Cntn2 (BC066106) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	D130012K04Rik; TAG-1; Tax
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211507 representing BC066106 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCACCGGCCAGGAAAAGGGCAAGCTTGCTGCTGCTGCTGCTGGCCACAATGGCTCTGGTCTCCT CTCCAGGATGGAGTTTTTCCCAGGGAACCCCAGCTACCTTTGGACCCGTCTTTGAAGAGCAACCTGTTGG CCTGCTATTCCCAGAGGAGTCTGCAGAGGATCAGGTGACACTGGCGTGCCGTGCCCGAGCTAGCCCTCCA GCCACCTATAGGTGGAAGATGAACGGCACAGAGATGAACCTGGAACCTGGTTCCCGTCACCAGCTGATGG GGGGCAACCTGGTCATCATGAGTCCCACCAAAGCACAGGATGCTGGTGTCTACCAGTGCCTAGCCTCTAA CCCAGTAGGCACTGTCGTCAGCAAGGAAGCTGTCCTCCGCTTCGGCTTTCTACAGGAATTCTCCAAGGAG GAGAGAGACCCCGTGAAAACCCACGAGGGCTGGGGGGTGATGCTGCCCTGTAACCCACCTGCCCACTACC CAGGTTTGTCCTACCGCTGGCTCCTCAACGAGTTCCCCAACTTCATCCCAACGGATGGGCGTCACTTCGT GTCCCAGACCACAGGGAACCTGTACATCGCCCGCACCAATGCCTCGGACCTGGGCAACTACTCTTGCCTG GCTACCAGCCACCTGGACTTCTCCACCAAGAGCGTCTTCAGCAAATTCGCGCAGCTCAACCTGGCTGCTG AAGATCCTCGACTCTTTGCTCCCAGTATCAAAGCCCGGTTCCCCCCAGAGACGTACGCACTGGTTGGGCA GCAGGTCACCCTGGAGTGCTTTGCCTTTGGGAACCCTGTTCCCAGGATCAAGTGGCGCAAAGTGGATGGT TCCTTGTCCCCTCAGTGGGGCACAGCAGAGCCCACCCTGCAGATCCCCAGTGTTAGCTTTGAAGATGAGG GTACCTATGAATGTGAGGCAGAGAACTCCAAGGGCCGTGACACCGTCCAGGGACGCATCATTGTGCAAGC TCAGCCTGAGTGGCTAAAGGTGATCTCAGACACAGAAGCCGACATTGGTTCCAACTTACGTTGGGGCTGT GCTGCGGCAGGCAAGCCTCGGCCCATGGTGCGCTGGCTGAGAAACGGGGAACCTCTGGCCTCCCAGAACC GGGTGGAGGTCTTGGCTGGGGACCTGCGATTCTCTAAGCTGAACCTGGAGGACTCCGGCATGTACCAGTG TGTGGCAGAAAACAAGCATGGCACCATCTATGCCAGTGCTGAGCTGGCTGTACAAGCTCTGGCCCCAGAC TTCAGGCAGAACCCTGTGAGGCGGCTGATCCCTGCAGCCCGAGGGGGAGAGATCAGTATCCCGTGCCAGC CCCGTGCGGCTCCAAAAGCTACAATACTGTGGAGCAAGGGTACTGAGATTCTGGGGAACAGTACCAGAGT GACTGTCACTTTGGACGGCACCTTGATTATCAGAAACATCAGTCGATCGGATGAAGGCAAATATACCTGC TTTGCTGAGAACTTCATGGGCAAAGCCAACAGTACCGGGATCCTGTCTGTGCGAGATGCAACGAAGATCA CGCTAGCTCCTTCGAGTGCTGACATCAATGTGGGTGATAACCTGACCCTACAGTGCCACGCCTCGCATGA CCCCACTATGGACCTCACGTTCACCTGGACCCTGGATGACTTCCCTGTTGACTTTGATAAGCCTGGAGGT CACTACCGGAGAGCCAGTGTGAAGGAGACCATTGGGGACCTGACTATCCTGAATGCCCAGCTACGCCACG GAGGGACATACACATGCATGGCCCAGACAGTGGTGGATGGTGCATCCAAGGAGGCCACAGTCCTGGTCCG AGGTCCCCCAGGTCCTCCTGGGGGTGTGGTGGTGAGAGACATTGGAGACACTACTGTTCAGCTTAGCTGG AGTCGTGGCTTTGACAATCACAGCCCCATTGCCAAGTACACCCTGCAAGCGCGGACTCCACCCTCAGGGA AATGGAAGCAGGTTCGGACCAATCCTGTGAATATCGAGGGCAACGCGGAAACTGCCCAGGTGCTGGGGCT CATGCCTTGGATGGACTATGAGTTTCGGGTTTCAGCTAGCAACATCTTGGGCACTGGGGAGCCCAGCGGC CCCTCCAGCAGAATCCGCACTAAGGAAGCAGTCCCCTCTGTGGCACCATCTGGACTCAGTGGAGGGGGAG GAGCCCCTGGAGAGCTTACCATCAACTGGACTCCCATGTCACGAGAGTACCAGAATGGAGACGGCTTCGG CTACCTGCTGTCCTTCCGCAGGCAAGGCAGCTCCAGTTGGCAGACGGCCCGGGTGCCCGGCGCTGATACC CAGTACTTCGTCTACAGCAACGATAGCATCCATCCCTACACACCCTTTGAGGTCAAGATCCGAAGCTACA