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Vps50 (NM_024260) Mouse Tagged ORF Clone
CAT＃: MG211331
- TrueORF®
Ccdc132 (tGFP-tagged) - Mouse coiled-coil domain containing 132 (Ccdc132)
ORF Plasmid: DDK
"NM_024260" in other vectors (3)
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CNY 10,550.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1700034M03Rik; 8430415E05Rik; BLM; C87205; Ccdc132; mKIAA1861 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211331 representing NM_024260
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAAAAATCAAATCTCTCATGACCCGGCAGGGTCTGAAGAGCCCTCCGGAGAGCCTCAATGATCTTG GTGCCTTTGAGAGCCTTCGGGTCCCTGGAAAGGAGGAATTCAGGGAGCTTCGAGAACAGCCAAGCGACCC TCAAGCTGAGCAAGAGTTGATTAATAGCATTGAACAAGTCTATTTTTCTGCGGATCCATTTGATATTGTT AAATATGAGCTGGAGAAGCTTCCTCCTGTTCTCAATCTGCAAGAGTTAGAGGAGTACAGAGACAAATTAA AGCAACAACAATCTGCAGTCTCTAAAAAAGTGGCAGATTTGATTCTTGAAAAGCAGCCTGCTTATGTAAA GGAACTTGAAAGAGTCACATCACTGCAGACAGGTCTTCAGTTGGCTGCTGTCATCTGCACAAATGGGAGG AGGCACTTGAATATTGCAAAGGAAGGTTTTACTCAGGCTAGCTTAGGCCTTCTTGCAAATCAAAGGAAGC GTCAGTTGCTAATTGGACTTCTGAAGTCGCTGAGAACCATAAAAACATTGCAAAGAACTGACATACGCTT AAGTGAAATGCTGGAGGAAGAGGACTATCCAGGAGCTATTCAGCTGTGCCTAGAATGTCAGAAGGCTGCC AGCACTTTTAAACACTACAGCTGTATAAGTGAGCTGAATTCAAAGCTGCAAGATACTTTGGAGCAAATTG AGGAACAGTTGGATGTAGCTCTTTCCAAAATCTGCAAGAATTTTGACATTAACCATTATACCAAGGTTCA GCAAGCTTACCGGCTTCTTGGAAAAACACAGACTGCGATGGATCAGCTTCATATGCACTTCACTCAAGCC ATTCACAACACCGTATTTCAGGTTGTGCTTGGCTATGTGGAGCTGTGTGCAGGAAACACGGATACAAAAT TCCAGAAGCTTCAGTATAAAGATCTCTGTACGCATGTGACACCAGACAGCTATATTCCATGCCTGGCAGA CCTGTGCAAAGCACTGTGGGAAGTCATGCTCAGCTACTACAGAACCATGGAGTGGCACGAGAAACATGAC AATGAGGAAACAGCTGCAGCTGCTGAAGGAAGCAATGTGATGAGTACTGAGGAGGCCACTTTTGACCGTG GCTATGTAAAGAAGAAGTTAGAGCACGGCCTTACCCGGATATGGCAGGATGTTCAACTAAAAGTAAAAAC CTACTTGCTTGGAACTGATTTATCTATATTCAAATATGATGATTTCATCTTTGTTCTGGATATTGTCAGC AGGTTGATGCAAGTTGGAGAAGAATTTTGTGGTAGCAAGTCTGAAGTTTTGCAGGAGTCCATTAGAAAAC AAAGTGTCAATTACTTCAAGAACCACCATAGGATACGACTTGATGAACTGAGAATGTTCTTAGAGAATGA GACATGGGAGCTTTGCCCTGTTAAGTCAAATTTTAGCATCTTGCAGCTTCATGAGTTTAAATTCCTGGAA CAGTCTCGCTCCCCATCAGTTTCACCTAGTAAGCAGCCATCAGCAACTTCTTCAAAGCCTGTGACTTTGT TTGAGCAGTACTGCAGTGGTGGGAATCCATTTGAAATACAAGCCGACCACAAAGATGAAGAAACTGAAGA TGTCTTAGCCTCTAATGGGTATGAGTCTGATGAGCAAGAGAAGAGCGCCTATCAGGACTATGACAGTGAC AGCGACGTCCCTGAGGAGCTGAAGCGGGATTACGTGGACGAGCAGACCGGCGACGTGCCCGTGAAGAGTG TTTCCCGAGAGACCCTGAAGAGCAGGAAGAAGTCGGATTACAGTCTAAACAAAGTGAATGCACCCATCTT AACAAACACAACACTGAATGTAATAAGGCTTGTTGGAAAGTACATGCAGATGATGAACATCCTTAAGCCA ATCGCCTTCGATGTGATCCATTTCATGTCTCAGCTCTTTGACTATTACTTGTATGCCATATATACCTTTT TTGGTCGGAATGATTCATTGGAATCAACAGGACTTGGCCTTAGTAGTAGCAGACTAAAAACAACTCTAAA CAGAATTCAAGAAAGCCTTATTGACCTGGAGGGCTCAGCTGATCCAACTGCCACTCTCACAGCAGCCGAG GAAAGAAAGGAGAAGGTACCCAGCCCACACCTCAATCAGCTAGTGATTTTGACATCTGGGGATACATTGT