Ank2 (BC059251) Mouse Tagged ORF Clone

CAT#: MG211532

  • TrueORF®

Ank2 (tGFP-tagged) - Mouse ankyrin 2, brain (cDNA clone MGC:67574 IMAGE:6405358)



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Mouse Protein / Lysate Request

CNY 9,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms ankyrin>B<, Ankyrin-2, Ankyrin-B, ankyrin B
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211532 representing BC059251
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGAGGTCCTTGATGTTTCTGATGAAGAGGGTGATGACACTGTGACAGGTGATGGGGGAGAATACC
TCAGGCCAGAAGATCTCAAGGAGCTCGGAGATGACTCACTGCCCAGCAGTCAGTTCCTGGATGGCATGAA
CTACCTTCGGTACAGTCTGGAGGGAGGAAGATCCGACAGCCTCCGGTCCTTCAGTTCCGACAGGTCTCAC
ACTCTGAGCCATGCATCGTACCTGAGGGACAGTGCCATGATTGACGACACGGTTGTGATCCCCAGCCACC
AGGTGTCTGCGCTAGCCAAGGAGGCAGAAAGGAATTCTTATCGTCTGAGCTGGGGCACTGAGAACTTAGA
CAACGTGGCTCTTTCTTCCAGTCCTATTCATTCAGGTTTCCTAGTCAGTTTTATGGTGGATGCCCGTGGT
GGTGCCATGCGAGGATGCAGACACAATGGACTCAGAATCATTATCCCACCTCGGAAATGCACAGCCCCAA
CTCGCGTCACCTGCCGCCTTGTGAAACGCCATCGACTGGCAACAATGCCGCCCATGGTGGAAGGAGAAGG
CCTGGCCAGCCGCCTGATTGAAGTCGGACCTTCGGGAGCTCAGTTTCTTGGGCCCGTGATCGTGGAGATC
CCTCACTTCGCCGCTCTTCGAGGAAAGGAGAGGGAGCTGGTGGTCCTGCGCAGTGAAAATGGGGACAGCT
GGAAAGAGCATTTCTGCGACTACACTGAGGATGAATTGAACGAAATCCTTAATGGCATGGATGAAGTGCT
GGACAGTCCAGAAGACCTGGAAAAGAAACGAATCTGCCGCATCATCACTCGTGACTTCCCACAGTACTTT
GCGGTGGTGTCTCGCATCAAACAGGACAGCAACCTGATTGGCCCCGAGGGTGGAGTACTGAGCAGCACAG
TGGTGTCACAGGTGCAAGCCGTCTTCCCAGAGGGCGCACTCACCAAGCGCATTCGAGTAGGCCTACAGGC
TCAACCTATGCATAGTGAATTGGTAAAGAAGATCTTAGGCAACAAAGCCACCTTCAGCCCAATAGTCACT
TTGGAACCCAGGAGAAGAAAATTCCACAAGCCAATTACTATGACTATTCCTGTCCCCAAAGCTTCAAGTG
ATGTCATGCTGAATGGTTTTGGGGGAGACGCACCAACCTTAAGATTACTGTGCAGCATAACAGGTGGAAC
TACGCCTGCCCAGTGGGAAGACATCACAGGAACAACGCCACTAACATTTGTCAATGAGTGTGTTTCCTTT
ACAACCAACGTGTCTGCCAGGTTCTGGCTGATAGACTGTCGACAGATTCAGGAGTCTGTTGCCTTTGCAT
CACAAGTGTATAGAGAAATTATCTGTGTGCCATATATGGCCAAATTTGTAGTGTTTGCCAAGTCACATGA
CCCCATTGAAGCCAGGTTGCGGTGTTTCTGTATGACAGATGACAAAGTAGATAAGACCCTTGAACAACAA
GAAAACTTCTCTGAGGTGGCCAGGAGCAGGGATGTGGAGGTATTGGAAGGAAAACCTATTTATGTTGATT
GTTTTGGCAACCTGGTTCCACTAACCAAGAGTGGCCAACATCATATATTCAGCTTTTTTGCCTTCAAAGA
AAATAGACTTCCTCTCTTTGTCAAGGTTCGTGACACAACCCAGGAACCTTGCGGGCGACTCTCATTTATG
AAGGAACCCAAATCCACAAGAGGACTGGTGCATCAAGCTATTTGCAACTTAAACATCACCCTGCCAATTT
ATGCCAAGGAATCGGAGTCAGATCAAGAGCCGGAGGAAGAGATCGGTATGACATCCGAAAAAAATCCCCA
AGATGAGCAGGAGCGGATGGAAGAAAGGCTGGCTTACATCGCTGATCACCTTGGCTTCAGCTGGACAGAA
TTAGCAAGAGAACTGGATTTCACTGAGGAGCAAATTCACCAAATTCGAATCGAGAACCCCAACTCCCTTC
AAGATCAGAGCCACGCACTGCTCAAGTACTGGCTGGAGAGGGATGGGAAGCATGCCACAGATACCATCCT
CATCGAATGCCTCACCAAGATCAACAGGATGGACATTGTACATCTCCTGGAGACCAACACAGAGCCCCTC
CAGGAGCGCATGGGCCGCAGCTATGCAGAAATAGAGCAGACCATTACGCTGGACCACAGTGAAGGATTTT
CAGTCCTTCCAGACGAGCTCTGTGCTGCCAAGGAGAAGAAGGAGCAGGAAGCTTCCAAAGAAAGCGAGTC
TAGCGACCACCCGCCCATGGTCTCCGAAGAAGACATATCTGTCGGTTATTCCACATTTCAGGATTGCCTC
CCCAAAACTGAAGGGGACAGCCCAGCAGCAGCACTGTCTCCTCAAATGCACCAGGAGCCAGTTCAACAAG
ATTTCTCAGGGAAAACGCAAGACCAGCAGGAATATTATGTAACAACACCAGGGGCAGAAGTGGAAGACCC
TCAGAAGGCCACAGCCGTTCCTGACTCTCTCTGTAAGACTCCTGAGGACATCAGTACCCCTCCTGAGGGA
ACAAAGCCTTGTCTCCAGACCCCGGTGACTAGCGAACGTGGTTCTCCGATTGTGCAAGAACCTGAGGAGG
CTTCCGAGCCCAAAGAGGAGAGTTCTCCAAGGAAAACTAGCCTGGTCATTGTTGAGTCAACAGACGACCA
GTCTCAGGTCTTTGAAAGACTGGATGGTGATGCCGCTTTTCAAAAGGGAGACGATATGCCTGACATACCC
CCAGAGACGGTCACAGAGGAAGAATATGTTGATGAGAATGGACACACCGTGGTGAAGAAGGTTACCCGGA
AAATCATTAGGCGGTACGTTTCCTCTGATGGCACAGAGAAGGAGGAGGTTACCATGCAGGGAATGCCTCA
GGAGCCAGTCAACATTGAGGATGGGGACAATTATTCCAAAGTGATAAAGCGCGTGGTATTGAAGAGTGAC
ACCCAGCAGTCAGAGGTGACTTTGTCTGAACCCAGCGTTTTGTCCAGTACCTCACAGTTTCAGGCCGAGC
CAGTAGAAGGCCGTAGAGTCAGCAAAGTTGTTAAAACAACCATGGTACACGGAGAACGGATGGAGAAGAG
TCTTGGGGACTCTAGCTTAGCCACTGACCTTCCTTCAGCCAAAGATGACTTTGAAGAGGACAACAATGAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211532 representing BC059251
Red=Cloning site Green=Tags(s)

