Ppp1r10 (BC052059) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	CAT53, FB19, PNUTS
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211070 representing BC052059 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTCAGGTCCCATAGACCCCAAAGAACTGCTCAAGGGCCTGGATAGCTTCCTTACCCGGGACGGAG AAGTGAAGAGTGTGGATGGAATTTCCAAGATCTTCAGTCTAATGAAGGAGGCACGAAAGATGGTGAGTCG GTGCACGTACTTGAACATAATCCTGCAGACCCGCGCTCCAGAAGTGCTCGTCAAGTTTATTGATGTCGGT GGCTACAAGCTTCTGAACAACTGGCTAACATATTCAAAGACCACCAACAACATTCCTCTCCTACAGCAGA TTCTGCTGACTCTGCAGCACCTCCCGCTCACTGTGGACCATCTCAAGCAGAACAACACAGCAAAACTAGT GAAGCAGCTCAGCAAGTCAAGTGAGGATGAAGAGCTCCGGAAATTGGCATCAGTCCTTGTCAGTGACTGG ATGGCTGTCATCCGCTCCCAGAGTAGCACCCAGCCTGCAGAGAAAGATAAGAAGAAAAGGAAGGAAGAGG GGAAAAGTCGAACCACGCTTCCTGAGCGGCCTTTGACTGAAGTGAAGGCTGAGACCCGGGCTGAGGAAGC CCCAGAGAAGAAGAAAGAGAAGCCCAAGTCACTTAGAACTACGGCACCCAGTCATGCCAAGTTCCGTTCT ACTGGACTGGAGCTGGACACCCCATCTTTGGTGCCTGTGAAGAAGAACTCCAGCACTGTGGTTGTGTCAG ACAAGTACAACCTGAAGCCCATCCCCCTGAAGCGGCAGAGTGCCACAGCTGCTCCAGGTGACGCTGCCCC ACCTGCAGAGAAGAAATACAAGCCTCTCAACACAGCCCCCAATACCACCAAAGAAATCAAAGTGAAGATC ATCCCTCCACAGCCTATGGAGGGCCTGGGTTTTCTGGATGCTCTCAATTCAGCCCCTGTCCCAGGCATCA AAATTAAGAAGAAGAAGAAGGTACTATCGCCGACTGCTGCCAAGCCCAGCCCTTTTGAAGGGAAAACAAG CACCGAGCAGAGCACAGCCAAACCTTCCTCCCCAGAGCCTGCACCACCTGCTGAGCCCATGGACACGGAC CGCCCTGGCACCCCTGTTCCCCCTGTTGAAGTTCCAGAGCTCATGGATGCAGCCTCTTCAGAGCCAGGAG CTCTGGATGCAAAGCCTGTGGACAGCCCTGGAGATCCCAACCAGCTGACTCGCAAGGGCAGAAAGAGGAA AACTGTGACTTGGCCTGAGGAGGGCAAGCTGAGAGAGTATTTCTACTTTGAACTGGACGAAACTGAGCGA GTGAATGTGAACAAGATCAAAGACTTCGGGGAGGCTGCTAAGCGTGAGATACTGTCTGACCGACATGCTT TCGAGACAGCTCGGCGACTAAGCCATGACAATATGGAGGAGAAAGTGCCTTGGGTGTGTCCCAGGCCTCT GGTTCTGCCCTCACCTCTTGTCATCCCTGGAAGCAACAGCCAGGAGCGATACATCCAGGCAGAGCGGGAG AAGGGCATCCTTCAGGAACTCTTCCTGAACAAGGAGAGTCCACATGAACCTGATCCTGAGCCATATGAAC CCATTCCCCCAAAACTCATCCCCTTGGATGAGGAATGTGCCATGGATGAGACTCCATATGTCGAGACCCT GGAGCCTGGAGGGTCTGGTGGCTCACCTGATGGAGCAGGAGGCTCCAAGCTGCCTCCAGTTCTGGCCAAT CTTATGGGAAGCATGGGAGCTGGAAAGAGCCCCCAGGGCCCTGGAGGAGGCGGCATCAATGTTCAGGAGA TCCTCACTTCCATAATGGGCAGTCCAAACAGCCACCCTTCAGAGGAGCTGCTGAAGCAGCCTGACTACTC AGACAAGCTCAAGCAGATGCTGGTGCCTCATGGGCTCCTAGGTCCTGGTCCTGTGGCCAATGGCTTTCCA