Tnpo1 (NM_001048267) Mouse Tagged ORF Clone
CAT#: MG211077
- TrueORF®
Tnpo1 (tGFP-tagged) - Mouse transportin 1 (Tnpo1), transcript variant 2
ORF Plasmid: DDK
"NM_001048267" in other vectors (4)
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CNY 9,690.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AU021749; D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG211077 representing NM_001048267
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTATGAGTGGAAACCTGACGAGCAAGGGCTTCAGCAGATCCTGCAGCTGCTCAAGGAGTCCCAGT CCCCAGACACCACCATCCAGAGGACCGTGCAACAAAAACTGGAACAACTCAATCAATATCCAGATTTTAA CAACTACTTGATTTTTGTTCTTACAAAATTAAAATCTGAAGATGAGCCCACACGGTCATTGAGCGGTCTG ATCTTGAAGAACAATGTGAAAGCTCATTTTCAGAACTTCCCAAATGGTGTGACAGACTTCATCAAGAGTG AATGTCTAAATAACATTGGGGACTCCTCTCCGTTGATCAGAGCCACTGTCGGTATTTTAATTACAACCAT AGCCTCCAAGGGAGAGCTGCAGAATTGGCCTGATCTCTTACCAAAACTCTGTAGCCTGCTGGATTCCGAG GACTACAACACTTGTGAGGGAGCCTTCGGTGCCCTTCAGAAGATATGTGAGGACTCTGCAGAGATTTTAG ACAGTGATGTCTTAGATCGCCCTCTCAACATCATGATCCCCAAGTTTTTACAGTTTTTCAAGCACAGCAG TCCCAAAATAAGGTCTCATGCTGTTGCATGTGTCAATCAGTTCATCATCAGTCGGACCCAGGCGCTCATG CTGCACATCGATTCCTTCATTGAGAACCTCTTTGCACTGGCTGGCGACGAGGAAGCAGAGGTGCGGAAGA ACGTGTGCCGGGCGCTTGTGATGTTGCTTGAAGTCCGGATGGATCGCCTGCTTCCTCATATGCACAACAT AGTCGAGTACATGCTGCAGAGAACCCAAGACCAAGATGAGAATGTAGCTCTGGAGGCCTGTGAATTCTGG CTGACTTTGGCTGAACAGCCAATATGCAAAGATGTACTTGTAAGGCATCTACCAAAGTTGATTCCTGTGT TAGTGAATGGCATGAAGTACTCAGATATAGATATTATCCTGCTTAAGGGTGATGTTGAGGAAGATGAGAC CATCCCGGATAGTGAGCAGGATATACGGCCGCGGTTTCATCGCTCGAGGACAGTGGCTCAGCAGCATGAG GAGGATGGGATTGAGGAGGAAGACGATGATGATGACGAAATTGATGATGATGACACGATTTCTGACTGGA ACCTGAGAAAGTGTTCTGCTGCTGCTCTCGATGTTCTCGCAAATGTTTATCGTGATGAGCTTTTGCCGCA CATTCTGCCACTTTTGAAAGAATTGCTTTTCCATCATGAATGGGTTGTGAAAGAATCTGGCATCTTGGTT TTAGGAGCAATTGCTGAAGGTTGTATGCAAGGCATGATTCCGTACCTGCCCGAGCTCATTCCTCACCTTA TTCAGTGCCTTTCTGATAAAAAGGCTCTTGTGCGTTCCATCACCTGCTGGACTCTTAGCCGCTATGCACA CTGGGTAGTCAGCCAGCCACCAGATACGTACCTGAAGCCATTAATGACAGAACTGCTGAAGCGTATCCTC GATAGCAACAAGAGAGTACAGGAAGCAGCTTGCAGTGCCTTCGCTACATTAGAAGAGGAGGCTTGTACAG AGCTCGTCCCTTACCTTGCTTATATACTCGATACCCTCGTCTTCGCCTTCAGTAAATACCAGCATAAGAA CCTGCTCATTCTGTACGACGCCATAGGGACACTGGCAGATTCAGTGGGACATCATTTAAACAAGCCAGAA TATATTCAGATGCTAATGCCTCCTTTGATCCAGAAATGGAACATGCTGAAGGATGAAGACAAGGATCTTT TCCCTTTGCTTGAGTGTCTCTCGTCTGTTGCCACAGCCTTGCAGTCTGGCTTCCTTCCATATTGTGAACC TGTATATCAGCGTTGCGTAAACCTAGTTCAGAAGACTCTGGCACAAGCCATGCTAAACAATGCTCAACCA GAACAGTATGAAGCTCCAGATAAAGATTTTATGATTGTGGCTCTTGACTTACTCAGCGGCCTGGCTGAGG GCCTGGGAGGCAACATTGAGCAGCTGGTGGCCCGCAGTAACATCCTGACGCTGATGTACCAGTGCATGCA GGATAAAATGCCCGAGGTTCGGCAGAGTTCTTTTGCATTGCTAGGTGACCTGACTAAAGCGTGCTTCCAG CATGTTAAGCCTTGTATAGCTGATTTCATGCCAATATTGGGAACCAATCTAAATCCAGAGTTTATTTCAG