Ppp4r1 (BC029761) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Pp4r1, MGC31567
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211287 representing BC029761 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCTCTCCCTGCTCCAGGAGGACCTGCCGGAGGACGCGGACGGACTTGATGTGGATGACTACA GCTCAGAGTCTGATGTGATTATTATACCTTCAGCCCTGGACTTCGTCTCACAAGATGAAATGTTGACACC CTTGGGGAGGCTGGACAAGTATGCTGCAAGTGAGAACGTATTTAACAGACAAATGGTGGCCCGGAGTTTG CTGGATACTCTGAGGGAAGTCTGCGATGATGAGAGAGACTGCATTGCTGTCTTGGAAAGAATTAGCCGAT TAGCTGATGACTCCGAACCAACTGTGAGAGCGGAGCTGATGGAACAAGTGCCGCACATTGCACTGTTTTG TCAAGAGAACCGGCCTTCTATACCGTATGCTTTTTCCAAGTACTTACTACCAATTGTGGTCAGATACCTC GCAGACCAGAATAACCAGGTGAGGAAAACCAGTCAGGCAGCTTTGCTGGCTCTGCTGGAGCAGGAACTGA TTGAGCGATTTGACGTGGAGACCAAGGTGTGCCCTGTCCTCATAGACCTGACTGCCCCAGACAGCAATGA TGATGTGAAGACAGAGGCTGTGGCTATAATGTGCAAGATGGCCCCCATGGTTGGGAAGGACATTACGGAG CGCCTCATCCTCCCCAGGTTCTGCGAGATGTGCTGTGACTGTCGAATGTTTCATGTCCGGAAGGTCTGCG CTGCCAATTTTGGAGATATTTGCAGTGTAGTTGGCCAACAAGCAACAGAAGAAATGTTGCTGCCCAGGTT CTTCCAGCTGTGCTCTGATAACGTGTGGGGCGTCCGAAAGGCTTGTGCTGAGTGCTTCATGGCTGTCTCC TGTGCAACATGTCAAGAAATCCGACGGACAAAGTTATCAGCACTATTTATTAACTTGATCAGTGATCCTT CACGTTGGGTTCGCCAAGCAGCCTTTCAGTCTCTGGGGCCTTTCATATCCACGTTTGCTAATCCATCAAG CTCGGGCCAGTGCTTTAAAGATGAGAGCAAGAGCTCGGAAGACTCCTCGGCAGAAGACAAAGACAGGATG AGAGACAGTGATGTCGTAGAAGAAGAGCATCGCAGGCCAGAGGACGCTCCTTCAGACCTCAGTGCCCCTC ACCCCAGCGCCAGGCTGGAGAGCCTCGAAGGCTGTGCTGCCAAGACACCTGGGCACTCTGCAGGTGACGT GCCTGCTCCAGTGGACAGCTCCTTACTCTGTACTTTGTCCTCAGAGTCTCCTCAGGAAGCAGCTAGCAAT GATGAGAATGGTAGAAAGCCTGACACCAACAGCAAGTCTGCGTCCCGGCCAGACGCTGGCACCAGCTCCC CAGAGGCCACTCCCTTAGACCAGGACATGTTCAACTCCTTCCATTTCTGGAGGACTCCTCTACCTAAAAT AGATCTTGATAAAGAGCTCCAGCAGGACCCCGAGGAGAGACTCAGCCCAGAGAGAACAGGAGATGTACCT GCAGCCCCTCTACCAGGCCCTCCCAATATCACCATGGCTACCCGGAAGGAACTAGAAGAAATGATAGAAA ACCTTGAGCCACACATGGATGACCCGGATGTGAAAGCACAGGTGGAAGTGCTGTCGGCTGCCCTGCGAGC TTCCAGCCTGGATGCTCACGAAGAGACCGGTGGTGTAGAGCAGCGGAGTGAGCTACAAGATGAAGTGTGT GTCAGTGAGCTTCCAGACTGTAACATAAGTCACGACACTTGTGTGCCTTTGATCAGCGCTGCTGAGGAGA ATGTGGAGGCCACTCCTGATTATGTCCATGGAGGTGCAGACGTAAGCCCTGGTGATGGCTTCAGCCCGGA TGAAGATAGGAGACCCAAAGTCCAGGATGTTGTACCACAAGCGTTACTGGATCAGTACTTGTCAATGACC GACCCTTCTCGAGCACAGACAGTTGACACTGAGATCGCTAAGCACTGTGCATACAGTCTGCCGGGTGTGG CCCTGACTCTTGGCAGACAGAACTGGCACTGTTTGAGGGAGACTTATGAGACCCTAGCGTCGGACATGCA