MTEVLDVSDEEGDDTVTGDGGEYLRPEDLKELGDDSLPSSQFLDGMNYLRYSLEGGRSDSLRSFSSDRSH
TLSHASYLRDSAMIDDTVVIPSHQVSALAKEAERNSYRLSWGTENLDNVALSSSPIHSGFLVSFMVDARG
GAMRGCRHNGLRIIIPPRKCTAPTRVTCRLVKRHRLATMPPMVEGEGLASRLIEVGPSGAQFLGPVIVEI
PHFAALRGKERELVVLRSENGDSWKEHFCDYTEDELNEILNGMDEVLDSPEDLEKKRICRIITRDFPQYF
AVVSRIKQDSNLIGPEGGVLSSTVVSQVQAVFPEGALTKRIRVGLQAQPMHSELVKKILGNKATFSPIVT
LEPRRRKFHKPITMTIPVPKASSDVMLNGFGGDAPTLRLLCSITGGTTPAQWEDITGTTPLTFVNECVSF
TTNVSARFWLIDCRQIQESVAFASQVYREIICVPYMAKFVVFAKSHDPIEARLRCFCMTDDKVDKTLEQQ
ENFSEVARSRDVEVLEGKPIYVDCFGNLVPLTKSGQHHIFSFFAFKENRLPLFVKVRDTTQEPCGRLSFM
KEPKSTRGLVHQAICNLNITLPIYAKESESDQEPEEEIGMTSEKNPQDEQERMEERLAYIADHLGFSWTE
LARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTILIECLTKINRMDIVHLLETNTEPL
QERMGRSYAEIEQTITLDHSEGFSVLPDELCAAKEKKEQEASKESESSDHPPMVSEEDISVGYSTFQDCL
PKTEGDSPAAALSPQMHQEPVQQDFSGKTQDQQEYYVTTPGAEVEDPQKATAVPDSLCKTPEDISTPPEG
TKPCLQTPVTSERGSPIVQEPEEASEPKEESSPRKTSLVIVESTDDQSQVFERLDGDAAFQKGDDMPDIP
PETVTEEEYVDENGHTVVKKVTRKIIRRYVSSDGTEKEEVTMQGMPQEPVNIEDGDNYSKVIKRVVLKSD
TQQSEVTLSEPSVLSSTSQFQAEPVEGRRVSKVVKTTMVHGERMEKSLGDSSLATDLPSAKDDFEEDNNE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC059251
ORF Size 3152 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC059251, AAH59251
RefSeq Size 4997 bp
RefSeq ORF 3152 bp
Locus ID 109676
Gene Summary Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions (PubMed:19109891). In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites (PubMed:12571597). Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:15262991). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (PubMed:19007774). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (PubMed:27718357).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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