Wdr59 (BC066082) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5430401O09Rik; Cdw12
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211357 representing BC066082 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCGCTGGAGCAGCGAGAACGTGGTGGTGGAGTTTCGAGACTCTCAGGCAACTGCCATGTCTG TGGACTGTCTTGGGCAGCATGCAGTGCTTTCTGGCCGACGATTCCTGTACATCGTCAATCTAGATGCCCC TTTTGAAGGTCACCGGAAGATCTCCCGCCAGAGCAAATGGGACATTGGAGCTGTGCAGTGGAACCCTCAT GACAGCTTCGCACACTATTTTGCAGCTTCGAGTAATCAACGGGTCGACCTTTATAAGTGGAAAGATGGCA GCGGGGAAGTTGGTACAACTTTACAGGGGCACACTCGAGTTATCAGCGACTTGGACTGGGCAGTGTTTGA GCCAGATCTCCTGGTCACCAGCTCTGTGGACACCTACATCTACATCTGGGATATCAAAGACACAAGGAAA CCTACTGTTGCACTGTCGGCTGTAGCTGGTGCCTCCCAGGTCAAATGGAATAAGAAGAACGCCAACTACC TTGCCACCAGTCACGATGGTGACGTACGGATATGGGACAAGCGAAAACCCAGTACAGCTGTGGAATATCT AGCAGCCCACCTCTCCAAGATCCACGGCCTGGACTGGCACCCAGACAGTGAGCACATCTTTGCCACCTCC AGTCAAGATAACTCTGTCAAGTTCTGGGATTACCGCCAGCCTCGGAAATACCTCAATATCCTCCCTTGCC AGGTGCCTGTCTGGAAAGCCAGATACACGCCTTTCAGCAACGGGCTAGTGACCGTGATGGTTCCCCAGCT GCGGAGGGAGAACAGCCTGCTCCTGTGGAACGCGTCTGACTTGAACGCTCCAGTTCACACCTTTGTGGGT CATGATGACGTGGTGCTGGAGTTCCAGTGGAGGAGGCAGAAGGAAGGATCCAAGGACTATCAACTGGTTA CATGGTCCCGTGATCAGACCTTGAGAATGTGGCGAGTGGACTATCAAATGCAGAGGCTCTGTGCCAATGA CATACTGGATGGTGTTGATGAGTTCATTGAGAGCATCTCTCTCCTGCCGGAACCAGAGAAGACCCCACAT CCTCAAGACATAGATCACCAGCCCAGCTTAAGCCATGGAGAGGAAGATGCCATAAAGGAAGACCCTCCCA GCAGTTTGCTGGAAGAGAAGAGATCAGACCAACTAGGCCTCCCCCAGACTCTGCAACAGGAGTTCTCCTT GATCAACGTGCAGATCCGGAATGTCAATGTGGAGATGGACGCTGCAGACCGGAGCTGCACAGTGTCTGTG CACTGCAGCAACCACCGTGTGAAGATGCTGGTGACCTTCCCGGCGCAGTACCCTAACAACGCTGCTCCTT CCTTCCAGTTCATTAATCCTACCACTATCACGTCTGCCGTGAAGGCCAAGCTGCTGAAGATCCTAAAGGA CACTTCACTGCAGAAAGTGAAACGCAACCAGAGCTGTTTGGAGCCTTGCCTGCGCCAGCTTGTCTCGTGC CTTGAGTCTTTTGTGAACCAAGAGGACAGTGCTTCTAGCAACCCATTTGCACTCCAAAACTCTGTCACAC CACCCTTGCCGACATTTGCACGGGTGACCACTGCCTACGGCTCCTACCAGGATGCCAACATTCCCTTTCC TAGGACCTCTGGGGCCAGGTTCTGTGGAGCAGGTTACCTGGTGTACTTCACACGACCCATGACGATGCAT CGAGCAGTGTCTCCCACAGAGCCTACTCCTAGGTCTCTCTCAGCCCTGTCTGCTTATCATACTGGTTTGA TTGCACCAATGAAGATCCGCACCGAGGCCCCTGGGAACCTGCGTCTGTACAGTGGGAGCCCAACTCGCAG TGAGAAAGAGCAGGTCTCCATCAGTTCCTTCTATTACAAGGAACGGAAATCGAGGAGGTGGAAGAGTAAG CGGGAAGGCTCTGACTCCGGCAACCGACCCATTAAGGCTGCTGGCAAGGTCATCATCCAGGATGTCTCGT GCCTCCTTCCCGTTCATAAGTCCTTGGGAGAGCTGTACATATTGAATGTGAACGACACTCAGGAGACATG