Dlgap1 (BC062120) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Sapap1, GKPA/SAPAP, 9630002F18, mKIAA4162
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211400 representing BC062120 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGGGTTATCGGGTAGCCGCAGCCACCACCATGGGATCACCTGCGAGGCGGCCTGTGACTCCTTGT CACACCACTCAGACCACAAGCCGTACCTGCTGAGCCCCGTAGACCACCACCCTGCGGACCATCCCTACTA CACCCAGCGGAATTCTTTCCAGGCCGAGTGCGTAGGGCCCTTCAGCGACCCGCTGGCCAGCAGCACCTTC CCGCGCAGGCACTACACCTCGCAGCAGGAGCTGAAGGACGAGAGTGCCCTGGTACCACGCACCCTGGCCA CCAAGGCGAATCGTCTCCCCACCAACCTGCTGGATCAGTTTGAGAGGCAGCTGCCCCTCAGCCGGGATGG CTATCACACGCTACAGTACAAGCGCACCGCTGTGGAGCATCGCAGCGATAGCCCCGGCCGCATCCGGCAC CTGGTCCACTCGGTCCAGAAGCTCTTTACTAAGTCTCATTCCCTGGAGGGGCCATCCAAAGGCAGCGTCA ACGGGGGCAAGGCTAGCCCTGACGAGTCGCAAACTCTGAGATATGGCAAGCGCAGCAAGAGCAAGGAGAG GCGATCAGAATCCAAGGCTAGATCCAATGCTTCTAACGCCTCCCCCACCTCTCCCAGTTGGTGGAGTTCA GATGACAACCTGGATGGTGACATGTGCCTCTACCACACGCCCTCGGGTGTGATGACCATGGGCAGGTGTC CTGACCGCTCCGCTTCGCAGTACTTCATGGAAGCCTACAACACCATCAGCGAGCAGGCAGTGAAGGCTTC CCGGAGTAACAATGACATCAAGTGCTCCACCTGTGCCAACCTGCCAGTCACGCTGGATGCCCCGCTTCTG AAGAAGAGTGCCTGGTCCTCCACTCTCACCGTGAGCAGAGCCCGAGAGGTTTATCAGAAAGCCTCGGTGA ACATGGATCAGGCCATGGTGAAGTCCGAAGCTTGTCAACAAGAGCGGTCCTGCCAGTACTTACAGGTTCC CCAGGATGAATGGTCAGGGTACACACCTCGCGGGAAAGACGACGAGATCCCGTGCCGGAGGATGCGCAGT GGTAGCTACATCAAAGCCATGGGGGATGAAGACAGTGGGGACTCGGACACGAGCCCCAAGCCGTCTCCCA AGGTGGCTGCAAGAAGAGAAAGCTATCTCAAGGCCACGCAGCCGTCCCTTACAGAGCTCACCACTCTCAA GATCTCCAATGAACACTCCCCCAAACTCCAGATCCGGAGTCACAGCTACCTGAGGGCCGTAAGTGAAGTC TCCATCAACAGAAGCCTGGACAGCCTGGACCCTGCAGGCTTGCTCACGTCACCAAAGTTCCGCTCCAGAA ACGAGAGCTACATGCGCGCCATGAGCACCATAAGTCAGGTGAGCGAGATGGAGGTGAACGGCCAGTTCGA ATCCGTGTGCGAGTCTGTGTTCAGCGAGCTGGAGTCGCAGGCGGTGGAAGCGCTGGACCTGCCCCTGCCT GGCTGCTTCCGCATGCGGAGCCACAGCTATGTGCGGGCCATCGAAAAGGGCTGCTCCCAGGACGACGAAT GCGTGTCGCTGCGGTCGTCCTCCCCTCCACGCACAACCACCACCGTGAGGACCATCCAGAGCAGCACCGG TGTCATAAAACTGAGTTCTGCCGTTGAAGTGTCATCTTGCATTACAACATATAAGAAGACACCACCTCCA GTCCCACCCAGAACTACCACGAAACCTTTCATTTCTATCACAGCCCAGAGTAGCACAGAGTCTGCGCAGG ATGCCTACATGGACGGGCAAGGCCAGCGCGGAGACATGATCAGCCAGTCTGGCCTCAGCAACTCCACCGA AAGTCTGGACAGTATGAAGGCTCTCACGGCTGCCATCGAGGCTGCAAACGCCCAGATTCACGGCCCAGCA AGTCAACATATGGGCAGCAATGCTGCTGCTGTCACCACCACCACCACCATAGCCACTGTCACCACCGAGG ATAGGAAGAAAGACTTTAAGAAAAACCGATGTCTGTCTATTGGGATACAGGTGGACGATGCCGAAGAGCC GGAGAAAATGGCAGAGAGTAAGACGTCCAATAAGTTCCAGTCCGTGGGAGTGCAAGTAGAAGAAGAGAAG