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Epha7 (BC026153) Mouse Tagged ORF Clone
CAT＃: MG211406
- TrueORF®
Epha7 (tGFP-tagged) - Mouse Eph receptor A7 (cDNA clone MGC:14056 IMAGE:3991628)
ORF Plasmid: DDK
"BC026153" in other vectors (4)
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CNY 10,830.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ehk3, Mdk1, Cek11, Hek11 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211406 representing BC026153
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGTTCAAACTCGGTTCCCTTCGTGGATTATTTTGTGTTACATCTGGCTGCTTGGCTTTGCACACA CGGGGGAGGCGCAGGCTGCGAAGGAAGTACTATTACTGGACTCGAAAGCACAACAAACAGAATTGGAATG GATTTCCTCTCCACCCAGTGGGTGGGAAGAAATTAGTGGTTTGGATGAGAACTACACTCCGATAAGAACA TACCAGGTGTGCCAGGTCATGGAGCCCAACCAGAACAACTGGCTGCGGACTAACTGGATTTCTAAAGGCA ACGCACAAAGGATTTTTGTAGAATTGAAATTCACCTTGAGGGATTGTAATAGTCTTCCCGGAGTCCTGGG AACTTGCAAGGAAACGTTTAATTTGTACTATTATGAAACAGACTACGACACCGGCAGGAATATACGAGAA AACCTTTATGTTAAAATAGACACCATTGCTGCAGATGAAAGTTTCACACAAGGTGACCTTGGTGAAAGAA AGATGAAGCTGAACACTGAGGTGAGAGAGATTGGACCTTTGTCCAAAAAGGGATTCTATCTTGCCTTTCA GGATGTAGGGGCTTGCATAGCATTGGTTTCTGTCAAAGTGTACTACAAGAAGTGCTGGTCCATTGTTGAG AACTTAGCTGTCTTTCCAGATACAGTGACTGGTTCGGAATTTTCGTCCTTAGTCGAGGTCCGTGGGACAT GTGTCAGCAGTGCCGAGGAAGAGGCAGAAAATTCCCCCAGGATGCATTGCAGTGCAGAAGGAGAGTGGCT AGTACCCATTGGAAAATGCATCTGCAAAGCAGGCTATCAGCAAAAAGGGGACACTTGCGAACCCTGTGGC CGCAGGTTCTACAAATCTTCCTCTCAGGATCTCCAGTGTTCTCGTTGTCCAACCCACAGCTTCTCTGACC GAGAAGGATCATCCAGGTGTGAATGTGAAGATGGGTACTACAGAGCTCCTTCTGATCCACCATACGTTGC ATGCACGAGGCCTCCCTCTGCACCACAGAACCTTATTTTCAACATCAATCAAACGACTGTAAGTTTGGAA TGGAGTCCTCCGGCTGACAACGGGGGAAGAAACGATGTCACCTACAGAATACTGTGTAAGCGGTGCAGTT GGGAACAGGGAGAATGTGTGCCATGCGGAAGTAACATTGGATACATGCCCCAGCAGACAGGATTAGAGGA TAACTATGTCACTGTCATGGACCTACTTGCCCATGCAAATTACACTTTCGAAGTTGAAGCTGTAAATGGA GTTTCGGACTTAAGCAGATCCCAGAGGCTCTTCGCTGCAGTTAGCATCACTACCGGTCAAGCAGCTCCCT CGCAAGTGAGTGGAGTCATGAAGGAGCGAGTACTGCAGCGGAGTGTGCAGCTTTCCTGGCAGGAGCCGGA GCATCCCAATGGAGTCATCACCGAATATGAAATCAAGTATTATGAGAAAGATCAACGGGAAAGGACGTAC TCAACACTCAAAACCAAGTCCACCTCCGCCTCCATTAATAATCTGAAACCGGGAACAGTGTATGTGTTTC AGATCCGGGCGGTCACTGCTGCCGGTTATGGAAACTACAGCCCTAGGCTTGATGTTGCCACACTTGAGGA AGCTTCAGGTAAAATGTTTGAAGCGACAGCAGTCTCCAGTGAACAGAATCCTGTCATCATAATTGCTGTA GTGGCTGTAGCAGGGACCATCATCTTGGTGTTCATGGTGTTCGGCTTCATCATTGGAAGAAGGCACTGTG GTTATAGCAAGGCTGACCAAGAAGGGGATGAAGAACTCTACTTTCATTGCACCAAAACCTACATTGACCC TGAAACCTATGAGGACCCAAATAGAGCTGTCCATCAATTCGCCAAGGAGCTAGATGCCTCCTGTATTAAA ATTGAGCGTGTGATTGGTGCAGGAGAATTTGGAGAAGTTTGCAGTGGTCGTTTGAAACTTCCGGGGAAGA GAGATGTTGCAGTGGCCATAAAAACCCTGAAAGTTGGTTACACAGAAAAACAAAGGAGGGACTTTTTATG CGAAGCAAGCATCATGGGGCAATTTGACCACCCAAATGTCGTCCATTTGGAAGGGGTTGTTACAAGAGGG AAGCCTGTCATGATTGTGATAGAGTTCATGGAGAATGGAGCCCTGGATGCATTTCTCAGGAAACACGATG GGCAGTTTACAGTCATTCAGTTGGTAGGAATGCTGAGAGGTATTGCCGCTGGGATGCGATACTTGGCTGA