Ap2a1 (BC031433) Mouse Tagged ORF Clone

CAT#: MG211368

  • TrueORF®

Ap2a1 (tGFP-tagged) - Mouse adaptor protein complex AP-2, alpha 1 subunit (cDNA clone MGC:25419 IMAGE:4481643)



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Mouse Protein / Lysate Request

CNY 7,890.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Adtaa
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211368 representing BC031433
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGCTGTATCCAAAGGCGATGGCATGCGTGGGCTCGCCGTGTTCATCTCCGACATCCGGAACTGCA
AGAGCAAAGAGGCTGAGATCAAGAGGATCAACAAGGAACTGGCCAACATCCGTTCCAAGTTCAAAGGGGA
CAAGGCCTTGGATGGCTACAGTAAAAAGAAGTATGTGTGTAAGCTGCTCTTCATATTCCTGCTTGGCCAT
GACATTGACTTTGGACATATGGAGGCCGTGAACCTGCTAAGCTCTAACAAGTACACGGAGAAGCAGATAG
GGTACCTGTTCATCTCAGTCCTGGTGAACTCGAACTCAGAACTGATCCGGCTCATCAACAACGCTATCAA
GAATGACCTGGCCAGTCGCAACCCCACCTTCATGTGCCTGGCCTTGCACTGTATCGCTAACGTGGGCAGC
CGTGAGATGGGCGAGGCTTTTGCTGCAGACATTCCCCGAATCCTGGTGGCTGGGGACAGCATGGACAGTG
TGAAGCAGAGTGCCGCCCTATGCCTACTGCGACTCTACAAGGCCTCGCCCGACTTGGTGCCCATGGGCGA
GTGGACGGCACGTGTAGTGCACTTGCTCAATGATCAGCACATGGGAGTGGTCACAGCTGCTGTCAGCCTC
ATCACCTGTCTCTGCAAGAAGAATCCGGATGACTTCAAGACCTGTATCTCCCTGGCTGTGTCTCGCTTAA
GCCGGATCGTCTCCTCAGCCTCCACTGACCTCCAGGACTACACTTACTACTTCGTTCCTGCACCCTGGCT
CTCTGTGAAGCTACTGCGGCTGCTCCAGTGTTACCCACCACCAGAGGATGCAGCCGTGAAAGGGCGGTTA
GTGGAGTGTCTGGAGACTGTGCTCAACAAGGCCCAGGAGCCTCCCAAGTCCAAGAAGGTGCAGCACTCCA
ACGCCAAGAACGCTATCCTCTTTGAGACCATTAGCCTCATCATCCACTATGACAGTGAGCCCAACCTCCT
GGTCCGCGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCACCGGGAGACTAACCTGCGCTACCTGGCCCTG
GAGAGCATGTGCACGCTGGCCAGCTCCGAGTTCTCCCACGAGGCCGTCAAGACCCACATTGATACAGTTA
TTAATGCCCTCAAGACGGAGCGGGACGTCAGTGTGAGGCAGCGGGCGGCTGATCTCCTGTATGCCATGTG
TGACCGGAGCAATGCCAAGCAGATTGTGTCAGAGATGCTGCGGTACCTGGAGACTGCTGACTATGCCATC
CGAGAGGAGATCGTGCTGAAGGTGGCCATCCTGGCTGAGAAGTATGCAGTGGACTACAGCTGGTACGTGG
ACACCATCCTCAACCTCATCCGCATCGCGGGCGACTATGTGAGCGAGGAGGTGTGGTACCGCGTGCTGCA
GATCGTCACCAACCGTGATGACGTCCAGGGTTATGCTGCCAAGACAGTGTTTGAGGCCCTCCAGGCCCCA
GCCTGTCATGAGAACATGGTGAAGGTCGGTGGCTACATCCTTGGGGAGTTTGGGAACTTGATTGCTGGGG
ACCCACGCTCCAGCCCACCAGTGCAGTTCTCGCTGCTGCACTCCAAGTTCCACCTGTGCAGCGTGGCCAC
CCGCGCTCTGTTGCTGTCCACCTACATCAAGTTCATCAACCTCTTCCCTGAGACCAAGGCCACCATCCAA
GGGGTTCTGCGTGCCGGCTCCCAGCTGCGAAATGCCGACGTGGAGCTACAGCAGCGGGCCGTGGAGTACC
TCACCCTCAGCTCCGTAGCCAGCACCGATGTTCTGGCTACGGTGCTAGAAGAAATGCCCCCATTTCCCGA
GCGGGAGTCGTCCATCTTGGCCAAGCTGAAGCGCAAGAAGGGCCCTGGGGCAGCCAGTGCCCTAGATGAC
AGCCGCAGGGACACCAGCAGCAATGACATCAATGGGGGTGTGGAGCCCACCCCCAGCACTGTGTCGACCC
CCTCACCCTCCGCGGACCTCTTAGGGCTGCGGGCAGCCCCTCCCCCTGCTGCACCCCCGGCTCCCGTAGG
CGGGAACCTCCTGGTGGATGTCTTCTCTGACGGCCCCACTGCACAGCCCAGCCTGGGGCCCACTCCTGAG
GAGGCCTTCCTCAGCGAGCTGGAGCCCCCTGCCCCTGAGAGCCCCATGGCTTTGTTGGCTGACCCAGCTC
CAGCTGCTGACCCAGGTCCTGAGGACATAGGCCCTCCCATTCCAGAAGCAGATGAACTGTTGAATAAGTT
CGTGTGTAAGAATAGTGGGGTCTTGTTTGAGAACCAGCTGCTGCAGATTGGAGTCAAGTCTGAGTTCCGG
CAGAACCTGGGCCGCATGTATCTCTTCTATGGCAACAAGACTTCTGTGCAGTTCCAGAACTTCTTGCCCA
CCGTGGTCCATCCTGGGGACCTCCAGACTCAGCTGGCGGTGCAGACCAAGCGTGTGGCGGCACAAGTGGA
CGGTGGCGCACAGGTGCAGCAAGTACTCAACATTGAGTGTCTGCGAGACTTCCTGACGCCGCCACTGTTG
TCGGTGCGCTTCCGGTACGGTGGCACCGCCCAGTCCCTCACTCTGAAGCTCCCAGTGACCATCAACAAAT
TCTTCCAGCCCACAGAGATGGCGGCCCAAGACTTTTTCCAGCGCTGGAAGCAGCTGAGCCTCCCCCTGCA
GGAGGCACAGAAAATCTTCAAAGCCAACCACCCGATGGATGCTGAAGTTACTAAGGCCAAGCTTCTGGGG
TTTGGCTCTGCTCTTCTGGACAATGTGGATCCCAACCCTGAGAACTTTGTGGGTGCTGGAATCATCCAGA
CGAAGGCCCTGCAGGTGGGGTGTCTGCTTCGGCTGGAGCCCAATGCCCAGGCCCAAATGTACCGTCTAAC
CCTGCGCACCAGCAAAGAGCCTGTCTCCCGTCACTTGTGTGAGCTGCTGGCCCAGCAGTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211368 representing BC031433
Red=Cloning site Green=Tags(s)

MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGH
DIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGS
REMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSL
ITCLCKKNPDDFKTCISLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRL
VECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLAL
ESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI
REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAP
ACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQ
GVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDD
SRRDTSSNDINGGVEPTPSTVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPE
EAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFR
QNLGRMYLFYGNKTSVQFQNFLPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLL
SVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLG
FGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC031433
ORF Size 2933 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC031433, AAH31433
RefSeq Size 3407 bp
RefSeq ORF 2933 bp
Locus ID 11771
Gene Summary Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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