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Prpf6 (NM_133701) Mouse Tagged ORF Clone
CAT＃: MG211260
- TrueORF®
Prpf6 (tGFP-tagged) - Mouse PRP6 pre-mRNA splicing factor 6 homolog (yeast) (Prpf6)
ORF Plasmid: DDK
"NM_133701" in other vectors (4)
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CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1190003A07Rik; 2610031L17Rik; ANT-1; U5-102K |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211260 representing NM_133701
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAAGAAGAAGAAGCCGTTCCTGGGCATGCCTGCGCCGCTGGGCTACGTACCCGGGCTGGGCAGGG GTGCCACTGGCTTCACCACCCGATCAGACATCGGGCCTGCTCGGGATGCAAATGATCCTGTGGATGATCG CCATGCCCCACCAGGGAAGAGAACTGTGGGGGACCAGATGAAGAAAAATCAGGCGGCTGATGATGATGAT GAAGATCTGAATGATACCAACTACGATGAGTTTAATGGCTATGCTGGGAGCCTCTTCTCAAGTGGGCCCT ATGAAAAAGATGATGAGGAAGCAGATGCTATTTATGCAGCTCTGGATAAAAGGATGGATGAGAGAAGAAA AGAGAGAAGAGAGCAAAGGGAGAAAGAAGAGATTGAGAAATACCGAATGGAACGTCCCAAAATCCAGCAG CAATTTTCAGACCTTAAGAGGAAGCTGGCAGAAGTCACAGAAGAAGAGTGGCTGAGCATCCCAGAAGTTG GCGATGCCAGAAACAAACGTCAACGGAACCCACGTTATGAGAAGCTGACTCCTGTTCCTGATAGTTTCTT TGCCAAACATTTACAGACTGGAGAGAACCATACTTCTGTGGATCCCCGACAGACTCAATTTGGAGGTCTG AACACACCCTACCCGGGAGGCCTGAACACTCCATACCCAGGTGGAATGACACCTGGATTGATGACACCTG GTACAGGGGAGCTGGACATGAGGAAGATTGGCCAGGCAAGGAATACCCTGATGGACATGAGGCTTAGCCA GGTATCTGACTCAGTGAGTGGGCAGACTGTTGTGGACCCCAAAGGCTACCTGACAGATCTAAATTCCATG ATCCCAACCCATGGCGGGGACATAAATGACATCAAGAAAGCACGACTACTTCTCAAATCTGTTCGAGAAA CAAACCCTCATCACCCACCAGCTTGGATTGCATCAGCTCGCCTGGAAGAAGTCACTGGAAAGTTACAAGT AGCTCGAAACCTTATTATGAAAGGGACAGAGATGTGCCCCAAGAGTGAAGATGTCTGGCTAGAAGCAGCT AGGTTACAGCCTGGGGACACAGCCAAGGCTGTGGTGGCACAAGCTGTCCGTCATCTCCCGCAGTCTGTTA GGATTTACATCAGAGCAGCAGAGCTAGAAACAGACATTCGAGCAAAGAAGCGTGTTCTTCGGAAAGCACT TGAGCATGTTCCAAACTCTGTTCGATTGTGGAAAGCAGCTGTTGAGCTTGAAGAGCCTGAAGATGCTAGA ATCATGCTGAGCCGAGCAGTAGAGTGCTGTCCCACCAGTGTAGAGCTATGGCTTGCTTTGGCAAGACTAG AAACTTATGAAAATGCTCGCAAGGTCTTAAATAAAGCACGTGAGAACATTCCTACAGATCGGCACATCTG GATCACAGCTGCCAAACTGGAGGAGGCCAATGGGAACACTCAGATGGTGGAAAAGATCATTGACCGAGCC ATCACCTCCCTACGGGCCAATGGTGTAGAGATCAACCGCGAACAGTGGATCCAGGATGCTGAGGAGTGTG ACAGGGCAGGGAGTGTGGCCACGTGCCAGGCTGTCATGCGTGCTGTAATCGGTATTGGGATTGAGGAGGA AGATCGCAAACACACCTGGATGGAAGACGCTGACAGTTGTGTGGCCCACAATGCCCTGGAGTGTGCACGA GCCATATATGCCTATGCCCTACAAGTCTTCCCCAGCAAGAAAAGTGTGTGGTTACGAGCTGCCTACTTTG AGAAGAATCATGGTACCAGGGAGTCTCTGGAAGCACTCTTGCAGAGGGCTGTAGCCCACTGCCCCAAGGC AGAGGTGCTATGGCTCATGGGTGCCAAGTCAAAGTGGCTGGCAGGGGATGTACCTGCAGCAAGGAGCATC CTGGCCCTGGCCTTTCAGGCCAACCCCAACAGCGAAGAGATCTGGTTGGCGGCTGTGAAGCTGGAGTCAG AGAACAATGAATACGAGCGAGCCCGGAGGCTACTGGCCAAGGCTCGGAGCAGCGCACCCACTGCCCGTGT ATTCATGAAGTCTGTGAAGCTGGAGTGGGTTCTGGGGAACATCTCTGCGGCGCAGGAGCTGTGTGAGGAG