TATGGGATACGTTCACAGGGACCTTGCAGCACGCAACATCCTTGTCAACAGCAATCTTGTTTGTAAAGTG TCAGATTTTGGCCTTTCCCGGGTTATAGAGGATGATCCCGAAGCTGTCTACACCACGACTGGTGGAAAAA TTCCAGTAAGGTGGACTGCACCGGAAGCCATTCAATACCGGAAGTTCACCTCAGCCAGCGATGTGTGGAG CTATGGGATTGTCATGTGGGAAGTGATGTCTTATGGAGAAAGACCTTACTGGGACATGTCAAATCAAGAT GTCATTAAAGCGATAGAAGAAGGTTATCGTTTGCCGGCGCCCATGGATTGCCCAGCTGGTCTTCACCAGC TAATGCTGGATTGTTGGCAGAAAGATCGGGCGGAAAGGCCAAAGTTTGAGCAGATAGTCGGAATTCTAGA CAAAATGATTCGAAACCCAAGTAGTCTGAAAACACCCCTGGGAACTTGTAGTAGACCCATAAGCCCTCTT CTGGACCAGAGCACTCCTGACTTCACTGCCTTCTGTTCAGTTGGAGAATGGTTGCAAGCTATTAAAATGG AAAGGTATAAGGACAACTTCACAGCAGCGGGTTACAACTCACTCGAGTCAGTGGCCAGGATGACTATCGA TGATGTGATGAGTTTAGGGATCACACTGGTTGGCCATCAAAAGAAGATCATGAGCAGCATCCAGACTATG CGGGCACAAATGTTGCATTTACACGGAACAGGCATCCAAGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211406 representing BC026153
Red=Cloning site Green=Tags(s) MVVQTRFPSWIILCYIWLLGFAHTGEAQAAKEVLLLDSKAQQTELEWISSPPSGWEEISGLDENYTPIRT YQVCQVMEPNQNNWLRTNWISKGNAQRIFVELKFTLRDCNSLPGVLGTCKETFNLYYYETDYDTGRNIRE NLYVKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSKKGFYLAFQDVGACIALVSVKVYYKKCWSIVE NLAVFPDTVTGSEFSSLVEVRGTCVSSAEEEAENSPRMHCSAEGEWLVPIGKCICKAGYQQKGDTCEPCG RRFYKSSSQDLQCSRCPTHSFSDREGSSRCECEDGYYRAPSDPPYVACTRPPSAPQNLIFNINQTTVSLE WSPPADNGGRNDVTYRILCKRCSWEQGECVPCGSNIGYMPQQTGLEDNYVTVMDLLAHANYTFEVEAVNG VSDLSRSQRLFAAVSITTGQAAPSQVSGVMKERVLQRSVQLSWQEPEHPNGVITEYEIKYYEKDQRERTY STLKTKSTSASINNLKPGTVYVFQIRAVTAAGYGNYSPRLDVATLEEASGKMFEATAVSSEQNPVIIIAV VAVAGTIILVFMVFGFIIGRRHCGYSKADQEGDEELYFHCTKTYIDPETYEDPNRAVHQFAKELDASCIK IERVIGAGEFGEVCSGRLKLPGKRDVAVAIKTLKVGYTEKQRRDFLCEASIMGQFDHPNVVHLEGVVTRG KPVMIVIEFMENGALDAFLRKHDGQFTVIQLVGMLRGIAAGMRYLADMGYVHRDLAARNILVNSNLVCKV SDFGLSRVIEDDPEAVYTTTGGKIPVRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQD VIKAIEEGYRLPAPMDCPAGLHQLMLDCWQKDRAERPKFEQIVGILDKMIRNPSSLKTPLGTCSRPISPL LDQSTPDFTAFCSVGEWLQAIKMERYKDNFTAAGYNSLESVARMTIDDVMSLGITLVGHQKKIMSSIQTM RAQMLHLHGTGIQV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC026153 |
| ORF Size | 2982 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026153.1 |
| RefSeq Size | 3554 bp |
| RefSeq ORF | 2984 bp |
| Locus ID | 13841 |
| Gene Summary | Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7. Isoform 4 which lacks the kinase domain may regulate isoform 1 adhesive properties.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220717 | Epha7 (untagged) - Mouse Eph receptor A7 (cDNA clone MGC:14056 IMAGE:3991628), (10ug) |
CNY 9,120.00 |
|
| MR211406 | Epha7 (Myc-DDK-tagged) - Mouse Eph receptor A7 (cDNA clone MGC:14056 IMAGE:3991628) |
CNY 9,880.00 |
|
| MR211406L3 | Lenti ORF clone of Epha7 (Myc-DDK-tagged) - Mouse Eph receptor A7 (cDNA clone MGC:14056 IMAGE:3991628) |
CNY 11,780.00 |
|
| MR211406L4 | Lenti ORF clone of Epha7 (mGFP-tagged) - Mouse Eph receptor A7 (cDNA clone MGC:14056 IMAGE:3991628) |
CNY 11,780.00 |
