Pum2 (BC041773) Mouse Tagged ORF Clone

CAT#: MG211386

  • TrueORF®

Pum2 (tGFP-tagged) - Mouse pumilio 2 (Drosophila) (cDNA clone MGC:31021 IMAGE:3986207)



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Mouse Protein / Lysate Request

CNY 7,890.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Pumm2, mKIAA0235
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211386 representing BC041773
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCATGATTTTCAAGCTCTTGCATTAGAATCTCGGGGAATGGGAGAGCTTTTGCCTACCAAAAAGT
TTTGGGAACCTGATGATTCAACAAAAGATGGACAAAAAGGCATATTTCTTGGGGATGATGAATGGAGAGA
GACTGCATGGGGAACTTCTCATCATTCAATGTCCCAGCCTATTATGGTGCAGAGAAGATCTGGACAGAGT
TTTCATGGAAACAGTGAAGTAAATGCAATTCTTTCTCCACGCTCAGAAAGTGGAGGCCTTGGTGTGAGCA
TGGTAGAATATGTATTAAGTTCCTCTCCTGCTGATAAATTGGATTCTCGATTTAGGAAGGGAACTTTTGG
TACTAGAGATGCTGAAACAGATGGACCTGAGAAAGGAGATCAAAAAGGCAAGGCTTCTCCATTTGAGGAG
GACCAAAACAGAGATCTTAAACAAGATGATGAGGACTCTAAAATAAATGGCAGAGGTTTGCCAAATGGAA
TGGATGCCGATTGCAAAGATTTTAATCGCACTCCTGGAAGTCGCCAAGCCTCTCCAACTGAAGTAGTTGA
GCGCCTTGGCCCTAGTACTAATCCCCCAGAAGGATTGGGCCCTCTTCCTAATCCGACAGCGAATAAACCA
CTTGTTGAAGAATTTTCAAACCCTGAAACTCAGAATCTGGATGCAATGGACCAAGTTGGTCTGGATTCCT
TACAGTTTGACTATCCTGGTAATCAGGTACCCATGGATTCTTCAGGAGCTACTGTAGGCCTTTTTGACTA
CAATTCCCAACAGCAGCTCTTTCAGAGGACTAGTGCACTAACAGTTCAGCAGTTAACTGCAGCTCAGCAG
CAGCAGTATGCATTAGCAGCAGCTCAGCAGCCACATATAGCTGGTGTATTCTCAGCAGGCCTTGCTCCAG
CTGCATTTGTGCCAAATCCATATATTATTAGTGCTGCTCCTCCAGGGACTGACCCGTATACTGCAGCAGG
ATTGGCTGCAGCAGCAACATTAGCAGGTCCAGCAGTGGTTCCACCTCAGTATTACGGTGTTCCATGGGGA
GTGTATCCAGCCAATTTATTTCAGCAACAAGCTGCAGCTGCGGCAAGCAACACAGCCAACCAGCAAGCAG
CATCACAAGCTCAGCCTGGACAGCAGCAGGTTCTTCGTCCTGGAGCAGGTCAGCGTCCTATTACTCCAAG
TCAGGGCCAACAAGGGCAGCAAGCAGAGTCACTTGCAGCAGCTGCAAACCCAACTTTGGCTTTTGGTCAG
AGTCTTGCTGCAGGCATGCCAGGCTATCAAGTACTAGCTCCAACTGCCTATTATGATCAGACTGGTGCCT
TAGTGGTTGGCCCCGGAGCAAGAACTGGCCTTGGAGCTCCAGTACGATTAATGGCTCCAACACCTGTCTT
AATAAGTTCAACAGCAGCACAAGCTGCAGCAGCAGCAGCAGCAGCTGGAGGAACTGCAAATAGTCTTACA
GGCAGCACAAATGGTCTGTTTCGGCCAATTGGCACTCAGCCACCACAGCAGCAGCAGCAGCAGCAGCAAC
CAAGCACTAACCTGCAGTCTAATTCATTTTATGGGAGCAGCTCTTTGACTAACAGCTCCCAGAGCAGCTC
TTTATTCTCTCATGGACCTGGCCAACCTGGAAGTGCGTCTCTTGGCTTTGGAAGTGGTAGCTCTTTAGGA
GCTGCTATAGGCTCAGCTCTCAGTGGATTTGGCTCATCAGGAGGACTGACAAACGGTAGTGGTCGGTATA
TCTCTGCAGCACCTGGAGCAGAAGCAAAGTACCGAAGTGCTTCAAGCACTTCCAGTCTATTTAGCTCCAG
CAGCCAGCTCTTTCCTCCTTCTCGGCTCCGCTATAATAGATCTGACATCATGCCCTCCGGCCGCAGTAGG
TTATTGGAAGATTTCAGGAACAACCGCTTCCCCAACCTTCAGCTCAGAGACTTAATTGGACATATAGTCG
AGTTTTCTCAAGACCAGCATGGTTCCAGATTCATACAGCAAAAGCTAGAGAGAGCTACTCCAGCTGAGCG
ACAAATAGTATTTAATGAAATTCTACAGGCAGCCTATCAATTAATGACAGATGTTTTTGGAAACTATGTT
ATACAGAAGTTTTTTGAGTTTGGAAGTTTGGATCAGAAATTAGCCCTGGCTACTCGTATTCGTGGTCATG
TTCTACCATTAGCCTTGCAGATGTATGGCTGCCGTGTTATTCAAAAGGCGTTAGAATCTATTTCTTCTGA
TCAGCAGAGTGAAATGGTTAAGGAACTAGATGGCCATGTACTTAAATGTGTGAAAGATCAAAATGGAAAC
CATGTTGTACAGAAATGCATTGAATGTGTTCAGCCACAGTCACTGCAGTTCATCATCGATGCTTTCAAAG
GACAAGTATTTGTGCTTTCAACCCATCCTTATGGCTGCAGAGTCATTCAGCGTATCTTAGAGCACTGCAC
GGCAGAGCAGACCTTACCCATCTTAGAAGAACTTCACCAACACACAGAACAGTTGGTACAGGATCAGTAT
GGCAATTATGTTATTCAGCATGTACTGGAACATGGTCGACCTGAAGACAAGAGCAAAATTGTTTCCGAAA
TCAGAGGAAAGGTCTTAGCCCTGAGTCAACACAAATTTGCCAGCAATGTAGTAGAAAAGTGTGTTACTCA
TGCCTCCCGTGCTGAGAGAGCTTTACTGATTGATGAGGTCTGCTGTCAGAATGATGGTCCTCACAGTGCC
TTATACACCATGATGAAGGATCAGTATGCCAACTATGTGGTTCAGAAGATGATTGATATGGCTGAGCCTG
CGCAGAGAAAGATAATCATGCACAAGATTCGACCTCATATTACTACTCTTCGCAAATACACATATGGGAA
GCATATACTGGCCAAGTTGGAAAAATACTATCTGAAAAACAGCCCAGATCTAGGGCCAATTGGAGGACCA
CCAAATGGGATGCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211386 representing BC041773
Red=Cloning site Green=Tags(s)

MNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDEWRETAWGTSHHSMSQPIMVQRRSGQS
FHGNSEVNAILSPRSESGGLGVSMVEYVLSSSPADKLDSRFRKGTFGTRDAETDGPEKGDQKGKASPFEE
DQNRDLKQDDEDSKINGRGLPNGMDADCKDFNRTPGSRQASPTEVVERLGPSTNPPEGLGPLPNPTANKP
LVEEFSNPETQNLDAMDQVGLDSLQFDYPGNQVPMDSSGATVGLFDYNSQQQLFQRTSALTVQQLTAAQQ
QQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAAPPGTDPYTAAGLAAAATLAGPAVVPPQYYGVPWG
VYPANLFQQQAAAAASNTANQQAASQAQPGQQQVLRPGAGQRPITPSQGQQGQQAESLAAAANPTLAFGQ
SLAAGMPGYQVLAPTAYYDQTGALVVGPGARTGLGAPVRLMAPTPVLISSTAAQAAAAAAAAGGTANSLT
GSTNGLFRPIGTQPPQQQQQQQQPSTNLQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSASLGFGSGSSLG
AAIGSALSGFGSSGGLTNGSGRYISAAPGAEAKYRSASSTSSLFSSSSQLFPPSRLRYNRSDIMPSGRSR
LLEDFRNNRFPNLQLRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQIVFNEILQAAYQLMTDVFGNYV
IQKFFEFGSLDQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSEMVKELDGHVLKCVKDQNGN
HVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQDQY
GNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVCCQNDGPHSA
LYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEKYYLKNSPDLGPIGGP
PNGML

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC041773
ORF Size 2957 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC041773, AAH41773
RefSeq Size 3295 bp
RefSeq ORF 2957 bp
Locus ID 80913
Gene Summary Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3' UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3' UTR and facilitating miRNA regulation. Plays a role in cytoplasmic sensing of viral infection. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm. May regulate DCUN1D3 mRNA levels. May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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