Slc8a3 (BC052435) Mouse Tagged ORF Clone

CAT#: MG211189

  • TrueORF®

Slc8a3 (tGFP-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 3 (cDNA clone MGC:63358 IMAGE:6837128)



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Mouse Protein / Lysate Request

CNY 10,170.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AW742262; Ncx3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211189 representing BC052435
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTGGTTACGGCTGCAGCCTCTCACCTCTGCCTTCCTCCATTTTGGGCTGGTTACTTTTGTGCTCT
TCCTGAATTGTCTTCGAGCAGAGGCTGGTGACTCGGGGGATGTGCCCAGTGCAGGGCAGAACAATGAGTC
CTGTTCGGGGTCATCAGACTGCAAGGAGGGTGTCATTTTGCCAATCTGGTATCCAGAGAACCCTTCCCTT
GGGGACAAGATTGCCAGGGTCATTGTCTATTTTGTGGCCCTGATATACATGTTTCTTGGGGTGTCTATCA
TTGCTGACCGATTCATGGCATCTATTGAAGTCATTACTTCCCAAGAGAGGGAAGTGACCATCAAGAAGCC
CAATGGAGAGACCAGCACAACTACAATTCGGGTATGGAATGAAACTGTCTCCAATCTGACCCTGATGGCC
CTGGGCTCTTCTGCTCCAGAGATTCTCCTGTCTTTAATTGAGGTGTGTGGTCACGGGTTCATTGCTGGTG
ATCTGGGACCATCTACCATCGTTGGCAGTGCAGCCTTCAACATGTTCATCATCATTGGCATCTGTGTCTA
TGTGATCCCAGATGGGGAGACTCGAAAGATCAAGCACCTGCGAGTCTTCTTCGTCACGGCTGCTTGGAGC
ATCTTCGCCTACATTTGGCTCTATATGATCCTGGCAGTCTTCTCTCCTGGTGTGGTCCAGGTTTGGGAAG
GCCTCCTTACTCTCTTCTTCTTTCCCGTGTGTGTCCTGCTGGCTTGGGTGGCAGATAAGCGACTGCTCTT
CTACAAATACATGCACAAAAAATACCGCACAGATAAACACCGAGGAATTATCATTGAGACAGAGGGTGAC
CACCCTAAGGGCATTGAGATGGATGGGAAAATGATGAATTCTCACTTTCTAGATGGGAACTTTACACCTT
TGGAAGGAAAGGAGGTAGATGAATCTCGCAGGGAAATGATCCGGATTCTAAAGGATCTGAAACAAAAACA
CCCAGAAAAGGACCTAGATCAGCTGGTGGAGATGGCCAATTACTATGCTCTTTCCCATCAACAGAAGAGC
CGTGCTTTCTACCGCATCCAAGCCACCCGGATGATGACTGGTGCGGGCAATATACTTAAGAAGCATGCAG
CCGAGCAAGCCAAGAAGACCTCCAGCATGAGCGAGGTGCATACCGATGAGCCGGAGGACTTTGCCTCTAA
GGTCTTCTTTGACCCATGTTCTTATCAGTGCCTGGAGAACTGTGGAGCTGTCCTCCTGACCGTGGTGAGG
AAAGGGGGAGATATATCCAAGACCATGTACGTGGACTACAAAACAGAGGACGGCTCCGCCAATGCAGGGG
CAGACTATGAGTTCACAGAGGGCACTGTGGTTCTGAAGCCAGGAGAGACCCAGAAGGAGTTCTCTGTGGG
CATCATTGATGATGACATTTTTGAGGAGGATGAACACTTCTTTGTGAGGCTGAGCAATGTCCGTGTAGAA
GAGGAGCAGCTGGCGGAGGGGATGCTCCCAGCAATACTCAATAGTCTTCCTTTGCCTCGGGCTGTCCTGG
CCTCCCCTTGTGTGGCCACAGTAACCATCTTGGATGATGACCATGCAGGAATTTTCACTTTTGAATGTGA
TACCATTCATGTCAGTGAAAGTATTGGTGTTATGGAAGTCAAGGTTTTGAGGACATCAGGTGCCAGGGGC
ACAGTCATCGTCCCTTTTAGGACAGTAGAAGGGACAGCCAAGGGTGGTGGCGAGGACTTTGAAGATGCAT
ATGGGGAGCTGGAGTTCAAGAATGATGAAACAGTGAAAACCATAAGGGTTAAAATAGTAGATGAGGAGGA
GTACGAGAGGCAAGAGAATTTCTTCATTGCCCTTGGTGAACCGAAATGGATGGAACGTGGAATATCAGAA
GTGACAGACAGGAAGCTGACTGTGGAGGAAGAGGAAGCCAAGAGAATAGCAGAGATGGGAAAGCCAGTAT
TGGGTGAACACCCCAAACTAGAGGTCATCATTGAAGAGTCCTATGAGTTCAAGAGTACAGTGGATAAGCT
GATCAAGAAGACAAACCTGGCATTGGTTGTGGGGACCCATTCCTGGAGGGACCAGTTCATGGAAGCCATC
ACTGTTAGTGCAGGAGGGGATGAGGATGAAGACGAATCTGGAGAGGAGAGGCTGCCATCCTGCTTTGACT
ACGTCATGCACTTCCTGACGGTCTTCTGGAAGGTGCTCTTTGCCTGTGTGCCCCCCACAGAGTACTGCCA
CGGCTGGGCCTGCTTCGTGGTCTCCATCCTCATTATTGGCATGCTCACCGCCATCATCGGGGACCTGGCC
TCTCACTTCGGCTGCACCATCGGGCTCAAGGATTCGGTCACAGCTGTTGTTTTTGTGGCATTCGGCACCT
CTGTGCCAGATACATTTGCCAGCAAAGCCGCTGCCCTGCAGGACGTGTATGCAGATGCTTCCATTGGCAA
CGTCACAGGCAGTAATGCCGTCAATGTCTTCCTGGGTATTGGTTTGGCCTGGTCCGTGGCCGCCATCTAC
TGGGCCATGCAGGGACAGGAGTTCCATGTGTCCGCTGGCACTCTGGCCTTCTCGGTCACTCTTTTCACCA
TCTTTGCATTTGTCTGCCTCAGTGTGCTCTTGTATCGTCGGCGGCCCCATCTGGGCGGGGAGCTCGGAGG
TCCTCGTGGCTGCAAGCTTGCCACGACATGGCTCTTTGTGAGCCTATGGCTCCTCTACATACTATTTGCC
ACGCTGGAGGCCTACTGCTACATCAAGGGGTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211189 representing BC052435
Red=Cloning site Green=Tags(s)

