Rbbp8 (BC050849) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	9930104E21Rik; CtIP; RBBP-8; RIM; SAE2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211085 representing BC050849 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATTTCAGGAAGCGGCTGTGGAAGCCCTAATTCTGCGGATGCATCCAATGACTTCAAGGAACTCT GGACAAAACTAAAAGAGTATCATGATAAGGAAGTGCAAGGTTTACAAGTCAAAGTAACCAAACTGAAAAA GGAACGAATTTTAGATGCACAGAGACTAGAAGAATTCTTCACCAAAAATCAACAGCTGAGAGACCAGCAG AAAGTCCTTCAGGAAACCATTAAAATTTTAGAAGATAGGTTAAGAGCAGGATTGTGTGATCGCTGTGCAG TAACTGAAGAGCATATGCACAAAAAGCAGCAGGAGTTTGAAAATATCCGACAGCAGAACCTTAAGCTTAT TACAGAGCTGATGAATGAAAAGAATACTCTTCAAGAAGAAAACAAAAAGCTTTCTGAACAACTGCAGCAG AAAATGGAAAATGGTCAACAGGATCAAGTAGCTGAGCTTGCATGTGAGGAAAACATTATTCCAGACTCAC CAGTAACATCCTTTTCCTTTTCTGGCATTAACCGGCTACGAAAGAAGGAGAATCTCCATGTCCGATACGT GGAACAAACACATACTAAGTTGGAGCGCTCTCTATGTACAAATGAATTAAGAAAAATATCTAAGGATTCA GCTCCTGCACCAGTTAACTCTGAGGAACATGAAATTCTCGTAGCTGACACTTGTGATCAAAATCACTCAC CACTGTCAAAAATATGTGAAACAAGCAGTTACCCCACTGATAAGACATCTTTTAATTTAGACACTGTTGT TGCTGAGACACTTGGACTTAATGGTCAAGAAGAATCTGAACCCCAAGGTCCCATGAGCCCTCTGGGAAGT GAACTCTACCACTGTCTCAAAGAAGATCACAAAAAACACCCTTTTATGGAATCTGCAAGAAGTAAGGAAG ATAGCTTAAGATTTTCAGACTCTGCTTCAAAGACACCTCCTCAAGAATTTACTACTCGGGCGTCATCTCC GGTATTTGGAGCTACCTCTACTGTGAAAGCTCATTTGGGTTTGAATACAAGTTTCTCCCCCTCTCTCTTA GACATTGGGAAAAAAAACCTTCTGAAGACAGCTCCCTTTAGCAACATTGCTGTTTCTAGATCAGAGAAAG TTAGATCCAAATCTGAGGATAATGCCCTTTTCACACAACATAGTCTTGGGTCAGAAGTGAAGGTCATTAG CCAGTCATTTTCTAGTAAACAGATACTTACAAATAAAACTGTAAGTGACTCTGTAGATGAACAGTGCAGT GCTGACCACATGAATACTACTGTTGCTGATAAATATTTGGTGCCTTTGAAATCCCTGGGAGGCAAAGCAT CCAAAAGGAAGAGGACTGAGGAGGAAAGTGAGCATGCAGTAAAGTGCCCCCAGGCCTGTTTTGATAAGGA AAATGCCCTTCCTTTCCCAATGGAGAATCAGTTCTCCATGAATGGAGACCATGTGATGGATAAACCTCTG GACCTGTCAGATCGGTTTGCAGCTACTCAGCGGCAAGAGAAGAACCACGGAAACGAGACTTCTAAAAACA AACTTAAGCAAGCCACTATTTACGAGGCTTTGAAGCCCATTCCAAAGGGCTCCTCCTCAGGCCGTAAAGC CTTGAGTGGGGACTGCATGCCAGCCAAAGACTCTTGGGAGACATACTGCTTACAGCCGAGGAGCCTCCAG TCCTCTAGTAAATTCTCTCCAGACCAAAAAACACCATTACAGATAAAAGAAGAAAATCCTGTCTTCAAAA CACCTCCATGTTCACAGGAAAGTTTGGAGACAGAGAATCTCTTTGGTGATGTGAAGGGTACTGGTTCTCT TGTGCCAACAAAAGTAAAGAGCAGAGCAGTCCATGGAGGATGTGAGCTTGCATCAGTTCTTCAGTTAAAC