Eif3s8 (BC025032) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	NipilA3, NIPIL(A3), 110kDa, MGC36637
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211151 representing BC025032 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCGATTCTTCACCACCGGCTCGGACAGTGAGTCGGAGTCGTCCCTGTCCGGGGAGGAGCTCGTCA CCAAACCGGTCTCCGGGAACTACGGCAAACAGCCTTTGTTACTGAGCGAGGATGAGGAGGATACAAAGAG AGTTGTCCGAAGCGCCAAGGACAAGAGGTTTGAGGAGCTGACCAACCTCATACGAACCATCCGTAATGCC ATGAAGATTCGAGATGTCACCAAGTGTCTAGAAGAGTTTGAGCTCCTGGGAAAGGCCTATGGGAAAGCCA AGAGTATTGTGGATAAGGAAGGTGTCCCCCGATTCTACATCCGAATACTGACTGACCTGGAGGACTATCT TAATGAGCTTTGGGAAGATAAAGAAGGAAAGAAGAAGATGAATAAAAACAACGCCAAGGCTCTGAGTACC TTGCGCCAGAAGATCCGGAAATACAACCGAGATTTCGAGTCACACATCACCAACTACAAGCAGAACCCTG AGCAGTCTGCAGATGAAGATGCAGAAAAGAATGAGGAAGACTCCGAAGGCTCTTCAGATGAGGATGAGGA CGAGGACGGAGTCGGTAATACGACTTTCTTGAAGAAGAAGCAAGAATCTTCTGGAGAGAGTCGGAAGTTC CACAAAAAGATGGAAGATGATGATGAGGACTCTGAAGATTCTGAAGATGAAGAATGGGACACCAGCTCCA CATCTTCTGATTCAGACTCTGAGGAAGAAGAAGGAAAACAAACTGTTCTGGCCTCAAAGTTCCTTAAAAA GGCACCCACTACAGAGGAGGACAAAAAAGCAGCTGAAAAGAAACGGGAAGACAAAGCCAAGAAGAAGCAC GATAGGAAGTCTAAGCGCCTGGATGAGGAAGAGGAGGACAATGAGGGCGGGGAGTGGGAACGGGTCCGAG GTGGCGTACCACTTGTTAAGGAAAAGCCAAAAATGTTTGCCAAGGGAACTGAGATCACTCATGCTGTTGT CATCAAGAAACTGAATGAGATTCTACAGGTCAGAGGCAAGAAGGGAACAGACCGTGCCACTCAGATTGAA TTGCTCCAGCTGCTGGTTCAGATTGCTGCTGAAAACAACCTGGGGGTGGGCGTCATTGTCAAGATCAAGT TCAATATCATTGCCTCTCTCTACGATTACAACCCTAACCTGGCCACGTACATGAAGCCAGAGATGTGGCA GATGTGCCTAGACTGCATCAATGAACTGATGGATACGTTGGTTGCACATTCCAACATCTTTGTTGGAGAG AACATTTTGGAAGAGAGTGAGAACTTACACAACTTTGATCAGCCACTGCGTGTACGAGGCTGCATCCTAA CTTTGGTGGAGCGAATGGATGAAGAATTTACCAAAATAATGCAAAATACTGATCCTCACTCCCAAGAGTA TGTGGAGCACCTGAAGGATGAGGCACAAGTGTGTGCCATCATTGAGCGAGTGCAGCGCTACCTGGAGGAG AAAGGTACCACTGAGGAGATCTGCCAGATCTACTTAAGGCGCATCCTGCACACGTACTACAAGTTTGACT ACAAGGCCCATCAGCGGCAGCTTACTCCTCCTGAAGGATCCTCAAAGTCTGAGCAAGACCAGGCAGAAAA TGAGGGTGAGGACTCAGCTGTGCTAATGGAAAGACTGTGCAAGTACATCTATGCCAAGGACCGTACAGAC CGGATCCGTACCTGTGCCATCCTCTGCCATATCTACCATCATGCGCTCCACTCCCGCTGGTATCAGGCCC GTGACCTCATGCTCATGAGCCACCTACAGGACAACATTCAGCACGCAGACCCGCCGGTGCAGATCCTGTA TAACCGTACTATGGTGCAACTGGGCATCTGTGCTTTCCGCCAAGGCCTGACAAAGGATGCACACAATGCA CTTCTGGATATTCAGTCAAGTGGTCGTGCCAAGGAGCTTCTAGGTCAGGGTCTGCTGCTGCGCAGCCTGC