Ank2 (BC059251) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	ankyrin>B<, Ankyrin-2, Ankyrin-B, ankyrin B
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211532 representing BC059251 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGAGGTCCTTGATGTTTCTGATGAAGAGGGTGATGACACTGTGACAGGTGATGGGGGAGAATACC TCAGGCCAGAAGATCTCAAGGAGCTCGGAGATGACTCACTGCCCAGCAGTCAGTTCCTGGATGGCATGAA CTACCTTCGGTACAGTCTGGAGGGAGGAAGATCCGACAGCCTCCGGTCCTTCAGTTCCGACAGGTCTCAC ACTCTGAGCCATGCATCGTACCTGAGGGACAGTGCCATGATTGACGACACGGTTGTGATCCCCAGCCACC AGGTGTCTGCGCTAGCCAAGGAGGCAGAAAGGAATTCTTATCGTCTGAGCTGGGGCACTGAGAACTTAGA CAACGTGGCTCTTTCTTCCAGTCCTATTCATTCAGGTTTCCTAGTCAGTTTTATGGTGGATGCCCGTGGT GGTGCCATGCGAGGATGCAGACACAATGGACTCAGAATCATTATCCCACCTCGGAAATGCACAGCCCCAA CTCGCGTCACCTGCCGCCTTGTGAAACGCCATCGACTGGCAACAATGCCGCCCATGGTGGAAGGAGAAGG CCTGGCCAGCCGCCTGATTGAAGTCGGACCTTCGGGAGCTCAGTTTCTTGGGCCCGTGATCGTGGAGATC CCTCACTTCGCCGCTCTTCGAGGAAAGGAGAGGGAGCTGGTGGTCCTGCGCAGTGAAAATGGGGACAGCT GGAAAGAGCATTTCTGCGACTACACTGAGGATGAATTGAACGAAATCCTTAATGGCATGGATGAAGTGCT GGACAGTCCAGAAGACCTGGAAAAGAAACGAATCTGCCGCATCATCACTCGTGACTTCCCACAGTACTTT GCGGTGGTGTCTCGCATCAAACAGGACAGCAACCTGATTGGCCCCGAGGGTGGAGTACTGAGCAGCACAG TGGTGTCACAGGTGCAAGCCGTCTTCCCAGAGGGCGCACTCACCAAGCGCATTCGAGTAGGCCTACAGGC TCAACCTATGCATAGTGAATTGGTAAAGAAGATCTTAGGCAACAAAGCCACCTTCAGCCCAATAGTCACT TTGGAACCCAGGAGAAGAAAATTCCACAAGCCAATTACTATGACTATTCCTGTCCCCAAAGCTTCAAGTG ATGTCATGCTGAATGGTTTTGGGGGAGACGCACCAACCTTAAGATTACTGTGCAGCATAACAGGTGGAAC TACGCCTGCCCAGTGGGAAGACATCACAGGAACAACGCCACTAACATTTGTCAATGAGTGTGTTTCCTTT ACAACCAACGTGTCTGCCAGGTTCTGGCTGATAGACTGTCGACAGATTCAGGAGTCTGTTGCCTTTGCAT CACAAGTGTATAGAGAAATTATCTGTGTGCCATATATGGCCAAATTTGTAGTGTTTGCCAAGTCACATGA CCCCATTGAAGCCAGGTTGCGGTGTTTCTGTATGACAGATGACAAAGTAGATAAGACCCTTGAACAACAA GAAAACTTCTCTGAGGTGGCCAGGAGCAGGGATGTGGAGGTATTGGAAGGAAAACCTATTTATGTTGATT GTTTTGGCAACCTGGTTCCACTAACCAAGAGTGGCCAACATCATATATTCAGCTTTTTTGCCTTCAAAGA AAATAGACTTCCTCTCTTTGTCAAGGTTCGTGACACAACCCAGGAACCTTGCGGGCGACTCTCATTTATG AAGGAACCCAAATCCACAAGAGGACTGGTGCATCAAGCTATTTGCAACTTAAACATCACCCTGCCAATTT ATGCCAAGGAATCGGAGTCAGATCAAGAGCCGGAGGAAGAGATCGGTATGACATCCGAAAAAAATCCCCA AGATGAGCAGGAGCGGATGGAAGAAAGGCTGGCTTACATCGCTGATCACCTTGGCTTCAGCTGGACAGAA TTAGCAAGAGAACTGGATTTCACTGAGGAGCAAATTCACCAAATTCGAATCGAGAACCCCAACTCCCTTC AAGATCAGAGCCACGCACTGCTCAAGTACTGGCTGGAGAGGGATGGGAAGCATGCCACAGATACCATCCT CATCGAATGCCTCACCAAGATCAACAGGATGGACATTGTACATCTCCTGGAGACCAACACAGAGCCCCTC CAGGAGCGCATGGGCCGCAGCTATGCAGAAATAGAGCAGACCATTACGCTGGACCACAGTGAAGGATTTT CAGTCCTTCCAGACGAGCTCTGTGCTGCCAAGGAGAAGAAGGAGCAGGAAGCTTCCAAAGAAAGCGAGTC TAGCGACCACCCGCCCATGGTCTCCGAAGAAGACATATCTGTCGGTTATTCCACATTTCAGGATTGCCTC CCCAAAACTGAAGGGGACAGCCCAGCAGCAGCACTGTCTCCTCAAATGCACCAGGAGCCAGTTCAACAAG