GCTCTGAGGCACTATGAAGATTTCCCCAAGCTGTGGATGATGAAGGGGCAGATTGAGGAGCAGGGAGAAC TGATGGAGAAGGCCCGGGAGGCCTACAACCAGGGGCTGAAGAAATGTCCTCATTCCACACCGCTTTGGCT CTTGCTTTCCCGACTAGAGGAAAAGATTGGGCAGCTGACACGTGCTCGGGCCATTTTAGAGAAATCTCGT CTGAAAAACCCAAAGAACCCTGGGCTATGGTTGGAGTCAGTGCGGCTGGAGTACCGGGCAGGGCTGAAGA ACATTGCCAATACCCTCATGGCCAAGGCGCTGCAGGAGTGCCCTAACTCTGGTATCCTGTGGTCTGAAGC TGTCTTCCTTGAGGCAAGGCCCCAGAGAAAGACCAAGAGTGTGGATGCCCTGAAGAAGTGTGAACATGAC CCCCATGTGCTTTTAGCTGTGGCTAAGTTGTTCTGGAGTGAAAGAAAGATCACCAAGGCTCGGGAGTGGT TCCATCGCACTGTGAAGATTGACTCAGATCTGGGGGATGCCTGGGCCTTCTTCTACAAATTTGAACTGCA GCATGGAACTGAGGAGCAACAGGAGGAGGTGAGGAAACGCTGTGAGAATGCAGAGCCCCGACATGGAGAG CTGTGGTGTGCTGTGTCCAAGGATATCACCAACTGGCAGAGGAAGATTGGGGAAATCCTAGTCCTGGTGG CCGCCCGCATCAAAAATACCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211260 representing NM_133701
Red=Cloning site Green=Tags(s) MNKKKKPFLGMPAPLGYVPGLGRGATGFTTRSDIGPARDANDPVDDRHAPPGKRTVGDQMKKNQAADDDD EDLNDTNYDEFNGYAGSLFSSGPYEKDDEEADAIYAALDKRMDERRKERREQREKEEIEKYRMERPKIQQ QFSDLKRKLAEVTEEEWLSIPEVGDARNKRQRNPRYEKLTPVPDSFFAKHLQTGENHTSVDPRQTQFGGL NTPYPGGLNTPYPGGMTPGLMTPGTGELDMRKIGQARNTLMDMRLSQVSDSVSGQTVVDPKGYLTDLNSM IPTHGGDINDIKKARLLLKSVRETNPHHPPAWIASARLEEVTGKLQVARNLIMKGTEMCPKSEDVWLEAA RLQPGDTAKAVVAQAVRHLPQSVRIYIRAAELETDIRAKKRVLRKALEHVPNSVRLWKAAVELEEPEDAR IMLSRAVECCPTSVELWLALARLETYENARKVLNKARENIPTDRHIWITAAKLEEANGNTQMVEKIIDRA ITSLRANGVEINREQWIQDAEECDRAGSVATCQAVMRAVIGIGIEEEDRKHTWMEDADSCVAHNALECAR AIYAYALQVFPSKKSVWLRAAYFEKNHGTRESLEALLQRAVAHCPKAEVLWLMGAKSKWLAGDVPAARSI LALAFQANPNSEEIWLAAVKLESENNEYERARRLLAKARSSAPTARVFMKSVKLEWVLGNISAAQELCEE ALRHYEDFPKLWMMKGQIEEQGELMEKAREAYNQGLKKCPHSTPLWLLLSRLEEKIGQLTRARAILEKSR LKNPKNPGLWLESVRLEYRAGLKNIANTLMAKALQECPNSGILWSEAVFLEARPQRKTKSVDALKKCEHD PHVLLAVAKLFWSERKITKAREWFHRTVKIDSDLGDAWAFFYKFELQHGTEEQQEEVRKRCENAEPRHGE LWCAVSKDITNWQRKIGEILVLVAARIKNTF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133701 |
| ORF Size | 2823 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133701.2, NP_598462.1 |
| RefSeq Size | 3071 bp |
| RefSeq ORF | 2826 bp |
| Locus ID | 68879 |
| UniProt ID | Q91YR7 |
| Gene Summary | Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222727 | Prpf6 (untagged) - Mouse PRP6 pre-mRNA splicing factor 6 homolog (yeast) (Prpf6), (10ug) |
CNY 12,070.00 |
|
| MR211260 | Prpf6 (Myc-DDK-tagged) - Mouse PRP6 pre-mRNA splicing factor 6 homolog (yeast) (Prpf6) |
CNY 6,896.00 |
|
| MR211260L3 | Lenti ORF clone of Prpf6 (Myc-DDK-tagged) - Mouse PRP6 pre-mRNA splicing factor 6 homolog (yeast) (Prpf6) |
CNY 10,170.00 |
|
| MR211260L4 | Lenti ORF clone of Prpf6 (mGFP-tagged) - Mouse PRP6 pre-mRNA splicing factor 6 homolog (yeast) (Prpf6) |
CNY 10,170.00 |
