Slc8a3 (BC052435) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AW742262; Ncx3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211189 representing BC052435 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTGGTTACGGCTGCAGCCTCTCACCTCTGCCTTCCTCCATTTTGGGCTGGTTACTTTTGTGCTCT TCCTGAATTGTCTTCGAGCAGAGGCTGGTGACTCGGGGGATGTGCCCAGTGCAGGGCAGAACAATGAGTC CTGTTCGGGGTCATCAGACTGCAAGGAGGGTGTCATTTTGCCAATCTGGTATCCAGAGAACCCTTCCCTT GGGGACAAGATTGCCAGGGTCATTGTCTATTTTGTGGCCCTGATATACATGTTTCTTGGGGTGTCTATCA TTGCTGACCGATTCATGGCATCTATTGAAGTCATTACTTCCCAAGAGAGGGAAGTGACCATCAAGAAGCC CAATGGAGAGACCAGCACAACTACAATTCGGGTATGGAATGAAACTGTCTCCAATCTGACCCTGATGGCC CTGGGCTCTTCTGCTCCAGAGATTCTCCTGTCTTTAATTGAGGTGTGTGGTCACGGGTTCATTGCTGGTG ATCTGGGACCATCTACCATCGTTGGCAGTGCAGCCTTCAACATGTTCATCATCATTGGCATCTGTGTCTA TGTGATCCCAGATGGGGAGACTCGAAAGATCAAGCACCTGCGAGTCTTCTTCGTCACGGCTGCTTGGAGC ATCTTCGCCTACATTTGGCTCTATATGATCCTGGCAGTCTTCTCTCCTGGTGTGGTCCAGGTTTGGGAAG GCCTCCTTACTCTCTTCTTCTTTCCCGTGTGTGTCCTGCTGGCTTGGGTGGCAGATAAGCGACTGCTCTT CTACAAATACATGCACAAAAAATACCGCACAGATAAACACCGAGGAATTATCATTGAGACAGAGGGTGAC CACCCTAAGGGCATTGAGATGGATGGGAAAATGATGAATTCTCACTTTCTAGATGGGAACTTTACACCTT TGGAAGGAAAGGAGGTAGATGAATCTCGCAGGGAAATGATCCGGATTCTAAAGGATCTGAAACAAAAACA CCCAGAAAAGGACCTAGATCAGCTGGTGGAGATGGCCAATTACTATGCTCTTTCCCATCAACAGAAGAGC CGTGCTTTCTACCGCATCCAAGCCACCCGGATGATGACTGGTGCGGGCAATATACTTAAGAAGCATGCAG CCGAGCAAGCCAAGAAGACCTCCAGCATGAGCGAGGTGCATACCGATGAGCCGGAGGACTTTGCCTCTAA GGTCTTCTTTGACCCATGTTCTTATCAGTGCCTGGAGAACTGTGGAGCTGTCCTCCTGACCGTGGTGAGG AAAGGGGGAGATATATCCAAGACCATGTACGTGGACTACAAAACAGAGGACGGCTCCGCCAATGCAGGGG CAGACTATGAGTTCACAGAGGGCACTGTGGTTCTGAAGCCAGGAGAGACCCAGAAGGAGTTCTCTGTGGG CATCATTGATGATGACATTTTTGAGGAGGATGAACACTTCTTTGTGAGGCTGAGCAATGTCCGTGTAGAA GAGGAGCAGCTGGCGGAGGGGATGCTCCCAGCAATACTCAATAGTCTTCCTTTGCCTCGGGCTGTCCTGG CCTCCCCTTGTGTGGCCACAGTAACCATCTTGGATGATGACCATGCAGGAATTTTCACTTTTGAATGTGA TACCATTCATGTCAGTGAAAGTATTGGTGTTATGGAAGTCAAGGTTTTGAGGACATCAGGTGCCAGGGGC ACAGTCATCGTCCCTTTTAGGACAGTAGAAGGGACAGCCAAGGGTGGTGGCGAGGACTTTGAAGATGCAT ATGGGGAGCTGGAGTTCAAGAATGATGAAACAGTGAAAACCATAAGGGTTAAAATAGTAGATGAGGAGGA GTACGAGAGGCAAGAGAATTTCTTCATTGCCCTTGGTGAACCGAAATGGATGGAACGTGGAATATCAGAA GTGACAGACAGGAAGCTGACTGTGGAGGAAGAGGAAGCCAAGAGAATAGCAGAGATGGGAAAGCCAGTAT TGGGTGAACACCCCAAACTAGAGGTCATCATTGAAGAGTCCTATGAGTTCAAGAGTACAGTGGATAAGCT GATCAAGAAGACAAACCTGGCATTGGTTGTGGGGACCCATTCCTGGAGGGACCAGTTCATGGAAGCCATC ACTGTTAGTGCAGGAGGGGATGAGGATGAAGACGAATCTGGAGAGGAGAGGCTGCCATCCTGCTTTGACT ACGTCATGCACTTCCTGACGGTCTTCTGGAAGGTGCTCTTTGCCTGTGTGCCCCCCACAGAGTACTGCCA CGGCTGGGCCTGCTTCGTGGTCTCCATCCTCATTATTGGCATGCTCACCGCCATCATCGGGGACCTGGCC