TCTGCAACAATGCCACCTGGGCGATTGGGGAAATATCAATCCAAATGGGTATAGAGATGCAGCCCTACAT CCCTATGGTGTTGCACCAGCTTGTGGAGATCATTAACAGACCCAACACCCCAAAGACGCTGTTGGAGAAC ACAGCAATAACAATTGGTCGTCTTGGTTACGTTTGTCCTCAAGAGGTGGCCCCCATGCTACAGCAGTTTA TAAGACCCTGGTGTACCTCTCTGAGAAACATAAGAGACAATGAAGAAAAAGATTCAGCATTCCGTGGGAT TTGTACCATGATCAGTGTGAATCCCAGTGGCGTAATCCAAGATTTTATATTTTTTTGTGATGCTGTTGCA TCATGGATTAACCCAAAAGATGATCTCAGAGACATGTTCTGTAAGATCCTTCATGGATTTAAAAACCAAG TTGGGGATGAAAATTGGAGGCGATTCTCTGACCAGTTTCCTCTTCCCTTAAAAGAGCGTCTTGCAGCTTT TTATGGTGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211077 representing NM_001048267
Red=Cloning site Green=Tags(s) MEYEWKPDEQGLQQILQLLKESQSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTRSLSGL ILKNNVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLPKLCSLLDSE DYNTCEGAFGALQKICEDSAEILDSDVLDRPLNIMIPKFLQFFKHSSPKIRSHAVACVNQFIISRTQALM LHIDSFIENLFALAGDEEAEVRKNVCRALVMLLEVRMDRLLPHMHNIVEYMLQRTQDQDENVALEACEFW LTLAEQPICKDVLVRHLPKLIPVLVNGMKYSDIDIILLKGDVEEDETIPDSEQDIRPRFHRSRTVAQQHE EDGIEEEDDDDDEIDDDDTISDWNLRKCSAAALDVLANVYRDELLPHILPLLKELLFHHEWVVKESGILV LGAIAEGCMQGMIPYLPELIPHLIQCLSDKKALVRSITCWTLSRYAHWVVSQPPDTYLKPLMTELLKRIL DSNKRVQEAACSAFATLEEEACTELVPYLAYILDTLVFAFSKYQHKNLLILYDAIGTLADSVGHHLNKPE YIQMLMPPLIQKWNMLKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQAMLNNAQP EQYEAPDKDFMIVALDLLSGLAEGLGGNIEQLVARSNILTLMYQCMQDKMPEVRQSSFALLGDLTKACFQ HVKPCIADFMPILGTNLNPEFISVCNNATWAIGEISIQMGIEMQPYIPMVLHQLVEIINRPNTPKTLLEN TAITIGRLGYVCPQEVAPMLQQFIRPWCTSLRNIRDNEEKDSAFRGICTMISVNPSGVIQDFIFFCDAVA SWINPKDDLRDMFCKILHGFKNQVGDENWRRFSDQFPLPLKERLAAFYGV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001048267 |
ORF Size | 2670 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001048267.1, NP_001041732.1 |
RefSeq Size | 5385 bp |
RefSeq ORF | 2673 bp |
Locus ID | 238799 |
UniProt ID | Q8BFY9 |
Gene Summary | Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:11493596). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A (By similarity). In vitro, mediates nuclear import of SRP19 (By similarity). Mediates the import of histones H2A, H2B, H3 and H4 (PubMed:11493596). Mediates nuclear import of ADAR/ADAR1 in a RanGTP-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202073 | Tnpo1 (untagged) - Mouse transportin 1 (Tnpo1), transcript variant 2, (10ug) |
CNY 9,784.00 |
|
MR211077 | Tnpo1 (Myc-DDK-tagged) - Mouse transportin 1 (Tnpo1), transcript variant 2 |
CNY 9,776.00 |
|
MR211077L3 | Lenti ORF clone of Tnpo1 (Myc-DDK-tagged) - Mouse transportin 1 (Tnpo1), transcript variant 2 |
CNY 10,740.00 |
|
MR211077L4 | Lenti ORF clone of Tnpo1 (mGFP-tagged) - Mouse transportin 1 (Tnpo1), transcript variant 2 |
CNY 10,740.00 |