Cobll1 (BC067007) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1810047P18Rik; Coblr1; D430044D16Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211472 representing BC067007 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCGCAGCGTCCCGGATCCCCTACCCCGCAGCGCGCCCCGCACCCCCGCCATGCAGCCCGCGGGCT CCGCAGGGAAAACAAAAGGGAAGGCGCCTCTTCCTCCGGCCGAGACGAAGGGCACTGATGTCTCTTCTGC TGAAGACCCTGTAGAATCTACTGCTGTCGTCACAGAGCAACAAGACAATATGATAGATAAAGATATTGAG CTCTCAGTGGTCCTGCCGGGAGATATCCTCAAGTCTACTACTGTTCATGGCAGTAAGCCAATGATGGACT TGCTCGTGTTCCTCTGTGCTCAGTATCACTTAAATCCGTCAAGTCACACAATTGATTTGCTCTCTGCTGA AGAGAACCTTATCAAATTTAAGCCAAACACACCAATAGGAATGCTGGATGTAGAGAAGGTAATTTTAAAG CCAAAATCTCTGGATAAGAAAAAGCCTACACCTATAATCCCAGAGAAAACTGTGAGAGTAGTGATCAACT TTAAGAAAACACAGAAGACCATAGTGAGAGTTAGTCCCCACGCACCCCTTCAAGATCTTGCTCCCATCAT ATGCAGTAAATGTGAGTTTGATCCGTTGCATACCGTGCTGTTGAAGGATTACCAGGCTCAGGAGCCCCTC GACTTGACAAAATCTCTTAATGACTTGGGACTAAGAGAGTTATATGCCATGGATATCAGCAGAGAGTCCT GCCAAATATCACACAACCCGGACATTGTGAAGGAGAAAGAAAACAAAGGGATTTTCAGTTTTTTCCAACG CAGCAAGAAAAAGCGAGAGCAAACTGCCAGTGCTCCTGCGACCCCACTAGTGAGCAAGCATCGTCCATCT TTCACGAGGTCCAACACCATTTCCAAACCGTACATTTCCAATACCCTGCCCTCGGATGCACCCAAGAAGA GACGTGCCCCACTGCCACCGATGCCCACATCCCAGGGTGCTGCTCAAGGCCAGGAGAGACGAGCGTCTTG TGTGGAGAGATCCACCAGTGTGGACGACACAGATAAGAGTTCCTCTGAAGCGATCATGGTGAGGACAGGA TCACTGCAGCTCAGCAGCACGTCGATAGGAACTTCATCTCTGAAGAGAACGAAGCGAAAAGCACCTGCCC CACCTTCGAAAACCCCCCTGGCTCAGACTGATGAAAGGAACTCTGCAATGGCTCATGGCCTACCTTTGGA AGATGGGATTGCTCCAGACAGCATGTTGGAACTGAGCTCTCCCGAGGGAATGTCCACCCCAGCTGGTGTA AGCTCTGATTACAGTCTTGAGGAGATAGATGAAAAGGAAGAACTCAGCGAGGCATCTAAAGATCCGGCTG GAAGTATTTCTGTGAAGTCGCCTGACATTGCTTCAGCATCGACTGATATGAGAATTACAGTGGAGAAGGA TCCTGATTCAGCCCTCGGCATCAGTGATGGAGAGACCTCTCCAAGCTCCAAGGGAAAAACTCAAGAAGGC AGAAGCACAGAGGGGCAAGGGCCGTATCATCCTGTTGTAGGCCATATAGGCAATGAGGACAGAGTATCTG ACAGTATAAAAGACATGAAGACACTGGGCCCAAACCAGGAGAGTGTAGTTCAAAATGAAATCATGGTCTG TGCCACAAGCACAGATTATGTGAAAAACAGACCGGGGAAAATGGAAACCACCATAGAAGGCGAAGGCGAA GCTCTGAAGAAAGCCAGCGACATGGAAACTGATAGATTGTCAGGCTCTCCTGCATGTAGAATGGACAATG TTAAAAGTTCGAGGGAAAATCATCTAACAGCATCGCCAGGACCAGATCAAAAATTGAATCAACCAGGTGT AGAGAAGACGAAAATGCAGGATGCAGCAATTCAGGCCACCCCTGCCCGCGGCACTTTTGATGGGAATCAC GAAGTCAGTAATTCATCTGACCCCAGGGCTGATGAGACTGTGCAGACTTCGGATGGCAGCATATCAGCTC AGCACTCCTCTGCAAGTCTCCAGGATTCTGTAAATGCCTCAAGAGAATTCAGGAGTCAGGGCACCTCAAC TTATGTACAAGATAGACTCCCTGAGAAAGAGCCAGCTTGTACATATGGGAACAATGTCCCTTTGTCACCT