Gtf2i (BC053044) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	TFII-I, WBSCR6, BAP-135, GtfII-I, Spin
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211413 representing BC053044 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAAGTAGTGATGTCTGCCTTGCCTGCCGAAGATGAAGAGTCTTCAGAGAGCAGGATGGTGGTGA CCTTCCTCATGTCTGCCCTGGAGTCCATGTGTAAAGAGCTGGCCAAGTCCAAGGCTGAGGTGGCCTGCAT TGCTGTGTATGAGACCGACGTGTTTGTTGTTGGAACCGAAAGAGGGCGTGCTTTTGTCAATACCAGAAAA GATTTCCAAAAAGACTTTGTGAAGTATTGTGTTGAAGAAGAAGAAAAGGCCGCCGAGATGCATAAGATGA AATCTACCACCCAGGCCAATCGGATGAGTGTGGATGCTGTAGAAATTGAAACACTCAGAAAAACGGTGGA GGACTATTTCTGTTTTTGCTATGGGAAAGCTTTAGGCAAATCCACAGTGGTCCCTGTGCCATATGAGAAG ATGCTGCGCGACCAGTCGGCTGTGGTGGTGCAGGGGCTTCCCGAGGGGGTTGCCTTCAAGCACCCCGAAC ATTACGACCTCGCAACCTTGAAGTGGATTTTGGAGAACAAGGCAGGGATTTCCTTCATCATTAAGAGACC TTTCCTCGAGCCGAAGAAACACCTCGGTGGTCGAGTGCTGGCGGCCGAGGCTGAGAGGTCCATGCTGTCT CCTAGTGGAAGTTGTGGCCCCATCAAAGTGAAAACTGAACCCACAGAAGATTCTGGCATTTCTCTGGAAA TGGCCGCTGTGACAGTGAAGGAGGAGTCAGAAGACCCTGATTACTATCAGTATAACATTCAAGGCCCTTC TGAAACTGATGGTGTTGATGAAAAGCTCCCCCTTTCGAAGGCTTTGCAAGGAAGCCATCACTCCTCAGAA GGCAACGAGGGAACGGAAGTGGAAGTGCCAGCAGAAGATTCTACTCAACATGTCCCTTCAGAAACCAGTG AGGACCCCGAAGTTGAGGTGACCATTGAAGATGATGACTATTCTCCACCCACCAAGAGGCTAAAGAGCAC GGAGCCGCCCCCGCCCCCGCCCGTCCCGGAGCCCGCCAACGCTGGCAAGCGCAAAGTGAGGGAGTTCAAC TTTGAGAAATGGAACGCACGCATCACTGACCTACGGAAACAAGTTGAAGAGTTGTTCGAAAGAAAATATG CTCAAGCCATCAAAGCCAAAGGCCCGGTGACGATCCCGTACCCTCTTTTCCAGTCCCATGTTGAAGATCT TTACGTAGAAGGGCTTCCTGAAGGGATTCCTTTTAGAAGGCCGTCGACGTACGGCATTCCTCGCCTTGAG AGGATATTGCTGGCGAAGGAAAGGATCCGCTTCGTGATTAAGAAACATGAGCTTCTGAACTCAACACGAG AAGATTTACAGCTTGATAAACCAGCCTCAGGAGTGAAAGAAGAGTGGTACGCTCGCATCACCAAACTGAG GAAGATGGTTGACCAGCTCTTCTGCAAGAAATTTGCCGAGGCCTTGGGAAGCACGGAAGCCAAGGCTGTA CCGTACCAAAAATTTGAGGCCCATCCCAATGATCTCTATGTGGAAGGACTTCCAGAAAACATTCCTTTCC GAAGCCCCTCGTGGTATGGAATCCCAAGACTGGAAAAAATCATCCAAGTGGGCAATCGAATTAAATTTGT TATCAAGAGACCAGAGCTGCTCACTCACAGCACAACTGAAGTGACTCAGCCACGGACAAACACACCAGTC AAAGAAGATTGGAATGTCAGAATCACCAAGCTCCGAAAGCAAGTGGAAGAGATCTTTAATTTGAAGTTTG CTCAGGCTCTGGGCCTCACAGAGGCTGTGAAGGTGCCATATCCTGTGTTCGAATCCAACCCGGAGTTTCT GTATGTGGAAGGGCTCCCCGAAGGGATCCCCTTCCGAAGCCCTACCTGGTTTGGGATTCCACGCCTCGAG AGGATTGTCCGTGGCAGCAATAAGATCAAGTTTGTTGTTAAAAAGCCTGAGCTAGTTGTGTCCTATTTGC CTCCTGGGATGGCTAGCAAAATCAACACTAAAGCATTGCAGTCCCCAAAACGACCACGAAGCCCTGGGAG CAACTCCAAGGTTCCTGAAATTGAGGTCACTGTGGAAGGCCCCAACAACAGCAGTCCTCAGACCTCTGCG GTTCGAACTCCTACCCAGACCAATGGTTCAAATGTTCCCTTCAAGCCTCGAGGGAGAGAGTTTTCCTTTG