MAWLRLQPLTSAFLHFGLVTFVLFLNCLRAEAGDSGDVPSAGQNNESCSGSSDCKEGVILPIWYPENPSL
GDKIARVIVYFVALIYMFLGVSIIADRFMASIEVITSQEREVTIKKPNGETSTTTIRVWNETVSNLTLMA
LGSSAPEILLSLIEVCGHGFIAGDLGPSTIVGSAAFNMFIIIGICVYVIPDGETRKIKHLRVFFVTAAWS
IFAYIWLYMILAVFSPGVVQVWEGLLTLFFFPVCVLLAWVADKRLLFYKYMHKKYRTDKHRGIIIETEGD
HPKGIEMDGKMMNSHFLDGNFTPLEGKEVDESRREMIRILKDLKQKHPEKDLDQLVEMANYYALSHQQKS
RAFYRIQATRMMTGAGNILKKHAAEQAKKTSSMSEVHTDEPEDFASKVFFDPCSYQCLENCGAVLLTVVR
KGGDISKTMYVDYKTEDGSANAGADYEFTEGTVVLKPGETQKEFSVGIIDDDIFEEDEHFFVRLSNVRVE
EEQLAEGMLPAILNSLPLPRAVLASPCVATVTILDDDHAGIFTFECDTIHVSESIGVMEVKVLRTSGARG
TVIVPFRTVEGTAKGGGEDFEDAYGELEFKNDETVKTIRVKIVDEEEYERQENFFIALGEPKWMERGISE
VTDRKLTVEEEEAKRIAEMGKPVLGEHPKLEVIIEESYEFKSTVDKLIKKTNLALVVGTHSWRDQFMEAI
TVSAGGDEDEDESGEERLPSCFDYVMHFLTVFWKVLFACVPPTEYCHGWACFVVSILIIGMLTAIIGDLA
SHFGCTIGLKDSVTAVVFVAFGTSVPDTFASKAAALQDVYADASIGNVTGSNAVNVFLGIGLAWSVAAIY
WAMQGQEFHVSAGTLAFSVTLFTIFAFVCLSVLLYRRRPHLGGELGGPRGCKLATTWLFVSLWLLYILFA
TLEAYCYIKGF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC052435
ORF Size 2765 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC052435, AAH52435
RefSeq Size 4640 bp
RefSeq ORF 2765 bp
Locus ID 110893
Gene Summary Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells, both in muscle and in brain (PubMed:14722618, PubMed:21593315). In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A3 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:14722618, PubMed:21593315). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle (PubMed:14722618). In neurons, contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory (PubMed:21593315). Required for normal oligodendrocyte differentiation and for normal myelination (PubMed:21959935). Mediates Ca(2+) efflux from mitochondria and contributes to mitochondrial Ca(2+) ion homeostasis (PubMed:24616101). Isoform 1 displays higher calcium exchanger activity than isoform 2, probably because isoform 1 has a lower threshold for activation by cytoplasmic Ca(2+) (PubMed:24616101).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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