Smarca1 (NM_053123) Mouse Tagged ORF Clone
CAT#: MG211523
- TrueORF®
Smarca1 (tGFP-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (Smarca1)
ORF Plasmid: DDK
"NM_053123" in other vectors (4)
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CNY 9,220.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 5730494M04Rik; Snf2l |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG211523 representing NM_053123
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGGACACGGCCACAGAGGCAGCCACCGTGGCAGTTTCGGATGCCAGAGCCACAGTCGTGGTTG TAGAGGATGAGCAGCCCGGGCCGTCCACCTTTAAAGAGGAGGGAGCGGCTGCTGCCGCCACCGAAGGCAC CACGGCCACTGAGAAGGGCGAGAAGAAGGAGAAAATCACTTCTCCATTTCAACTCAAACTTGCTGCTAAA GCTTCCAAATCTGAAAAGGAAATGGATCCAGAATATGAAGAGAAAATGGTAAATATGCCCTTGAAAGCAG ACCGCGCAAAGAGATTTGAGTTTTTACTGAAGCAGACGGAACTTTTTGCACATTTCATTCAGCCTTCAGC ACAGAAGTCTCCAACATCTCCTCTCAACATGAAACTGGCTCGTCCTCGAGTAAAGAAAGATGACAAGCAG AGCTTGATTTCCGTTGGAGACTATCGCCACAGGCGTACAGAACAAGAAGAGGATGAGGAGCTTCTCTCAG AGAGTAGGAAAACATCGAATGTGTGTGTCAGATTCGAGGTCTCCCCTTCCTATGTGAAAGGAGGACCGCT GAGAGATTATCAGATCCGAGGACTAAACTGGTTGATTTCTTTGTATGAAAATGGAGTCAATGGTATTTTA GCTGATGAAATGGGTCTTGGGAAGACTTTACAAACCATTGCCTTGCTTGGTTACCTGAAGCACTACAGAA ACATTCCTGGACCCCACATGGTTTTAGTTCCCAAGTCTACTTTACATAACTGGATGAATGAATTTAAACG ATGGGTTCCATCTCTCCGTGTTATTTGTTTCGTTGGAGACAAAGATGTGAGAGCAGCTTTTATTCGTGAT GAAATGATGCCAGGAGAGTGGGATGTTTGCGTGACTTCTTATGAGATGGTAATTAAAGAAAAATCTGTGT TTAAAAAGTTTCACTGGCGATACTTAGTCATCGATGAAGCTCACAGAATAAAAAATGAAAAATCTAAGCT TTCAGAGATTGTTCGTGAGTTCAAGTCAACTAATCGCTTACTTCTAACTGGGACACCTTTGCAGAATAAC TTGCATGAGCTCTGGGCATTACTTAATTTTTTATTGCCGGATGTCTTTAATTCTGCAGATGACTTTGATT CTTGGTTTGACACTAAAAATTGCCTTGGTGATCAAAAGCTTGTGGAAAGACTCCATGCAGTTTTAAAACC ATTTTTGTTACGCCGGATAAAAACCGATGTAGAGAAGAGTCTGCCTCCTAAGAAGGAAATAAAGATTTAC TTGGGCCTGAGTAAAATGCAACGAGAATGGTATACAAAAATTCTGATGAAAGATATTGATGTCTTAAACT CTTCTGGCAAGATGGACAAGATGCGACTCTTGAATATTCTGATGCAGCTCCGAAAGTGTTGTAATCATCC TTATCTGTTTGATGGAGCAGAGCCTGGTCCACCTTATACGACAGATGAGCACATTGTCGGCAATAGTGGT AAAATGGTCGCTCTGGATAAGCTATTGGCGAGAATTAAAGAACAGGGATCAAGAGTTCTCATCTTTAGCC AGATGACTCGACTGTTAGACATTTTGGAGGATTATTGTATGTGGCGTGGTTATGAGTATTCTCGACTGGA TGGACAAACCCCACATGAAGAAAGAGAGGAAGCGATAGATGCCTTCAATGCTCCAAATAGCAGCAAATTC ATCTTTATGCTGAGTACTAGGGCTGGAGGACTCGGAATTAACTTGGCAAGTGCTGATGTGGTCATCCTAT ATGATTCAGACTGGAATCCACAGGTTGATTTACAAGCTATGGATCGGGCACATCGCATTGGTCAGAAGAA GCCAGTACGAGTCTTCCGTCTCATTACAGACAACACAGTGGAAGAGAGGATTGTAGAAAGAGCTGAGATA AAACTGAGACTTGATTCAATTGTTATACAGCAAGGAAGACTCATTGACCAACAATCTAACAAGCTGGCAA AAGAGGAAATGCTACAAATGATCCGTCATGGAGCTACACATGTTTTTGCCTGTAAAGAAAGTGAGCTGAC AGATGAGGACATTGTAACTATTCTGGAAAGAGGAGAAAAGAAGACTGCAGAGATGAATGAACGCATGCAG AAAATGGGAGAGTCATCTCTAAGAAATTTTAGAATGGACCTTGAACAAAGTTTGTACAAGTTTGAAGGAG AAGATTACAGAGAAAAACAAAAGCTGGGTACAGTGGAATGGATTGAACCTCCTAAACGAGAACGCAAAGC CAACTATGCAGTGGATGCCTACTTTAGAGAGGCTTTACGTGTCAGCGAGCCAAAGATCCCAAAGGCTCCT CGGCCTCCAAAACAGCCAAATGTTCAAGATTTTCAATTTTTTCCACCGCGCTTATTTGAGCTCCTGGAAA AGGAAATTCTTTATTATCGGAAGACTATAGGTTACAAGGTCCCAAGAAATCCTGAGATCCCCAACCCAGC