AGGCCTGGAATGCTAAGATCACAGACCTGAAGCAGAAAGTGGAGAACCTCTTCAATGAAAAGTGCGGGGA AGCGCTTGGCCTTAAGCAGGCTGTGAAAGTCCCGTTTGCGTTGTTCGAGTCCTTCCCAGAAGACTTCTAT GTGGAAGGCTTGCCCGAGGGGGTGCCCTTCCGAAGGCCGTCCACATTTGGCATTCCCAGGCTGGAGAAGA TACTCCGGAACAAGGCCAAGATAAAGTTCATCATTAAAAAACCTGAGATGTTCGAGACGGCCATCAAAGA GAGCACCTCTTCCAAAAGCCCTCCAAGAAAGATAAACTCATCACCCAACGTTAATACTACTGCATCAGGT GTGGAAGACCTGAACATCATCCAGGTGACAATTCCAGATGACGATAATGAACGACTGTCCAAAGTTGAAA AGGCCAGGCAGCTGCGGGAGCAGGTCAACGACCTCTTCAGTCGGAAGTTTGGTGAAGCTATTGGGATGGG CTTCCCGGTGAAGGTCCCCTACAGGAAGATCACCATCAACCCTGGCTGCGTGGTGGTCGATGGCATGCCC CCCGGGGTGTCCTTCAAAGCCCCCAGTTACCTGGAGATCAGCTCCATGAGGAGGATCTTAGACTCTGCGG AGTTTATCAAGTTCACTGTCATTAGACCATTTCCAGGACTTGTGATTAATAACCAGCTGGTTGATCAGAA CGAGTCTGAAGGCCCTGTGATACAAGAATCAGCCGAGGCAAGCCAGTTGGAAGTCCCAGTAACAGAAGAA ATTAAAGAGACAGATGGGAGTTCACAGATCAAGCAGGAGCCAGACCCCACGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211413 representing BC053044 Red=Cloning site Green=Tags(s) MAQVVMSALPAEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRK DFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEK MLRDQSAVVVQGLPEGVAFKHPEHYDLATLKWILENKAGISFIIKRPFLEPKKHLGGRVLAAEAERSMLS PSGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGPSETDGVDEKLPLSKALQGSHHSSE GNEGTEVEVPAEDSTQHVPSETSEDPEVEVTIEDDDYSPPTKRLKSTEPPPPPPVPEPANAGKRKVREFN FEKWNARITDLRKQVEELFERKYAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLE RILLAKERIRFVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAV PYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPV KEDWNVRITKLRKQVEEIFNLKFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLE RIVRGSNKIKFVVKKPELVVSYLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNSSPQTSA VRTPTQTNGSNVPFKPRGREFSFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFY VEGLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKINSSPNVNTTASG VEDLNIIQVTIPDDDNERLSKVEKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINPGCVVVDGMP PGVSFKAPSYLEISSMRRILDSAEFIKFTVIRPFPGLVINNQLVDQNESEGPVIQESAEASQLEVPVTEE IKETDGSSQIKQEPDPTW TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC053044
ORF Size	2996 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC053044, AAH53044
RefSeq Size	4461 bp
RefSeq ORF	2996 bp
Locus ID	14886
Gene Summary	Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box (By similarity). Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.[UniProtKB/Swiss-Prot Function]

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