Cntn2 (BC066106) Mouse Tagged ORF Clone

CAT#: MG211507

  • TrueORF®

Cntn2 (tGFP-tagged) - Mouse contactin 2 (cDNA clone MGC:90117 IMAGE:30362931)



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Mouse Protein / Lysate Request

CNY 8,848.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms D130012K04Rik; TAG-1; Tax
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211507 representing BC066106
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGCACCGGCCAGGAAAAGGGCAAGCTTGCTGCTGCTGCTGCTGGCCACAATGGCTCTGGTCTCCT
CTCCAGGATGGAGTTTTTCCCAGGGAACCCCAGCTACCTTTGGACCCGTCTTTGAAGAGCAACCTGTTGG
CCTGCTATTCCCAGAGGAGTCTGCAGAGGATCAGGTGACACTGGCGTGCCGTGCCCGAGCTAGCCCTCCA
GCCACCTATAGGTGGAAGATGAACGGCACAGAGATGAACCTGGAACCTGGTTCCCGTCACCAGCTGATGG
GGGGCAACCTGGTCATCATGAGTCCCACCAAAGCACAGGATGCTGGTGTCTACCAGTGCCTAGCCTCTAA
CCCAGTAGGCACTGTCGTCAGCAAGGAAGCTGTCCTCCGCTTCGGCTTTCTACAGGAATTCTCCAAGGAG
GAGAGAGACCCCGTGAAAACCCACGAGGGCTGGGGGGTGATGCTGCCCTGTAACCCACCTGCCCACTACC
CAGGTTTGTCCTACCGCTGGCTCCTCAACGAGTTCCCCAACTTCATCCCAACGGATGGGCGTCACTTCGT
GTCCCAGACCACAGGGAACCTGTACATCGCCCGCACCAATGCCTCGGACCTGGGCAACTACTCTTGCCTG
GCTACCAGCCACCTGGACTTCTCCACCAAGAGCGTCTTCAGCAAATTCGCGCAGCTCAACCTGGCTGCTG
AAGATCCTCGACTCTTTGCTCCCAGTATCAAAGCCCGGTTCCCCCCAGAGACGTACGCACTGGTTGGGCA
GCAGGTCACCCTGGAGTGCTTTGCCTTTGGGAACCCTGTTCCCAGGATCAAGTGGCGCAAAGTGGATGGT
TCCTTGTCCCCTCAGTGGGGCACAGCAGAGCCCACCCTGCAGATCCCCAGTGTTAGCTTTGAAGATGAGG
GTACCTATGAATGTGAGGCAGAGAACTCCAAGGGCCGTGACACCGTCCAGGGACGCATCATTGTGCAAGC
TCAGCCTGAGTGGCTAAAGGTGATCTCAGACACAGAAGCCGACATTGGTTCCAACTTACGTTGGGGCTGT
GCTGCGGCAGGCAAGCCTCGGCCCATGGTGCGCTGGCTGAGAAACGGGGAACCTCTGGCCTCCCAGAACC
GGGTGGAGGTCTTGGCTGGGGACCTGCGATTCTCTAAGCTGAACCTGGAGGACTCCGGCATGTACCAGTG
TGTGGCAGAAAACAAGCATGGCACCATCTATGCCAGTGCTGAGCTGGCTGTACAAGCTCTGGCCCCAGAC
TTCAGGCAGAACCCTGTGAGGCGGCTGATCCCTGCAGCCCGAGGGGGAGAGATCAGTATCCCGTGCCAGC
CCCGTGCGGCTCCAAAAGCTACAATACTGTGGAGCAAGGGTACTGAGATTCTGGGGAACAGTACCAGAGT
GACTGTCACTTTGGACGGCACCTTGATTATCAGAAACATCAGTCGATCGGATGAAGGCAAATATACCTGC
TTTGCTGAGAACTTCATGGGCAAAGCCAACAGTACCGGGATCCTGTCTGTGCGAGATGCAACGAAGATCA
CGCTAGCTCCTTCGAGTGCTGACATCAATGTGGGTGATAACCTGACCCTACAGTGCCACGCCTCGCATGA
CCCCACTATGGACCTCACGTTCACCTGGACCCTGGATGACTTCCCTGTTGACTTTGATAAGCCTGGAGGT
CACTACCGGAGAGCCAGTGTGAAGGAGACCATTGGGGACCTGACTATCCTGAATGCCCAGCTACGCCACG
GAGGGACATACACATGCATGGCCCAGACAGTGGTGGATGGTGCATCCAAGGAGGCCACAGTCCTGGTCCG
AGGTCCCCCAGGTCCTCCTGGGGGTGTGGTGGTGAGAGACATTGGAGACACTACTGTTCAGCTTAGCTGG
AGTCGTGGCTTTGACAATCACAGCCCCATTGCCAAGTACACCCTGCAAGCGCGGACTCCACCCTCAGGGA
AATGGAAGCAGGTTCGGACCAATCCTGTGAATATCGAGGGCAACGCGGAAACTGCCCAGGTGCTGGGGCT
CATGCCTTGGATGGACTATGAGTTTCGGGTTTCAGCTAGCAACATCTTGGGCACTGGGGAGCCCAGCGGC
CCCTCCAGCAGAATCCGCACTAAGGAAGCAGTCCCCTCTGTGGCACCATCTGGACTCAGTGGAGGGGGAG
GAGCCCCTGGAGAGCTTACCATCAACTGGACTCCCATGTCACGAGAGTACCAGAATGGAGACGGCTTCGG
CTACCTGCTGTCCTTCCGCAGGCAAGGCAGCTCCAGTTGGCAGACGGCCCGGGTGCCCGGCGCTGATACC
CAGTACTTCGTCTACAGCAACGATAGCATCCATCCCTACACACCCTTTGAGGTCAAGATCCGAAGCTACA
ATCGCCGGGGGGATGGGCCCGAGAGCCTCACTGCCATCGTGTACTCAGCAGAGGAAGAGCCCAAGGTGGC
CCCTGCCAAGGTCTGGGCCAAGGGTTCCTCATCCTCAGAGATGAACGTAAGCTGGGAGCCTGTGCTACAA
GACATGAACGGCATCCTCCTGGGATATGAGATTCGCTACTGGAAAGCCGGGGACAAAGAAGCTGCCGCTG
ACCGAGTGAGGACGGCAGGGCTAGACTCCAGTGCCCGAGTCACCGGCCTGTACCCCAACACCAAGTACCA
TGTAACTGTGAGGGCCTACAACAGGGCTGGCACCGGACCAGCTAGCCCTTCAGCTGATGCCATGACCATG
AAGCCCCCACCACGGCGACCTCCTGGCAACATCTCCTGGACTTTCTCAAGTTCCAGCCTCAGCCTTAAGT
GGGACCCTGTGGTTCCTCTCCGAAATGAATCTACAGTCACTGGCTACAAGATGTTGTATCAGAATGACCT
GCAGCCAACTCCTATGCTCCACCTCACCAGCAAGAACTGGATAGAAATACCAGTACCTGAAGACATTGGT
CACGCTCTGGTGCAGATTCGAACCACGGGGCCTGGAGGGGATGGGATACCAGCCGAAGTCCACATTGTGA
GAAATGGAGGCACCAGCATGATGGTGGAGAGCTCAGCCGTCCGCCCTGCCCATCCTGGCCCGGTGTTCTC
CTGCATGGTTATACTGATGCTCGCCGGATGCCAGAGGCTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211507 representing BC066106
Red=Cloning site Green=Tags(s)

