Unc45b (BC084585) Mouse Tagged ORF Clone

CAT#: MG211221

  • TrueORF®

Unc45b (tGFP-tagged) - Mouse unc-45 homolog B (C. elegans) (cDNA clone MGC:91090 IMAGE:30429260)



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Mouse Protein / Lysate Request

CNY 9,030.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AA445617; Cmya4; D230041A13Rik; Unc45
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211221 representing BC084585
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGGCTGAAGCGGCACAGCTGAAGGAGGAGGGGAACCGGCATTTTCAGCTCCAGGATTACAAGG
CGGCCACCAAGAGCTACAGCCAGGCCCTGAAGCTGACCAAGGACAAGGCTCTGCTAGCTACACTCTACCG
GAATCGGGCAGCCTGCGGCCTGAAAATGGAGAGCTACGCCCAGGCAGCCTCGGATGCTTCCAGAGCCATT
GACATCAACTCTGCTGACATCAAAGCATTGTATCGGCGATGCCAGGCACTGGAGCATCTTGGCAAGTTGG
ACCAGGCATTCAAGGATGTGCAGCGCTGTGCTACCCTGGAGCCAAGGAACCAGAACTTCCAGGAGACACT
GCGGAGGCTCAACACCAGCATCCAAGAGCAGCTCCGTGTGCAGTTTTCTACAGACTCCAGGGTACAGACG
ATGTTTGAAATTCTTCTGAACGAAAACAGCGAGGCTGATAAGCGAGAGAAGGCTGCCAACAACCTCATTG
TCCTGGGCCGTGAAGAAGCGGGTGCTGAGAGGATCCTCCAGAGCAATGGAGTGGCTCTGCTGCTGCAGCT
TATGAACACCCAGCGGCCTGAGCTGCTGCTAGCGGCTGTGCGGACGCTGTCTGGCATGTGCAGTGGCCAC
AGAGCTAGGGCCACCGCCATCCTGCACGCAGTCCGCATAGACAGAATCTGCAGCCTCATGGCCCTGGAGA
ATGAAGAGATGTCCCTGGCTGTCTGCAACCTGCTCCAGGCCATCATTGACTCCCTGTCTGGAGAGGACAA
GAGAGAGCATCGTGGGAAGGAGGAGGCCTTGGTTCTAGATACCAAGAAGGACCTGAAGCAGATCACCAGC
CACCTGCTGGACATGCTGGTCAGTAAGAAAGTGTCTGGGCAGGGCAGAGACCAGGCACTGAACCTGCTTA
ATAAGAATGTCCCCAGGAAGGACCTGTCCATTCATGACAACTCACGCACCATCTATGTTGTAGATAACGG
TCTGAGGAAGATCCTGAAGGTGGTGGGGCAGGTTCCAGACCTGCCCTCCTGCCTACCCCTGACGGACAAC
ACCCGCATGCTGGCCTCCATCCTCATCAACAAGCTCTATGATGACCTGCGCTGTGACCCAGAGCGCGACC
ACTTCCGCAAGATCTGTGAGGAGTATATCACGAGCAAATTTGACCCCCAGGACATGGACAAGAATGTAAA
TGCCATCCAGACGGTGTCCGGCATCTTGCAGGGGCCCTTTGACCTGGGCAATCAGCTGCTGGGCATGAAA
GGTGTGATGGAGATGATGGTGGCTCTGTGTGGCTCGGAGCGCGAGGCAGACCAGCTGGTGGCTGTGGAGG
CTCTCATCCACGCCTCCACGAAGCTCAGCCGGGCCACCTTCATCATCACCAACGGAGTGACACTTCTTAA
GCAGATCTACAAGACCACAAAGAATGAGAAGATCAAGATCCGCACGCTGGTGGGGCTCTGTAAACTTGGC
TCAGCTGGAGGCTCCGACTATGGTCTCAGACAGTTTGCTGAAGGGTCAACAGAGAAGCTAGCCAAGCAGT
GTCGAAAGTGGCTGTGTAACACCGCCATAGACACCCGGACGCGGCGCTGGGCAGTGGAGGGCCTGGCCTA
CCTCACGCTGGACGCTGACGTGAAGGACGACTTTGTCCAGGACATCCCTGCTCTGCAGGCCATGTTTGAG
TTGGCCAAGACCAGTGACAAGACCATCCTCTACTCAGTGGCCAACACCCTGGTAAACTGCACCAACAGCT
ATGATGTCAAGGAGGTCGTCCCTGAGCTGGTGCAGCTGGCCAAGTTCTCCAAGCAACATGTGCCAGAGGA
GCACCCCAAGGACAAGAAGGACTTCGTTGACTTGCGGGTGAAACGTCTTCTGAAGGCAGGTGTCATATCT
GCACTGGCCTGCATGGTGAAGGCAGACAGTGCCATCCTCACAGACCAGACCAAGGAGCTGCTGGCCAGGG
TATTCCTGGCACTCTGTGACAATCCAAAGGACCGGGGCACCATTGTAGCTCAAGGTGGTGGCAAGGCCCT
GATCCCCCTGGCTTTGGAGGGCACAGATGTGGGCAAGGTGAAGGCAGCCCATGGGCTAGCCAAGATTGCT
GCTGTCTCCAACCCGGACATTGCCTTCCCTGGGGAGAGGGTATATGAGGTGGTGCGGCCCCTCGTGAGTC
TCCTGGACACACAGAGGGATGGGCTTCAGAACTATGAGGCTCTGCTGGGACTGACTAACCTGTCTGGGCG
CAGTGACAAACTCAGGCAGAAGATCTTCAAGGAGAAGGCCTTGCCAGACATCGAAAACTACATGTTTGAG
AACCATGACCAGCTACGGCAGGCAGCCACCGAATGCATGTGTAACATGGTGCTCAATAAGGAGGTTCAGG
AGAGGTTCCTGGCCGATGGGAATGATCGTCTGAAGCTGGTGGTGCTGCTCTGTGGGGAGGATGACCACAA
ACTGCAGAACGCAGCAGCTGGAGCTCTAGCCATGCTGACCGCGGCACATAAGAAACTGTGCCTCAAGATG
ACGGAGGTGACAACCCAGTGGCTGGAGATCCTACAGCGGCTTTGCCTGCATGACCAGTTGTCCGTGCAGC
ACCGGGGCCTGGTCATTGCCCACAACCTGCTATCAGCGGATGCTGAGCTGGCGAGGAAGCTGGTGGAGAG
CGAGCTGCTGGAGATCCTGACTGTGGTGGGAAAGCAGGAGCCAGATGAGAAGAGAGCGGCTGTGGTCCAG
ACAGCCCGGGAGTGTCTTATCAAGTGCATGGATTATGGCTTCATCAAGCCGGTGTCA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211221 representing BC084585
Red=Cloning site Green=Tags(s)