AGGAGCGAAATCAGGAACAGGAAAAGGTAGAGCGACGCCGGCAGGTGCCCTTTCACCTGCACATCAACCT GGAGCTGCTGGAGTGTGTCTATCTGGTGTCAGCTATGCTCCTGGAGATCCCCTACATGGCTGCCCATGAG AGCGATGCCCGCCGACGCATGATCAGCAAGCAGTTCCACCACCAACTGCGGGTGGGCGAGCGGCAGCCCC TGCTAGGTCCTCCCGAGTCAATGAGGGAGCATGTGGTCGCTGCCTCCAAGGCCATGAAGATGGGCGACTG GAAGACCTGCCACAGTTTCATCATTAATGAAAAGATGAATGGGAAAGTGTGGGACCTTTTCCCTGAGGCT GACAAAGTTCGCACCATGCTAGTTCGGAAGATCCAGGAAGAGTCTCTGAGGACCTACCTTTTTACCTACA GCAGTGTCTATGACTCAATCAGTATGGAGACACTATCAGATATGTTTGAGCTGGATCTACCCACTGTTCA CTCCATCATCAGCAAGATGATCATTAACGAAGAATTGATGGCTTCCCTGGACCAGCCGACACAGACTGTG GTGATGCACCGTACTGAGCCCACTGCCCAGCAGAACTTGGCTCTGCAGCTGGCTGAGAAGCTTGGCAGCC TAGTGGAGAATAATGAACGGGTGTTTGACCATAAGCAGGGAACCTATGGTGGCTATTTCCGAGACCAAAA GGATGGCTACCGAAAAAATCTAGGCTATATGCGCCGTGGTGGCTACCGCCAGCAGCAGTCTCAGACAGCC TAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211151 representing BC025032 Red=Cloning site Green=Tags(s) MSRFFTTGSDSESESSLSGEELVTKPVSGNYGKQPLLLSEDEEDTKRVVRSAKDKRFEELTNLIRTIRNA MKIRDVTKCLEEFELLGKAYGKAKSIVDKEGVPRFYIRILTDLEDYLNELWEDKEGKKKMNKNNAKALST LRQKIRKYNRDFESHITNYKQNPEQSADEDAEKNEEDSEGSSDEDEDEDGVGNTTFLKKKQESSGESRKF HKKMEDDDEDSEDSEDEEWDTSSTSSDSDSEEEEGKQTVLASKFLKKAPTTEEDKKAAEKKREDKAKKKH DRKSKRLDEEEEDNEGGEWERVRGGVPLVKEKPKMFAKGTEITHAVVIKKLNEILQVRGKKGTDRATQIE LLQLLVQIAAENNLGVGVIVKIKFNIIASLYDYNPNLATYMKPEMWQMCLDCINELMDTLVAHSNIFVGE NILEESENLHNFDQPLRVRGCILTLVERMDEEFTKIMQNTDPHSQEYVEHLKDEAQVCAIIERVQRYLEE KGTTEEICQIYLRRILHTYYKFDYKAHQRQLTPPEGSSKSEQDQAENEGEDSAVLMERLCKYIYAKDRTD RIRTCAILCHIYHHALHSRWYQARDLMLMSHLQDNIQHADPPVQILYNRTMVQLGICAFRQGLTKDAHNA LLDIQSSGRAKELLGQGLLLRSLQERNQEQEKVERRRQVPFHLHINLELLECVYLVSAMLLEIPYMAAHE SDARRRMISKQFHHQLRVGERQPLLGPPESMREHVVAASKAMKMGDWKTCHSFIINEKMNGKVWDLFPEA DKVRTMLVRKIQEESLRTYLFTYSSVYDSISMETLSDMFELDLPTVHSIISKMIINEELMASLDQPTQTV VMHRTEPTAQQNLALQLAEKLGSLVENNERVFDHKQGTYGGYFRDQKDGYRKNLGYMRRGGYRQQQSQTA Y TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC025032
ORF Size	2735 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC025032, AAH25032
RefSeq Size	2923 bp
RefSeq ORF	2735 bp
Locus ID	56347
Gene Summary	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.[UniProtKB/Swiss-Prot Function]

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