Eif3s8 (BC025032) Mouse Tagged ORF Clone

CAT#: MG211151

  • TrueORF®

Eif3s8 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 3, subunit 8 (cDNA clone MGC:36328 IMAGE:4947435)



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Mouse Protein / Lysate Request

CNY 8,650.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms NipilA3, NIPIL(A3), 110kDa, MGC36637
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211151 representing BC025032
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCGATTCTTCACCACCGGCTCGGACAGTGAGTCGGAGTCGTCCCTGTCCGGGGAGGAGCTCGTCA
CCAAACCGGTCTCCGGGAACTACGGCAAACAGCCTTTGTTACTGAGCGAGGATGAGGAGGATACAAAGAG
AGTTGTCCGAAGCGCCAAGGACAAGAGGTTTGAGGAGCTGACCAACCTCATACGAACCATCCGTAATGCC
ATGAAGATTCGAGATGTCACCAAGTGTCTAGAAGAGTTTGAGCTCCTGGGAAAGGCCTATGGGAAAGCCA
AGAGTATTGTGGATAAGGAAGGTGTCCCCCGATTCTACATCCGAATACTGACTGACCTGGAGGACTATCT
TAATGAGCTTTGGGAAGATAAAGAAGGAAAGAAGAAGATGAATAAAAACAACGCCAAGGCTCTGAGTACC
TTGCGCCAGAAGATCCGGAAATACAACCGAGATTTCGAGTCACACATCACCAACTACAAGCAGAACCCTG
AGCAGTCTGCAGATGAAGATGCAGAAAAGAATGAGGAAGACTCCGAAGGCTCTTCAGATGAGGATGAGGA
CGAGGACGGAGTCGGTAATACGACTTTCTTGAAGAAGAAGCAAGAATCTTCTGGAGAGAGTCGGAAGTTC
CACAAAAAGATGGAAGATGATGATGAGGACTCTGAAGATTCTGAAGATGAAGAATGGGACACCAGCTCCA
CATCTTCTGATTCAGACTCTGAGGAAGAAGAAGGAAAACAAACTGTTCTGGCCTCAAAGTTCCTTAAAAA
GGCACCCACTACAGAGGAGGACAAAAAAGCAGCTGAAAAGAAACGGGAAGACAAAGCCAAGAAGAAGCAC
GATAGGAAGTCTAAGCGCCTGGATGAGGAAGAGGAGGACAATGAGGGCGGGGAGTGGGAACGGGTCCGAG
GTGGCGTACCACTTGTTAAGGAAAAGCCAAAAATGTTTGCCAAGGGAACTGAGATCACTCATGCTGTTGT
CATCAAGAAACTGAATGAGATTCTACAGGTCAGAGGCAAGAAGGGAACAGACCGTGCCACTCAGATTGAA
TTGCTCCAGCTGCTGGTTCAGATTGCTGCTGAAAACAACCTGGGGGTGGGCGTCATTGTCAAGATCAAGT
TCAATATCATTGCCTCTCTCTACGATTACAACCCTAACCTGGCCACGTACATGAAGCCAGAGATGTGGCA
GATGTGCCTAGACTGCATCAATGAACTGATGGATACGTTGGTTGCACATTCCAACATCTTTGTTGGAGAG
AACATTTTGGAAGAGAGTGAGAACTTACACAACTTTGATCAGCCACTGCGTGTACGAGGCTGCATCCTAA
CTTTGGTGGAGCGAATGGATGAAGAATTTACCAAAATAATGCAAAATACTGATCCTCACTCCCAAGAGTA
TGTGGAGCACCTGAAGGATGAGGCACAAGTGTGTGCCATCATTGAGCGAGTGCAGCGCTACCTGGAGGAG
AAAGGTACCACTGAGGAGATCTGCCAGATCTACTTAAGGCGCATCCTGCACACGTACTACAAGTTTGACT
ACAAGGCCCATCAGCGGCAGCTTACTCCTCCTGAAGGATCCTCAAAGTCTGAGCAAGACCAGGCAGAAAA
TGAGGGTGAGGACTCAGCTGTGCTAATGGAAAGACTGTGCAAGTACATCTATGCCAAGGACCGTACAGAC
CGGATCCGTACCTGTGCCATCCTCTGCCATATCTACCATCATGCGCTCCACTCCCGCTGGTATCAGGCCC
GTGACCTCATGCTCATGAGCCACCTACAGGACAACATTCAGCACGCAGACCCGCCGGTGCAGATCCTGTA
TAACCGTACTATGGTGCAACTGGGCATCTGTGCTTTCCGCCAAGGCCTGACAAAGGATGCACACAATGCA
CTTCTGGATATTCAGTCAAGTGGTCGTGCCAAGGAGCTTCTAGGTCAGGGTCTGCTGCTGCGCAGCCTGC
AGGAGCGAAATCAGGAACAGGAAAAGGTAGAGCGACGCCGGCAGGTGCCCTTTCACCTGCACATCAACCT
GGAGCTGCTGGAGTGTGTCTATCTGGTGTCAGCTATGCTCCTGGAGATCCCCTACATGGCTGCCCATGAG
AGCGATGCCCGCCGACGCATGATCAGCAAGCAGTTCCACCACCAACTGCGGGTGGGCGAGCGGCAGCCCC
TGCTAGGTCCTCCCGAGTCAATGAGGGAGCATGTGGTCGCTGCCTCCAAGGCCATGAAGATGGGCGACTG
GAAGACCTGCCACAGTTTCATCATTAATGAAAAGATGAATGGGAAAGTGTGGGACCTTTTCCCTGAGGCT
GACAAAGTTCGCACCATGCTAGTTCGGAAGATCCAGGAAGAGTCTCTGAGGACCTACCTTTTTACCTACA
GCAGTGTCTATGACTCAATCAGTATGGAGACACTATCAGATATGTTTGAGCTGGATCTACCCACTGTTCA
CTCCATCATCAGCAAGATGATCATTAACGAAGAATTGATGGCTTCCCTGGACCAGCCGACACAGACTGTG
GTGATGCACCGTACTGAGCCCACTGCCCAGCAGAACTTGGCTCTGCAGCTGGCTGAGAAGCTTGGCAGCC
TAGTGGAGAATAATGAACGGGTGTTTGACCATAAGCAGGGAACCTATGGTGGCTATTTCCGAGACCAAAA
GGATGGCTACCGAAAAAATCTAGGCTATATGCGCCGTGGTGGCTACCGCCAGCAGCAGTCTCAGACAGCC
TAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211151 representing BC025032
Red=Cloning site Green=Tags(s)

