Ppfia3 (BC058404) Mouse Tagged ORF Clone

CAT#: MG211515

  • TrueORF®

Ppfia3 (tGFP-tagged) - Mouse protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3

ORF Plasmid: DDK tGFP



  "BC058404" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 11,500.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2410127E16Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211515 representing BC058404
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTGCGAGGTGATGCCTACCATCAGCGAGGATGGCCGGCGGGGCTCGGCGCTGGGCCCGGACGAGG
CCGGCGGCGAGCTGGAGCGCCTCATGGTCACGATGCTCACAGAGCGCGAGCGCCTGCTGGAGACGCTGCG
AGAGGCGCAGGACGGGTTGGCGACAGCGCAGCTACGACTGCGCGAGCTCGGCCACGAGAAGGACTCGCTG
CAGCGGCAGCTCAGCATCGCTCTGCCTCAGGAGTTTGCGGCTCTGACGAAGGAGCTTAACTTATGTCGGG
AACAGCTTCTGGAGAGGGAAGAAGAGATCGCAGAGCTGAAGGCGGAGCGAAACAACACCCGGCTCCTTCT
GGAACACCTTGAGTGCCTGGTGTCCAGGCACGAACGTTCCCTGCGAATGACTGTGGTGAAACGCCAAGCC
CAGTCCCCTGGAGGGGTCTCCTCTGAGGTGGAGGTGCTCAAGGCTCTAAAATCCCTCTTTGAACACCACA
AAGCCCTGGATGAGAAGGTCCGGGAAAGATTGCGGATGGCATTGGAGAATGAGTTGGCCAGCAAAGAGTC
CCTGTACAGACAGAGTGAAGAGAAGAGCCGTCAGCTGGCAGAGTGGCTGGATGACGCCAAGCAGAAGCTG
CAACAGACCCTGCAGAAGGCCGAGACCTTACCAGAGATAGAGGCTCAGCTGGCGCAGCGCGTGGCTGCTC
TGAACAAGGCTGAGGAACGCCATGGGAACTTTGAAGAGAGGCTTCGGCAGCTGGAGGCACAGCTGGAGGA
GAAGAACCAGGAGCTGCAGCGGGCCCGGCAGAGGGAGAAAATGAACGATGACCACAATAAGCGACTGTCA
GAGACGGTGGACAAGCTGCTAAGTGAATCCAACGAACGGTTACAGCTTCACCTCAAAGAACGTATGGGGG
CACTAGAGGAGAAGAACTCACTGAGTGAGGAGATTGCTAACATGAAGAAACTTCAAGATGAGCTGCTGCT
GAATAAGGAGCAGCTCTTGGCTGAGATGGAACGGATGCAGATGGAGATCGACCAGCTCCGGGGAAGGCCG
CCCTCCTCCTATTCCAGGTCCCTTCCTGGCAGTGCCCTGGAGCTCCGTTACTCCCAGGCACCAACCTTAC
CTTCTGGTGCCCCTCTGGATCCTTATGGGGCTGGCAGTGGCCGGGCAGGCAAGAGGGGCCGCTGGTCGGG
GGCCAAGGATGAGTCTTCCAAGGACTGGGATCGGTCTGCCCCTGCTGGGTCCATCCCACCCCCGTTCCCT
GGAGAGCTGGATGGCTCAGATGAAGAGGAGGCCGAGGGGATGTTCGGGGCTGAGCTGCTGTCCCCCAGTG
GGCAAGCTGATGTGCAGACGCTCGCTATCATGCTCCAGGAGCAATTGGAAGCCATCAACAAGGAAATCAA
GCTAATTCAAGAAGAGAAAGAGACGACAGAGCAGCGTGCAGAAGAGCTGGAGAGCCGGGTGTCCAGTTCT
GGCCTTGACTCGCTGGGCCGGTACCGAAGCAGCTGCTCACTGCCACCCTCCCTCACCACCTCCACCCTCG
CCAGCCCCTCACCTCCCAGCTCTGGCCATTCCACCCCTCGTCTGGCACCCCCCAGTCCTGCCCGGGAGGG
TACTGACAAGACCAACCATGTCTCTAAGGAGGAAGCTGGAGTCCCACGAGGGGAGGGTCCAGCCGTTCCA
GGAGACACCCCACCACCCACGCCCCGCTCTGCCCGTCTTGAGAGGATGGCCCAGGCTTTGGCGCTGCAAG
CAGGGTCACCGGAAGATGGAGCACCTCCCCGGGGAAGTGAGAGCACCCCAGATTCCCTTCACAAAGCCCC
CAAGAGGAAGAGCATCAAGTCGTCCATAGGCCGGCTCTTCGGCAAGAAAGAGAAGGGCCGGATGGGACCC
CCAGGCCGGGAGAGTGTTTCTCTGGCCGGGACACCCTCAGATGAGACACTGGCCACTGACCCTCTGGGGC
TAGCCAAGCTGACTGGTCCAGGAGATAAGGACAGGAGGAACAAGAGGAAGCACGAACTTCTGGAAGAGGC
CTGTCGCCAGGGCCTGCCTTTTGCTGCCTGGGATGGACCCACCGTGGTCTCCTGGCTGGAGCTCTGGGTG
GGCATGCCGGCGTGGTACGTGGCCGCCTGCCGGGCCAATGTCAAGAGCGGCGCCATCATGGCCAACCTGT
CAGACACGGAGATCCAGAGGGAGATCGGCATCAGCAATCCACTGCACAGGCTCAAGCTACGCCTCGCCAT
TCAGGAGATGGTGTCGCTCACATCACCCTCGGCTCCTGCCTCCTCACGCACTCCCACAGGAAACGTGTGG
ATGACACACGAGGAGATGGAGTCTCTGACGGCAGCTACAAAACCCGAGACCAAGGAGATCAGCTGGGAGC
AGATCCTGGCATATGGCGACATGAACCACGAGTGGGTGGGGAACGACTGGCTGCCCAGCCTTGGGCTGCC
CCAGTACCGAAGCTACTTCATGGAGTCGCTAGTTGATGCGCGGATGTTGGACCATTTGAACAAGAAGGAA
CTGCGAGGCCAACTCAAGATGGTGGACAGCTTCCACAGGGTTAGTCTGCACTATGGTATTATGTGCCTGA
AGCGGCTCAACTATGACCGGAAGGACCTGGAGCGGAGGAGGGAAGAAAGCCAGACCCAAATCAGAGACGT
GATGGTGTGGTCCAACGAACGGGTCATGGGTTGGGTGTCTGGTCTTGGCCTGAAGGAATTTGCTACGAAT
CTCACGGAGAGTGGGGTGCACGGCGCACTGTTGGCTCTGGATGAAACCTTCGACTACTCCGATCTGGCCT
TGCTTCTACAGATACCTACGCAGAATGCACAAGCTCGGCAACTGCTGGAGAAAGAATTCAGTAACCTCAT
CTCCTTAGGCACAGACAGACGGCTGGATGAGGACAGTGCCAAGTCCTTCAGCCGCTCCCCATCCTGGAGG
AAGATGTTCCGCGAGAAGGACCTCCGGGGTGTAACCCCCGATTCAGCCGAGATGTTACCCCCCAACTTTC
GCTCAGCTGCCGCAGGAGCCCTAGGCTCTCCCGGGCTTCCTCTCCGCAAACTGCAGCCTGAAGGCCAGAC
TTCTGGGAGTTCGCGAGCAGATGGTGTTTCGGTCCGGACTTACTCCTGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211515 representing BC058404
Red=Cloning site Green=Tags(s)