TCTCACTTCGGCTGCACCATCGGGCTCAAGGATTCGGTCACAGCTGTTGTTTTTGTGGCATTCGGCACCT CTGTGCCAGATACATTTGCCAGCAAAGCCGCTGCCCTGCAGGACGTGTATGCAGATGCTTCCATTGGCAA CGTCACAGGCAGTAATGCCGTCAATGTCTTCCTGGGTATTGGTTTGGCCTGGTCCGTGGCCGCCATCTAC TGGGCCATGCAGGGACAGGAGTTCCATGTGTCCGCTGGCACTCTGGCCTTCTCGGTCACTCTTTTCACCA TCTTTGCATTTGTCTGCCTCAGTGTGCTCTTGTATCGTCGGCGGCCCCATCTGGGCGGGGAGCTCGGAGG TCCTCGTGGCTGCAAGCTTGCCACGACATGGCTCTTTGTGAGCCTATGGCTCCTCTACATACTATTTGCC ACGCTGGAGGCCTACTGCTACATCAAGGGGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211189 representing BC052435 Red=Cloning site Green=Tags(s) MAWLRLQPLTSAFLHFGLVTFVLFLNCLRAEAGDSGDVPSAGQNNESCSGSSDCKEGVILPIWYPENPSL GDKIARVIVYFVALIYMFLGVSIIADRFMASIEVITSQEREVTIKKPNGETSTTTIRVWNETVSNLTLMA LGSSAPEILLSLIEVCGHGFIAGDLGPSTIVGSAAFNMFIIIGICVYVIPDGETRKIKHLRVFFVTAAWS IFAYIWLYMILAVFSPGVVQVWEGLLTLFFFPVCVLLAWVADKRLLFYKYMHKKYRTDKHRGIIIETEGD HPKGIEMDGKMMNSHFLDGNFTPLEGKEVDESRREMIRILKDLKQKHPEKDLDQLVEMANYYALSHQQKS RAFYRIQATRMMTGAGNILKKHAAEQAKKTSSMSEVHTDEPEDFASKVFFDPCSYQCLENCGAVLLTVVR KGGDISKTMYVDYKTEDGSANAGADYEFTEGTVVLKPGETQKEFSVGIIDDDIFEEDEHFFVRLSNVRVE EEQLAEGMLPAILNSLPLPRAVLASPCVATVTILDDDHAGIFTFECDTIHVSESIGVMEVKVLRTSGARG TVIVPFRTVEGTAKGGGEDFEDAYGELEFKNDETVKTIRVKIVDEEEYERQENFFIALGEPKWMERGISE VTDRKLTVEEEEAKRIAEMGKPVLGEHPKLEVIIEESYEFKSTVDKLIKKTNLALVVGTHSWRDQFMEAI TVSAGGDEDEDESGEERLPSCFDYVMHFLTVFWKVLFACVPPTEYCHGWACFVVSILIIGMLTAIIGDLA SHFGCTIGLKDSVTAVVFVAFGTSVPDTFASKAAALQDVYADASIGNVTGSNAVNVFLGIGLAWSVAAIY WAMQGQEFHVSAGTLAFSVTLFTIFAFVCLSVLLYRRRPHLGGELGGPRGCKLATTWLFVSLWLLYILFA TLEAYCYIKGF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC052435
ORF Size	2765 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC052435, AAH52435
RefSeq Size	4640 bp
RefSeq ORF	2765 bp
Locus ID	110893
Gene Summary	Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells, both in muscle and in brain (PubMed:14722618, PubMed:21593315). In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A3 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:14722618, PubMed:21593315). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle (PubMed:14722618). In neurons, contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory (PubMed:21593315). Required for normal oligodendrocyte differentiation and for normal myelination (PubMed:21959935). Mediates Ca(2+) efflux from mitochondria and contributes to mitochondrial Ca(2+) ion homeostasis (PubMed:24616101). Isoform 1 displays higher calcium exchanger activity than isoform 2, probably because isoform 1 has a lower threshold for activation by cytoplasmic Ca(2+) (PubMed:24616101).[UniProtKB/Swiss-Prot Function]

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