Llgl1 (BC055399) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Mgl1, Llglh
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211565 representing BC055399 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAAGTTTCGGTTCCGGCGGCAGGGCGCCGACCCGCAGCGCGAGAAGCTTAAGCAGGAGCTCTTCG CCTTCCACAAGACTGTGGAGCATGGCTTCCCCAATCAGCCCAGCGCCTTGGCCTTCGATCCTGAGCTCCG CATCATGGCCATCGGCACCAGATCTGGGGCCGTCAAGATCTATGGTGCACCCGGAGTGGAATTTACAGGC CTACATCGGGACGCAGCCACCGTCACCCAGATGCATTTCCTCCCTGGTCAGGGCCGCCTCCTGACCCTGC TAGATGACAGCAGCTTGCATCTCTGGGAGATCATCCATCATAATGGCTGCGCCCACCTGGAGGAAGGCCT CAGCTTCCACCCACCCAGCAGACCCAGTTTTGACAATGCCAGTTTCCCTGCCAGTCTAACACGTGTCACT GTGGTCCTGCTCGTAGCTGGCAATACAGCAGCCCTGGGAACTGAGAGTGGTAGCATATTCTTCCTGGATG TAGCCACCCTGGCACTGCTGGAGGGGCAGACTCTCAGCCCAGATGTGGTTCTGCGCAGTGTGCCAGATGA TTACCGGTGTGGAAAGGCCTTGGGCCCTGTGGAGTCACTCCAGGGACATCTGCAAGACCCCAGCAAGATC CTCATAGGCTACAGTCGGGGTTTACTGGTCATCTGGAGCCAGGCCACACAGTCTGTGGACAACGTTTTCC TAGGTAACCAGCAGCTGGAGAGCCTGTGTTGGGGCCGTGATGGCAGCAGCATTATCAGCTCACACAGTGA TGGCAGCTATGCCATCTGGTCCACAGACACTGGCAGCCCCCCAACGCTGCAGCCCACTGTAGTGACCACA CCCTACGGCCCCTTCCCCTGCAAGGCCATCAACAAGATTCTGTGGCGGAGCTGTGAGTCAGGAGACCACT TTATCATCTTCAGTGGTGGCATGCCTCGAGCCAGCTATGGTGACCGCCACTGTGTGAGTGTACTGCGAGC AGAGACACTGGTGACCCTGGACTTCACCTCTCGTGTCATTGACTTCTTCACGGTGCACAGCACACAGCCA GAGGATGAGTGTGACAACCCCCAGGCCTTAGCCGTGCTTCTGGAGGAGGAGCTGGTGGTGCTGGACCTGC AGACACCAGGCTGGCCAGCTGTGCCCGCCCCTTACCTGGCCCCACTGCATTCGTCAGCTATCACCTGCTC TGCCCATGTTGCCAATGTCCCCAGCAAGCTGTGGGCCCGCATTGTAAGTGCTGGTGAGCAGCAGAGCCCA CAGCCTGCCTCCAGTGCCTTGAGTTGGCCCATTACCGGGGGCCGGAACTTGGCCCAGGAACCCTCGCAGC GTGGGCTGCTGCTCACTGGCCATGAGGATGGCACTGTGCGGTTCTGGGACGCCTCTGGTGTGGCGCTAAG GCCACTCTACAAACTGAGCACAGCTGGCCTCTTCCAGACGGACTGTGAACATGCTGACAGCCTGGCCCAG GCTGTGGAGGATGACTGGCCGCCCTTCCGCAAGGTGGGCTGCTTTGATCCCTACAGTGATGACCCCCGGC TAGGAATCCAGAAGGTTGCGCTTTGCAAGTACACAGCCCAGATGGTGGTAGCTGGCACTGCAGGCCAGGT GCTGGTGCTGGAGCTCAGTGAGGTCCCAGCAGAGCATGCCGTCAGTGTGGCCAACGTGGATCTTCTTCAG GATCGCGAGGGCTTCACGTGGAAGGGTCACGAGCGGCTGAACCCACACACGGGGCTGCTGCCGTGGCCTG CCGGATTCCAGCCCCGCATGCTGATACAGTGCCTCCCACCCGCCGCTGTCACTGCTGTCACACTCCATGC TGAGTGGAGCCTCGTGGCCTTTGGTACCAGTCATGGCTTTGGCCTTTTTGACTACCAGCGCAAGAGCCCT GTGCTGGCTAGGTGCACCCTTCACCCCAATGATTCTTTGGCCATGGAGGGGCCACTGTCACGGGTGAAGT CCCTCAAGAAGTCACTGAGACAGTCATTCCGGCGAATCCGCAAGAGCCGTGTCTCAGGCAAAAAACGGAC TCCTGCTGCCAGTAGCAAGTTGCAGGAGGCCAATGCCCAGCTGGCCGAGCAGACCTGCCCACACGACCTG GAGATGACACCCGTGCAGCGCCGCATTGAGCCTCGGTCTGCTGACGACTCGCTCTCCGGTGTTGTACGCT GCCTCTACTTTGCTGACACGTTCCTTCGAGATGCGACCCACCACGGGCCCACCATGTGGGCGGGCACCAA CTCGGGCTCTGTGTTCGCCTATGCGCTGGAGGTTCCAGCAGCCACAGCAGGCGGAGAGAAGCGGCCTGAG