TGCTTCCGCAGGTTCACTCGGTCCAACAGTGTGACAACTGCAGTACAGGCCGACCTGGACTTCCATGATA ATCTGGAAAATTCTCTGGAGTCTATAGAGGACAATTCGTGTCCTGGCCCCATGGCCAGACAGTTCTCGCG GGACGCCAGCACCTCTACCGTCAGTATCCAGGGTTCAGGAAACCACTATCATGCCTGTGCAGCCGATGAC GACTTTGACACGGATTTTGACCCCTCTATCCTGCCTCCTCCGGACCCCTGGATTGACTCTATCACAGAAG ACCCTCTGGAGGCCGTTCAAAGGTCCGTGTGCCACCGGGACGGCCACTGGTTCCTGAAGCTTCTCCAGGC AGAGCGGGACCGCATGGAAGGGTGGTGCAAGCTAATGGAGAGAGAAGAGAGGGAGAACAACCTCCCAGAA GACATTCTAGGGAAAATCCGAACCGCGGTGGGCAGTGCCCAACTTCTCATGGCGCAGAAATTCTACCAGT TCAGAGAACTGTGTGAAGAGAACCTGAATCCCAATGCTCACCCGAGACCCACTTCCCAGGACTTGGCGGG GTTTTGGGACATGCTGCAGTTGTCCATAGAGAATATTAGTATGAAATTTGATGAACTTCATCAGCTAAAG GCCAATAATTGGAAACAGATGGATCCTCTTGACAAGAAGGAGCGAAGGGCCCCTCCTCCAGTGCCAAAGA AGCCGGCGAAGGGCCCCGCGCCGCTGATCCGGGAGCGCTCGCTGGAGAGCTCGCAGCGCCAGGAGGCCCG CAAGCGCCTGATGGCCGCCAAGCGCGCCGCGTCGGTCCGCCAGAACTCGGCCACCGAGAGCGCCGAGAGC ATCGAGATCTACATCCCCGAGGCGCAGACCCGGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211400 representing BC062120 Red=Cloning site Green=Tags(s) MKGLSGSRSHHHGITCEAACDSLSHHSDHKPYLLSPVDHHPADHPYYTQRNSFQAECVGPFSDPLASSTF PRRHYTSQQELKDESALVPRTLATKANRLPTNLLDQFERQLPLSRDGYHTLQYKRTAVEHRSDSPGRIRH LVHSVQKLFTKSHSLEGPSKGSVNGGKASPDESQTLRYGKRSKSKERRSESKARSNASNASPTSPSWWSS DDNLDGDMCLYHTPSGVMTMGRCPDRSASQYFMEAYNTISEQAVKASRSNNDIKCSTCANLPVTLDAPLL KKSAWSSTLTVSRAREVYQKASVNMDQAMVKSEACQQERSCQYLQVPQDEWSGYTPRGKDDEIPCRRMRS GSYIKAMGDEDSGDSDTSPKPSPKVAARRESYLKATQPSLTELTTLKISNEHSPKLQIRSHSYLRAVSEV SINRSLDSLDPAGLLTSPKFRSRNESYMRAMSTISQVSEMEVNGQFESVCESVFSELESQAVEALDLPLP GCFRMRSHSYVRAIEKGCSQDDECVSLRSSSPPRTTTTVRTIQSSTGVIKLSSAVEVSSCITTYKKTPPP VPPRTTTKPFISITAQSSTESAQDAYMDGQGQRGDMISQSGLSNSTESLDSMKALTAAIEAANAQIHGPA SQHMGSNAAAVTTTTTIATVTTEDRKKDFKKNRCLSIGIQVDDAEEPEKMAESKTSNKFQSVGVQVEEEK CFRRFTRSNSVTTAVQADLDFHDNLENSLESIEDNSCPGPMARQFSRDASTSTVSIQGSGNHYHACAADD DFDTDFDPSILPPPDPWIDSITEDPLEAVQRSVCHRDGHWFLKLLQAERDRMEGWCKLMEREERENNLPE DILGKIRTAVGSAQLLMAQKFYQFRELCEENLNPNAHPRPTSQDLAGFWDMLQLSIENISMKFDELHQLK ANNWKQMDPLDKKERRAPPPVPKKPAKGPAPLIRERSLESSQRQEARKRLMAAKRAASVRQNSATESAES IEIYIPEAQTRL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC062120
ORF Size	2978 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC062120, AAH62120
RefSeq Size	4714 bp
RefSeq ORF	2978 bp
Locus ID	224997
Gene Summary	Part of the postsynaptic scaffold in neuronal cells.[UniProtKB/Swiss-Prot Function]

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