Krba1 (BC066816) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	A930040G15Rik; AI448780
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211514 representing BC066816 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGAAAACTACGAGACGTTGGTGTCTGTGGGGACCTCTGAGCTGCTTCCTCTCTCTGCTTTCCTGT CACCTGCAGAGGCTGGAGGAGCCACATCAGGAGAGAGCCACCAGGATAAGGGACAAAAACCCCATTTGGA GCATAGTTCCCAGGGAGAGCAGCCTCAGCAGAGCCTACACCTCACCGCATTAGTGCAGCTGGTGAAGGAG ATTCCAGAGTTCTTGTTTGGAGAAGTGAAGGGTACTGAGGACTACTCCGAGAGTGGGAGCACCAGTCTGG ATGGGGAGCAAACAAGCCCCGAGGTAGCTGTGGTTGTGGAAGCTTGCCCTCCCCGAGGCCTGCTCAGTTC TCTTCCGGAGAGCCCTGCAAGCCACCCCAGCCTGGCCACCACACCCACAGGCAGCTCAACTTCCGGTGGC CCTCCTGGAGACTGGGCACACGGAAGCCCCTTACCTGCTATAGGAACTGATGATAAGCCATTATCTATAG AGAAGGAAGGTGTAGGAGCCTCGAGAGAGACCTCCATTCATTCCACTCAAAGCCTGGGCCAGAGCAAGAG TTACCTAAGACAGGAGCGAGGCAGCATGGGAACAGGAACCCTTCCTGAGAACAGCCCATTGCAAGGCCTC ATCAACTGTTTGAAGGAGATCCTTGTGCCCAGACCCCAGCACCGGGGGACAGCCCCAGACTTGCCGCCTT CTCTCCCTGGCTTGAGTGTGTTGAAGCAGACGAGAGCTGAGGTAGAGGCTGGGAGCCTGCCCTGCCCGGT GAAGACAGAGGCAGCATCCGGAGATTGTCCCCTTCAGGGCCTGCTGAACTGTCTGAAGGAGATCCCAAAA GCTCCAGACCGACGTCCCAGCCCCTCAGGAGCATCAGACTTGCAGCTGCAGGAGGACCCAGGGAAAAGAC ATTCTGGAGGGATGAGACACCTCCAGACTCCTCCCCACCCTAGTCATGAAGCTGGCAGTATGCTTGCCAC GGTGAAGGTAGAAGATGGCTGGGCCCAGAGTCCCCCAGTGCCAGCATCCTGCCAGCTTAGCAGGCAAGGC TATAGCTCCTATTCCACTGGAGACAACAGAGAGGTCCGTGTGCCCCGCTGGGGCCCCATGACTCTAGCCA GCAGGGCCTCAAGCTCACCCCTAGAAGCTCTGGAGGCCTGTCCGAAGGGCATCCCTCCAGGTGGGTCATC ACCTCTTCAGTCACTAGCCATCTCATGGTCCAGAAGTCCTCAGCTAGGAGATGCCGGGTCTCAGAGGTTT GAGCTACAGCAACAAGGATCTCACAGTGAAGAAGCTACAAGGGAGCCACTTCTGCCTCTGAGCTTGCAGG GCTACATGAGAGAAGGACCTGGGGTTCAACCCTGTGGCTCCCAGGGTACCCCTACCAGCTTCTCCTCAGC CAGCAGCAGTGATGGGGATCTGGATTTCAGGAGCCCCAGGAGCAGCCAGGGGCAACGGCTTGGGAAAGGC TATCTACCAGGAAACTCTCCACTCCAAGGCCTGGAGAACTGCCTGAGAGAGATCCCTATTCCCAGGCCAC AGGCTGCCTGGCCATGCTCCTCAGCTGTCAACAGGGGATTGAAGAGAACAGAGCCCAGGAACTGGACTGG AGACAGAGAAGGACTGAGAGGTGAGGCCTCTGAGCCACCCCACCTCAGACAGCGTCCTGGAGAAGTGCCC AGCAGGAGTCTGCATCAAGACAGTCCACAGACCTGTACTTCCACCTGCCACCAAGTGACCACCAGGCCAG GAACATGGCAATGGCCACAAGAGGAGACAGCCACCATGCCCTCCCCTCTGCACCGCCTGGAGAACTCTCT GAGGGGGATCTTGCCTGTGAGGCCCTTGCGTTTCACCTGCGTGACTGGCCCTGGCCCCAGTCCCAGCCCT TGCTCCAGCTCCAGCTTCAGCAGCTCTGATGGAGAAGACCTAAGACCAGAGCCTGCATTTTGGCAGTCAC CCCTCCAGCAGAAAGACCAGCCTCCCTCCTGTAAGGACCCTGTTCGTCTGTGCCCTGTCTCTGGCGCATC TCCAAGAGTCAACAGCAATAGCTGTTCTGCGGAAGACCGTGAGAGAACAGAGCCCAGGGACTGCAGCAGC CTCAGCGCAGGAAGAGCAGAAGAGAAGCCCCACCCACCCAGAAGAGAAGATGGTGCAGAGCGCACACGCC