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Ipo11 (BC031900) Mouse Tagged ORF Clone
CAT＃: MG211384
- TrueORF®
Ipo11 (tGFP-tagged) - Mouse importin 11 (cDNA clone MGC:39010 IMAGE:5364208)
ORF Plasmid: DDK
"BC031900" in other vectors (4)
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CNY 10,740.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1700081H05Rik, 2510001A17Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211384 representing BC031900
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTCAATAGTGCCAGCTCTGTGGTTCTTCAGGTCCTAACACAGGCCACCAGTCAGGATACTGCTG TGTTGAAGCCAGCCGAGGAGCAGCTGAAACAGTGGGAGACACAGCCAGGTTTCTATTCAGTGTTGCTGAA TATTTTTACGAATCACACCCTTGATATAAATGTACGGTGGCTTGCTGTGCTGTATTTTAAACATGGGATT GATCGTTACTGGAGGCGTGTCGCACCGCATGCTCTCTCAGAAGAGGAAAAGTCAACATTGCGTGCAGGGC TCATCACCAACTTTAATGAACCAATAAACCAGATTGCGACCCAGATCGCAGTGCTGATTGCCAAGGTTGC TCGGTTAGACTGTCCTAGGCAGTGGCCGGAGCTCATTCCCACTCTTGTAGAGTCTGTCAAAGTCCAGGAT GACCTTCGCCAGCACAGAGCTCTGCTGACCTTCTACCATGTCACCAAGACGCTGGCATCCAAACGCCTGG CCGCTGACAGAAAGCTATTCTATGATTTAGCTTCTGGAATTTATAATTTTGCCTGTTCTCTGTGGAACCA TCATACAGACACATTTCTGCAGCACGTTTCTTCTGGCAATGAAGCTGCGGTTCTGAGTTCACTAGAGCGA ACACTGCTATCGTTAAAAGTGCTGCGGAAGTTAACTGTGAATGGATTTGTGGAACCTCATAAGAATATGG AGGTGATGGGTTTTTTACATGGAATATTTGAACGTCTAAAACAGTTTCTGGAGTGCAGCAGAAGTATAGG TACAGATAATGTGTGTCGAGATAGACTGGAAAAGACCATCATTCTTTTTACTAAAGTGCTCTTGGACTTC TTGGATCAACACCCCATTTCATTTACTCCTCTAATTCAGAGGTCACTGGAATTTTCTGTAAGCTATGTTT TTACTGAAGTTGGTGAAGGCGTTACATTTGAGCGATTCATTGTCCAGTGCATGAATCTTATTAAGATGAT TGTCAAAAATTATGCTTATAAGCCATCCAAAAATTTTGAAGATAGCAGCCCTGAAACACTTGAAGCCCAT AAGATTAAGATGGCATTCTTCACATATCCCACGTTGACAGAGATATGTCGACGATTAGTCTCTCATTATT TTTTATTAACGGAAGAAGAACTGACCATGTGGGAGGAAGATCCAGAAGGCTTTACAGTGGAAGAAACAGG AGGAGACTCTTGGAAATACAGTTTGAGGCCTTGCACTGAAGTATTATTTATAGATATATTCCACGAATAT AATCAAACTCTTACTCCTGTACTTCTAGAAATGATGCAAACTCTTGAAGGTCCAACCAATGTAGAAGATA TGAATGCACTGTTAATCAAAGATGCTGTGTATAATGCTGTGGGATTAGCTGCTTTTGAGCTGTTTGACAG TGTTGATTTTGATCAGTGGTTTAAAACCCAACTTCTTCCCGAATTACAAGTTTCTCATAATAGGTATAAG CCATTACGACGCAGGGTGATTTGGCTCATTGGTCAGTGGATTTCTGTGAAATTCAAGTCTGACTTGAGAC CCATGCTGTATGAAGCAATCTGTAACTTGCTTCAAGATCAGGATTTAGTGGTCCGAATTGAAACAGCAAC AACTCTGAAGTTAACTGTTGATGATTTTGAATTTAGGACAGATCAGTTTTTACCGTATCTGGAAACCATG TTCACACTGCTTTTCCAGTTGCTGCAGCAGGTTACAGAGTGTGATACAAAGATGCACGTGCTGCACGTCC TCTCCTGTGTGATCGAAAGAGTCAACGTGCAGATCCGACCATATGTGGGGTGTTTGGTACAGTATTTGCC TCTTCTCTGGAAGCAGAGCGAAGAACACAACATGCTGCGATGCGCTATTCTGACAACACTTATCCACCTT GTTCAGGGATTAGGAGCAGACAGCAAGAACCTGTACCCTTTCCTGCTCCCAGTTATCCAGCTGAGTACAG ATGTGTCTCAGCCTCCACATGTCTATCTTCTGGAAGATGGCCTGGAGTTATGGTTAGTGACATTGGAGAA CAGCCCATGTGTCACACCAGAGCTGCTTCGTATATTTCAGAACATGTCACCTCTTCTTGAGCTTCCATCC AGCTGCCTTGCAAACGAATTAAGTTCAGAGAATCTCAGAACCTGCTTTAAGATCATCAATGGCTATATCT