Sec16b (BC059194) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Lztr2; Rgpr; Rgpr-p117
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211534 representing BC059194 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCTTGGGTTCCCCACACACAAGGAAGGACCACGGGACCATCAAGGGATACAAATAGAGGACTTC AGAGTGGACATTATAGACCCCGTCCGCATTCTCAGTACAGTGGAGATAAGTACCACCAATGGCAAGATGC CCACAAGAACTCAAAGTCACAGCAGGACCTCAGGGATGACCACCAACAGTCTCACTCTGTATCCAGGAGT GGGGAGTGGTCCCAGCCTGTGTCTGGTGCTGACTACTTGAAAGGATCTTATCCCAGTCACCTGTACTCAA GGTCAGGCTATGGGGACCCCTATCAGAGGTACCACACTCCGACACCGAGGGATGAGTATGCTTATGGAAA TTACTACTACCACGGACATCCACAGCTGCTGCCGGAAGAAAGAGTGGCAAGGCAAGGGAGTCCTTATATC TGGCATGAAGATCATGGAGACCAGAGGTACTTCGGCGAGCATCATCGGGAGAAGCACAATGGTACATTTG GAGCAAACAGTGATACCCAGTTCCAATTTACCAGTAAGAATCCATACCGAGACAGCCCTGCTTCTGTGTC TGGACAGGAACAGCCTGGGGAATTCTTTCCAGAGAGTGAAGCCCAGAAACAAAAGCCATTGCTGACCAGC AAATCCAGCCTTCTCCAGCAGCACGAGTCTGGGCTCAGCTCTAGTAGCTACGAGCTCAGTCAGTACATGA CAGCTGCCCCCGAGGAGTATGAACCTATGGTGTCAGCAGCTTGGAGGCCTATTCAGGCTGATGACACCTC AGCGACAGTTCCAAAGGCACCTATGAGGTTCTATGTTCCCCATGTGTCTGTGAGCTTTGGGCCAGGAGGC CAGCTAGTGTGTGTCCCCCCCAACTCTCCTGCTGATGGACAAACGGCCCTCGTTGAAGTGCACAGCATGG AGGTTTTACTTAATGATTTTGAGGATCAGGAGGAGATGAGAGCTTTCCCAGGGCCCCTTATTAGGGAGGA CATACACAAGGTGGATATCATGACGTTTTGTCAGCAGAAAGCAACGCAGTGTCTCAAATCTGAGACACCA GGGAGCAGAGACTCAGCTCTACTTTGGCAACTGCTGGTTCTCCTTTGTCGACAGAATGGGTCCATGGTGG GATCTGACATTGCTGAGCTGCTGATGCAGGACTGCAAGAAGCTGGAGAAGTACAAGAGGCAGCCCCCTGT AGCCAACCTCATCAACCTGACTGACGAGGACTGGCCGGTACTGAGCTCTGGGACTCGTGATCTCCTCACT GGGGAGATTCCCCCCAATGTGGATACGCCAGCGCAGATCGTGGAGAAATTCACCAAGCTGCTCTACTACG GAAGGAAGAAGGAGGCTTTGGAGTGGGCTATGAAGAACCACTTGTGGGGACATGCCTTGTTCCTAGCCAG TAAGATGGACCCAAGGACCTACAACTGGGTCATGAGTGGTTTCACCAGTACGCTGGCGCTCAATGACCCT CTGCAGACCCTCTTCCAGCTCATGTCAGGAAGGATTCCACAAGCAGCCACGGTTTGTGGAGACAAACAGT GGGGAGACTGGCGGCCCCACTTGGCTGTGATCCTGTCAAATCAGGCTGGAGATACAGAGTTGTACCAGCG AGCGATTGTCAGCATGGGGGATACCCTGGCTGGAAAAGGGCTTGTGGAGGCATCTCACTTCTGCTATCTC ATGGCTCATGTGCCCTTTGGTCACTATACGGTGAAGACAGATCATCTGGCCTTGGTGGGCAGTAGCCACA GTCAAGAGTTTATGAAATTTGCAACAATTGAGGCTATCCAGCGGACGGAAATCTTCGAGTACTGCCAGAT GCTGGGCCGCCCCAAATCCTTCATCCCTTCTTTCCAGGTGTATAAGCTCCTCTATGCCTCCCGTCTGGCA GATTATGGCCTGGCATCCCAGGCCTTGCATTACTGTGAAGCCATTGGTGCAGCTGTCTTGAGCCAGGAAG GGAGCAGCCACCCTGTGCTATTAGCGGAGCTCATCAAGCTCGCAGAGAAACTGAAGCTGTCAGATCCTCT GGTTTTAGAAAGACGCCGTGGGGACAGGGACCTGGAACCAGATTGGCTTGTGCAACTGCGGAGGAAGCAC AAGGACCTGGAGCAAAATAGAACAGGAGCCCTGAGAGATCCTGATTCGACTCCTTCAGATATTTATGGAG CTGGAGGAACAACAGACACTCCCTACCCTGATCTCTCTGGCCATCAAAACTACTCAGAAGACTCTGAGTA CAGCTCCACCTTGTGGTCAACAGCAGAGCAGACAAGCCTGACCAATCCCCTCGCACAGCAGTCTTTCCCC