GTGGAAAGTTCGAAGGACTCTAGCCTTCTCCATCCATGAGCTTGCAGTGATCCTTGGGGACCAGCTGACA GCTGCAGACCTGGTTCCAATCTTCAATGGATTTTTAAAAGACCTCGATGAAGTCAGGATAGGTGTTCTTA AACACTTGCATGACTTTCTGAAGCTTCTTCACATTGATAAAAGAAGAGAGTACCTTTATCAGCTCCAGGA GTTTTTGGTCACAGACAACAGTAGAAATTGGCGCTTTCGAGCTGAACTGGCAGAACAGCTGATTTTACTT CTGGAGTTATACAGTCCCAGAGATGTTTATGATTACCTACGACCCATCGCTCTGAATCTGTGTGCAGACA AAGTTTCTTCAGTCCGCTGGATTTCCTACAAGTTGGTCAGTGAAATGGTGAAGAAACTCCACACAGCAAC ACCACCAACCTTCGGAGTAGATCTCATCAATGAGCTGGTAGAGAATTTTGGCAGGTGTCCAAAATGGTCT GGTCGGCAAGCCTTTGTCTTTGTCTGCCAGACTGTCATTGAAGACGACTGCCTCCCCATGGACCAGTTTG CTGTGCACTTGATGCCACATTTGCTGACCTTGGCAAATGACAGGGTTCCCAATGTTAGAGTGCTGCTTGC GAAAACCCTGCGACAGACTCTGCTGGAGAAAGAGTACTTTTTAGCCTCTGCCAGCTGTCACCAGGAGGCC GTGGAGCAGACAATCATGGCCCTGCAGATGGACCGAGACAGTGATGTCAAGTACTTTGCAAGCATCCACC CCTCCAGTACCAAACTCTCTGAAGATGCAATGAGCACAGCTTCCTCCACCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211287 representing BC029761 Red=Cloning site Green=Tags(s) MADLSLLQEDLPEDADGLDVDDYSSESDVIIIPSALDFVSQDEMLTPLGRLDKYAASENVFNRQMVARSL LDTLREVCDDERDCIAVLERISRLADDSEPTVRAELMEQVPHIALFCQENRPSIPYAFSKYLLPIVVRYL ADQNNQVRKTSQAALLALLEQELIERFDVETKVCPVLIDLTAPDSNDDVKTEAVAIMCKMAPMVGKDITE RLILPRFCEMCCDCRMFHVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECFMAVS CATCQEIRRTKLSALFINLISDPSRWVRQAAFQSLGPFISTFANPSSSGQCFKDESKSSEDSSAEDKDRM RDSDVVEEEHRRPEDAPSDLSAPHPSARLESLEGCAAKTPGHSAGDVPAPVDSSLLCTLSSESPQEAASN DENGRKPDTNSKSASRPDAGTSSPEATPLDQDMFNSFHFWRTPLPKIDLDKELQQDPEERLSPERTGDVP AAPLPGPPNITMATRKELEEMIENLEPHMDDPDVKAQVEVLSAALRASSLDAHEETGGVEQRSELQDEVC VSELPDCNISHDTCVPLISAAEENVEATPDYVHGGADVSPGDGFSPDEDRRPKVQDVVPQALLDQYLSMT DPSRAQTVDTEIAKHCAYSLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQLT AADLVPIFNGFLKDLDEVRIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLILL LELYSPRDVYDYLRPIALNLCADKVSSVRWISYKLVSEMVKKLHTATPPTFGVDLINELVENFGRCPKWS GRQAFVFVCQTVIEDDCLPMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKEYFLASASCHQEA VEQTIMALQMDRDSDVKYFASIHPSSTKLSEDAMSTASSTY TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC029761
ORF Size	2855 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029761, AAH29761
RefSeq Size	3739 bp
RefSeq ORF	2855 bp
Locus ID	70351
Gene Summary	Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3 (By similarity).[UniProtKB/Swiss-Prot Function]

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