAA RGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERA ANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKN QWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKRE VENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEP DFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEAR KWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTN LLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRT DAGFTLRWVRKTPWYQ SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009502 |
| ORF Size | 3198 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009502.3 |
| RefSeq Size | 5229 bp |
| RefSeq ORF | 3201 bp |
| Locus ID | 22330 |
| UniProt ID | Q64727 |
| Gene Summary | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223409 | Vcl (untagged) - Mouse vinculin (Vcl), (10ug) |
CNY 13,680.00 |
|
| MR211572 | Vcl (Myc-DDK-tagged) - Mouse vinculin (Vcl) |
CNY 7,424.00 |
|
| MR211572L3 | Lenti ORF clone of Vcl (Myc-DDK-tagged) - Mouse vinculin (Vcl) |
CNY 10,260.00 |
|
| MR211572L4 | Lenti ORF clone of Vcl (mGFP-tagged) - Mouse vinculin (Vcl) |
CNY 10,260.00 |