GTAGATGGAAGTAATAAGAATCCAGCTGCTTCTTACCTAAAGAACTTTCCACTTTATAGGCAAGACTCCA ACCCCAAGCCAAAACCGTCAAATGAAATCACGAGAGAGTATATACCTAAAATTGGAATGACTACTTATAA AATAGTACCTCCGAAATCCCTGGAAATGGCGAAAGACTGGGAATCAGAAGCAATGGGGAGGAAAGATGAC CAAAAGATGCTCCCTGTAGGGCAGAGGCATACGATTGAGAATATGACAGAAACTTCCATGCAAACAGAAG TCCCTGCTACTTCTAAAAGCTCACAGCAGCCTCAGCCTGACCTTAAACCAAAGCCCAGCTCAGGAACAGA GCGTCATTTGCACAGGACTTTGAGCTCACCAACTGGTACCGAGACGAACCCTCCCAAAGCACCCAGAGTG ACTACAGACACTGGCACCATTCCTTTTGCGCCAAATTTGGAAGATATAAACAATATTTTGGAGTCAAAAT TTAGATCCCGAGCTTCAAATCCCCAGGCCAAACCAAGTTCATTCTTTTTGCAGATGCAAAAGAGAGCATC AGGCCACTATGTGACATCTGCAGCTGCTAAGAGCGTCCACACTGCCCCTGGGCCTGCCCCTAAAGAGCCA ACAATCAAGGAGGTGCAGAGGGACCCACAGCTTTCTCCAGAACAGCATCCTTCTTCCTTAAGTGAAAGGA CTCACTCTGCTCCGCTGCCCAATATTTCAAAAGCTGATGATGATATAATTCAGAAGCCTGCCGAGACCTC TCCTCCTCCTGTAGCTCCCAAACCAATGACTCTTCGTGCCGAGACCTCTCCTCCTCCTGTATTTCCCAAA CCAATGACTCTTCCTGCTGAGACCTCTCCTCCTCCTGTATTTCCCAAACCAATGACTCTTCCTGCCGAGA CCTCTCTTCCTCTTGTATTTCCCAAACCAATGACTCTTCGAGCCGAGACCTCTCCTCCTCCTCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211472 representing BC067007 Red=Cloning site Green=Tags(s) MDRSVPDPLPRSAPRTPAMQPAGSAGKTKGKAPLPPAETKGTDVSSAEDPVESTAVVTEQQDNMIDKDIE LSVVLPGDILKSTTVHGSKPMMDLLVFLCAQYHLNPSSHTIDLLSAEENLIKFKPNTPIGMLDVEKVILK PKSLDKKKPTPIIPEKTVRVVINFKKTQKTIVRVSPHAPLQDLAPIICSKCEFDPLHTVLLKDYQAQEPL DLTKSLNDLGLRELYAMDISRESCQISHNPDIVKEKENKGIFSFFQRSKKKREQTASAPATPLVSKHRPS FTRSNTISKPYISNTLPSDAPKKRRAPLPPMPTSQGAAQGQERRASCVERSTSVDDTDKSSSEAIMVRTG SLQLSSTSIGTSSLKRTKRKAPAPPSKTPLAQTDERNSAMAHGLPLEDGIAPDSMLELSSPEGMSTPAGV SSDYSLEEIDEKEELSEASKDPAGSISVKSPDIASASTDMRITVEKDPDSALGISDGETSPSSKGKTQEG RSTEGQGPYHPVVGHIGNEDRVSDSIKDMKTLGPNQESVVQNEIMVCATSTDYVKNRPGKMETTIEGEGE ALKKASDMETDRLSGSPACRMDNVKSSRENHLTASPGPDQKLNQPGVEKTKMQDAAIQATPARGTFDGNH EVSNSSDPRADETVQTSDGSISAQHSSASLQDSVNASREFRSQGTSTYVQDRLPEKEPACTYGNNVPLSP VDGSNKNPAASYLKNFPLYRQDSNPKPKPSNEITREYIPKIGMTTYKIVPPKSLEMAKDWESEAMGRKDD QKMLPVGQRHTIENMTETSMQTEVPATSKSSQQPQPDLKPKPSSGTERHLHRTLSSPTGTETNPPKAPRV TTDTGTIPFAPNLEDINNILESKFRSRASNPQAKPSSFFLQMQKRASGHYVTSAAAKSVHTAPGPAPKEP TIKEVQRDPQLSPEQHPSSLSERTHSAPLPNISKADDDIIQKPAETSPPPVAPKPMTLRAETSPPPVFPK PMTLPAETSPPPVFPKPMTLPAETSLPLVFPKPMTLRAETSPPPL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC067007
ORF Size	3077 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC067007, AAH67007
RefSeq Size	3983 bp
RefSeq ORF	3077 bp
Locus ID	319876

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