Tyro3 (BC066058) Mouse Tagged ORF Clone

CAT#: MG211051

  • TrueORF®

Tyro3 (tGFP-tagged) - Mouse TYRO3 protein tyrosine kinase 3 (cDNA clone MGC:90132 IMAGE:6834015)



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Mouse Protein / Lysate Request

CNY 8,460.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Dtk, Rse, Sky, Tif, Etk-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211051 representing BC066058
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGAGGCGGAGCATGGGGTGGCCGGGGCTCCGGCCGCTGCTGCTGGCGGGACTGGCTTCTCTGC
TGCTCCCCGGGTCTGCGGCCGCAGGCCTGAAGCTCATGGGCGCCCCAGTGAAGATGACCGTGTCTCAGGG
GCAGCCAGTGAAGCTCAACTGCAGCGTGGAGGGGATGGAGGACCCTGACATCCACTGGATGAAGGATGGC
ACCGTGGTCCAGAATGCAAGCCAGGTGTCCATCTCCATCAGCGAGCACAGCTGGATTGGCTTACTCAGCC
TAAAGTCAGTGGAACGGTCTGATGCTGGCCTGTACTGGTGCCAGGTGAAGGATGGGGAGGAAACCAAGAT
TTCTCAGTCAGTATGGCTCACTGTCGAAGGTGTGCCATTCTTCACAGTGGAACCAAAAGATCTGGCGGTG
CCACCCAATGCCCCTTTTCAGCTGTCTTGTGAGGCTGTGGGTCCTCCAGAACCCGTAACCATTTACTGGT
GGAGAGGACTCACTAAGGTTGGGGGACCTGCTCCCTCTCCCTCTGTTTTAAATGTGACAGGAGTGACCCA
GCGCACAGAGTTTTCTTGTGAAGCCCGCAACATAAAAGGCCTGGCCACTTCCCGACCAGCCATTGTTCGC
CTTCAAGCACCGCCTGCAGCTCCTTTCAACACCACAGTAACAACGATCTCCAGCTACAACGCTAGCGTGG
CCTGGGTGCCAGGTGCTGACGGCCTAGCTCTGCTGCATTCCTGTACTGTACAGGTGGCACACGCCCCAGG
AGAATGGGAGGCCCTTGCTGTTGTGGTTCCTGTGCCACCTTTTACCTGCCTGCTTCGGAACTTGGCCCCT
GCCACCAACTACAGCCTTAGGGTGCGCTGTGCCAATGCCTTGGGCCCTTCTCCCTACGGCGACTGGGTGC
CCTTTCAGACAAAGGGCCTAGCGCCAGCCAGAGCTCCTCAGAATTTCCATGCCATTCGTACCGACTCAGG
CCTTATCCTGGAATGGGAAGAAGTGATTCCTGAGGACCCTGGGGAAGGCCCCCTAGGACCTTATAAGCTG
TCCTGGGTCCAAGAAAATGGAACCCAGGATGAGCTGATGGTGGAAGGGACCAGGGCCAATCTGACCGACT
GGGATCCCCAGAAGGACCTGATTTTGCGTGTGTGTGCCTCCAATGCAATTGGTGATGGGCCCTGGAGTCA
GCCACTGGTGGTGTCTTCTCATGACCATGCAGGGAGGCAGGGCCCTCCCCACAGCCGCACATCCTGGGTG
CCTGTGGTCCTGGGCGTGCTCACCGCCCTGATCACAGCTGCTGCCTTGGCCCTCATCCTGCTTCGGAAGA
GACGCAAGGAGACGCGTTTCGGGCAAGCCTTTGACAGTGTCATGGCCCGAGGGGAGCCAGCTGTACACTT
CCGGGCAGCCCGATCTTTCAATCGAGAAAGGCCTGAACGCATTGAGGCCACATTGGATAGCCTGGGCATC
AGCGATGAATTGAAGGAAAAGCTGGAGGATGTCCTCATTCCAGAGCAGCAGTTCACCCTCGGTCGGATGT
TGGGCAAAGGAGAGTTTGGATCAGTGCGGGAAGCCCAGCTAAAGCAGGAAGATGGCTCCTTCGTGAAAGT
GGCAGTGAAGATGCTGAAAGCTGACATCATTGCCTCAAGCGACATAGAAGAGTTCCTCCGGGAAGCAGCT
TGCATGAAGGAGTTTGACCATCCACACGTGGCCAAGCTTGTTGGGGTGAGCCTCCGGAGCAGGGCTAAAG
GTCGTCTCCCCATTCCCATGGTCATCCTGCCCTTCATGAAACATGGAGACTTGCACGCCTTTCTGCTCGC
CTCCCGAATCGGGGAGAACCCTTTTAACCTGCCCCTCCAGACCCTGGTCCGGTTCATGGTGGACATTGCC
TGTGGCATGGAGTACCTGAGCTCCCGGAACTTCATCCACCGAGACCTAGCAGCTCGGAATTGCATGCTGG
CCGAGGACATGACAGTGTGTGTGGCTGATTTTGGACTCTCTCGGAAAATCTATAGCGGGGACTATTATCG
TCAGGGCTGTGCCTCCAAATTGCCCGTCAAGTGGCTGGCCCTGGAGAGCTTGGCTGACAACTTGTATACT
GTACACAGTGATGTGTGGGCCTTCGGGGTGACCATGTGGGAGATCATGACTCGTGGGCAGACGCCATATG
CTGGCATTGAAAATGCCGAGATTTACAACTACCTCATCGGCGGGAACCGCCTGAAGCAGCCTCCGGAGTG
CATGGAGGAAGTGTATGATCTCATGTACCAGTGCTGGAGCGCCGACCCCAAGCAGCGCCCAAGCTTCACG
TGTCTGCGAATGGAACTGGAGAACATTCTGGGCCACCTGTCTGTGCTGTCCACCAGCCAGGACCCCTTGT
ACATCAACATTGAGAGAGCTGAGCAGCCTACTGAGAGTGGCAGCCCTGAGGTCCACTGTGGAGAGCGATC
CAGCAGCGAGGCAGGGGACGGCAGTGGCGTGGGGGCAGTAGGTGGCATCCCCAGTGACTCTCGGTACATC
TTCAGCCCCGGAGGGCTATCCGAGTCACCAGGGCAGCTGGAGCAGCAGCCAGAAAGCCCCCTCAATGAGA
ACCAGAGGCTGTTGTTGCTGCAGCAAGGGCTACTGCCTCACAGTAGCTGT