CCAGGAGGCCCTGGGGGCCCGAAGGGTATGCAGCATTTCCCCCCTGGTCCTGGAGGGCCCATGCCAGGTC CCCATGGAGGCCCTGGTGGACCAGTGGGTCCACGTCTCTTAGGTCCCCCACCACCTTCTCGGGGAGGTGA TCCATTCTGGGATGGCCCAGGTGATCCAATGCGAGGTGGCCCAATGCGTGGGGGTCCAGGACCTGCTCCC GGACCATACCACAGAGGCCGAGGGGGTCGAGGAGGAAATGAGCCACCGCCACCACCTCCATTCCGAGGAG CCAGAGGAGGTCGTTCCGGAGGAGGACCACCAAATGGCCGAGGGGGTCCTGGTGGAGGAGGCATGGTTGG AGGTGGTGGGCACCGCCCCCATGAAGGCCCTGGAGGGAGCATGGGCAGTGGACATCGTTCCCATGATGGC CCTGGAGGGAACATGGGCAGTGGACATCGTTCTCATGATGGCCCTGGAGGGAACATGGGTGGAAGTGGTG GACATCGTTCCCATGAAGGCCCTGGACACGGAGGACCCCATGGACACCGACCACATGATGTCCCTAGCCA TCGAGGCCACGACCATCGAGGGCCACCACCTCATGAACACCGTGGTCATGATGGCCATGGGGGAGGCGGC CACCGAGGGCATGATGGAGGCCACAGTCATGGAGGAGACATGTCAAACCGCCCTGTCTGCCGACACTTCA TGATGAAGGGCAACTGCCGCTATGAGAACAACTGTGCCTTCTACCACCCAGGGGTCAATGGGCCCCCACT GCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211070 representing BC052059 Red=Cloning site Green=Tags(s) MGSGPIDPKELLKGLDSFLTRDGEVKSVDGISKIFSLMKEARKMVSRCTYLNIILQTRAPEVLVKFIDVG GYKLLNNWLTYSKTTNNIPLLQQILLTLQHLPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDW MAVIRSQSSTQPAEKDKKKRKEEGKSRTTLPERPLTEVKAETRAEEAPEKKKEKPKSLRTTAPSHAKFRS TGLELDTPSLVPVKKNSSTVVVSDKYNLKPIPLKRQSATAAPGDAAPPAEKKYKPLNTAPNTTKEIKVKI IPPQPMEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAKPSPFEGKTSTEQSTAKPSSPEPAPPAEPMDTD RPGTPVPPVEVPELMDAASSEPGALDAKPVDSPGDPNQLTRKGRKRKTVTWPEEGKLREYFYFELDETER VNVNKIKDFGEAAKREILSDRHAFETARRLSHDNMEEKVPWVCPRPLVLPSPLVIPGSNSQERYIQAERE KGILQELFLNKESPHEPDPEPYEPIPPKLIPLDEECAMDETPYVETLEPGGSGGSPDGAGGSKLPPVLAN LMGSMGAGKSPQGPGGGGINVQEILTSIMGSPNSHPSEELLKQPDYSDKLKQMLVPHGLLGPGPVANGFP PGGPGGPKGMQHFPPGPGGPMPGPHGGPGGPVGPRLLGPPPPSRGGDPFWDGPGDPMRGGPMRGGPGPAP GPYHRGRGGRGGNEPPPPPPFRGARGGRSGGGPPNGRGGPGGGGMVGGGGHRPHEGPGGSMGSGHRSHDG PGGNMGSGHRSHDGPGGNMGGSGGHRSHEGPGHGGPHGHRPHDVPSHRGHDHRGPPPHEHRGHDGHGGGG HRGHDGGHSHGGDMSNRPVCRHFMMKGNCRYENNCAFYHPGVNGPPLP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC052059
ORF Size	2666 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC052059, AAH52059
RefSeq Size	4240 bp
RefSeq ORF	2666 bp
Locus ID	52040
Gene Summary	Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers.[UniProtKB/Swiss-Prot Function]

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