Tbc1d9 (BC057027) Mouse Tagged ORF Clone

CAT#: MG211436

  • TrueORF®

Tbc1d9 (tGFP-tagged) - Mouse TBC1 domain family, member 9 (cDNA clone MGC:67187 IMAGE:6825869)

ORF Plasmid: DDK tGFP



  "BC057027" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 11,120.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 4933431N12Rik; AI847101; AW490653; C76116
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211436 representing BC057027
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGATTTGAACAGGACCGGTCCCTGCCTAAACTCAAGAAGAAATCTCCCAAAAAAAGTATCTGCTC
TGAAACGTGATCTGGATGCCAGAGCGAAGAGCGAGCGATACCGCGCCCTTTTCCGGCTGCCCAAAGACGA
AAAGCTAGACGGGCACACTGACTGCACCCTCTGGACTCCATTTAACAAGATGCACATTTTGGGTCAGATG
TTCGTCTCCACTAATTACATCTGTTTTACCAGCAAGGAGGAAAACCTCTGCAGCCTCATAATTCCACTCC
GGGAAGTAACCATCGTGGAGAAGGCCGACAGCTGCAGTGTGCTCCCCAGCCCTCTGTCCATCAGCACCAG
GAACAGGATGACCTTCCTGTTCGCGAACTTGAAAGACAGAGACTTTCTGGTGCAGAGGATCTCAGATTTC
TTGCAGCAAACGACTTCTAGGATCTACTCAGACAAAGAGTTTTCAGGAAGCTGCAACAGCTCAGATGATG
AGGTGTATTCTCGGCCCAGCAGCCTCGTTTCCTCCAGTCCCCAGAGAAGCACAAGCTCGGATGCTGATGG
AGAACGCCCCTTTAACCTCAATGGCAACAGTGTCCCTACAGCCACCCAGACCCTCATGACCATGTACCGG
CGGCGATCTCCTGAAGAATTCAACCCAAAATTGGCCAAAGAGTTTTTGAAAGAACAAGCCTGGAAGATTC
ACTTTGCTGAGTATGGGCAGGGAATCTGCATGTACCGCACAGAGAAGACCCGGGAGCTGGTGCTGAAGGG
CATCCCAGAGAGCATGCGCGGAGAGCTCTGGCTGCTGCTTTCAGGGGCCATCAATGAAAAGGCCACACAC
CCCGGGTACTACGAGGGCCTCGTGGAGAAGTCGATGGGAAAGTACAATCTGGCCACCGAGGAGATTGAGA
GAGACTTACACCGCTCCCTTCCAGAACACCCAGCTTTCCAGAACGAAATGGGCATCGCGGCTCTGAGGAG
AGTCTTAACGGCTTATGCTTTTCGCAATCCCAACATAGGATACTGCCAGGCCATGAACATTGTCACTTCA
GTGCTACTGCTGTATGCCAAAGAGGAGGAGGCCTTCTGGCTGCTTGTGGCCTTGTGTGAGCGCATGCTTC
CCGACTACTACAACACCAGAGTTGTAGGTGCCCTGGTGGACCAGGGAGTCTTCGAGGAGCTGGCTCGAGA
CTATGTCCCGCAGCTGTACGACTGCATGCAGGACCTGGGGGTGATCTCTACCATCTCCCTGTCGTGGTTC
CTCACACTCTTCCTCAGTGTGATGCCATTTGAGAGTGCAGTGGTGGTTGTGGACTGTTTCTTCTATGAAG
GAATCAAAGTGATATTTCAGCTGGCTCTCGCTGTACTGGATGCCAACGTGGATAAACTCTTGAACTGCAA
GGACGATGGAGAGGCCATGACTGTTTTGGGAAGGTACCTAGACAGTGTGACCAACAAAGACAGCACCCTG
CCTCCCATCCCCCATCTCCACTCCCTGCTCAGTGACGACGTGGGGCCCTACCCTGCCGTGGACATCTTCA
GACTCATAGGGACGTCTTACGAGAAATTTGGAACTATCCGGGCCGATTTGATTGAGCAGATGCGATTCAA
GCAGAGGCTGAAAGTGATCCAGACCCTGGAGGACACCACCAAACGCAATGTGGTACGGACCATTGTGACG
GAGACGTCCTTCACCATCGATGAGCTGGAAGAACTGTACGCTCTTTTCAAGGCTGAGCATCTGACCAGCT
GCTACTGGGGTGGGAGCAGCAATGCCCTGGACCGACACGACCCCAGCCTGCCTTACCTGGAGCAGTACCG
CATCGACTTTGAGCAGTTCAAGGGCATGTTCGTGCTGCTCTTTCCCTGGGCGTGTGGCACACACTCCGAT
GTGCTGGCCTCCCGCCTGTTCCAGCTGTTAGATGAGAATGGAGACTCCTTGATTAACTTCCGAGAGTTCG
TCTCTGGGCTGAGTGCTGCATGCCATGGGGACCTCACAGAGAAACTCAAACTCCTGTACAAGATGCACGT
CTTGCCTGAGCCATCCTGTGATCAAGATGAGCCTGACTCCGCCTTTGAAGCCACTCAGTACTTTTTTGAA
GACATCACCCCAGAATGCACACATGTTGTTGGCTTGGACAGTAGAGGCAAGCAGAGTGCGGACGACGGCT
TCGTCACAGTGAGCCTGAAGCAGGACAGAGGGAAAAGGGCAAATTCTCAAGAAAACCGTAATTACCTAAA
ACTCTGGACTGCAGAAAATAAGTCTAAGTCAAAAACTGCCAAGGATTTGCCCAAGCTGAACCAGGGGCAG
TTTATTGAGCTCTGTAAGACCATGTACAACATGTTCAGTGAAGACCCCAATGAGCAGGAGCTGTACCATG
CCACAGCTGCGGTGACCAGCCTCCTGCTGGAGATCGGAGAGGTTGGCAAGTTCTTCATTACTCAGCCGGC
AAAGGAAGATGCAGTTCCTGGACCACCCTGCGGCCAGGCCATCCCAGGCATGCTCTTCCCTAAGAAGGGG
TCCAGCCAGTCCTATGTGGTGGAGTCCACAGAGCCACTCACAGCTAGCCTGGCAGTGGACAGTGAGGAGC
ACTCCCTGGGAGGGCAGATGGAGGACATTAAACTGGAGGACTCCTCACCCAGGGACAACGGGGCCTGCTC
CTCCATGTTGATCTCCGATGATGACACCAAGGATGACAGCTCCATGTCATCCTACTCGGTGCTGAGCGCC
GGCTCCCACGAGGAGGACAAACTGCACTGTGAGGACATTGGAGAGGACACAGTCCTGGTGCGTAGCAGTC
AGGGTAGAGCCACCCTGCCCAGGAGCAGCAGCCTGGACCGGGACTGGGCCATCACCTTTGAGCAGTTCCT
GGCCTCGCTCTTGACGGAGCCCGCTCTCGTGAGGTACTTTGACAAGCCAGTGTGCATGATGGCCAGGGTC
ACCAGTGCGAAGAACATCCGTATGATGGGCAAGCCCCTCACCTCAGCCAGTGACTATGAAATCTCTGCCC
TGTCGGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211436 representing BC057027
Red=Cloning site Green=Tags(s)