CAGGCAGTGGAGGCGGTGCTGGGCAAGGAGGTGCAGCTAATGCACCGAGCACCTGTGGTGGCCATTGCTG TGCTGGATGGTCGTGGCCGGCCACTGCCTGAGCCCTATGAGGCTTCCCGGGACCTGGCCCAGGCGCCAGA CATGCAAGGCGGCCATGCTGTGCTCATTGCATCTGAGGAACAATTCAAGGTGTTCACACTACCCAAGGTG AGTGCTAAGACTAAATTCAAGCTTACAGCCCATGAAGGCTGTCGTGTGCGGAAGGTAGCCCTGGCTACAT TTGCCAGCGTGATGTCTGAGGACTATGCCGAGACCTGCCTTGCCTGCCTCACCAACCTGGGTGATGTCCA CGTCTTCTCGGTGCCTGGCCTGAGGCCTCAGGTGCACTACTCCTGTATCCGGAAGGAGGACATCAGTGGC ATCGCTTCCTGTGTCTTCACACGTCACGGCCAGGGCTTTTACTTGATTTCTCCATCGGAATTTGAGCGCT TCTCACTGAGTGCTCGCAACATCACAGAACCACTATGTTCTCTGGATATAAGCTGGCCCCAAAATGCCAC CCAGCCCAGGCTTCAAGAGTCACCCAAGCTGAGCCAGGCTAATGGGACCAGAGACATCATTCTGGCCCCA GAGAGCTGCGAAGGAAGCCCTAGCTCTGCCCACAGCAAGCGAGCTGATACCATGGAACCACCCGAGGCCG CTCTCTCGCCTGTGTCCATTGATTCAGCTGCTAGTGGGGATACCATGCTGGACACAACAGGGGATGTCAC CGTGGAATATGTGAAGGATTTTCTGGGCTCTCCTGAGGACTCTGAGAAGAACCTGCGGAACCTGGAGGCA GATGATGCTTGTCGGGCCTACACCCTTCTGATTAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211565 representing BC055399 Red=Cloning site Green=Tags(s) MMKFRFRRQGADPQREKLKQELFAFHKTVEHGFPNQPSALAFDPELRIMAIGTRSGAVKIYGAPGVEFTG LHRDAATVTQMHFLPGQGRLLTLLDDSSLHLWEIIHHNGCAHLEEGLSFHPPSRPSFDNASFPASLTRVT VVLLVAGNTAALGTESGSIFFLDVATLALLEGQTLSPDVVLRSVPDDYRCGKALGPVESLQGHLQDPSKI LIGYSRGLLVIWSQATQSVDNVFLGNQQLESLCWGRDGSSIISSHSDGSYAIWSTDTGSPPTLQPTVVTT PYGPFPCKAINKILWRSCESGDHFIIFSGGMPRASYGDRHCVSVLRAETLVTLDFTSRVIDFFTVHSTQP EDECDNPQALAVLLEEELVVLDLQTPGWPAVPAPYLAPLHSSAITCSAHVANVPSKLWARIVSAGEQQSP QPASSALSWPITGGRNLAQEPSQRGLLLTGHEDGTVRFWDASGVALRPLYKLSTAGLFQTDCEHADSLAQ AVEDDWPPFRKVGCFDPYSDDPRLGIQKVALCKYTAQMVVAGTAGQVLVLELSEVPAEHAVSVANVDLLQ DREGFTWKGHERLNPHTGLLPWPAGFQPRMLIQCLPPAAVTAVTLHAEWSLVAFGTSHGFGLFDYQRKSP VLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRTPAASSKLQEANAQLAEQTCPHDL EMTPVQRRIEPRSADDSLSGVVRCLYFADTFLRDATHHGPTMWAGTNSGSVFAYALEVPAATAGGEKRPE QAVEAVLGKEVQLMHRAPVVAIAVLDGRGRPLPEPYEASRDLAQAPDMQGGHAVLIASEEQFKVFTLPKV SAKTKFKLTAHEGCRVRKVALATFASVMSEDYAETCLACLTNLGDVHVFSVPGLRPQVHYSCIRKEDISG IASCVFTRHGQGFYLISPSEFERFSLSARNITEPLCSLDISWPQNATQPRLQESPKLSQANGTRDIILAP ESCEGSPSSAHSKRADTMEPPEAALSPVSIDSAASGDTMLDTTGDVTVEYVKDFLGSPEDSEKNLRNLEA DDACRAYTLLIK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC055399
ORF Size	3188 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC055399, AAH55399
RefSeq Size	4320 bp
RefSeq ORF	3188 bp
Locus ID	16897
Gene Summary	Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells. Involved in axonogenesis through RAB10 activation thereby regulating vesicular membrane trafficking toward the axonal plasma membrane.[UniProtKB/Swiss-Prot Function]

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