CTGCAGCGAGATACCTATTCAGGACACATGGGAACCCCAGTGCCTCTCTACTCAGTTCCTGCAACCCACT TGGCAGTGACGAGTGGAGCCAGTGGTAGCAGTGTGGCAGTGACGGGGACTCCTGGGGGAAGGGTTGGAGA GGATATGCTGCGGACACATCCTGCCTTTGGAGAGAACACAATGACTCAAGAACCCCTGGAAGATCCTGAT GGTCTCGAAGTCATTTCCAGTCTTCAGACACCAGCAGCTCCCAGGGTTCCAAGTTTTTCTGAGGATTCTG CTGCTTCAGCCAAGGAGGATGAGGAGGGGTCTTCTGATGGAGCAGACAAACCATCTCACCCCGATGCTTC ACAGAAAGGAAAGCTGGGAGATGGGAAGAATACAAAGAGCTCGGGATTCGGCTGGTTCAGCTGGTTTCGA TCAAAGCCTGCCAGCAGTGTGTCCACCTCTGGAGATGAAGACTCCTCGGACAGTTCTGACTCAGAGGAGT CTCCCAGAGCATCTCCTCCTCACCATGCGAGCCCAGGCCTCTCACCAACACCTCCTCTCACATCCCCATC CCTGCCAGGTGCCAGCACCTTCTCGAGAGGCACAGGTGGGAGTATTCTCCAAGGATCCTCAAACAGTAGT GGAATCGCTGAAGGCATGGGGATTGGAGGGTTCTCGGGGACACAGGGTGTTTCTTCTGAGTTCTACTCCC AGCCTGGTGCCCTGCCTCCTCCGCCTACCTTGCAAGGTGCTGTTCCTCTCTACAATCCATCTCAGGTCCC TCAGCTTCCCACTGCTAGTAGCCTGAACCGGCCAAACCGCCTAGCCCAGCGCCGCTACCCAACCCAGCCA TGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211534 representing BC059194 Red=Cloning site Green=Tags(s) MEPWVPHTQGRTTGPSRDTNRGLQSGHYRPRPHSQYSGDKYHQWQDAHKNSKSQQDLRDDHQQSHSVSRS GEWSQPVSGADYLKGSYPSHLYSRSGYGDPYQRYHTPTPRDEYAYGNYYYHGHPQLLPEERVARQGSPYI WHEDHGDQRYFGEHHREKHNGTFGANSDTQFQFTSKNPYRDSPASVSGQEQPGEFFPESEAQKQKPLLTS KSSLLQQHESGLSSSSYELSQYMTAAPEEYEPMVSAAWRPIQADDTSATVPKAPMRFYVPHVSVSFGPGG QLVCVPPNSPADGQTALVEVHSMEVLLNDFEDQEEMRAFPGPLIREDIHKVDIMTFCQQKATQCLKSETP GSRDSALLWQLLVLLCRQNGSMVGSDIAELLMQDCKKLEKYKRQPPVANLINLTDEDWPVLSSGTRDLLT GEIPPNVDTPAQIVEKFTKLLYYGRKKEALEWAMKNHLWGHALFLASKMDPRTYNWVMSGFTSTLALNDP LQTLFQLMSGRIPQAATVCGDKQWGDWRPHLAVILSNQAGDTELYQRAIVSMGDTLAGKGLVEASHFCYL MAHVPFGHYTVKTDHLALVGSSHSQEFMKFATIEAIQRTEIFEYCQMLGRPKSFIPSFQVYKLLYASRLA DYGLASQALHYCEAIGAAVLSQEGSSHPVLLAELIKLAEKLKLSDPLVLERRRGDRDLEPDWLVQLRRKH KDLEQNRTGALRDPDSTPSDIYGAGGTTDTPYPDLSGHQNYSEDSEYSSTLWSTAEQTSLTNPLAQQSFP LQRDTYSGHMGTPVPLYSVPATHLAVTSGASGSSVAVTGTPGGRVGEDMLRTHPAFGENTMTQEPLEDPD GLEVISSLQTPAAPRVPSFSEDSAASAKEDEEGSSDGADKPSHPDASQKGKLGDGKNTKSSGFGWFSWFR SKPASSVSTSGDEDSSDSSDSEESPRASPPHHASPGLSPTPPLTSPSLPGASTFSRGTGGSILQGSSNSS GIAEGMGIGGFSGTQGVSSEFYSQPGALPPPPTLQGAVPLYNPSQVPQLPTASSLNRPNRLAQRRYPTQP C TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC059194
ORF Size	3155 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC059194, AAH59194
RefSeq Size	4440 bp
RefSeq ORF	3155 bp
Locus ID	89867
Gene Summary	Plays a role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus. Involved in peroxisome biogenesis. Regulates the transport of peroxisomal biogenesis factors PEX3 and PEX16 from the ER to peroxisomes.[UniProtKB/Swiss-Prot Function]

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