ATCGCCGGGGGGATGGGCCCGAGAGCCTCACTGCCATCGTGTACTCAGCAGAGGAAGAGCCCAAGGTGGC CCCTGCCAAGGTCTGGGCCAAGGGTTCCTCATCCTCAGAGATGAACGTAAGCTGGGAGCCTGTGCTACAA GACATGAACGGCATCCTCCTGGGATATGAGATTCGCTACTGGAAAGCCGGGGACAAAGAAGCTGCCGCTG ACCGAGTGAGGACGGCAGGGCTAGACTCCAGTGCCCGAGTCACCGGCCTGTACCCCAACACCAAGTACCA TGTAACTGTGAGGGCCTACAACAGGGCTGGCACCGGACCAGCTAGCCCTTCAGCTGATGCCATGACCATG AAGCCCCCACCACGGCGACCTCCTGGCAACATCTCCTGGACTTTCTCAAGTTCCAGCCTCAGCCTTAAGT GGGACCCTGTGGTTCCTCTCCGAAATGAATCTACAGTCACTGGCTACAAGATGTTGTATCAGAATGACCT GCAGCCAACTCCTATGCTCCACCTCACCAGCAAGAACTGGATAGAAATACCAGTACCTGAAGACATTGGT CACGCTCTGGTGCAGATTCGAACCACGGGGCCTGGAGGGGATGGGATACCAGCCGAAGTCCACATTGTGA GAAATGGAGGCACCAGCATGATGGTGGAGAGCTCAGCCGTCCGCCCTGCCCATCCTGGCCCGGTGTTCTC CTGCATGGTTATACTGATGCTCGCCGGATGCCAGAGGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211507 representing BC066106 Red=Cloning site Green=Tags(s) MGAPARKRASLLLLLLATMALVSSPGWSFSQGTPATFGPVFEEQPVGLLFPEESAEDQVTLACRARASPP ATYRWKMNGTEMNLEPGSRHQLMGGNLVIMSPTKAQDAGVYQCLASNPVGTVVSKEAVLRFGFLQEFSKE ERDPVKTHEGWGVMLPCNPPAHYPGLSYRWLLNEFPNFIPTDGRHFVSQTTGNLYIARTNASDLGNYSCL ATSHLDFSTKSVFSKFAQLNLAAEDPRLFAPSIKARFPPETYALVGQQVTLECFAFGNPVPRIKWRKVDG SLSPQWGTAEPTLQIPSVSFEDEGTYECEAENSKGRDTVQGRIIVQAQPEWLKVISDTEADIGSNLRWGC AAAGKPRPMVRWLRNGEPLASQNRVEVLAGDLRFSKLNLEDSGMYQCVAENKHGTIYASAELAVQALAPD FRQNPVRRLIPAARGGEISIPCQPRAAPKATILWSKGTEILGNSTRVTVTLDGTLIIRNISRSDEGKYTC FAENFMGKANSTGILSVRDATKITLAPSSADINVGDNLTLQCHASHDPTMDLTFTWTLDDFPVDFDKPGG HYRRASVKETIGDLTILNAQLRHGGTYTCMAQTVVDGASKEATVLVRGPPGPPGGVVVRDIGDTTVQLSW SRGFDNHSPIAKYTLQARTPPSGKWKQVRTNPVNIEGNAETAQVLGLMPWMDYEFRVSASNILGTGEPSG PSSRIRTKEAVPSVAPSGLSGGGGAPGELTINWTPMSREYQNGDGFGYLLSFRRQGSSSWQTARVPGADT QYFVYSNDSIHPYTPFEVKIRSYNRRGDGPESLTAIVYSAEEEPKVAPAKVWAKGSSSSEMNVSWEPVLQ DMNGILLGYEIRYWKAGDKEAAADRVRTAGLDSSARVTGLYPNTKYHVTVRAYNRAGTGPASPSADAMTM KPPPRRPPGNISWTFSSSSLSLKWDPVVPLRNESTVTGYKMLYQNDLQPTPMLHLTSKNWIEIPVPEDIG HALVQIRTTGPGGDGIPAEVHIVRNGGTSMMVESSAVRPAHPGPVFSCMVILMLAGCQRL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC066106
ORF Size	3120 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC066106, AAH66106
RefSeq Size	5868 bp
RefSeq ORF	3122 bp
Locus ID	21367
Gene Summary	This gene encodes a member of the contactin family of proteins, part of the immunoglobulin superfamily of cell adhesion molecules. The encoded glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein plays a role in the proliferation, migration, and axon guidance of neurons of the developing cerebellum. Mice lacking a functional copy of this gene exhibit epileptic seizures and elevated expression of A1 adenosine receptors. [provided by RefSeq, Sep 2016]

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