TCAGAAGAATGCCACTTCTGCCATGCTTGTGGGAAGGAAGGATCTTGTCCAGGTTTGGTCGCTGGCTACG GTAGCTACAGATCTTTGCCTTGGGCCGAAGTCTGACCCAGATTTGGAAACACCTTGGGCTCGACATCCAT TTGGGCGACAACTGCTGGAGTCCCTACTGGCTCACTATTGCCAACTCCGAGATGTTCAGACCTTGGCTAT GCTCTGTAGCGTGTTTGAAGCCCAGTCCCGGCCTCAGGGCTTACCAAACCCTTTTGGGCCTTTCCCTAAT CGTTCTTCTAATCTTGTGGTGTCCCACAGCCGCTATCCCAGCTTCACCTCCTCGGGGTCCTGCTCCAGCA TGTCAGATCCAGGGTTCAACACCGGTGGCTGGAACATAGCAGGAAGAGAGACAGAACACATATCTTCACC TTGGGGGGAGTCTTCGCCAGAGGAGCTCCGCTTTGGGAGTCTGACCTACAGTGACCCCCGGGAGCGGGAA CGAGACCAGCATGATAAAAACAAAAGGCTCCTGGACCCTGCCAATACCCAGCAGTTTGACGACTTTAAGA AGTGCTACGGAGAGATCCTCTACCGCTGGGGTCTGAGAGAGAAGCGAGCAGAAGTGCTGAAGTTTGTGTC CTGCCCTCCTGACCCTCACAAAGGGATCGAGTTTGGTGTGTACTGCAGTCACTGTCGGAGCGAGGTCCGT GGCACCCAGTGCGCCATCTGCAAAGGCTTCACCTTCCAGTGTGCCATCTGCCATGTGGCCGTGCGGGGTT CATCCAACTTCTGCCTGACCTGTGGGCATGGTGGCCACACGAGCCACATGATGGAGTGGTTTCGGACCCA GGAGGTATGTCCCACGGGGTGTGGCTGCCACTGCCTGCTGGAGAGCACCTTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211357 representing BC066082 Red=Cloning site Green=Tags(s) MAARWSSENVVVEFRDSQATAMSVDCLGQHAVLSGRRFLYIVNLDAPFEGHRKISRQSKWDIGAVQWNPH DSFAHYFAASSNQRVDLYKWKDGSGEVGTTLQGHTRVISDLDWAVFEPDLLVTSSVDTYIYIWDIKDTRK PTVALSAVAGASQVKWNKKNANYLATSHDGDVRIWDKRKPSTAVEYLAAHLSKIHGLDWHPDSEHIFATS SQDNSVKFWDYRQPRKYLNILPCQVPVWKARYTPFSNGLVTVMVPQLRRENSLLLWNASDLNAPVHTFVG HDDVVLEFQWRRQKEGSKDYQLVTWSRDQTLRMWRVDYQMQRLCANDILDGVDEFIESISLLPEPEKTPH PQDIDHQPSLSHGEEDAIKEDPPSSLLEEKRSDQLGLPQTLQQEFSLINVQIRNVNVEMDAADRSCTVSV HCSNHRVKMLVTFPAQYPNNAAPSFQFINPTTITSAVKAKLLKILKDTSLQKVKRNQSCLEPCLRQLVSC LESFVNQEDSASSNPFALQNSVTPPLPTFARVTTAYGSYQDANIPFPRTSGARFCGAGYLVYFTRPMTMH RAVSPTEPTPRSLSALSAYHTGLIAPMKIRTEAPGNLRLYSGSPTRSEKEQVSISSFYYKERKSRRWKSK REGSDSGNRPIKAAGKVIIQDVSCLLPVHKSLGELYILNVNDTQETCQKNATSAMLVGRKDLVQVWSLAT VATDLCLGPKSDPDLETPWARHPFGRQLLESLLAHYCQLRDVQTLAMLCSVFEAQSRPQGLPNPFGPFPN RSSNLVVSHSRYPSFTSSGSCSSMSDPGFNTGGWNIAGRETEHISSPWGESSPEELRFGSLTYSDPRERE RDQHDKNKRLLDPANTQQFDDFKKCYGEILYRWGLREKRAEVLKFVSCPPDPHKGIEFGVYCSHCRSEVR GTQCAICKGFTFQCAICHVAVRGSSNFCLTCGHGGHTSHMMEWFRTQEVCPTGCGCHCLLESTF SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC066082
ORF Size	2924 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC066082, AAH66082
RefSeq Size	5137 bp
RefSeq ORF	2924 bp
Locus ID	319481
Gene Summary	As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. It is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.