MMCEVMPTISEDGRRGSALGPDEAGGELERLMVTMLTERERLLETLREAQDGLATAQLRLRELGHEKDSL
QRQLSIALPQEFAALTKELNLCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQA
QSPGGVSSEVEVLKALKSLFEHHKALDEKVRERLRMALENELASKESLYRQSEEKSRQLAEWLDDAKQKL
QQTLQKAETLPEIEAQLAQRVAALNKAEERHGNFEERLRQLEAQLEEKNQELQRARQREKMNDDHNKRLS
ETVDKLLSESNERLQLHLKERMGALEEKNSLSEEIANMKKLQDELLLNKEQLLAEMERMQMEIDQLRGRP
PSSYSRSLPGSALELRYSQAPTLPSGAPLDPYGAGSGRAGKRGRWSGAKDESSKDWDRSAPAGSIPPPFP
GELDGSDEEEAEGMFGAELLSPSGQADVQTLAIMLQEQLEAINKEIKLIQEEKETTEQRAEELESRVSSS
GLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKTNHVSKEEAGVPRGEGPAVP
GDTPPPTPRSARLERMAQALALQAGSPEDGAPPRGSESTPDSLHKAPKRKSIKSSIGRLFGKKEKGRMGP
PGRESVSLAGTPSDETLATDPLGLAKLTGPGDKDRRNKRKHELLEEACRQGLPFAAWDGPTVVSWLELWV
GMPAWYVAACRANVKSGAIMANLSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPASSRTPTGNVW
MTHEEMESLTAATKPETKEISWEQILAYGDMNHEWVGNDWLPSLGLPQYRSYFMESLVDARMLDHLNKKE
LRGQLKMVDSFHRVSLHYGIMCLKRLNYDRKDLERRREESQTQIRDVMVWSNERVMGWVSGLGLKEFATN
LTESGVHGALLALDETFDYSDLALLLQIPTQNAQARQLLEKEFSNLISLGTDRRLDEDSAKSFSRSPSWR
KMFREKDLRGVTPDSAEMLPPNFRSAAAGALGSPGLPLRKLQPEGQTSGSSRADGVSVRTYSC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC058404
ORF Size 3129 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC058404.1
RefSeq Size 4191 bp
RefSeq ORF 3131 bp
Locus ID 76787
Gene Summary May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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