Tyro3 (BC066058) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Dtk, Rse, Sky, Tif, Etk-2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211051 representing BC066058 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGAGGCGGAGCATGGGGTGGCCGGGGCTCCGGCCGCTGCTGCTGGCGGGACTGGCTTCTCTGC TGCTCCCCGGGTCTGCGGCCGCAGGCCTGAAGCTCATGGGCGCCCCAGTGAAGATGACCGTGTCTCAGGG GCAGCCAGTGAAGCTCAACTGCAGCGTGGAGGGGATGGAGGACCCTGACATCCACTGGATGAAGGATGGC ACCGTGGTCCAGAATGCAAGCCAGGTGTCCATCTCCATCAGCGAGCACAGCTGGATTGGCTTACTCAGCC TAAAGTCAGTGGAACGGTCTGATGCTGGCCTGTACTGGTGCCAGGTGAAGGATGGGGAGGAAACCAAGAT TTCTCAGTCAGTATGGCTCACTGTCGAAGGTGTGCCATTCTTCACAGTGGAACCAAAAGATCTGGCGGTG CCACCCAATGCCCCTTTTCAGCTGTCTTGTGAGGCTGTGGGTCCTCCAGAACCCGTAACCATTTACTGGT GGAGAGGACTCACTAAGGTTGGGGGACCTGCTCCCTCTCCCTCTGTTTTAAATGTGACAGGAGTGACCCA GCGCACAGAGTTTTCTTGTGAAGCCCGCAACATAAAAGGCCTGGCCACTTCCCGACCAGCCATTGTTCGC CTTCAAGCACCGCCTGCAGCTCCTTTCAACACCACAGTAACAACGATCTCCAGCTACAACGCTAGCGTGG CCTGGGTGCCAGGTGCTGACGGCCTAGCTCTGCTGCATTCCTGTACTGTACAGGTGGCACACGCCCCAGG AGAATGGGAGGCCCTTGCTGTTGTGGTTCCTGTGCCACCTTTTACCTGCCTGCTTCGGAACTTGGCCCCT GCCACCAACTACAGCCTTAGGGTGCGCTGTGCCAATGCCTTGGGCCCTTCTCCCTACGGCGACTGGGTGC CCTTTCAGACAAAGGGCCTAGCGCCAGCCAGAGCTCCTCAGAATTTCCATGCCATTCGTACCGACTCAGG CCTTATCCTGGAATGGGAAGAAGTGATTCCTGAGGACCCTGGGGAAGGCCCCCTAGGACCTTATAAGCTG TCCTGGGTCCAAGAAAATGGAACCCAGGATGAGCTGATGGTGGAAGGGACCAGGGCCAATCTGACCGACT GGGATCCCCAGAAGGACCTGATTTTGCGTGTGTGTGCCTCCAATGCAATTGGTGATGGGCCCTGGAGTCA GCCACTGGTGGTGTCTTCTCATGACCATGCAGGGAGGCAGGGCCCTCCCCACAGCCGCACATCCTGGGTG CCTGTGGTCCTGGGCGTGCTCACCGCCCTGATCACAGCTGCTGCCTTGGCCCTCATCCTGCTTCGGAAGA GACGCAAGGAGACGCGTTTCGGGCAAGCCTTTGACAGTGTCATGGCCCGAGGGGAGCCAGCTGTACACTT CCGGGCAGCCCGATCTTTCAATCGAGAAAGGCCTGAACGCATTGAGGCCACATTGGATAGCCTGGGCATC AGCGATGAATTGAAGGAAAAGCTGGAGGATGTCCTCATTCCAGAGCAGCAGTTCACCCTCGGTCGGATGT TGGGCAAAGGAGAGTTTGGATCAGTGCGGGAAGCCCAGCTAAAGCAGGAAGATGGCTCCTTCGTGAAAGT GGCAGTGAAGATGCTGAAAGCTGACATCATTGCCTCAAGCGACATAGAAGAGTTCCTCCGGGAAGCAGCT TGCATGAAGGAGTTTGACCATCCACACGTGGCCAAGCTTGTTGGGGTGAGCCTCCGGAGCAGGGCTAAAG GTCGTCTCCCCATTCCCATGGTCATCCTGCCCTTCATGAAACATGGAGACTTGCACGCCTTTCTGCTCGC CTCCCGAATCGGGGAGAACCCTTTTAACCTGCCCCTCCAGACCCTGGTCCGGTTCATGGTGGACATTGCC