ATTTCTCAGGGAAAACGCAAGACCAGCAGGAATATTATGTAACAACACCAGGGGCAGAAGTGGAAGACCC TCAGAAGGCCACAGCCGTTCCTGACTCTCTCTGTAAGACTCCTGAGGACATCAGTACCCCTCCTGAGGGA ACAAAGCCTTGTCTCCAGACCCCGGTGACTAGCGAACGTGGTTCTCCGATTGTGCAAGAACCTGAGGAGG CTTCCGAGCCCAAAGAGGAGAGTTCTCCAAGGAAAACTAGCCTGGTCATTGTTGAGTCAACAGACGACCA GTCTCAGGTCTTTGAAAGACTGGATGGTGATGCCGCTTTTCAAAAGGGAGACGATATGCCTGACATACCC CCAGAGACGGTCACAGAGGAAGAATATGTTGATGAGAATGGACACACCGTGGTGAAGAAGGTTACCCGGA AAATCATTAGGCGGTACGTTTCCTCTGATGGCACAGAGAAGGAGGAGGTTACCATGCAGGGAATGCCTCA GGAGCCAGTCAACATTGAGGATGGGGACAATTATTCCAAAGTGATAAAGCGCGTGGTATTGAAGAGTGAC ACCCAGCAGTCAGAGGTGACTTTGTCTGAACCCAGCGTTTTGTCCAGTACCTCACAGTTTCAGGCCGAGC CAGTAGAAGGCCGTAGAGTCAGCAAAGTTGTTAAAACAACCATGGTACACGGAGAACGGATGGAGAAGAG TCTTGGGGACTCTAGCTTAGCCACTGACCTTCCTTCAGCCAAAGATGACTTTGAAGAGGACAACAATGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211532 representing BC059251 Red=Cloning site Green=Tags(s) MTEVLDVSDEEGDDTVTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSH TLSHASYLRDSAMIDDTVVIPSHQVSALAKEAERNSYRLSWGTENLDNVALSSSPIHSGFLVSFMVDARG GAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEI PHFAALRGKERELVVLRSENGDSWKEHFCDYTEDELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYF AVVSRIKQDSNLIGPEGGVLSSTVVSQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVT LEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSF TTNVSARFWLIDCRQIQESVAFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQ ENFSEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFM KEPKSTRGLVHQAICNLNITLPIYAKESESDQEPEEEIGMTSEKNPQDEQERMEERLAYIADHLGFSWTE LARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTILIECLTKINRMDIVHLLETNTEPL QERMGRSYAEIEQTITLDHSEGFSVLPDELCAAKEKKEQEASKESESSDHPPMVSEEDISVGYSTFQDCL PKTEGDSPAAALSPQMHQEPVQQDFSGKTQDQQEYYVTTPGAEVEDPQKATAVPDSLCKTPEDISTPPEG TKPCLQTPVTSERGSPIVQEPEEASEPKEESSPRKTSLVIVESTDDQSQVFERLDGDAAFQKGDDMPDIP PETVTEEEYVDENGHTVVKKVTRKIIRRYVSSDGTEKEEVTMQGMPQEPVNIEDGDNYSKVIKRVVLKSD TQQSEVTLSEPSVLSSTSQFQAEPVEGRRVSKVVKTTMVHGERMEKSLGDSSLATDLPSAKDDFEEDNNE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC059251
ORF Size	3152 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC059251, AAH59251
RefSeq Size	4997 bp
RefSeq ORF	3152 bp
Locus ID	109676
Gene Summary	Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions (PubMed:19109891). In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites (PubMed:12571597). Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:15262991). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (PubMed:19007774). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (PubMed:27718357).[UniProtKB/Swiss-Prot Function]

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