MKDLNRTGPCLNSRRNLPKKVSALKRDLDARAKSERYRALFRLPKDEKLDGHTDCTLWTPFNKMHILGQM
FVSTNYICFTSKEENLCSLIIPLREVTIVEKADSCSVLPSPLSISTRNRMTFLFANLKDRDFLVQRISDF
LQQTTSRIYSDKEFSGSCNSSDDEVYSRPSSLVSSSPQRSTSSDADGERPFNLNGNSVPTATQTLMTMYR
RRSPEEFNPKLAKEFLKEQAWKIHFAEYGQGICMYRTEKTRELVLKGIPESMRGELWLLLSGAINEKATH
PGYYEGLVEKSMGKYNLATEEIERDLHRSLPEHPAFQNEMGIAALRRVLTAYAFRNPNIGYCQAMNIVTS
VLLLYAKEEEAFWLLVALCERMLPDYYNTRVVGALVDQGVFEELARDYVPQLYDCMQDLGVISTISLSWF
LTLFLSVMPFESAVVVVDCFFYEGIKVIFQLALAVLDANVDKLLNCKDDGEAMTVLGRYLDSVTNKDSTL
PPIPHLHSLLSDDVGPYPAVDIFRLIGTSYEKFGTIRADLIEQMRFKQRLKVIQTLEDTTKRNVVRTIVT
ETSFTIDELEELYALFKAEHLTSCYWGGSSNALDRHDPSLPYLEQYRIDFEQFKGMFVLLFPWACGTHSD
VLASRLFQLLDENGDSLINFREFVSGLSAACHGDLTEKLKLLYKMHVLPEPSCDQDEPDSAFEATQYFFE
DITPECTHVVGLDSRGKQSADDGFVTVSLKQDRGKRANSQENRNYLKLWTAENKSKSKTAKDLPKLNQGQ
FIELCKTMYNMFSEDPNEQELYHATAAVTSLLLEIGEVGKFFITQPAKEDAVPGPPCGQAIPGMLFPKKG
SSQSYVVESTEPLTASLAVDSEEHSLGGQMEDIKLEDSSPRDNGACSSMLISDDDTKDDSSMSSYSVLSA
GSHEEDKLHCEDIGEDTVLVRSSQGRATLPRSSSLDRDWAITFEQFLASLLTEPALVRYFDKPVCMMARV
TSAKNIRMMGKPLTSASDYEISALSG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC057027
ORF Size 3018 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC057027.1
RefSeq Size 4453 bp
RefSeq ORF 3020 bp
Locus ID 71310
Gene Summary May act as a GTPase-activating protein for Rab family protein(s).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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