CCCTGTAGAGTTGCTAAAACAAAGGCTCTGCCAAGCAACCAAGATACGTCCTTTGAAAATATCCAGTGGA GTGTAGACCCAGGAGCAGACCTTTCTCAGTATAAAATGGATGTTACTGTAATAGATACAAAGGATAGCAG TCACTCACGATTAGGAGGAGAAACAGTGGACATGGACTGTACATTGGTCAGTGAAACCGTGCTTTTAAAA ATGAAGAAACAAGAGCAAAAAGAAAGAAGTCCAAATGGAGACATAAAAATGAATGATAGCCTAGAAGATA TGTTTGATCGGACAACACATGAAGAGTATGAGTCCTGTTTGGCAGATAGCTTCTCCCAGGTACCAGATGA GGAAGAACTGCCTGACACCACAAAGAAAACAAACATACCTGCTGATAAACAAGATGGAGTCAAACAGAAA GCATTTGTGGGGCCATATTTTAAAGATAAGGAGAGAGAAACTAGCATACAGAATTTTCCTCATATTGAGG TAGTTCGGAAAAAAGAAGAGAGAAGGAAATTGCTTGGGCACACATGTAAAGAATGTGAAATTTATTATGC AGATTTGCCAGCAGAAGAAAGAGAAAAGAAGTTGGCTTCCTGCTCAAGACACCGATTTCGCTACATTCCA CCCAACACACCAGAGAATTTCTGGGAAGTTGGTTTTCCTTCTACTCAGACGTGTCTGGAAAGAGGTTATA TCAAGGAAGATCTCGATCTTAGTCCTCGTCCCAAAAGACGGCAGCCTTACAACGCGGTCTTTTCTCCAAA AGGCAAAGAACAGAGGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211085 representing BC050849 Red=Cloning site Green=Tags(s) MSISGSGCGSPNSADASNDFKELWTKLKEYHDKEVQGLQVKVTKLKKERILDAQRLEEFFTKNQQLRDQQ KVLQETIKILEDRLRAGLCDRCAVTEEHMHKKQQEFENIRQQNLKLITELMNEKNTLQEENKKLSEQLQQ KMENGQQDQVAELACEENIIPDSPVTSFSFSGINRLRKKENLHVRYVEQTHTKLERSLCTNELRKISKDS APAPVNSEEHEILVADTCDQNHSPLSKICETSSYPTDKTSFNLDTVVAETLGLNGQEESEPQGPMSPLGS ELYHCLKEDHKKHPFMESARSKEDSLRFSDSASKTPPQEFTTRASSPVFGATSTVKAHLGLNTSFSPSLL DIGKKNLLKTAPFSNIAVSRSEKVRSKSEDNALFTQHSLGSEVKVISQSFSSKQILTNKTVSDSVDEQCS ADHMNTTVADKYLVPLKSLGGKASKRKRTEEESEHAVKCPQACFDKENALPFPMENQFSMNGDHVMDKPL DLSDRFAATQRQEKNHGNETSKNKLKQATIYEALKPIPKGSSSGRKALSGDCMPAKDSWETYCLQPRSLQ SSSKFSPDQKTPLQIKEENPVFKTPPCSQESLETENLFGDVKGTGSLVPTKVKSRAVHGGCELASVLQLN PCRVAKTKALPSNQDTSFENIQWSVDPGADLSQYKMDVTVIDTKDSSHSRLGGETVDMDCTLVSETVLLK MKKQEQKERSPNGDIKMNDSLEDMFDRTTHEEYESCLADSFSQVPDEEELPDTTKKTNIPADKQDGVKQK AFVGPYFKDKERETSIQNFPHIEVVRKKEERRKLLGHTCKECEIYYADLPAEEREKKLASCSRHRFRYIP PNTPENFWEVGFPSTQTCLERGYIKEDLDLSPRPKRRQPYNAVFSPKGKEQRT TRTRPLE - GFP Tag - V
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC050849
ORF Size	2679 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050849, AAH50849
RefSeq Size	3157 bp
RefSeq ORF	2681 bp
Locus ID	225182
Gene Summary	Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations.[UniProtKB/Swiss-Prot Function]

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