TATAGCTCAAAGAGAAGAGCAAAAAAAGATTGATGGAGCGGAACCTCTTACACCACAAGAGACTGAAGAG AAGGATAAACTTCTCACACAAGGTTTCACAAACTGGACTAAGCGAGATTTTAACCAGTTTATTAAAGCTA ATGAGAAGTATGGGAGAGATGACATTGATAATATAGCTCGAGAGGTGGAGGGCAAGTCCCCTGAGGAGGT CATGGAGTATTCAGCTGTATTTTGGGAACGCTGCAATGAATTACAGGACATTGAGAAAATTATGGCTCAA ATTGAACGTGGAGAAGCAAGAATTCAGCGAAGGATCAGTATTAAGAAAGCACTGGATGCCAAAATTGCAA GATACAAGGCTCCATTTCATCAGTTGCGTATTCAGTATGGAACCAGTAAAGGAAAGAACTATACTGAAGA GGAAGACAGATTCTTGATCTGTATGTTACACAAAATGGGTTTTGACAGAGAAAATGTATATGAAGAATTA AGGCAGTGTGTACGGAATGCTCCCCAGTTTAGATTTGACTGGTTCATCAAGTCGAGAACTGCCATGGAAT TTCAGAGACGCTGTAATACTTTGATTTCATTGATTGAAAAAGAAAATATGGAAATTGAGGAAAGAGAGAG AGCAGAAAAGAAGAAACGGGCAACTAAAACTCCAATGGTAAAATTTTCAGCATTTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211523 representing NM_053123
Red=Cloning site Green=Tags(s) MEPDTATEAATVAVSDARATVVVVEDEQPGPSTFKEEGAAAAATEGTTATEKGEKKEKITSPFQLKLAAK ASKSEKEMDPEYEEKMVNMPLKADRAKRFEFLLKQTELFAHFIQPSAQKSPTSPLNMKLARPRVKKDDKQ SLISVGDYRHRRTEQEEDEELLSESRKTSNVCVRFEVSPSYVKGGPLRDYQIRGLNWLISLYENGVNGIL ADEMGLGKTLQTIALLGYLKHYRNIPGPHMVLVPKSTLHNWMNEFKRWVPSLRVICFVGDKDVRAAFIRD EMMPGEWDVCVTSYEMVIKEKSVFKKFHWRYLVIDEAHRIKNEKSKLSEIVREFKSTNRLLLTGTPLQNN LHELWALLNFLLPDVFNSADDFDSWFDTKNCLGDQKLVERLHAVLKPFLLRRIKTDVEKSLPPKKEIKIY LGLSKMQREWYTKILMKDIDVLNSSGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDEHIVGNSG KMVALDKLLARIKEQGSRVLIFSQMTRLLDILEDYCMWRGYEYSRLDGQTPHEEREEAIDAFNAPNSSKF IFMLSTRAGGLGINLASADVVILYDSDWNPQVDLQAMDRAHRIGQKKPVRVFRLITDNTVEERIVERAEI KLRLDSIVIQQGRLIDQQSNKLAKEEMLQMIRHGATHVFACKESELTDEDIVTILERGEKKTAEMNERMQ KMGESSLRNFRMDLEQSLYKFEGEDYREKQKLGTVEWIEPPKRERKANYAVDAYFREALRVSEPKIPKAP RPPKQPNVQDFQFFPPRLFELLEKEILYYRKTIGYKVPRNPEIPNPAIAQREEQKKIDGAEPLTPQETEE KDKLLTQGFTNWTKRDFNQFIKANEKYGRDDIDNIAREVEGKSPEEVMEYSAVFWERCNELQDIEKIMAQ IERGEARIQRRISIKKALDAKIARYKAPFHQLRIQYGTSKGKNYTEEEDRFLICMLHKMGFDRENVYEEL RQCVRNAPQFRFDWFIKSRTAMEFQRRCNTLISLIEKENMEIEERERAEKKKRATKTPMVKFSAFS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053123 |
ORF Size | 3138 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053123.5 |
RefSeq Size | 4022 bp |
RefSeq ORF | 3141 bp |
Locus ID | 93761 |
UniProt ID | Q6PGB8 |
Gene Summary | Energy-transducing component of the NURF (nucleosome-remodeling factor) and CERF (CECR2-containing-remodeling factor) complexes, which facilitate the perturbation of chromatin structure in an ATP-dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202585 | Smarca1 (untagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (Smarca1), (10ug) |
CNY 2,000.00 |
|
MR211523 | Smarca1 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (Smarca1) |
CNY 7,288.00 |
|
MR211523L3 | Lenti ORF clone of Smarca1 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (Smarca1) |
CNY 10,260.00 |
|
MR211523L4 | Lenti ORF clone of Smarca1 (mGFP-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (Smarca1) |
CNY 10,260.00 |
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