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211051 representing BC066058
Red=Cloning site Green=Tags(s)

MALRRSMGWPGLRPLLLAGLASLLLPGSAAAGLKLMGAPVKMTVSQGQPVKLNCSVEGMEDPDIHWMKDG
TVVQNASQVSISISEHSWIGLLSLKSVERSDAGLYWCQVKDGEETKISQSVWLTVEGVPFFTVEPKDLAV
PPNAPFQLSCEAVGPPEPVTIYWWRGLTKVGGPAPSPSVLNVTGVTQRTEFSCEARNIKGLATSRPAIVR
LQAPPAAPFNTTVTTISSYNASVAWVPGADGLALLHSCTVQVAHAPGEWEALAVVVPVPPFTCLLRNLAP
ATNYSLRVRCANALGPSPYGDWVPFQTKGLAPARAPQNFHAIRTDSGLILEWEEVIPEDPGEGPLGPYKL
SWVQENGTQDELMVEGTRANLTDWDPQKDLILRVCASNAIGDGPWSQPLVVSSHDHAGRQGPPHSRTSWV
PVVLGVLTALITAAALALILLRKRRKETRFGQAFDSVMARGEPAVHFRAARSFNRERPERIEATLDSLGI
SDELKEKLEDVLIPEQQFTLGRMLGKGEFGSVREAQLKQEDGSFVKVAVKMLKADIIASSDIEEFLREAA
CMKEFDHPHVAKLVGVSLRSRAKGRLPIPMVILPFMKHGDLHAFLLASRIGENPFNLPLQTLVRFMVDIA
CGMEYLSSRNFIHRDLAARNCMLAEDMTVCVADFGLSRKIYSGDYYRQGCASKLPVKWLALESLADNLYT
VHSDVWAFGVTMWEIMTRGQTPYAGIENAEIYNYLIGGNRLKQPPECMEEVYDLMYQCWSADPKQRPSFT
CLRMELENILGHLSVLSTSQDPLYINIERAEQPTESGSPEVHCGERSSSEAGDGSGVGAVGGIPSDSRYI
FSPGGLSESPGQLEQQPESPLNENQRLLLLQQGLLPHSSC

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN BC066058
ORF Size 2642 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC066058, AAH66058
RefSeq Size 4005 bp
RefSeq ORF 2642 bp
Locus ID 22174
Gene Summary Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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