TGTGGCATGGAGTACCTGAGCTCCCGGAACTTCATCCACCGAGACCTAGCAGCTCGGAATTGCATGCTGG CCGAGGACATGACAGTGTGTGTGGCTGATTTTGGACTCTCTCGGAAAATCTATAGCGGGGACTATTATCG TCAGGGCTGTGCCTCCAAATTGCCCGTCAAGTGGCTGGCCCTGGAGAGCTTGGCTGACAACTTGTATACT GTACACAGTGATGTGTGGGCCTTCGGGGTGACCATGTGGGAGATCATGACTCGTGGGCAGACGCCATATG CTGGCATTGAAAATGCCGAGATTTACAACTACCTCATCGGCGGGAACCGCCTGAAGCAGCCTCCGGAGTG CATGGAGGAAGTGTATGATCTCATGTACCAGTGCTGGAGCGCCGACCCCAAGCAGCGCCCAAGCTTCACG TGTCTGCGAATGGAACTGGAGAACATTCTGGGCCACCTGTCTGTGCTGTCCACCAGCCAGGACCCCTTGT ACATCAACATTGAGAGAGCTGAGCAGCCTACTGAGAGTGGCAGCCCTGAGGTCCACTGTGGAGAGCGATC CAGCAGCGAGGCAGGGGACGGCAGTGGCGTGGGGGCAGTAGGTGGCATCCCCAGTGACTCTCGGTACATC TTCAGCCCCGGAGGGCTATCCGAGTCACCAGGGCAGCTGGAGCAGCAGCCAGAAAGCCCCCTCAATGAGA ACCAGAGGCTGTTGTTGCTGCAGCAAGGGCTACTGCCTCACAGTAGCTGT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211051 representing BC066058 Red=Cloning site Green=Tags(s) MALRRSMGWPGLRPLLLAGLASLLLPGSAAAGLKLMGAPVKMTVSQGQPVKLNCSVEGMEDPDIHWMKDG TVVQNASQVSISISEHSWIGLLSLKSVERSDAGLYWCQVKDGEETKISQSVWLTVEGVPFFTVEPKDLAV PPNAPFQLSCEAVGPPEPVTIYWWRGLTKVGGPAPSPSVLNVTGVTQRTEFSCEARNIKGLATSRPAIVR LQAPPAAPFNTTVTTISSYNASVAWVPGADGLALLHSCTVQVAHAPGEWEALAVVVPVPPFTCLLRNLAP ATNYSLRVRCANALGPSPYGDWVPFQTKGLAPARAPQNFHAIRTDSGLILEWEEVIPEDPGEGPLGPYKL SWVQENGTQDELMVEGTRANLTDWDPQKDLILRVCASNAIGDGPWSQPLVVSSHDHAGRQGPPHSRTSWV PVVLGVLTALITAAALALILLRKRRKETRFGQAFDSVMARGEPAVHFRAARSFNRERPERIEATLDSLGI SDELKEKLEDVLIPEQQFTLGRMLGKGEFGSVREAQLKQEDGSFVKVAVKMLKADIIASSDIEEFLREAA CMKEFDHPHVAKLVGVSLRSRAKGRLPIPMVILPFMKHGDLHAFLLASRIGENPFNLPLQTLVRFMVDIA CGMEYLSSRNFIHRDLAARNCMLAEDMTVCVADFGLSRKIYSGDYYRQGCASKLPVKWLALESLADNLYT VHSDVWAFGVTMWEIMTRGQTPYAGIENAEIYNYLIGGNRLKQPPECMEEVYDLMYQCWSADPKQRPSFT CLRMELENILGHLSVLSTSQDPLYINIERAEQPTESGSPEVHCGERSSSEAGDGSGVGAVGGIPSDSRYI FSPGGLSESPGQLEQQPESPLNENQRLLLLQQGLLPHSSC SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC066058
ORF Size	2642 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC066058, AAH66058
RefSeq Size	4005 bp
RefSeq ORF	2642 bp
Locus ID	22174
Gene Summary	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.[UniProtKB/Swiss-Prot Function]

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