MGAPARKRASLLLLLLATMALVSSPGWSFSQGTPATFGPVFEEQPVGLLFPEESAEDQVTLACRARASPP
ATYRWKMNGTEMNLEPGSRHQLMGGNLVIMSPTKAQDAGVYQCLASNPVGTVVSKEAVLRFGFLQEFSKE
ERDPVKTHEGWGVMLPCNPPAHYPGLSYRWLLNEFPNFIPTDGRHFVSQTTGNLYIARTNASDLGNYSCL
ATSHLDFSTKSVFSKFAQLNLAAEDPRLFAPSIKARFPPETYALVGQQVTLECFAFGNPVPRIKWRKVDG
SLSPQWGTAEPTLQIPSVSFEDEGTYECEAENSKGRDTVQGRIIVQAQPEWLKVISDTEADIGSNLRWGC
AAAGKPRPMVRWLRNGEPLASQNRVEVLAGDLRFSKLNLEDSGMYQCVAENKHGTIYASAELAVQALAPD
FRQNPVRRLIPAARGGEISIPCQPRAAPKATILWSKGTEILGNSTRVTVTLDGTLIIRNISRSDEGKYTC
FAENFMGKANSTGILSVRDATKITLAPSSADINVGDNLTLQCHASHDPTMDLTFTWTLDDFPVDFDKPGG
HYRRASVKETIGDLTILNAQLRHGGTYTCMAQTVVDGASKEATVLVRGPPGPPGGVVVRDIGDTTVQLSW
SRGFDNHSPIAKYTLQARTPPSGKWKQVRTNPVNIEGNAETAQVLGLMPWMDYEFRVSASNILGTGEPSG
PSSRIRTKEAVPSVAPSGLSGGGGAPGELTINWTPMSREYQNGDGFGYLLSFRRQGSSSWQTARVPGADT
QYFVYSNDSIHPYTPFEVKIRSYNRRGDGPESLTAIVYSAEEEPKVAPAKVWAKGSSSSEMNVSWEPVLQ
DMNGILLGYEIRYWKAGDKEAAADRVRTAGLDSSARVTGLYPNTKYHVTVRAYNRAGTGPASPSADAMTM
KPPPRRPPGNISWTFSSSSLSLKWDPVVPLRNESTVTGYKMLYQNDLQPTPMLHLTSKNWIEIPVPEDIG
HALVQIRTTGPGGDGIPAEVHIVRNGGTSMMVESSAVRPAHPGPVFSCMVILMLAGCQRL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC066106
ORF Size 3120 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC066106, AAH66106
RefSeq Size 5868 bp
RefSeq ORF 3122 bp
Locus ID 21367
Gene Summary This gene encodes a member of the contactin family of proteins, part of the immunoglobulin superfamily of cell adhesion molecules. The encoded glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein plays a role in the proliferation, migration, and axon guidance of neurons of the developing cerebellum. Mice lacking a functional copy of this gene exhibit epileptic seizures and elevated expression of A1 adenosine receptors. [provided by RefSeq, Sep 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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