MSRFFTTGSDSESESSLSGEELVTKPVSGNYGKQPLLLSEDEEDTKRVVRSAKDKRFEELTNLIRTIRNA
MKIRDVTKCLEEFELLGKAYGKAKSIVDKEGVPRFYIRILTDLEDYLNELWEDKEGKKKMNKNNAKALST
LRQKIRKYNRDFESHITNYKQNPEQSADEDAEKNEEDSEGSSDEDEDEDGVGNTTFLKKKQESSGESRKF
HKKMEDDDEDSEDSEDEEWDTSSTSSDSDSEEEEGKQTVLASKFLKKAPTTEEDKKAAEKKREDKAKKKH
DRKSKRLDEEEEDNEGGEWERVRGGVPLVKEKPKMFAKGTEITHAVVIKKLNEILQVRGKKGTDRATQIE
LLQLLVQIAAENNLGVGVIVKIKFNIIASLYDYNPNLATYMKPEMWQMCLDCINELMDTLVAHSNIFVGE
NILEESENLHNFDQPLRVRGCILTLVERMDEEFTKIMQNTDPHSQEYVEHLKDEAQVCAIIERVQRYLEE
KGTTEEICQIYLRRILHTYYKFDYKAHQRQLTPPEGSSKSEQDQAENEGEDSAVLMERLCKYIYAKDRTD
RIRTCAILCHIYHHALHSRWYQARDLMLMSHLQDNIQHADPPVQILYNRTMVQLGICAFRQGLTKDAHNA
LLDIQSSGRAKELLGQGLLLRSLQERNQEQEKVERRRQVPFHLHINLELLECVYLVSAMLLEIPYMAAHE
SDARRRMISKQFHHQLRVGERQPLLGPPESMREHVVAASKAMKMGDWKTCHSFIINEKMNGKVWDLFPEA
DKVRTMLVRKIQEESLRTYLFTYSSVYDSISMETLSDMFELDLPTVHSIISKMIINEELMASLDQPTQTV
VMHRTEPTAQQNLALQLAEKLGSLVENNERVFDHKQGTYGGYFRDQKDGYRKNLGYMRRGGYRQQQSQTA
Y

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC025032
ORF Size 2735 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC025032, AAH25032
RefSeq Size 2923 bp
RefSeq ORF 2735 bp
Locus ID 56347
Gene Summary Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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