ATGGGTTGGCAGAGAGAGTGGTAGCCACAGAATCCTTAGTGTTCTTGGCTGAGCAGTTTGAGTTCCTTCA GCCACATTTGGATGCTGTGATGCCTGCAGTCAAAAAGCCCTTCCTGCAGCAGTTTTACTCCCAGACAGTC TCAACTGCCAGTGAACTGCGCAAGCCCATTTACTGGATTGTGGCTGGTAAAGCTATTGATTATGAACAGA TGCTTCTCCTAATGATGAATGTGAAGTGGGATGTCAAAGAGATCATGTCACAGCACAACATCTATGTAGA TGCATTGCTGAAGGAATTTGAGCAGTTTAACAAGCGGTTAAATGAAGTTTCTAAGAGAGTTCGGATACCT CTGCCTGTATCAAATATACTTTGGGAGCATTGTATACGATTAGCTAACAGAACTATTGTGGAAGGATACG CCAATGTCAAGAAATGCAGTAATGAGGGGCGTGCCTTGATGCAATTGGATTTTCAACAGTTTTTAATGAA ACTCGAAAAACTAACTGACATTAGACCTATTCCTGATAAAGAATTTGTGGAAACGTATATCAAAGCCTAC TACCTAACTGAGAATGACATGGAGCGTTGGATCAAAGAGCACAGGGAATACTCAACGAAGCAGTTGACCA ACCTGGTGAATGTTTGCCTAGGATCTCATATCAATAAGAAAGCAAGACAGAAGCTCCTAGCAGCTATAGA TGAGATAGACAGGCCTAAAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211331 representing NM_024260
Red=Cloning site Green=Tags(s) MQKIKSLMTRQGLKSPPESLNDLGAFESLRVPGKEEFRELREQPSDPQAEQELINSIEQVYFSADPFDIV KYELEKLPPVLNLQELEEYRDKLKQQQSAVSKKVADLILEKQPAYVKELERVTSLQTGLQLAAVICTNGR RHLNIAKEGFTQASLGLLANQRKRQLLIGLLKSLRTIKTLQRTDIRLSEMLEEEDYPGAIQLCLECQKAA STFKHYSCISELNSKLQDTLEQIEEQLDVALSKICKNFDINHYTKVQQAYRLLGKTQTAMDQLHMHFTQA IHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHVTPDSYIPCLADLCKALWEVMLSYYRTMEWHEKHD NEETAAAAEGSNVMSTEEATFDRGYVKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIVS RLMQVGEEFCGSKSEVLQESIRKQSVNYFKNHHRIRLDELRMFLENETWELCPVKSNFSILQLHEFKFLE QSRSPSVSPSKQPSATSSKPVTLFEQYCSGGNPFEIQADHKDEETEDVLASNGYESDEQEKSAYQDYDSD SDVPEELKRDYVDEQTGDVPVKSVSRETLKSRKKSDYSLNKVNAPILTNTTLNVIRLVGKYMQMMNILKP IAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSRLKTTLNRIQESLIDLEGSADPTATLTAAE ERKEKVPSPHLNQLVILTSGDTLYGLAERVVATESLVFLAEQFEFLQPHLDAVMPAVKKPFLQQFYSQTV STASELRKPIYWIVAGKAIDYEQMLLLMMNVKWDVKEIMSQHNIYVDALLKEFEQFNKRLNEVSKRVRIP LPVSNILWEHCIRLANRTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAY YLTENDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQKLLAAIDEIDRPKR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024260 |
| ORF Size | 2892 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024260.5, NP_077222.4 |
| RefSeq Size | 3780 bp |
| RefSeq ORF | 2895 bp |
| Locus ID | 73288 |
| UniProt ID | Q8CI71 |
| Gene Summary | Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane. Within the EARP complex, required to tether the complex to recycling endosomes. Not involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR211331 | Ccdc132 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 132 (Ccdc132), transcript variant 1 |
CNY 9,600.00 |
|
| MR211331L3 | Lenti ORF clone of Ccdc132 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 132 (Ccdc132), transcript variant 1 |
CNY 11,500.00 |
|
| MR211331L4 | Lenti ORF clone of Ccdc132 (mGFP-tagged) - Mouse coiled-coil domain containing 132 (Ccdc132), transcript variant 1 |
CNY 11,500.00 |