AGCCTGGGCCTGTCACCAATGCTGAAGGAAAAGGAGCAGCAGCTGGCCACCCATCGCCTGCCCCTCAGCT GGAGGAAAAGCCTGAGCCCAAGGGTACTGAAGACTCCAGGGACCTGGAGCCTGGACATAGACCACCCAGT GCCGCAGCCAGGACCCAAGGGAAGCTGCTCTCTGGAGACCCTCCGGAGTCACCTAGCAAGTCTCCCCTTC CCACAACTGTCTTGTCAAAATGGTCACCCACTTCTCTTCAGCCACCATGCCCCTGTGGCAGGTCCTTGCA GCAGGAGCTGCATAACCTTGGTACTGCCCTCACGGACAAGCTAGACCGGCTTGCAGCAGCCTTGGCAGGC CTGACTCAGGAAGTTGCAACCATGAAGACCCAAATGGATCAACTGCGAAGGCACCCACGGAGCCTTGGCC CAAAAGGTCAGGGTTCCTGGCAGTTGGCCCTCCCCCAGAGACCTCGCTGGGTCAACAGACTGGGCCACAG ACATCTACCCTACTGGAGACAGAAGGGCCCCACCAGGCCCAGACCAAAGATTCTGCGGACCCAGGCAGAA GGCTGCAAGACTAGTGACCGCCCAGGACTCTCTAGAGGGAAGGGCAGTTTGGTGCCTCAGCTGCCTCCAG AGGCTTCCTTGGTAGAATCTTCCAGGCCCACCTGTAGTTCATCCCAGCAGATCTCCTCTACGCCTGGAGG CCACACTGTGCTGACTGCACACCCTCCTCTGGAGCACACTGCATGCCACCAGAATCCCCTCTCCCCTTCA GTGCCTACTTCAGTGCAGGTCCCCCTTGTGGCCTCACCTGCAACCAGTGCAGACACAGAACCTCCGGCTG CTAGAGTGGCAGCCATCAGCATTCCAAACCAGCCCAAGGAACCTGACAGCCTGCTAGGGGAAGCCCTCAG CAGAGACCTCTGGGGAGGTGACCACCGGGACCCAAGGTGGGGGGCCCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211514 representing BC066816 Red=Cloning site Green=Tags(s) MRENYETLVSVGTSELLPLSAFLSPAEAGGATSGESHQDKGQKPHLEHSSQGEQPQQSLHLTALVQLVKE IPEFLFGEVKGTEDYSESGSTSLDGEQTSPEVAVVVEACPPRGLLSSLPESPASHPSLATTPTGSSTSGG PPGDWAHGSPLPAIGTDDKPLSIEKEGVGASRETSIHSTQSLGQSKSYLRQERGSMGTGTLPENSPLQGL INCLKEILVPRPQHRGTAPDLPPSLPGLSVLKQTRAEVEAGSLPCPVKTEAASGDCPLQGLLNCLKEIPK APDRRPSPSGASDLQLQEDPGKRHSGGMRHLQTPPHPSHEAGSMLATVKVEDGWAQSPPVPASCQLSRQG YSSYSTGDNREVRVPRWGPMTLASRASSSPLEALEACPKGIPPGGSSPLQSLAISWSRSPQLGDAGSQRF ELQQQGSHSEEATREPLLPLSLQGYMREGPGVQPCGSQGTPTSFSSASSSDGDLDFRSPRSSQGQRLGKG YLPGNSPLQGLENCLREIPIPRPQAAWPCSSAVNRGLKRTEPRNWTGDREGLRGEASEPPHLRQRPGEVP SRSLHQDSPQTCTSTCHQVTTRPGTWQWPQEETATMPSPLHRLENSLRGILPVRPLRFTCVTGPGPSPSP CSSSSFSSSDGEDLRPEPAFWQSPLQQKDQPPSCKDPVRLCPVSGASPRVNSNSCSAEDRERTEPRDCSS LSAGRAEEKPHPPRREDGAERTRQPGPVTNAEGKGAAAGHPSPAPQLEEKPEPKGTEDSRDLEPGHRPPS AAARTQGKLLSGDPPESPSKSPLPTTVLSKWSPTSLQPPCPCGRSLQQELHNLGTALTDKLDRLAAALAG LTQEVATMKTQMDQLRRHPRSLGPKGQGSWQLALPQRPRWVNRLGHRHLPYWRQKGPTRPRPKILRTQAE GCKTSDRPGLSRGKGSLVPQLPPEASLVESSRPTCSSSQQISSTPGGHTVLTAHPPLEHTACHQNPLSPS VPTSVQVPLVASPATSADTEPPAARVAAISIPNQPKEPDSLLGEALSRDLWGGDHRDPRWGAH TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC066816
ORF Size	3131 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC066816, AAH66816
RefSeq Size	3986 bp
RefSeq ORF	3131 bp
Locus ID	77827

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