TTTTATCATCAACAGAATTTTTACAGACATATGCAGCAGGTTTGTGCCAGTCCTTTTATGAGCTGCTACC AGAGATTACTACAGAAGGTCAAGTTCAAGTGCTGAAGGTTGTGGAAAATGCCCTTAAAGTGAATCCAGTA TTAGGCCCACAGATGTTTCAGCGAATTCTACCCTGTGTTTTCAGAGGTGTTATTGAAGGCGAGAGGTATC CAGTAGTGATGTCCATTTATCTTGCTGTCATGGGTCGAGTCTTACTCCAGAATACCAGCTTCTTTTCTTC ACTTCTTAATGAGATGGGACACGAGTTTAATCAGGAGATGGACCAGCTTTTAGGAAATGTGATTGAAATG TGGGTCGATCGAATGGACAACATCACACAGCCCGAAAGAAAGAAACTCTCAGCTTTGGCTTTGCTTTCTC TTCTGCCTTCTGATAATAGTGTTATCCAAGATAAATTTTGTGGAATTATAAATATCTCAGTGGAGGCTCT TCATGATGTCATGACAGAAGACCCTGAAACAAGAACTTACAAAGATTGTATGTTGATGTCTCAACACGAG GAACCGAAAGTAACAGAAGATGAAGAGCCACCCACAGAACAAGACAAGAGGAAAAAGATGCTGGCCCTGA AGGACCCGGTGCACACAGTGTCTCTGCAGCAGTTCATCTATGAGAAGCTCAAGGCGCAGCAGGAGATCCT GGGGGAGCAAGGCTTCCAGTCCCTCATGGAAACGGTGGACACAGAGATTGTCACCCAGCTGCAGGAGTTT TTGCAAGGATTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211384 representing BC031900
Red=Cloning site Green=Tags(s) MDLNSASSVVLQVLTQATSQDTAVLKPAEEQLKQWETQPGFYSVLLNIFTNHTLDINVRWLAVLYFKHGI DRYWRRVAPHALSEEEKSTLRAGLITNFNEPINQIATQIAVLIAKVARLDCPRQWPELIPTLVESVKVQD DLRQHRALLTFYHVTKTLASKRLAADRKLFYDLASGIYNFACSLWNHHTDTFLQHVSSGNEAAVLSSLER TLLSLKVLRKLTVNGFVEPHKNMEVMGFLHGIFERLKQFLECSRSIGTDNVCRDRLEKTIILFTKVLLDF LDQHPISFTPLIQRSLEFSVSYVFTEVGEGVTFERFIVQCMNLIKMIVKNYAYKPSKNFEDSSPETLEAH KIKMAFFTYPTLTEICRRLVSHYFLLTEEELTMWEEDPEGFTVEETGGDSWKYSLRPCTEVLFIDIFHEY NQTLTPVLLEMMQTLEGPTNVEDMNALLIKDAVYNAVGLAAFELFDSVDFDQWFKTQLLPELQVSHNRYK PLRRRVIWLIGQWISVKFKSDLRPMLYEAICNLLQDQDLVVRIETATTLKLTVDDFEFRTDQFLPYLETM FTLLFQLLQQVTECDTKMHVLHVLSCVIERVNVQIRPYVGCLVQYLPLLWKQSEEHNMLRCAILTTLIHL VQGLGADSKNLYPFLLPVIQLSTDVSQPPHVYLLEDGLELWLVTLENSPCVTPELLRIFQNMSPLLELPS SCLANELSSENLRTCFKIINGYIFLSSTEFLQTYAAGLCQSFYELLPEITTEGQVQVLKVVENALKVNPV LGPQMFQRILPCVFRGVIEGERYPVVMSIYLAVMGRVLLQNTSFFSSLLNEMGHEFNQEMDQLLGNVIEM WVDRMDNITQPERKKLSALALLSLLPSDNSVIQDKFCGIINISVEALHDVMTEDPETRTYKDCMLMSQHE EPKVTEDEEPPTEQDKRKKMLALKDPVHTVSLQQFIYEKLKAQQEILGEQGFQSLMETVDTEIVTQLQEF LQGF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC031900 |
| ORF Size | 2954 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031900, AAH31900 |
| RefSeq Size | 4259 bp |
| RefSeq ORF | 2954 bp |
| Locus ID | 76582 |
| Gene Summary | Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPL12, and of UBE2E3 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220849 | Ipo11 (untagged) - Mouse importin 11 (cDNA clone MGC:39010 IMAGE:5364208), (10ug) |
CNY 9,120.00 |
|
| MR211384 | Ipo11 (Myc-DDK-tagged) - Mouse importin 11 (cDNA clone MGC:39010 IMAGE:5364208) |
CNY 9,790.00 |
|
| MR211384L3 | Lenti ORF clone of Ipo11 (Myc-DDK-tagged) - Mouse importin 11 (cDNA clone MGC:39010 IMAGE:5364208) |
CNY 11,690.00 |
|
| MR211384L4 | Lenti ORF clone of Ipo11 (mGFP-tagged) - Mouse importin 11 (cDNA clone MGC:39010 IMAGE:5364208) |
CNY 11,690.00 |