MAEAEAAQLKEEGNRHFQLQDYKAATKSYSQALKLTKDKALLATLYRNRAACGLKMESYAQAASDASRAI
DINSADIKALYRRCQALEHLGKLDQAFKDVQRCATLEPRNQNFQETLRRLNTSIQEQLRVQFSTDSRVQT
MFEILLNENSEADKREKAANNLIVLGREEAGAERILQSNGVALLLQLMNTQRPELLLAAVRTLSGMCSGH
RARATAILHAVRIDRICSLMALENEEMSLAVCNLLQAIIDSLSGEDKREHRGKEEALVLDTKKDLKQITS
HLLDMLVSKKVSGQGRDQALNLLNKNVPRKDLSIHDNSRTIYVVDNGLRKILKVVGQVPDLPSCLPLTDN
TRMLASILINKLYDDLRCDPERDHFRKICEEYITSKFDPQDMDKNVNAIQTVSGILQGPFDLGNQLLGMK
GVMEMMVALCGSEREADQLVAVEALIHASTKLSRATFIITNGVTLLKQIYKTTKNEKIKIRTLVGLCKLG
SAGGSDYGLRQFAEGSTEKLAKQCRKWLCNTAIDTRTRRWAVEGLAYLTLDADVKDDFVQDIPALQAMFE
LAKTSDKTILYSVANTLVNCTNSYDVKEVVPELVQLAKFSKQHVPEEHPKDKKDFVDLRVKRLLKAGVIS
ALACMVKADSAILTDQTKELLARVFLALCDNPKDRGTIVAQGGGKALIPLALEGTDVGKVKAAHGLAKIA
AVSNPDIAFPGERVYEVVRPLVSLLDTQRDGLQNYEALLGLTNLSGRSDKLRQKIFKEKALPDIENYMFE
NHDQLRQAATECMCNMVLNKEVQERFLADGNDRLKLVVLLCGEDDHKLQNAAAGALAMLTAAHKKLCLKM
TEVTTQWLEILQRLCLHDQLSVQHRGLVIAHNLLSADAELARKLVESELLEILTVVGKQEPDEKRAAVVQ
TARECLIKCMDYGFIKPVS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC084585
ORF Size 2789 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC084585, AAH84585
RefSeq Size 3610 bp
RefSeq ORF 2789 bp
Locus ID 217012
Gene Summary Acts as a co-chaperone for HSP90 and is required for proper folding of the myosin motor domain. Plays a role in sarcomere formation during muscle cell development (PubMed:12356907, PubMed:18326487, PubMed:18478096). Is necessary for normal early lens development (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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